Effects of domestication and captive breeding on reaction to moving objects: implications for avoidance behaviours of masu salmon Oncorhynchus masou.

Domestication and captive breeding can compromise the obstacle- and predator-avoidance capabilities of animals in the wild. Whereas previous studies only examined these effects in combination, here we examine them individually by comparing the abilities of wild, F1 (offspring of wild parents) and captive-bred (approx. F15) masu salmon Oncorhynchus masou to avoid a falling object under experimental conditions. Rates of avoidance failure were low (wild, 12.5%; F1, 10.7%; captive-bred, 8%) under light conditions, but increased under dark conditions (wild, 11.1%; F1, 32.1%; captive-bred, 60.0%). We attribute the elevated avoidance failure rate among F1 fish to the lack of learning opportunities in hatchery environments (i.e. domestication), and the further elevation of avoidance failure rate among captive-bred fish to the degradation of sensory organ function (i.e. captive breeding). These results imply reduced survival rates for F1 and captive-bred fish in the wild and are consistent with the low stocking efficiencies reported for captive-bred masu salmon.

Domestication of captive-bred masu salmon Oncorhynchus masou masou (Salmonidae) leads to a significant decrease in numbers of lateral line organs.

Because captive-bred animals gradually adapt to artificial rearing environments due to evolving life history traits, such individuals sometimes show lessened performance in natural environments. The lateral line system, one of the principal sensory organs of fishes, varies according to habitat environments, sometimes differing even within the same species. A reduction in lateral line elements may also occur in successive generations of captive-bred fish. Such a reduction, involving neuromasts over the entire body, was examined for the first time in captive-bred masu salmon Oncorhynchus masou masou. The total number of neuromasts in captive-bred fish was ca. 10% lower than in wild-caught and F1 fishes, suggesting that the system in captive-bred fish had reduced in number due to domestication. Furthermore, differences in total neuromast numbers between captive-bred and wild fish were greater than between anadromous and fluvial populations of the species. The lower number of neuromasts could be one of the reasons behind the lower survival of captive-bred fish in natural environments.

Comparison of 137Cs uptake, depuration and continuous uptake, originating from feed, in five salmonid fish species.

The origin of 137Cs contamination, contamination levels, and its effective ecological half-life can differ among fish species, but until recently it was unknown whether interspecific differences in radiocaesium metabolism existed. We compare the trophic transfer of 137Cs in five salmonid species under controlled conditions. Fish were fed a diet containing 200 Bq kg-1 wet wt concentration of 137Cs for a fixed period of time. While there were almost no differences in contamination levels among individual fish within a species during fixed periods, the rate of 137Cs uptake was statistically different among the species examined, for which the effective half-life of 137Cs ranged 49-84 days. No significant difference in rate of decline in the quantity of 137Cs was apparent among species in two experiments after correcting for differences in growth. The effective half-life was shortest in Oncorhynchus mykiss and O. masou-two species exhibiting the best growth rate in both experiments-indicating an influence of the dilution effect associated with growth. When continuously fed 200 Bq kg-1 wet wt concentration of 137Cs for a period of 209-294 days, contamination levels in the five species ranged 216-240 Bq kg-1 wet wt, and reached a steady state value. Our results indicate metabolic rate, although accompanying a change of body size, did not affect 137Cs contamination levels in fish, which implies that contamination levels in the diet strongly determined contamination levels in fish.

Down-regulation of corticosteroid receptor in leucocytes of stressed rainbow trout.

The relationship between stress and immunosuppression was investigated in peripheral blood leucocytes (PBL) in rainbow trout, with reference to corticosteroid receptor (CR) expression and responses to cortisol- and/or lipopolysaccharide (LPS)-administration. Confinement stress in shallow water resulted in a sustained elevation of plasma cortisol, whereas lysozyme and immunoglobin levels were suppressed. Significant increases in mRNA levels of caspase-6 and insulin-like growth factor (IGF)-I were observed in PBL isolated from stressed fish. Confinement stress also suppressed proinflammatory cytokine, interleukin (IL)-1ß, expression in PBL. There were decreasing tendencies for the mRNA levels of CRs in PBL of stressed fish. In-vitro treatment of cortisol and LPS on isolated PBL from unstressed trout increased both IL-1 ß and CR mRNA expression. However, in PBL from stressed fish, cortisol and LPS treatment increased IL-1 ß but not CR mRNA levels. Proliferative activities estimated as in-vitro incorporation of bromodeoxyuridine (BrdU) were decreased by cortisol in PBL from the unstressed and stressed fish groups; however, LPS-stimulated proliferation was observed only in the unstressed fish. Ratios of apoptotic PBL quantified as cell fragmentation using an automated cell counter were increased by cortisol in both groups; however, LPS-stimulated apoptosis was observed only in the stressed fish. Our study reveals cortisol has immune-suppressive effects in stressed fish, irrespective of CR down-regulation and desensitization. The complexity of immune-endocrine interaction is shown by the stress-induced attenuation of LPS effects.