Knowledge and Attitude of Undergraduate Students and Interns toward Stem Cells and Their Implications in Dentistry: A Cross-sectional Study.

AIM: The primary ideology of this research was to evaluate both the knowledge and attitude of undergraduate students and interns regarding stem cells and their use in dentistry. MATERIALS AND METHODS: A cross-sectional observatory study was conducted among 310 dental students of Jazan University by making use of a self-explanatory questionnaire, containing a set of 25 questions and was sent as e-mail through Google e-forms. RESULTS: There was a good response rate of around 81.2%, of which 80.16% were aware of the fact that stem cells are unspecialized types of cells that come with the potential to turn into any cell type. Almost 65.08% of the participants considered the possibility to continue root formation post-trauma as the fundamental use of stem cells, and a majority (71.43%) of the participants showed great interest in garnering more knowledge and training in the area of stem cells. About 50% of the participants showed strong opposition to the concept of embryonic stem cells as these are basically fetched from an aborted fetus or from an embryo. There was a major section of the participants (79.76%) who were even keen on organizing more programs to create public awareness regarding stem cells. Mean knowledge and attitude scores among the participants were reported to be 7.57 ± 3.10 and 36.04 ± 6.51 respectively. CONCLUSION: Overall, the level of knowledge and attitude of dental students and interns at Jazan University towards stem cells and their implications in dentistry can be interpreted as adequate. CLINICAL SIGNIFICANCE: Advanced training and awareness campaigns on stem cells and dentistry would improve their knowledge and attitude.

Recommendations for standard criteria for the positional and morphological evaluation of temporomandibular joint osseous structures using cone-beam CT: a systematic review.

OBJECTIVE: This systematic review aimed to appraise the reliability and comprehensiveness of imaging methods in studies that used three-dimensional assessment of the temporomandibular joint (TMJ) in order to propose a standardized imaging method. METHODS: Six databases/search engines were searched up until September 2022. The outcomes of interest included measurements of the mandibular condyle, glenoid fossa, joint spaces, or the entire TMJ. Two checklists were utilized: one to assess the risk of bias, with a maximum score of 37, and the other, a pre-designed checklist consisting of 22 items to evaluate the comprehensiveness of the methods used, with a maximum score of 33. RESULTS: Out of the 2567 records retrieved, only 14 studies, which used cone bean computed tomography (CBCT), were deemed eligible and thus included in the qualitative analysis. Three studies were deemed of low risk of bias, while the remaining studies were rated as moderate to high risk of bias, primarily due to improper reporting of inter-observer agreement, varying reliability values, and a limited number of cases included in the reliability analysis. Regarding the comprehensiveness of the methods used, only four studies achieved relatively high scores. The deficiencies observed were related to the reporting of variables such as slice thickness and voxel size, absence of or improper reporting of intra- and inter-examiner reliability analyses, and failure to assess all osseous components of the TMJ. CONCLUSION: CBCT-based methods used to assess the positions and morphology of TMJ bony structures appear to be imperfect and lacking in comprehensiveness. Hence, criteria for a standardized assessment method of these TMJ structures are proposed. CLINICAL RELEVANCE STATEMENT: Accurately, comprehensively, and reliably assessing the osseous structures of the temporomandibular joint will provide valid and valuable diagnostic features of the normal temporomandibular joint, and help establish potential associations between these osseous features and temporomandibular disorders. REGISTRATION: The protocol for this systematic review was registered at the International Prospective Register of Systematic Reviews (PROSPERO, No.: CRD42020199792). KEY POINTS: •Although many methods have been introduced to assess the osseous structure of the temporomandibular joint, they yielded inconsistent findings. •None of the published studies comprehensively assessed the temporomandibular joint. •Recommendations for a comprehensive temporomandibular joint osseous assessment method were suggested for better validity and reliability of future research.