RESUMO
A potyvirus was found causing yellow mosaic and veinal banding in sweetpepper in Central and Southeast Brazil. The sequence analysis of the 3' terminal region of the viral RNA revealed a coat protein of 278 amino acids, followed by 275 nucleotides in the 3'-untranslated region preceding a polyadenylated tail. The virus shared 77.4% coat protein amino acid identity with Pepper severe mosaic virus, the closest Potyvirus species. The 3'-untranslated region was highly divergent from other potyviruses. Based on these results, the virus found in sweetpepper plants could be considered as a new potyvirus. The name Pepper yellow mosaic virus (PepYMV) is suggested.
Assuntos
Capsicum/virologia , Genoma Viral , Doenças das Plantas/virologia , Potyvirus/classificação , Regiões 3' não Traduzidas/genética , Sequência de Aminoácidos , Brasil , Capsídeo/genética , Clonagem Molecular , Dados de Sequência Molecular , Filogenia , Potyvirus/genética , Alinhamento de Sequência , Homologia de Sequência de AminoácidosRESUMO
Isolated submandibular suppurative sialadenitis in the newborn is a rare entity. Only six cases have been reported in the literature. A rare case of isolated bilateral submandibular abscesses in a premature infant is presented. The possible etiology, diagnosis and management of this rare condition are discussed.
Assuntos
Doenças em Gêmeos , Doenças do Prematuro/epidemiologia , Sialadenite/epidemiologia , Infecções Estafilocócicas/epidemiologia , Doenças da Glândula Submandibular/epidemiologia , Abscesso/epidemiologia , Abscesso/microbiologia , Feminino , Humanos , Recém-NascidoRESUMO
Protoplasts from different Daucus carota L. cell strains carrying resistance to glyphosate, 5-methyltryptophan, sodium selenate or selenocystine were fused in three combinations using dextran. Clones were selected for both of the resistances carried by the individual parental strains in medium with both inhibitors. No doubly resistant colonies formed from unfused controls or from protoplasts from each individual parental strain alone. Suspension cultures from the selected clones contained predominantly the additive chromosome numbers of the parental strains. Apparently the four resistances used are expressed dominantly in fusion hybrids. Analysis of mitochondrial DNA showed that recombination occurred in one fusion combination since the mitochondrial DNA in the hybrid cells was different from that of either parent as shown by restriction endonuclease fragment analysis. Mitochondrial DNA in the other two somatic hybrid combinations was parental. Thus, a dominant, nuclear resistance marker system has been developed to select efficiently for somatic hybrids in which mitochondrial DNA recombination can be studied.
