RESUMO
Land snails are exposed to conditions of high ambient temperature and low humidity, and their survival depends on a suite of morphological, behavioral, physiological, and molecular adaptations to the specific microhabitat. We tested in six populations of the land snail Theba pisana whether adaptations to different habitats affect their ability to cope with thermal stress and their strategies of heat shock protein (HSP) expression. Levels of Hsp70 and Hsp90 in the foot tissue were measured in field-collected snails and after acclimation to laboratory conditions. Snails were also exposed to various temperatures (32 up to 54 °C) for 2 h and HSP messenger RNA (mRNA) levels were measured in the foot tissue and survival was determined. To test whether the physiological and molecular data are related to genetic parameters, we analyzed T. pisana populations using partial sequences of nuclear and mitochondrial DNA ribosomal RNA genes. We show that populations collected from warmer habitats were more thermotolerant and had higher constitutive levels of Hsp70 isoforms in the foot tissue. Quantitative real-time polymerase chain reaction (PCR) analysis indicated that hsp70 and hsp90 mRNA levels increased significantly in response to thermal stress, although the increase in hsp70 mRNA was larger compared to hsp90 and its induction continued up to higher temperatures. Generally, warm-adapted populations had higher temperatures of maximal induction of hsp70 mRNA synthesis and higher upper thermal limits to HSP mRNA synthesis. Our study suggests that Hsp70 in the foot tissue of T. pisana snails may have important roles in determining stress resistance, while Hsp90 is more likely implicated in signal transduction processes that are activated by stress. In the phylogenetic analysis, T. pisana haplotypes were principally divided into two major clades largely corresponding to the physiological ability to withstand stress, thus pointing to genetically fixed tolerance.
Assuntos
Proteínas de Choque Térmico HSP70/genética , Proteínas de Choque Térmico HSP90/genética , Caramujos/genética , Aclimatação , Animais , Ecossistema , Proteínas de Choque Térmico HSP70/análise , Proteínas de Choque Térmico HSP90/análise , Resposta ao Choque Térmico , Temperatura Alta , Filogenia , RNA Mensageiro/análise , RNA Mensageiro/genética , Caramujos/química , Caramujos/fisiologia , Temperatura , Regulação para CimaRESUMO
Land snails frequently encounter desiccating conditions, and their survival depends on a suite of morphological, physiological, and molecular adaptations to the specific microhabitat. Strategies of survival can be determined by integrating information from various levels of biological organization. In this study, we used a combination of physiological parameters related to water economy and molecular factors (stress protein expression) to investigate the strategies of survival adopted by seven populations of the Mediterranean-type land snail Theba pisana from different habitats. We analyzed water compartmentalization during aestivation and used experimental desiccation to compare desiccation resistance. We also measured the endogenous levels of heat shock proteins (HSPs) Hsp72, Hsp74, and Hsp90 under nonstress conditions and analyzed the HSP response to desiccation in two populations that differed mostly in their resistance to desiccation. We revealed significant intraspecific differences in resistance to desiccation that seem to be determined by the speed of recruitment of the water-preserving mechanisms. The ability to cope with desiccating conditions was correlated with habitat temperature but not with the rainfall gradient, implying that in the coastal region, temperature is likely to have a major impact on desiccation resistance rather than precipitation. Also, higher desiccation resistance was correlated with higher constitutive levels of Hsp74 in the foot tissue. HSPs were upregulated during desiccation, but the response was delayed and was milder in the most resistant population compared to the most susceptible one. Our study suggests that T. pisana populations from warmer habitats were more resistant to desiccation and developed distinct strategies of HSP expression for survival, namely, the maintenance of high constitutive levels of Hsp70 together with a delayed and limited response to stress.
