Mathematical tools for recovery of the load on the fissure according to the micro-CT results.

In the present paper X-ray microtomographic research of a molar tooth was conducted. The study revealed regions with a reduced mineral density in the vicinity of the fissure tip. The basic assumption investigated is that corrosion induced enamel mineral density decrease is enhanced by high tensile stresses generated by mechanical load on the occlusal surface of the tooth during crushing of food. Magnitude and location of tensile stress concentration occurs at the fissure tip and may be determined by solving the problem of the stress-strain state of the tooth crown enamel with a wedge-shaped notch. The study of stresses in the vicinity of fissure tip make it possible to construct the boundaries of enhanced enamel virtual fracture. Comparison of the sizes and locations of areas with a reduced enamel mineral density with the sizes and locations of areas of virtual enamel fracture made it possible to establish their approximate congruence. This circumstance made it possible to recreate by mathematical means the nature and magnitude of the force load on the lateral surface of the fissure. Degree of influence of the main parameters of the fissure on the geometrical characteristics of the virtual fracture, such as its area and diameters, were determined.

X-ray micro-computed tomography in the assessment of penile cavernous fibrosis in a rabbit castration model.

BACKGROUND: Current assessment methods of penile cavernous fibrosis in animal models have limitations due to the inability to provide complex and volume analysis of fibrotic alterations. OBJECTIVE: The aim was to evaluate micro-computed tomography for assessment of cavernous fibrosis and compare it with histological, histochemical, immunohistochemical, and RT-PCR analysis. MATERIALS AND METHODS: A controlled trial was performed involving 25 New Zealand male rabbits with induced testosterone deficiency by orchidectomy. Penile samples were obtained before and after 7, 14, 21, and 84 days from orchidectomy. We consistently performed (a) gray value analysis of corpora cavernosa 3D models reconstructed after micro-computed tomography, (b) morphometry of smooth muscles/connective tissue ratio, collagen type I/III ratio, and area of TGF-beta-1 expression in corpora cavernosa, and (c) RT-PCR of TGF-beta-1 expression. RESULTS: Micro-computed tomography allowed visualization of penile structures at a resolution comparable to light microscopy. Gray values of corpora cavernosa decreased from 1673 (1512-1773) on the initial day to 1184 (1089-1232) on the 21st day (p < 0.005). However, on the 84th day, it increased to 1610 (1551-1768). On 21st and 84th days, there was observed a significant decrease in smooth muscle/connective tissue ratio and a significant increase in collagen type I/III ratio (p < 0.05). TGF-beta1 expression increased on the 84th day according to immunohistochemistry (p < 0.005). RT-PCR was impossible to conduct due to the absence of RNA in obtained samples after micro-CT. DISCUSSION AND CONCLUSIONS: Micro-computed tomography provided 3D visualization of entire corpora cavernosa and assessment of radiodensity alterations by gray value analysis in fibrosis progression. We speculate that gray value changes at early and late fibrosis stages could be related to tissue reorganization. RT-PCR is impossible to conduct on tissue samples studied by micro-CT due to RNA destruction. We also suggest that micro-computed tomography could negatively affect the immunohistochemical outcome, as a significant increase of TGF-beta-1 expression occurs later than histological fibrotic signs.

Wear of Functionally Graded Coatings under Frictional Heating Conditions.

Multilayered and functionally graded coatings are extensively used for protection against wear of the working surfaces of mechanisms and machines subjected to sliding contact. The paper considers the problem of wear of a strip made of a functionally graded material, taking into account the heating of the sliding contact from friction. Wear is modeled by a moving strip along the surface of a hard abrasive in the form of a half-plane. With the help of the integral Laplace transform with respect to time, the solutions are constructed as convolutions from the law of the introduction of an abrasive into the strip and the original in the form of a contour integral of the inverse Laplace transform. The study of the integrands of contour quadratures in the complex plane allowed determination of the regions of stable solutions to the problem. Unstable solutions of the problem lead to the concept of thermoelastic instability of the contact with friction and formed regions of unstable solutions. The solutions obtained made it possible to determine a formula for the coefficient of functionally graded inhomogeneity of the coating material and to study its effect on the occurrence of thermoelastic instability of the contact taking friction into account, as well as on its main characteristics: temperature, displacement, stress and wear of the functionally graded material of the coating. The effects of the abrasive speed, contact stresses and temperature on wear of the coating with the functionally graded inhomogeneity of the material by the depth were investigated.

Visualization of different anatomical parts of the enucleated human eye using X-ray micro-CT imaging.

Micro-CT visualization allows reconstruction of eye structures with the resolution of light microscopy and estimation of tissue densities. Moreover, this method excludes damaging procedures and allows further histological staining due to the similar steps in the beginning. We have shown the feasibility of the lab-based micro-CT machine usage for visualization of clinically important compartments of human eye such as trabecular outflow pathway, retina, iris and ciliary body after pre-treatment with iodine in ethanol. We also identified the challenges of applying this contrasting technique to lens, cornea, and retina and proposed alternative staining methods for these tissues. Thereby this work provides a starting point for other studies for imaging of human eyes in normal and pathological conditions using lab-based micro-CT systems.