P-FLUX: A phosphorus budget dataset spanning diverse agricultural production systems in the United States and Canada.

Quantifying spatial and temporal fluxes of phosphorus (P) within and among agricultural production systems is critical for sustaining agricultural production while minimizing environmental impacts. To better understand P fluxes in agricultural landscapes, P-FLUX, a detailed and harmonized dataset of P inputs, outputs, and budgets, as well as estimated uncertainties for each P flux and budget, was developed. Data were collected from 24 research sites and 61 production systems through the Long-term Agroecosystem Research (LTAR) network and partner organizations spanning 22 U.S. states and 2 Canadian provinces. The objectives of this paper are to (a) present and provide a description of the P-FLUX dataset, (b) provide summary analyses of the agricultural production systems included in the dataset and the variability in P inputs and outputs across systems, and (c) provide details for accessing the dataset, dataset limitations, and an example of future use. P-FLUX includes information on select site characteristics (area, soil series), crop rotation, P inputs (P application rate, source, timing, placement, P in irrigation water, atmospheric deposition), P outputs (crop removal, hydrologic losses), P budgets (agronomic budget, overall budget), uncertainties associated with each flux and budget, and data sources. Phosphorus fluxes and budgets vary across agricultural production systems and are useful resources to improve P use efficiency and develop management strategies to mitigate environmental impacts of agricultural systems. P-FLUX is available for download through the USDA Ag Data Commons (https://doi.org/10.15482/USDA.ADC/1523365).

Effect of Planting into a Green Winter Cereal Rye Cover Crop on Growth and Development, Seedling Disease, and Yield of Corn.

Terminating winter cereal rye (Secale cereale L.) cover crops ≥10 days before planting (DBP) corn is recommended to minimize seedling disease and potential yield loss. In Iowa, cold temperatures and frequent precipitation can prevent farmers from following that recommendation and sometimes force them to plant corn while the rye plants are still green, referred to as "planting green" (PG). A field trial was established to evaluate the effect of rye termination shortly before or after corn planting on growth, seedling root disease, and yield of corn. A rye cover crop was terminated 17 and 3 DBP and 6 and 12 days after planting (DAP) corn; corn planted following no rye was included as a control. Rye biomass, C/N ratio, and N accumulation increased when terminated 6 or 12 DAP corn compared with rye terminated 17 or 3 DBP corn. Corn seedlings were taller from the PG treatments. More radicle root rot was observed when rye was terminated 3 DBP, 6 DAP, and 12 DAP corn than for the 17 DBP treatment and the no-rye control. Generally, greater Pythium clade B populations were detected on radicles and seminal roots of corn from the PG treatments. Corn populations, ears, or barren plants were not affected by the treatments. In both years, the no-rye control had the greatest corn yield and the 12 DAP treatment had the lowest yield. Our results suggest that PG increased corn seedling root disease and contributed to reduced corn yield.

Impact of hydraulic residence time on nitrate removal in pilot-scale woodchip bioreactors.

Nitrate (NO3-N) export from row crop agricultural systems with subsurface tile drainage continues to be a major water quality concern. Woodchip bioreactors are an effective edge-of-field practice designed to remove NO3-N from tile drainage. The NO3-N removal rate of woodchip bioreactors can be impacted by several factors, including hydraulic residence time (HRT). This study examined the impact of three HRTs, 2 h, 8 h, and 16 h, on NO3-N removal in a set of nine pilot-scale woodchip bioreactors in Central Iowa. NO3-N concentration reduction from the inlet to the outlet was significantly different for all HRTs (p < 0.05). The 16 h HRT removed the most NO3-N by concentration (7.5 mg L-1) and had the highest removal efficiency at 53.8%. The 8 h HRT removed an average of 5.5 mg L-1 NO3-N with a removal efficiency of 32.1%. The 2 h HRT removed an average of 1.3 mg L-1 NO3-N with a removal efficiency of 9.0%. The 2 h HRT had the highest NO3-N mass removal rate (MRR) at 9.0 g m-3 day-1, followed by the 8 h HRT at 8.5 g m-3 day-1, and the 16 h HRT at 7.4 g m-3 day-1, all of which were statistically different (p < 0.05). Significant explanatory variables for removal efficiency were HRT (p < 0.001) and influent NO3-N concentration (p < 0.001), (R2 = 0.80), with HRT accounting for 93% contribution. When paired with results from a companion study, the ideal HRT for the bioreactors was 8 h to achieve maximum NO3-N removal while reducing the impact from greenhouse gas emissions.