Assuntos
Clima , Proteínas de Choque Térmico/metabolismo , Caramujos/metabolismo , Animais , Dessecação , Ecossistema , Estivação , Proteínas de Choque Térmico HSP70/metabolismo , Proteínas de Choque Térmico HSP72/metabolismo , Proteínas de Choque Térmico HSP90/metabolismo , Israel , Estresse Fisiológico , TemperaturaRESUMO
Land snails are subject to daily and seasonal variations in temperature and in water availability, and use heat shock proteins (HSPs) as part of their survival strategy. We used experimental heat stress to test whether adaptation to different habitats affects HSP expression in two closely related Sphincterochila snail species, a desert species, Sphincterochila zonata, and a Mediterranean-type species, Sphincterochila cariosa. Our findings show that in S. cariosa, heat stress caused rapid induction of Hsp70 proteins and Hsp90 in the foot and kidney tissues, whereas the desert-inhabiting species S. zonata displayed delayed induction of Hsp70 proteins in the foot and upregulation of Hsp90 alone in the kidney. Our study suggests that Sphincterochila species use HSPs as part of their survival strategy following heat stress and that adaptation to different habitats results in the development of distinct strategies of HSP expression in response to heat, namely the reduced induction of HSPs in the desert-dwelling species. We suggest that the desert species S. zonata relies on mechanisms and adaptations other than HSP induction, thus avoiding the fitness consequences of continuous HSP upregulation.
Assuntos
Ecossistema , Resposta ao Choque Térmico/fisiologia , Caramujos/metabolismo , Animais , Proteínas de Choque Térmico HSP70/metabolismo , Proteínas de Choque Térmico HSP90/metabolismo , Rim/metabolismo , Estresse Fisiológico , TemperaturaRESUMO
Polmunate land snails are subject to stress conditions in their terrestrial habitat, and depend on a range of behavioural, physiological and biochemical adaptations for coping with problems of maintaining water, ionic and thermal balance. The involvement of the heat shock protein (HSP) machinery in land snails was demonstrated following short-term experimental aestivation and heat stress, suggesting that land snails use HSPs as part of their survival strategy. As climatic variation was found to be associated with HSP expression, we tested whether adaptation of land snails to different habitats affects HSP expression in two closely related Sphincterochila snail species, a desert species Sphincterochila zonata and a Mediterranean-type species Sphincterochila cariosa. Our study suggests that Sphincterochila species use HSPs as part of their survival strategy following desiccation and heat stress, and as part of the natural annual cycle of activity and aestivation. Our studies also indicate that adaptation to different habitats results in the development of distinct strategies of HSP expression in response to stress, namely the reduced expression of HSPs in the desert-inhabiting species. We suggest that these different strategies reflect the difference in heat and aridity encountered in the natural habitats, and that the desert species S. zonata relies on mechanisms and adaptations other than HSP induction thus avoiding the fitness consequences of continuous HSP upregulation.
Assuntos
Ecossistema , Proteínas de Choque Térmico/metabolismo , Caramujos/fisiologia , Animais , Dessecação , Caramujos/metabolismo , TemperaturaRESUMO
In search for an effective oral treatment for diabetes, we examined the capacity of glucose tolerance factor (GTF) extracted from yeast and administered orally to reduce hyperglycaemia in rat models exhibiting insulin deficiency. The cellular effect of GTF on the insulin signalling pathway was investigated in vitro. GTF (oral bolus), insulin (intraperitoneal) or their combination was administered to streptozotocin-diabetic (STZ) or hyperglycaemic Cohen diabetic-sensitive (hyp-CDs) rats. Blood glucose (BG) and insulin levels were measured in the postprandial (PP) state and during an oral glucose tolerance test. Deoxy-glucose transport and insulin signal transduction were assessed in 3T3-L1 adipocytes and myoblasts incubated with the GTF. Low dose of insulin produced a 34 and 12·5 % reduction in the PP-BG levels of hyp-CDs and STZ rats, respectively. GTF induced a 33 and 17 % reduction in the PP-BG levels of hyp-CDs and STZ rats, respectively. When combined with insulin, a respective decrease (58 and 42 %) in BG levels was observed, suggesting a partially additive (hyp-CDs) or synergistic (STZ rats) effect of the GTF and insulin. GTF did not induce insulin secretion in hyp-CDs rats, yet it lowered their BG levels, proposing an effect on glucose clearance by peripheral tissues. GTF induced a dose-dependent increase in deoxy-glucose transport into myoblasts and fat cells similar to insulin, while the combined treatment resulted in augmented transport rate. GTF induced a dose- and time-dependent phosphorylation of insulin receptor substrate 1, Akt and mitogen-activated protein kinase independent of insulin receptor phosphorylation. GTF exerts remarkable insulin-mimetic and insulin-potentiating effects, both in vivo and in vitro. It produces an insulin-like effect by acting on cellular signals downstream of the insulin receptor. These results demonstrate a potential source for a novel oral medication for diabetes.
