Zeolite-based, dry formulations for conservation and practical application of Paraburkholderia phytofirmans PsJN.

AIMS: Producing dry, zeolite-based granular and powder inoculants of the Gram-negative, plant growth-promoting bacterium Paraburkholderia phytofirmans PsJN. Key aspects were maintenance of cell viability during desiccation and throughout storage at ambient conditions. METHODS AND RESULTS: Twenty additives and exopolysaccharide (EPS) produced by PsJN were screened for conserving cell viability of PsJN in air-drying and lyophilization. Suitable combinations (e.g. skimmed milk + air-drying) increased survival of PsJN up to 100 000-fold and maintained it for >7 months. EPS performed as good as skimmed milk during air-drying, but was second-rank regarding shelf life. Combinations of zeolite, skimmed milk and gelatin as a film-forming agent were extruded and processed into granules and powders, both displaying relatively stable viability for over 4 weeks at ambient conditions. Gelatin promoted brittleness of zeolite-based inoculants. CONCLUSIONS: Viability of highly sensitive PsJN was successfully conserved in dry formulations, taking into account the interplay between carrier, protectants, drying method and coating agent. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study to provide ways of maintaining viability of PsJN during desiccation stress and to investigate the applicability of its EPS as a protectant, thus ultimately facilitating successful plant inoculation especially under field conditions.

Functional characteristics of an endophyte community colonizing rice roots as revealed by metagenomic analysis.

Roots are the primary site of interaction between plants and microorganisms. To meet food demands in changing climates, improved yields and stress resistance are increasingly important, stimulating efforts to identify factors that affect plant productivity. The role of bacterial endophytes that reside inside plants remains largely unexplored, because analysis of their specific functions is impeded by difficulties in cultivating most prokaryotes. Here, we present the first metagenomic approach to analyze an endophytic bacterial community resident inside roots of rice, one of the most important staple foods. Metagenome sequences were obtained from endophyte cells extracted from roots of field-grown plants. Putative functions were deduced from protein domains or similarity analyses of protein-encoding gene fragments, and allowed insights into the capacities of endophyte cells. This allowed us to predict traits and metabolic processes important for the endophytic lifestyle, suggesting that the endorhizosphere is an exclusive microhabitat requiring numerous adaptations. Prominent features included flagella, plant-polymer-degrading enzymes, protein secretion systems, iron acquisition and storage, quorum sensing, and detoxification of reactive oxygen species. Surprisingly, endophytes might be involved in the entire nitrogen cycle, as protein domains involved in N(2)-fixation, denitrification, and nitrification were detected and selected genes expressed. Our data suggest a high potential of the endophyte community for plant-growth promotion, improvement of plant stress resistance, biocontrol against pathogens, and bioremediation, regardless of their culturability.