Bovine muscle 20S proteasome. II: Contribution of the 20S proteasome to meat tenderization as revealed by an ultrastructural approach.

The role of the 20S proteasome proteolytic effects was revisited using an ultrastructural approach with the aim to explain some particular structural changes identified in type I muscles and in high pH meat. In both types of meat, major changes observed after ageing are an increase in the thickness of the Z-line followed by the appearance of an amorphous protein structure spreading out over the I-band. This was followed by a total degradation of this amorphous structure and of the Z-line. Partial transversal fragmentation of the myofibrils within the I-band can also be detected. The data reported clearly demonstrate that the 20S proteasome was able to mimic these sequential structural changes, a feature never obtained with either calpains or cathepsins. It is the first time that a direct implication of this complex in postmortem muscle is postulated.

Association of calpastatin (CAST/MspI) polymorphism with meat quality parameters of fatteners and its interaction with RYR1 genotypes.

The aim of this study was to analyse the effect of polymorphism of calpastatin (CAST) and RYR1 genes for some meat quality traits taking into consideration the group of meatiness and their mutual interactions. Investigations were carried out on a group of 201 fatteners which were crosses of four breeds. The results obtained show that CAST/MspI genotype has an effect close to the major effect for drip loss from LL muscle tissue at 48 h as well as at 96 h postmortem (0.92 and 0.96 SD respectively). Among the meat quality traits analysed and that were affected by CAST/MspI genotype, the animals with BB genotype at this locus were characterized by the most profitable values of all these traits. Significant interactions between CAST and RYR1 genotypes observed indicate that the quality of meat influenced by RYR1 genotype may be modified by the simultaneous influence of genotype as regards the CAST locus.

Lack of effects of hyperkalemia on the metabolism of normoxic or anoxic rabbit triceps brachii muscle.

Rabbit triceps brachii muscle was perfused with bovine red cells medium. Changes in phosphorus compounds and intracellular pH were followed using (31)P NMR during 15 min in the perfused muscle and during 50 min in muscle made anoxic by perfusion stop. Potassium levels in perfusate was maintained at 4 mM (normal plasma concentration at rest) during all perfusion in one muscle and at 4 mM for 10 min then 10 mM during 5 min before perfusion stop in the contralateral muscle. The intracellular pH and phosphorylated compounds content remained stable in the perfused muscle whatever the potassium concentration of the perfusate. Five min after perfusion stop a decrease in phosphocreatine (P<0.05) and pH (P<0.01) and an increase in sugar-phosphates (P<0.01) were observed independently of potassium concentration. The lack of effect of increasing circulating potassium indicates that hyperkalemia does not affect, by itself the muscles energetic metabolism.

Lipolysis and lipid oxidation during chilled storage of meat from Large White and Pietrain pigs.

The day after slaughter, six chops of Longissimus lumborum from each of 10 Large White pigs and six Piétrain pigs were packed individually under vacuum and kept at 3-4 °C in the dark. At 1, 5 and 9 days after slaughter, two chops were used for analysis of lipids, thiobarbituric acid reactive substances (TBARS), sarcoplasmic Ca(2+) and sarcoplasm and mitochondria phospholipase A(2) activity. Free fatty acid content was higher in Piétrains than in Large Whites and increased with keeping time. Total lipids of Large White pigs contained more saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA) and less polyunsaturated fatty acid (PUFA). Large Whites had less dioleoyl-linoleyl-glycerol (OOL) and Palmitoyl-oleoyl-linoleyl-glycerol (POL) and more Palmitoyl-oleoyl-stearyl-glycerol (POS) than Piétrains. The percentages of SFA and MUFA decreased and the percentage of PUFA increased with time in FFA of Large White pigs. In Piétrains, similar changes were observed between days 1 and 5, but later the percentages of SFA increased and the percentage of PUFA decreased again. TBARS tended to increase with time particularly in Piétrains. Sarcoplasmic phospholipase A(2) decreased between days 5 and 9 in both breeds. Sarcoplasmic calcium was markedly higher at day 1 in Piétrains than in Large Whites then the difference decreased. These breed differences in lipid composition differences and lipid changes during storage are considered too small to be of practical importance, for instance in influencing the choice of a breed for pork production.