Assuntos
Aminoácidos/farmacologia , Cromo/farmacologia , Insulina/administração & dosagem , Mimetismo Molecular , Ácidos Nicotínicos/farmacologia , Leveduras/química , Administração Oral , Aminoácidos/administração & dosagem , Aminoácidos/isolamento & purificação , Animais , Glicemia/análise , Linhagem Celular , Cromo/administração & dosagem , Cromo/isolamento & purificação , Diabetes Mellitus Experimental/sangue , Diabetes Mellitus Experimental/tratamento farmacológico , Teste de Tolerância a Glucose , Técnicas In Vitro , Insulina/sangue , Masculino , Ácidos Nicotínicos/administração & dosagem , Ácidos Nicotínicos/isolamento & purificação , Fosforilação , RatosRESUMO
Land snails are subject to daily and seasonal variations in temperature and in water availability and use heat shock proteins (HSPs) as part of their survival strategy. We tested whether the reproductive cycle of land snails affects the endogenous levels of HSPs, and their involvement in the reproductive process. We examined HSP levels in the foot tissue of two Sphincterochila species, S. cariosa and S. zonata, before and after laying eggs, and analyzed the albumen gland (reproductive organ) of both species and eggs of S. cariosa for the presence and quantity of various HSPs. Our study shows reduction in the expression level of Hsp70 isoforms and Hsp90 in S. zonata foot and of Hsp74 in S. cariosa foot during the period preceding egg laying compared to the post-reproductive stage. Hsp70 isoforms and Hsp25 were highly expressed in both large albumen glands and in freshly laid eggs of S. cariosa, whereas large albumen glands of S. zonata expressed mainly Hsp70 isoforms. We conclude that a trade-off between survival and fertility is responsible for the expression level of HSPs in the foot tissue of Sphincterochila snails. Our study shows that HSPs are involved in the reproductive process. We propose that parental provision of HSPs may be part of a "be prepared" strategy of Sphincterochila snails, and that HSPs may play important roles in the survival strategy of land snails during the early life stages. Our observations also highlight the importance of the reproductive status in study of whole organisms, especially when assessing the HSP response to stress.
Assuntos
Proteínas de Choque Térmico/metabolismo , Caramujos/fisiologia , Animais , Nível de Alerta , Tamanho do Órgão , Especificidade de Órgãos , Óvulo/citologia , Óvulo/metabolismo , Reprodução , Caramujos/anatomia & histologia , Caramujos/metabolismoRESUMO
Land snails are subject to daily and seasonal variations in temperature and in water availability, and have evolved annual cycles of activity and aestivation as part of their survival strategy. We tested in the field whether adaptation to different habitats affects the endogenous levels of heat shock proteins (HSPs) in two closely related Sphincterochila snail species, a desiccation-resistant desert species, Sphincterochila zonata, and a Mediterranean-type, desiccation-sensitive species, S. cariosa. We examined HSP levels in various tissues of snails during aestivation and after resumption of activity. Our study shows that, during aestivation, S. cariosa had higher standing stocks of Hsp70 in the foot and the hepatopancreas, and of small HSPs (sHSPs) in all the examined tissues, whereas S. zonata had higher stocks of Hsp70 in the kidney and of Hsp90 in the kidney and in the hepatopancreas. Arousal induced a general upregulation of HSPs, except for Hsp90, the expression of which in the foot was higher during aestivation. We suggest that the stress protein machinery is upregulated during arousal in anticipation of possible oxidative stress ensuing from the accelerating metabolic rate and the exit from the deep hypometabolic state. Our findings support the concept that, in land snails, aestivation and activity represent two distinct physiological states, and suggest that land snails use HSPs as important components of the aestivation mechanism, and as part of their survival strategy during and after arousal. Our study also indicates that adaptation to different habitats results in the development of distinct strategies of HSP expression with likely consequences for the ecology and distribution of land snails.
