Targeted transcriptomic analysis of pancreatic adenocarcinoma in EUS-FNA samples by NanoString technology.

Background: Integration of transcriptomic testing into EUS-FNA samples is a growing need for precision oncology in pancreatic ductal adenocarcinoma (PDAC). The NanoString platform is suitable for transcriptome profiling in low yield RNA samples. Methods: Inclusion of patients that underwent EUS-FNA cytological diagnosis of pancreatic ductal adenocarcinoma using 19G and/or 22G needles and subsequent surgical resection. Formalin-fixed, paraffin-embedded (FFPE) cytological and surgical samples underwent RNA extraction and transcriptomic analysis using a custom 52-gene NanoString panel of stromal PDAC features. Cell type abundance was quantified in FFPE specimens and correlated. Results: 18 PDAC patients were included. Mean EUS-FNA passes was 2 + 0.7. All FFPE passed the RNA quality control for genomic analysis. Hierarchical clustering on the global gene expression data showed that genes were differentially expressed between EUS and surgical samples. A more enriched cancer-associated fibroblasts and epithelial-mesenchymal transition transcriptomic profile was observed across surgical specimens whereas immunological biomarkers were more represented in EUS-FNA samples. Cytological examination confirmed a scanty representation of CAF and more immunological cell abundance in cytological samples in comparison to surgical specimens. Conclusion: Targeted transcriptomic NanoString profiling of PDAC samples obtained by EUS-FNA is a feasible approach for pre-surgical molecular analysis although stromal CAF/EMT mRNA biomarkers are underrepresented.

[Effectiveness of a rehabilitation and occupational behavioral cognitive group intervention for chronic low back pain with low disability. Prospective study with a 6-month follow-up]. / Efficacia di un intervento riabilitativo ed occupazionale di gruppo ad impronta cognitive comportamentale per la lombalgia cronica a bassa disabilità. Studio prospettico, con follow-up a sei mesi.

BACKGROUND: Literature suggests a bio-psychosocial approach to chronic low back pain, heralding disability. A multidisciplinary combination of motor, occupational and cognitive behavioural therapies constitutes a rehabilitative approach provided with growing evidence in the clinic field of spinal chronic pain. OBJECTIVES: To evaluate the effectiveness of a motor, occupational and cognitive behavioural approach, carried out in group, for subjects with low disability chronic low back pain. METHODS: Prospective trial, with a six-month follow-up. We selected patients with chronic low back pain considered at low disability (Roland Morris Disability Questionnaire, RMDQ, score lower than 12/24), in the absence of serious co-morbidities, consecutively admitted to a Physical Medicine and Rehabilitation Unit. The patients underwent ten sessions of a rehabilitative and cognitive behavioural treatment, made in group. We identified the following outcome measures, administered pre-treatment, posttreatment and during six-month follow-up: pain (VAS), disability (RMDQ), health status (SF-36). We performed a parametric analysis for repeated measures (Student t test, significance: p < 0.05); we also searched the clinical importance for pain and disability. POPULATION: 37 subjects, 21 females, 16 males, mean age 52 +/- 11 years old, low back pain mean duration 10 +/- 4 months. The described approach was successful (VAS p = 0.001, RMDQ p = 0.001, SF-36 domains 0.001 < p < 0.014), with persistence of results at the six-month follow-up for Physical Role, Pain, Emotional Role and Mental Health SF-36 domains; further significance increase was achieved for Physical Activities (p = 0.009), General Health (p = 0.006), and Vitality SF-36 domains (p = 0.007). The Social Activities domain of the SF-36 questionnaire was not significant (p = 0.260) at the end of the trial, getting significance at the six-month follow-up (p = 0.001). Clinical significance was furthermore achieved for pain and disability outcome measures, unchanging six months later. CONCLUSIONS: The trial showed evidence in favour of a combined motor, occupational and cognitive-behavioural approach, carried out in group, for low disability chronic low back pain. Considering methodological biases of the study, we recommend confirmation of these results through randomized controlled trial.

[Muscular enzyme level disorders and a malignant hyperthermia susceptability test: postoperative study of a patient with elevated CPK]. / Valori costantemente alterati degli enzimi muscolari e test di suscettibilità all'ipertermia maligna: studio preoperatorio di un paziente con CPK elevato.

The preoperative evaluation of the enzymatic level concerning the functioning of the neuromuscular system, is of particular importance. The diseases of these regions may lead to Malignant Hyperthermia (MH) in the course of general anaesthesia. The authors illustrate the case of a young asymptomatic patient with constantly high levels of CPK, who was later affected by Central Core Disease (CCD), a rare hereditary slow progressing myopathy strictly related to MH. The authors suggest tat preoperative CPK levels, though not predictive of a patient to develop MH, can allow identification of patients affected by clinically silent myopathies. This can also acquire legal importance in case of clinical MH manifestation.