Effect of stunning conditions on occurence of PSE defects in hams of rn+/RN(-) pigs.

Pigs of the same genetic type at the RN and HAL loci, i.e. rn(+)RN(-)/NN were reared in similar conditions of feeding and housing. They were slaughtered in two abattoirs (referred to as A1 and A2) using a mixture of air (30%) and CO(2) (70%), at a rate of 300 pigs per hour per slaughterline. One hundred and thirty-two pigs from 11 farms were slaughtered in A1 using a corusinga restrainer and 127 pigs from 5 farms were slaughtered in A2 with the backloading technique. pH at 40 min, 2.5 h and 24 h after slaughter and colour (L*, a*, b*) at 24 h after slaughter were measured in the semimembranosus muscle. Meat quality of the ham was scored as follows: 1, no PSE-zone; 2, doubtful; 3, PSE-zones in the semimembranosus and sometimes on the internal flexor muscles; 4, PSE-zones in all the flexor muscles. The muscle pH value was higher in A2 than in A1 at 40 min post mortem (P<0.01), but not at 2.5 and 24 h. L* (P<0.001) and b* (P<0.05) were higher in A1 than in A2. There was a remarkable difference in meat quality scores, with 50% of the hams scoring 3 or 4 in A1, vs 13% in A2. Lairage time before slaughter affected (P<0.01) the pH value at 2.5 h (5.69 vs 5.93). The values of pH1 and pH2.5 decreased with increasing the meat quality score. The values of L* and b* increased markedly with the score. The results of the present study indicate that the method of bringing the slaughter pigs to the stunning device affects the frequency and importance of PSE meat in the ham. The automated driving of groups of animals to the stunning machine combined with the backloading of a nacelle, compared to a traditional system driving pigs in single file using electrical goads and a restrainer was beneficial with respect to both meat quality and animal welfare.

Pork quality, processing, and sensory characteristics of dry-cured hams as influenced by Duroc crossing and sex.

This study was designed to evaluate Duroc (DU) crossing for Carso dry-cured ham production. One hundred fifty-four pigs (81 females and 73 castrates) of four different genotypes, pure Landrace pigs (LAN), offspring of LAN females crossed with Large White (LW) males (LWxLAN), offspring of LAN females crossed with DU males (DUxLAN), and offspring of LWxLAN females crossed with DU males (DUx[LWxLAN]), were chosen in the weight range of 105 to 120 kg (112.7 +/- 0.4 kg). Raw material quality was evaluated for ham fatness (intra- and intermuscular and subcutaneous) and meat quality (pH, color, water-holding capacity) of longissimus dorsi, biceps femoris, and semimembranosus muscles. Ham weight losses were recorded at different stages of processing. The biceps femoris and semimembranosus muscles were analyzed for chemical composition before (lipid, moisture, total nitrogen, nonprotein nitrogen) and after (moisture, salt, total nitrogen, nonprotein nitrogen) processing. Chemical and sensory analyses were performed on 96 dry hams (12 castrates and 12 females per genotype). Biceps femoris and semimembranosus muscles were evaluated for color, saltiness, aroma, and texture. Pigs of the four genotypes had similar ham fatness as estimated by subcutaneous fat thickness. Duroc crosses exhibited higher intramuscular fat content, marbling, and intermuscular fat. Crossing with DU resulted in lower weight losses during ham processing. Castrates were fatter and had more intra- and intermuscular fat and lower ham processing weight losses than females. A strong negative relationship between ham fatness and ham processing losses was observed. Chemical and sensory traits of dry ham muscles were little affected by DU crossing. Lower salt content of biceps femoris was found in DU crosses. Dry hams from female pigs had higher total and nonprotein nitrogen, but drier, firmer texture and higher resistance to cutting force compared to dry hams from castrated pigs. Crossing with DU demonstrated some disadvantages (more intermuscular fat, more slice visible fat) and advantages (lower weight loss and salt intake) for the quality of dry-cured ham.