Assuntos
Clima Desértico , Proteínas de Choque Térmico/metabolismo , Caramujos/metabolismo , Animais , Estivação/fisiologia , Proteínas de Choque Térmico HSP70/metabolismo , Proteínas de Choque Térmico HSP90/metabolismo , Proteínas de Choque Térmico Pequenas/metabolismo , Laboratórios , Região do Mediterrâneo , Especificidade de Órgãos , Concentração Osmolar , Estresse Oxidativo , Isoformas de Proteínas/metabolismo , Especificidade da Espécie , Análise de SobrevidaRESUMO
Land snails are subject to daily and seasonal variations in temperature and in water availability and depend on a range of behavioral and physiological adaptations for coping with problems of maintaining water, ionic, and thermal balance. Heat shock proteins (HSPs) are a multigene family of proteins whose expression is induced by a variety of stress agents. We used experimental desiccation to test whether adaptation to different habitats affects HSP expression in two closely related Sphincterochila snail species, a desiccation-resistant, desert species Sphincterochila zonata, and a Mediterranean-type, desiccation-sensitive species Sphincterochila cariosa. We examined the HSP response in the foot, hepatopancreas, and kidney tissues of snails exposed to normothermic desiccation. Our findings show variations in the HSP response in both timing and magnitude between the two species. The levels of endogenous Hsp72 in S. cariosa were higher in all the examined tissues, and the induction of Hsp72, Hsp74, and Hsp90 developed earlier than in S. zonata. In contrary, the induction of sHSPs (Hsp25 and Hsp30) was more pronounced in S. zonata compared to S. cariosa. Our results suggest that land snails use HSPs as part of their survival strategy during desiccation and as important components of the aestivation mechanism in the transition from activity to dormancy. Our study underscores the distinct strategy of HSP expression in response to desiccation, namely the delayed induction of Hsp70 and Hsp90 together with enhanced induction of sHSPs in the desert-dwelling species, and suggests that evolution in harsh environments will result in selection for reduced Hsp70 expression.
Assuntos
Proteínas de Choque Térmico/metabolismo , Caramujos/metabolismo , Animais , Dessecação , Proteínas de Choque Térmico HSP30/metabolismo , Proteínas de Choque Térmico HSP70/metabolismo , Proteínas de Choque Térmico HSP72/metabolismo , Proteínas de Choque Térmico HSP90/metabolismo , Proteínas de Choque Térmico Pequenas/metabolismo , Estresse FisiológicoRESUMO
Protective autoimmunity was only recently recognized as a mechanism for attenuating the progression of neurodegeneration. Using a rat model of optic nerve crush or contusive spinal cord injury, and a mouse model of neurodegenerative conditions caused by injection of a toxic dose of intraocular glutamate, we show that a single low dose of whole-body or lymphoid-organ gamma-irradiation significantly improved the spontaneous recovery. Animals with severe immune deficiency or deprived of mature T cells were unable to benefit from this treatment, suggesting that the irradiation-induced neuroprotection is immune mediated. This suggestion received further support from the findings that irradiation was accompanied by an increased incidence of activated T cells in the lymphoid organs and peripheral blood and an increase in mRNA encoding for the pro-inflammatory cytokines interleukin-12 and interferon-gamma, and that after irradiation, passive transfer of a subpopulation of suppressive T cells (naturally occurring regulatory CD4(+)CD25(+) T cells) wiped out the irradiation-induced protection. These results suggest that homeostasis-driven proliferation of T cells, induced by a single low-dose irradiation, leads to boosting of T cell-mediated neuroprotection and can be utilized clinically to fight off neurodegeneration and the threat of other diseases in which defense against toxic self-compounds is needed.
