[Preparation of DNA-duplexes, containing internucleotide thiophosphate groups in various positions of the recognition site for the EcoRII restriction endonuclease]. / Poluchenie DNK-dupleksov, soderzhashchikh v razlichnykh polozheniiakh uchastka uznavaniia éndonukleazy restriktsii EcoRII khiral'nye mezhnukleotidnye tioforfatnye gruppy.

Twenty-four 12-mer DNA duplexes, each containing a chiral phosphorothioate group successively replacing one of the internucleotide phosphate groups either in the EcoRII recognition site (5'CCA/TGG) or near to it, were obtained for studying the interaction of the restriction endonuclease EcoRII with internucleotide DNA phosphates. Twelve of the 12-mer oligonucleotides were synthesized as Rp and Sp diastereomeric mixtures. Six of them were separated by reversed-phase HPLC using various buffers. Homogeneous diastereomers of the other oligonucleotides were obtained by enzymatic ligation of the Rp and Sp diastereomers of 5- to 7-mer oligonucleotides preliminarily separated by HPLC with the corresponding short oligonucleotides on a complementary DNA template. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2003, vol. 29, no. 6; see also http://www.maik.ru.

[A comparative evaluation of the results of classical x-ray study and computed tomography in patients with exogenous allergic alveolitis]. / Sravnitel'naia otsenka rezul'tatov klassicheskogo rentgenologicheskogoissledovaniia i komp'iuternoi tomografii u bol'nykh ékzogennym allergicheskim al'veolitom.

A correlative study of the results of x-ray investigation using routine methods and computerized tomography (CT) was conducted to specify the roentgenomorphological substrate of changes in patients with exogenous allergic alveolitis. The established complex of routine methods is informative enough to interpret the revealed changes. However, at early stages CT helps to specify semiotics and permits obtaining additional information, particularly on quantitative, i.e. densitometric changes. In diffuse and disseminated pulmonary lesions CT can be used as an additional method.

[Simultaneous detection of T-, B- and D-rosette forming lymphocytes and null cells in humans]. / Odnovremennoe vyaiavlenie T-, B- i D-rozetkoobrazuiushchikh limfotsitov i nulevykh kletok cheloveka.

For the identification of T-, B-, and D-rosette-forming lymphocytes and the "null" cells in the human peripheral blood a simultaneous reaction of rosette-forming cells with the use of zymosan-complement, sheep red blood cells, fixation and staining of smears in extraction of the lymphocytes tested with the aid of verographin may be recommended.

[Simultaneous determination of T-, B- and mixed rosette-forming lymphocytes in human peripheral blood]. / Odnovremenooe opredelenie T-, B- i smeshannykh rozetkoobrazuiushchikh limfotsitov v perifericheskoi krovi cheloveka.

Preliminary treatment of erythrocyte suspension with trypan blue solution did not affect their capacity to participate in the reaction of spontaneous rosette formation, but permitted to differentiate them in a mixture with other erythrocytes after staining with methyl-green-pyronine on a fixed slide. By using a mixture of such marked erythrocytes sensitized with antibodies and a complement, the presence in the peripheral blood of healthy people of a small lymphocyte subpopulation connecting these two types of indicator particles was demonstrated.