Temperature and catecholamine effects on metabolism of perfused isolated rabbit muscle.

Biceps brachii muscle was isolated from anaesthetized New Zealand rabbits and perfused for 30 min with bovine red cells, while electrically stimulated at 0.1 Hz, 50 V. Temperature was maintained at 34 (physiological temperature of this muscle), 36 or 38 °C in the first experiment (Exp 1). In the second experiment (Exp 2), temperature was maintained at either 34 or 38 °C and the muscle was perfused with 2 ng/ml adrenaline and 10 ng/ml noradrenaline for 5 min during the perfusion. Whatever the temperature, twitch force decreased (P<0.01) during perfusion indicating muscular fatigue. Total turnover rate of "energy-rich" phosphate was higher at 38 °C than at 34 °C (P<0.05) during the first 30 min after the perfusion was stopped in Exp 1 and tended to be higher between 30 and 60 min (P<0.10) in Exp 2. Catecholamine perfusion had no effect on contraction and on muscle metabolism whatever the temperature. Our results show that an increase in temperature of 4 °C above the physiological temperature decreases the resistance of oxygenated rabbit muscle to fatigue. In anoxic muscle, temperature increase accelerates the turnover of "energy-rich" phosphate compounds, which is known to be detrimental to meat quality.

Influence of intramuscular fat content on lipid composition, sensory qualities and consumer acceptability of cured cooked ham.

The present study is part of a project which aims to examine the influence of intramuscular fat (IMF) content on the sensory attributes and consumer acceptability of pork. An experiment was conducted to evaluate the influence of IMF level in muscle semimembranosus (SM) on the composition of its lipid fraction and on the sensory qualities and consumer acceptability of cured cooked hams. Thirty-two carcasses were selected 24 h after slaughter from 125 Duroc × Landrace castrated male pigs showing large variability in SM muscle IMF content and were assigned to four IMF groups: ≤2%, 2-3%, 3-4% and >4%. Cured cooked ham slices were evaluated by a trained expert panel of 12 members and by a group of 56 consumers. Results from lipid analyses indicate that (i) an increase in IMF content was almost entirely reflected by an increase in the triglyceride content of the muscle and (ii) higher IMF levels were associated with higher free fatty acid and monoglyceride and lower cholesterol levels. The sensory evaluation of marbling significantly increased with IMF level, whereas other sensory qualities were unaffected. High IMF levels significantly depreciated the consumer perception of fat, aspect, taste and smell of ham slices. Overall, slices with the highest IMF levels got the least favourable rating by consumers. The present study indicates that increased IMF levels in muscle semimembranosus do not have significant effects on the sensory attributes of cured cooked hams, apart from the perception of marbling. However, high IMF levels have detrimental effects on the acceptability by consumers. © 2000 Society of Chemical Industry.

Comparison between the three porcine RN genotypes for growth, carcass composition and meat quality traits.

A three-step experimental design has been carried out to add evidence about the existence of the RN gene, with two segregating alleles RN- and rn+, having major effects on meat quality in pigs, to estimate its effects on production traits and to map the RN locus. In the present article, the experimental population and sampling procedures are described and discussed, and effects of the three RN genotypes on growth and carcass traits are presented. The RN genotype had no major effect on growth performance and killing out percentage. Variables pertaining to carcass tissue composition showed that the RN- allele is associated with leaner carcasses (about 1 s.d. effect without dominance for back fat thickness, 0.5 s.d. effect with dominance for weights of joints). Muscle glycolytic potential (GP) was considerably higher in RN- carriers, with a maximum of a 6.85 s.d. effect for the live longissimus muscle GP. Physico-chemical characteristics of meat were also influenced by the RN genotype in a dominant way, ultimate pH differing by about 2 s.d. between homozygous genotypes and meat colour by about 1 s.d. Technological quality was also affected, with a 1 s.d. decrease in technological yield for RN- carriers. The RN genotype had a more limited effect on eating quality. On the whole, the identity between the acid meat condition and the RN- allele effect is clearly demonstrated (higher muscle GP, lower ultimate pH, paler meat and lower protein content), and the unfavourable relationship between GP and carcass lean to fat ratio is confirmed.