Assuntos
Raios gama/uso terapêutico , Homeostase/efeitos da radiação , Neurônios/efeitos da radiação , Linfócitos T/efeitos da radiação , Animais , Divisão Celular/imunologia , Divisão Celular/efeitos da radiação , Sobrevivência Celular/imunologia , Sobrevivência Celular/efeitos da radiação , Sistema Nervoso Central/imunologia , Sistema Nervoso Central/efeitos da radiação , Relação Dose-Resposta à Radiação , Feminino , Homeostase/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos SCID , Neurônios/citologia , Neurônios/imunologia , Traumatismos do Nervo Óptico/imunologia , Traumatismos do Nervo Óptico/terapia , Ratos , Ratos Endogâmicos Lew , Ratos Sprague-Dawley , Células Ganglionares da Retina/citologia , Células Ganglionares da Retina/imunologia , Células Ganglionares da Retina/efeitos da radiação , Linfócitos T/citologia , Linfócitos T/imunologiaRESUMO
PURPOSE: To investigate the antigenic specificity of the immune neuroprotective mechanism that can protect retinal ganglion cells (RGCs) against death caused by high intraocular pressure (IOP). METHODS: A unilateral increase in IOP was induced in rats by argon laser photocoagulation of the episcleral veins and limbal plexus. Rats with high IOP were immunized with glatiramer acetate (Cop-1, a synthetic copolymer) or with myelin-derived or uveitogenic peptides. When the steroid drug methylprednisolone was used, it was administered intraperitoneally every other day for 12 days. RESULTS: Vaccination with myelin-derived peptides that reside in the axons failed to protect RGCs from death caused by high IOP. In contrast, IOP-induced RGC loss was reduced by vaccination with R16, a peptide derived from interphotoreceptor retinoid-binding protein, an immunodominant antigen residing in the eye. The benefit of protection against IOP-induced RGC loss outweighed the cost of the monophasic experimental autoimmune uveitis (EAU) that transiently developed in a susceptible rat strain. Treatment with methylprednisolone alleviated the disease symptoms, but caused further loss of RGCs. Cop-1 vaccination was effective in both EAU-resistant and EAU-susceptible strains. CONCLUSIONS: To benefit damaged neurons, immune neuroprotection should be directed against immunodominant antigens that reside in the site of damage. In a rat model of high IOP, RGCs can benefit from vaccination with peptides derived from proteins that are immunodominant in the eye but not from myelin-associated proteins. This suggests that the site of primary degeneration in IOP-induced RGC loss is in the eye. Cop-1 vaccination apparently circumvents the site-specificity barrier and provides protection without risk of inducing autoimmune disease.
Assuntos
Proteínas do Olho , Glaucoma/prevenção & controle , Epitopos Imunodominantes/imunologia , Oligopeptídeos/imunologia , Proteínas de Ligação ao Retinol/imunologia , Vacinação , Vacinas Sintéticas/uso terapêutico , Animais , Doenças Autoimunes/induzido quimicamente , Doenças Autoimunes/tratamento farmacológico , Sobrevivência Celular/efeitos dos fármacos , Citoproteção , Modelos Animais de Doenças , Glaucoma/patologia , Pressão Intraocular , Masculino , Metilprednisolona/uso terapêutico , Proteína Básica da Mielina/imunologia , Hipertensão Ocular/prevenção & controle , Ratos , Ratos Endogâmicos Lew , Ratos Sprague-Dawley , Células Ganglionares da Retina/efeitos dos fármacos , Células Ganglionares da Retina/patologia , Uveíte/induzido quimicamente , Uveíte/tratamento farmacológico , Vacinas Sintéticas/efeitos adversosRESUMO
Glutamate, a key neurotransmitter, is pivotal to CNS function. Alterations in its concentration can be dangerous, as seen for example in acute injuries of the CNS, chronic neurodegenerative disorders and mental disorders. Its homeostasis is attributed to the efficient removal of glutamate from the extracellular milieu by reuptake via local transport mechanisms. Our recent studies suggest that glutamate, either directly or indirectly, elicits a purposeful systemic T-cell-mediated immune response directed against immunodominant self-antigens that reside at the site of glutamate-induced damage. We suggest that the harnessed autoimmunity (which we have termed 'protective autoimmunity') helps the resident microglia in their dual function as antigen-presenting cells (serving the immune system) and as cells that clear the damaged site of potentially harmful material (serving the nervous system). The interplay between glutamate and an adaptive immune response illustrates the bidirectional dialog between the immune and nervous systems, under both physiological and pathological conditions. These results point to the possible development of a therapeutic vaccination with self-antigens, or with antigens cross-reactive with self-antigens, as a way to augment autoimmunity without inducing an autoimmune disease, thus providing a safe method of limiting degeneration. This approach, which boosts a physiological mechanism for the regulation of glutamate, and possibly also that of other self-compounds, might prove to be a feasible strategy for therapeutic protection against glutamate-associated neurodegenerative or mental disorders.
