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1.
World J Microbiol Biotechnol ; 28(5): 2125-37, 2012 May.
Artigo em Inglês | MEDLINE | ID: mdl-22806035

RESUMO

The present study aimed to isolate actinobacteria from soil samples and characterized them using molecular tools and screened their secondary metabolites for antimicrobial activities. Thirty-nine strains from four different location of Barrientos Island, Antarctica using 12 types of isolation media was isolated. The isolates were preceded to screening of secondary metabolites for antimicrobial and antifungal activities. Using high-throughput screening methods, 38% (15/39) of isolates produced bioactive metabolites. Approximately 18% (7/39), 18% (7/39), 10% (4/39) and 2.5% (1/39) of isolates inhibited growth of Candida albicans ATCC 10231(T), Staphylococcus aurues ATCC 51650(T), methicillin-resistant Staphylococcus aurues (MRSA) ATCC BAA-44(T) and Pseudomonas aeruginosa ATCC 10145(T), respectively. Molecular characterization techniques like 16S rRNA analysis, Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), Random amplified polymorphic DNA (RAPD) and composite analyses were used to characterize the actinobacteria strains. Analysis of 16S rRNA sequences is still one of the most powerful methods to determine higher taxonomic relationships of Actinobacteria. Both RAPD and ERIC-PCR fingerprinting have shown good discriminatory capability but RAPD proved to be better in discriminatory power than ERIC-PCR. Our results demonstrated that composite analysis of both fingerprinting generally increased the discrimination ability and generated best clustering for actinobacteria strains in this study.


Assuntos
Actinobacteria/isolamento & purificação , Actinobacteria/metabolismo , Anti-Infecciosos/metabolismo , Produtos Biológicos/metabolismo , Regiões Antárticas , Candida albicans/efeitos dos fármacos , Análise por Conglomerados , Impressões Digitais de DNA , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Avaliação Pré-Clínica de Medicamentos , Ensaios de Triagem em Larga Escala , Filogenia , Reação em Cadeia da Polimerase , Pseudomonas aeruginosa/efeitos dos fármacos , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Microbiologia do Solo , Staphylococcus/efeitos dos fármacos
2.
Malays J Pathol ; 33(1): 47-51, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21874752

RESUMO

Myeloid sarcoma (MS) is a rare extramedullary myeloid tumour. It has been reported in various sites, including lymph node, bone, skin, soft tissue, various organs and the CNS. It may precede or occur concurrently with acute myeloid leukemia. Urinary bladder involvement is extremely uncommon. We report a 70-year-old female who had MS of the urinary bladder, presented with frank and persistent hematuria associated with lower abdominal pain. She subsequently had tumour seeding in the abdominal skin via percutaneous suprapubic catheter. Tumours from both the urinary bladder and skin showed immature cells that were immunoreactive toward LCA (focal), MPO (strong), CD99 (weak) and CD117 (weak). Summary of cases in the literature is presented. The potential of its misdiagnosis and the useful markers for the diagnosis of MS are discussed.


Assuntos
Cateterismo/efeitos adversos , Inoculação de Neoplasia , Sarcoma Mieloide/patologia , Neoplasias Cutâneas/etiologia , Neoplasias da Bexiga Urinária/patologia , Idoso , Cistostomia , Feminino , Humanos , Neoplasias Cutâneas/patologia
3.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-630046

RESUMO

Myeloid sarcoma (MS) is a rare extramedullary myeloid tumour. It has been reported in various sites, including lymph node, bone, skin, soft tissue, various organs and the CNS. It may precede or occur concurrently with acute myeloid leukemia. Urinary bladder involvement is extremely uncommon. We report a 70-year-old female who had MS of the urinary bladder, presented with frank and persistent hematuria associated with lower abdominal pain. She subsequently had tumour seeding in the abdominal skin via percutaneous suprapubic catheter. Tumours from both the urinary bladder and skin showed immature cells that were immunoreactive toward LCA (focal), MPO (strong), CD99 (weak) and CD117 (weak). Summary of cases in the literature is presented. The potential of its misdiagnosis and the useful markers for the diagnosis of MS are discussed

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