Influence of the three RN genotypes on chemical composition, enzyme activities, and myofiber characteristics of porcine skeletal muscle.

An experiment was conducted to evaluate the effects of the RN genotype on skeletal muscle characteristics in pigs sharing otherwise the same polygenic background. Animals were genotyped for RN on the basis of RTN (Rendement Technologique Napole) records using segregation analysis methods. Samples of longissimus (L) and semispinalis capitis (S) muscles were taken from 39 rn+/rn+, 38 RN-/rn+ and 37 RN-/RN- pigs slaughtered at 108 +/- 8.6 kg live weight. Activities of lactate dehydrogenase (LDH), citrate synthase (CS), and beta-hydroxy-acyl-coenzyme A dehydrogenase (HAD) were measured on both muscles to assess glycolytic, oxidative, and lipid beta-oxidation capacities, respectively. Histological examinations and chemical analyses were performed on L muscle. The energetic metabolism of the white L muscle was more oxidative in RN-/RN- than in rn+/rn+ pigs, as shown by increased CS and HAD activities (P < .001), decreased LDH activity (P < .001), larger cross-sectional area of IIA (P < .05) and IIB-red (P < .05) fibers, higher relative area of IIA fibers ( P < .05), and lower relative area of IIB-white fibers (P < .001). No significant difference was found between heterozygous and homozygous carriers of the RN- allele, except for CS activity, which was lower in RN-/rn+ than in RN-/RN- pigs. In L muscle, the RN- allele led to a large increase in glycolytic potential (+3.5 phenotypic SD between homozygotes) and lightness (+.7 SD), and a decrease in ultimate pH, dry matter, and protein contents (-1.7 to -2 phenotypic SD for these three traits), with an almost completely dominant effect. No differences were found between genotypes for intramuscular fat and hydroxyproline contents. In the red S muscle, the presence of RN- had no influence on enzyme activities. These results indicate that the RN genotype greatly influences compositional and histochemical traits and metabolic enzyme activities in a muscle type-dependent manner, with a completely or incompletely dominant effect of the RN- allele.

Effects of the halothane genotype and slaughter weight on texture of pork.

The objective of this study was to investigate the effects of the halothane (HAL) genotype, slaughter weight (SW), and the HAL x SW interaction on compositional and textural traits of raw and cooked pork. Pigs were bred to exhibit one of the three HAL genotypes (NN, Nn, and nn) with otherwise equivalent genomes. The nn halothane reactors are known to typically produce PSE pork, whereas NN pigs do not typically produce PSE pork. Pietrain x Large White gilts and boars, all with verified Nn genotype (by DNA test), were mated to obtain F2 littermates of the three HAL genotypes. These pigs were slaughtered at either 101 +/- 3 ("light") or 127 +/- 3 ("heavy") kg BW and were evaluated for longissimus muscle traits. The pH at .5 h after death (pH1) was 6.35, 6.13, and 5.68 in NN, Nn, and nn pigs, respectively. Sarcomere length was greater in nn than in NN and Nn pigs (1.94 vs 1.83 and 1.85 microm, respectively). Mechanical resistance was higher in nn than in NN pigs for both raw and cooked meat. Meat from nn pigs was judged by a trained panel to be less rough, more cohesive, harder, more fibrous, less granular, more elastic, and less easy to swallow than meat from NN pigs. For most traits under study, the heterozygotes were intermediate between the homozygotes but closer to NN than to nn pigs. Muscle from heavy pigs had longer sarcomeres and less moisture than muscle from light pigs. The n allele of the HAL gene unfavorably affects pork texture, and this effect is maintained throughout the range of 101 to 127 kg BW.