Assuntos
Autoimunidade/imunologia , Sistema Nervoso Central/imunologia , Ácido Glutâmico/imunologia , Tolerância Imunológica/fisiologia , Linfócitos T/imunologia , Animais , Sistema Nervoso Central/lesões , Inflamação/imunologia , Camundongos , Degeneração Neural/imunologia , Doenças Neurodegenerativas/imunologia , Doenças Neurodegenerativas/prevenção & controle , Doenças Neurodegenerativas/terapia , Ratos , Vacinas/imunologia , Vacinas/uso terapêuticoRESUMO
Immune cells have been shown to contribute to spontaneous recovery from central nervous system (CNS) injury. Here we show that adult female rats and mice recover significantly better than their male littermates from incomplete spinal cord injury (ISCI). This sexual dimorphism is wiped out and recovery is worse in adult mice deprived of mature T cells. After spinal cord contusion in adult rats, functional recovery (measured by locomotor scores in an open field) was significantly worse in females treated with dihydrotestosterone prior to the injury than in placebo-treated controls, and significantly better in castrated males than in their noncastrated male littermates. Post-traumatic administration of the testosterone receptor antagonist flutamide promoted the functional recovery in adult male rats. These results, in line with the known inhibitory effect of testosterone on cell-mediated immunity, suggest that androgen-mediated immunosuppression plays a role in ISCI-related immune dysfunction and can therefore partly explain the worse outcome of ISCI in males than in female. We suggest that females, which are more prone to develop autoimmune response than males, benefit from this response in cases of CNS insults.
Assuntos
Autoimunidade , Recuperação de Função Fisiológica/imunologia , Traumatismos da Medula Espinal/imunologia , Antagonistas de Androgênios/farmacologia , Animais , Castração , Di-Hidrotestosterona/farmacologia , Feminino , Flutamida/farmacologia , Locomoção/efeitos dos fármacos , Locomoção/imunologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Ratos , Ratos Sprague-Dawley , Recuperação de Função Fisiológica/efeitos dos fármacos , Fatores Sexuais , Traumatismos da Medula Espinal/patologiaRESUMO
Vaccination with peptides derived from interphotoreceptor retinoid-binding protein (a self-Ag that can cause experimental autoimmune uveoretinitis) resulted in protection of retinal ganglion cells from glutamate-induced death or death as a consequence of optic nerve injury. In the case of glutamate insult, no such protection was obtained by vaccination with myelin Ags (self-Ags associated with an autoimmune disease in the brain and spinal cord that evokes a protective immune response against consequences of injury to myelinated axons). We suggest that protective autoimmunity is the body's defense mechanism against destructive self-compounds, and an autoimmune disease is the outcome of a failure to properly control such a response. Accordingly, the specific self-Ag (although not necessarily its particular epitopes) used by the body for protection against potentially harmful self-compounds (e.g., glutamate) can be inferred from the specificity of the autoimmune disease associated with the site at which the stress occurs (irrespectively of the type of stress) and is in need of help.