Influence of intramuscular fat content on the quality of pig meat - 1. Composition of the lipid fraction and sensory characteristics of m. longissimus lumborum.

The present study is part of a project which aimed to examine the influence of intramuscular fat (IMF) content on the sensory attributes and consumer acceptability of pork. Two experiments were conducted to evaluate the influence of IMF level on the composition of the lipid fraction and on the sensory qualities of muscle longissimus lumborum (LL). Each of these experiments used 32 castrated male pigs selected after slaughter either from 125 Duroc×Landrace (Experiment 1) or 102 Tia Meslan×Landrace (Experiment 2) crossbred animals, and showing large variability in LL IMF content: from <1.5 to >3.5% in Experiment 1 and from 1.25 to 3.25% in Experiment 2. Results from lipid analyses indicate that in both experiments, an increase in IMF content is almost entirely reflected by an increase in the triglycerides content of the muscle. In Experiment 2, higher IMF content was associated with higher free fatty acids. Marbling score was significantly affected by IMF level in Experiment 1 but not in Experiment 2. In Experiment 1, a trend towards a favourable effect of high IMF levels on flavour (p=0.09) and tenderness (p=0.055) was observed. In experiment 2, increased IMF level was associated with significantly higher juiciness and flavour scores. The results from the present study indicate that the variability in IMF level of LL muscle was almost entirely due to the variability in triglyceride contents. Favourable effects of increased IMF levels on the sensory attributes of pork were demonstrated in both experiments using different types of animals, but the nature and the magnitude of these effects depended on the experiment considered.

Influence of intramuscular fat content on the quality of pig meat - 2. Consumer acceptability of m. longissimus lumborum.

The present study is part of a project which aimed to examine the influence of intramuscular fat (IMF) content on sensory attributes and consumer acceptability of pork. Two experiments were conducted to evaluate consumer acceptability of pork chops with varying IMF level in muscle Longissimus lumborum (LL). Each experiment used 32 castrated male pigs selected after slaughter either from 125 Duroc × Landrace (Experiment 1) or 102 Tia Meslan × Landrace (Experiment 2) crossbred animals, and showing large variability in LL IMF content: from <1.5 to >3.5% in Experiment 1 and from 1.25 to 3.25% in Experiment 2. A group of 56 consumers evaluated various items on rib-eye (LL muscle trimmed of backfat) (Experiment 1) and on entire chops trimmed of backfat (Experiment 2). Data from Experiment 1 indicate that an increase in IMF level is associated with an increase in visual perception of fat and a corresponding decrease in the willingness to eat and purchase the meat, when expressed before tasting. The latter effect disappeared after the consumers had tasted the meat, probably due to a positive effect of increase IMF, up to 3.5%, on the perception of texture and taste. In Experiment 2, where entire chops were evaluated, the perception of visible fat was not affected by IMF level, probably due to the lack of variation in the level of intermuscular fat between the four IMF groups. The willingness to eat and purchase the meat were unaffected by IMF level, whereas the perception of texture and taste was enhanced with increased IMF levels up to 3.25%. The present data suggest that the acceptability of pork may be improved by increasing IMF level but: (1) this effect disappeared for IMF levels higher than 3.5%, which are associated with a high risk of meat rejection due to visible fat and (2) the positive effect of increased IMF probably holds true as long as it is not associated with an increase in the level of intermuscular fat.

Recent methods for predicting quality of whole meat.