Assuntos
Autoantígenos/imunologia , Autoantígenos/uso terapêutico , Doenças Autoimunes/imunologia , Proteínas de Ligação ao Retinol/imunologia , Proteínas de Ligação ao Retinol/uso terapêutico , Uveíte/imunologia , Sequência de Aminoácidos , Animais , Autoantígenos/administração & dosagem , Doenças Autoimunes/patologia , Doenças Autoimunes/prevenção & controle , Bovinos , Proteínas do Olho/administração & dosagem , Proteínas do Olho/imunologia , Proteínas do Olho/uso terapêutico , Feminino , Ácido Glutâmico/toxicidade , Humanos , Epitopos Imunodominantes/administração & dosagem , Epitopos Imunodominantes/imunologia , Epitopos Imunodominantes/uso terapêutico , Masculino , Dados de Sequência Molecular , Compressão Nervosa , Nervo Óptico/imunologia , Nervo Óptico/patologia , Especificidade de Órgãos/imunologia , Fragmentos de Peptídeos/administração & dosagem , Fragmentos de Peptídeos/imunologia , Fragmentos de Peptídeos/uso terapêutico , Ratos , Ratos Endogâmicos F344 , Ratos Endogâmicos Lew , Ratos Sprague-Dawley , Células Ganglionares da Retina/efeitos dos fármacos , Células Ganglionares da Retina/imunologia , Células Ganglionares da Retina/patologia , Retinite/imunologia , Retinite/patologia , Retinite/prevenção & controle , Proteínas de Ligação ao Retinol/administração & dosagem , Solubilidade , Uveíte/patologia , Uveíte/prevenção & controle , VacinaçãoRESUMO
The ability of rats or mice to withstand the consequences of injury to myelinated axons in the CNS was previously shown to depend on the ability to manifest a T cell-mediated protective immune response, which is amenable to boosting by myelin-specific T cells. Here we show that this ability, assessed by retinal ganglion cell survival after optic nerve injury or locomotor activity after spinal cord contusion, is decreased if the animals were immunized as neonates with myelin proteins (resulting in their nonresponsiveness as adults to myelin proteins) or injected with naturally occurring regulatory CD4(+)CD25(+) T cells immediately after the injury, and is improved by elimination of these regulatory T cells. In nude BALBc mice replenished with a splenocyte population lacking CD4(+)CD25(+) regulatory T cells, significantly more neurons survived after optic nerve injury than in nude mice replenished with a complete splenocyte population or in matched wild-type controls. In contrast, neuronal survival in wild-type BALBc mice injected with CD4(+)CD25(+) regulatory T cells immediately after injury was significantly worse than in noninjected controls. These findings suggest that the ability to cope with the sequelae of a CNS insult is affected unfavorably by nonresponsiveness to myelin self-antigens and favorably by conditions allowing rapid expression of an autoimmune response. The regulatory T cells might represent an evolutionary compromise between the need to avoid autoimmune diseases and the need for autoimmunity on alert for the purpose of tissue maintenance.
Assuntos
Autoimunidade/fisiologia , Linfócitos T CD4-Positivos/imunologia , Sistema Nervoso Central/patologia , Neurônios/patologia , Traumatismos do Nervo Óptico/patologia , Células Ganglionares da Retina/patologia , Traumatismos da Medula Espinal/patologia , Subpopulações de Linfócitos T/imunologia , Transferência Adotiva , Animais , Axônios , Separação Celular , Sobrevivência Celular , Sistema Nervoso Central/imunologia , Suscetibilidade a Doenças , Feminino , Raios gama/efeitos adversos , Cobaias , Imunização , Depleção Linfocítica , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Proteína Básica da Mielina/imunologia , Compressão Nervosa , Regeneração Nervosa , Neurônios/imunologia , Traumatismos do Nervo Óptico/imunologia , Traumatismos do Nervo Óptico/terapia , Quimera por Radiação , Lesões Experimentais por Radiação/imunologia , Ratos , Ratos Endogâmicos Lew , Ratos Sprague-Dawley , Receptores de Interleucina-2/análise , Células Ganglionares da Retina/imunologia , Traumatismos da Medula Espinal/imunologia , Traumatismos da Medula Espinal/terapia , Subpopulações de Linfócitos T/transplante , TimectomiaRESUMO
Myelin-specific encephalitogenic T cells, when passively transferred into rats or mice, cause an experimental autoimmune disease. Previous studies by our group have shown that (a) the same cells also significantly reduce post-traumatic degeneration in these animals after injury to the central nervous system, (b) this beneficial autoimmunity is a physiological response, and (c) animals differ in their ability to resist injurious conditions, and the ability to resist post-traumatic degeneration correlates with resistance to the development of an autoimmune disease. Here we show that optic nerve neurons in both resistant and susceptible rat strains can be protected from secondary degeneration after crush injury by immunization with myelin basic protein emulsified in complete or incomplete Freund's adjuvant. We provide evidence that potentially destructive autoimmunity (causing autoimmune disease) and beneficial autoimmunity (causing improved neuronal survival) both result from activity of the same myelin-specific, proinflammatory Th1 cells. We further show that following passive transfer of such Th1 cells, the expression of their beneficial potential depends on the activity of an additional T cell (CD4(+)) population. By identifying the additional cellular component of autoimmune neuroprotection, we may be able to take meaningful steps toward achieving neuroprotection without risk of accompanying autoimmune disease.