The world of meat faces a permanent need for new methods of meat quality evaluation. Researchers want improved techniques to deepen their understanding of meat features. Expectations of consumers for meat quality grow constantly, which induces the necessity of quality control at the levels of slaughtering, meat cutting, and distribution. This article is focused on techniques intended to predict technological and sensory qualities from measurements carried out on fresh intact meat. pH has been measured for a long time, but its on-line determination still progresses through automation. In the laboratory, NMR provides new insights on WHC mechanisms. Image processing has considerably improved the assessment of meat appearance. Developments of techniques for prediction of toughness are in progress, either directly, through ultrasonic analysis or NIR reflectance, or indirectly, through determination of connective tissue content by fluorescence probes. Control of authenticity benefits from the last developments of molecular biology and analytical chemistry. However, implementation of methods for meat quality evaluation has been very limited in the industry. The reasons for that situation are analyzed. Among the techniques recently described, the most promising for large-scale meat quality evaluation are considered to be ultrasonic analysis, image processing and NIR spectroscopy.

Metabolic events with spontaneous malignant hyperthermia crisis in an anaesthetized pig.

PURPOSE: To analyze metabolic changes associated with a fulminant malignant hyperthermia (MH) crisis developed spontaneously in an MH susceptible pig which was part of 12 pigs undergoing metabolic investigation (six MH susceptible and six controls) and had been anaesthetized with a non-triggering agent (pentobarbitone). METHODS: The pig was placed in a cradle and then inserted into a 4.7 T magnet bore. The semi-membranous muscle was submitted to three repetitive stimulation-recovery sessions. 31-P magnetic resonance spectra and mechanical data were recorded. RESULTS: The pig developed a non-rigid MH crisis during recovery from the second set of experiments. Although no mechanical work was performed, dramatic metabolic changes were noted. Twitch tension decreased progressively reaching zero while mouth temperature continuously increased to 44.5 degrees C. Phosphocreatine (PCr) consumption was coupled to Pi accumulation. Also, a marked intracellular acidosis and a large accumulation of phosphomonoesters (PME) were observed, probably as a result of massive glycolysis activation. Interestingly, ATP level remained constant. CONCLUSION: These irreversible mechanisms may constitute a metabolic dead-end coupling calcium pumping ATP-consuming processes and ATP synthesis through PCr breakdown and anaerobic glycolysis. They do not differ from metabolic changes previously reported in rigid forms of MH crisis.

Phenotypic and genetic parameters for longissimus muscle fiber characteristics in relation to growth, carcass, and meat quality traits in large white pigs.

A total of 383 barrows and gilts from a French Large White experimental herd were slaughtered at 100 kg BW. Samples of longissimus muscle were taken to categorize myofibers according to their contractile (I, IIA, and IIB) and metabolic (oxidative and nonoxidative) properties. Myofiber percentages, cross-sectional areas (CSA), and relative areas were measured. Growth rate, carcass composition, muscle chemical composition, metabolic enzyme activities, and meat quality traits were also measured to estimate phenotypic and genetic correlations between these traits and myofiber characteristics. Genetic parameters were estimated using a REML procedure applied to an individual animal model. Heritabilities of fiber traits were moderate to high (h2 = .20 to .59). Highest heritabilities were found for type I fiber percentage (h2 = .46 +/- .11), type IIBw fiber percentage (h2 = .58 +/- .11), and type I fiber cross-sectional area (h2 = .59 +/- .10). For a given fiber type, the relative area was phenotypically and genetically more closely related to the percentage than to the CSA. Phenotypic correlations between fiber type composition and other traits were low. Genetically, growth rate, carcass leanness, and loin eye area were positively related to fiber CSA. Intramuscular fat content was not related to fiber type composition (r(g) = -.05 to .06), whereas it was positively related to fiber CSA (r(g) = .68). Type IIBw fiber percentage was related to pH at 30 min (r(g) = -.46), pH at 24 h (r(g) = -.62), glycolytic potential (r(g) = .31), and lightness of color (r(g) = .55) of longissimus muscle.

Chemical and structural changes in dry-cured hams (Bayonne hams) during processing and effects of the dehairing technique.

Pigs of similar genetic backgrounds and feeding regimes were slaughtered in two abattoirs, one carrying out dehairing by scalding and the other by singeing. One ham from each of 80 carcasses was retained. Sixteen fresh hams (8 from each dehairing technique) were used for analysis while 64 hams were processed into dry-cured ham. Sixteen hams (8 from each dehairing technique) were taken for analysis at end of salting (day 14), end of rest (day 78), mid-processing (day 127) and end of processing (day 251). During processing, the water content of all muscles decreased while the salt content increased. The salt concentration in muscle water tended to equalize in all muscles. The nitrogen content of desalted dry matter (i.e. dry muscle tissue) decreased in both Biceps femoris and Semimembranosus. The content of every free amino acid increased with time, except for taurine and glutamine. Electrophoresis of the low ionic strength-soluble fractions showed all protein bands decreased during processing. Electrophoresis of the myofibrillar fractions indicated changes in all bands except actin (42kDa). These changes were more marked in the Semimembranosus than the Biceps femoris in the earlier processing steps. Ultrastructural changes were more marked in Semimembranosus than Biceps femoris. Hardness and chewiness increased in both muscles during the first half of processing then returned to values close to the initial ones in Semimembranosus but changed little in Biceps femoris. The scalded hams lost more weight than the singed ones during processing. The salt content was higher in scalded hams. Water-soluble nitrogen and NPN were higher in singed hams at the end of processing. The scalded hams were saltier and pungent. They had more pronounced aromas of dry ham, rancidity and hazelnut, and less aroma of fresh meat. Their texture was drier and less mellow.

Effect of duration of feed withdrawal and transportation time on muscle characteristics and quality in Friesian-Holstein calves.

Twenty-week-old Friesian-Holstein calves were used to assess the influences of the duration of feed withdrawal before transport (1 or 11 h) and of transport time (1 or 11 h) on carcass and muscle characteristics and meat sensory qualities. One hundred twelve calves were used for live weight and carcass measurements, following a 2 x 2 factorial design (28 replicates). Twelve calves were randomly selected in each treatment group to examine muscle characteristics and sensory quality of longissimus lumborum (LL) and semimembranosus (SM). Long transport (11 h) increased loss in live weight (P < .001) and dressing percentage (P < .001). Feed withdrawal for 11 h also increased dressing percentage (P < .001). Long transport resulted in decreased liver weight (P < .05), glycolytic potential (P < .01), and pH at 4 h postmortem (P < .05) in the LL. Drip loss, compositional traits, cooking loss, and sarcomere length in the LL, as well as drip loss, pH values, compositional traits, cooking loss, and sarcomere length in the SM, were unaffected by the treatments. However, long transport decreased tenderness score in the LL (P < .05) and SM (P < .01). It also increased myofibrillar resistance (P < .001) in the latter muscle. The results demonstrate an unfavorable effect of long transport on the sensory quality of veal. This effect cannot be explained on the basis of differences in pH values and(or) compositional characteristics.

Effectson carcass weight and meat quality of pigs dehaired by scalding or singering post-mortem.

Two experiments were designed to compare the effects of two pig dehairing techniques. In the first experiment, 120 pigs were slaughtered in the same abattoir in five weekly sereis. One half of each series was dehaired by singeing (the usual technique in this abattoir), while the other half was dehaired in a scalding-dehairing combination. In the second experiment, 48 pigs were slaughtered in two series, one-half of each series in an abattoir practising singeing, the other half in an abattoir practising scalding. Temperature and pH were measured 30 min after slaughter in the longissimus lumborum. Meat quality was assessed the day after slaughter in various muscles. One ham was cured and cooked, while the other one was processed by dry-curing. In both experiments, carcass weights and yields were noticeably higher after singeing. Hams were heavier in singed carcasses, but their proportions in the carcasses were little or not at all affected by the dehairing technique. Proportions of loins, shoulders and bellies were similar with both dehairing techniques. The dehairing technique affected neither temperature nor rate of post-mortem pH fall. Meat quality as assessed on fresh meat was slightly better in singed carcasses. Weights of cooked ham and dry ham per side were higher in singed carcasses, due to the higher initial ham weight, but processing yields were not affected by the dehairing technique.