Advancing the Sustainability of US Agriculture through Long-Term Research.

Agriculture in the United States must respond to escalating demands for productivity and efficiency, as well as pressures to improve its stewardship of natural resources. Growing global population and changing diets, combined with a greater societal awareness of agriculture's role in delivering ecosystem services beyond food, feed, fiber, and energy production, require a comprehensive perspective on where and how US agriculture can be sustainably intensified, that is, made more productive without exacerbating local and off-site environmental concerns. The USDA's Long-Term Agroecosystem Research (LTAR) network is composed of 18 locations distributed across the contiguous United States working together to integrate national and local agricultural priorities and advance the sustainable intensification of US agriculture. We explore here the concept of sustainable intensification as a framework for defining strategies to enhance production, environmental, and rural prosperity outcomes from agricultural systems. We also elucidate the diversity of factors that have shaped the past and present conditions of cropland, rangeland, and pastureland agroecosystems represented by the LTAR network and identify priorities for research in the areas of production, resource conservation and environmental quality, and rural prosperity. Ultimately, integrated long-term research on sustainable intensification at the national scale is critical to developing practices and programs that can anticipate and address challenges before they become crises.

Assessing Pathogen Presence in an Intensively Tile Drained, Agricultural Watershed.

Increases in swine production and concomitant manure application provide beneficial nutrients for crops but also include the potential to spread pathogenic bacteria in the environment. While manure is known to contain a variety of pathogens, little is known regarding the long-term effect of manure application on fate and transport of this diverse set of pathogens into surrounding waterways. We report on the use of 16S-rRNA gene sequencing to detect pathogen-containing genera in the agriculturally dominated South Fork Iowa River watershed, home to approximately 840,000 swine in the 76,000-ha basin. DNA was extracted from monthly grab samples collected from three surface water sites and two main artificial drainage outlets. DNA sequences from water samples were matched with sequences from genera known to contain pathogens using targeted 16S rRNA amplicon sequencing. The specific genera known to contain pathogens were quantified by combining percentage of genera sequence matches with 16S rRNA gene quantitative polymerase chain reaction results. Specifically, abundances of , , and significantly increased in surface water after typical fall manure application. Additionally, the likely transport pathways for specific genera known to contain pathogens were identified. Surface water concentrations were influenced mainly by artificial drainage, whereas was primarily transported to surface waters by runoff events. The results of this study will help us to understand environmental pathways that may be useful for mitigation of the diverse set of pathogenic genera transported in agroecosystems and the capability of manure application to alter existing microbial community structures.

Time Interval Between Cover Crop Termination and Planting Influences Corn Seedling Disease, Plant Growth, and Yield.

Experiments were established in a controlled-growth chamber and in the field to evaluate the effect of the length of time intervals between winter rye cover crop termination and corn planting on corn seedling disease, corn growth, and grain yield in 2014 and 2015. Rye termination dates ranged from 25 days before planting (DBP) to 2 days after planting (DAP) corn in the field and from 21 DBP to 1 DAP in controlled studies. Results were similar in both environments. In general, shorter intervals increased seedling disease and reduced corn emergence, shoot growth, and grain yield of corn following winter rye compared with corn planted 10 or more days after rye termination or without rye. Incidence of Pythium spp. increased with shorter intervals (less than 8 DBP); incidence of Fusarium spp. was not consistent between runs and experiments. In 2014, in the 1-DAP treatment, number of ears and grain yield were reduced (P = 0.05 and 0.02, respectively). In 2015, all termination intervals reduced plant population, number of ears, and yield (P = 0.01), with the 2-DBP treatment causing the biggest decrease. A 10- to 14-day interval between rye termination and corn planting should be followed to improve corn yield following a rye cover crop.

Effects of tillage and poultry manure application rates on Salmonella and fecal indicator bacteria concentrations in tiles draining Des Moines Lobe soils.

Application of poultry manure (PM) to cropland as fertilizer is a common practice in artificially drained regions of the Upper Midwest United States. Tile-waters have the potential to contribute pathogenic bacteria to downstream waters. This 3-year study (2010-2012) was designed to evaluate the impacts of manure management and tillage practices on bacteria losses to drainage tiles under a wide range of field conditions. PM was applied annually in spring, prior to planting corn, at application rates ranging from 5 to 40 kg/ha to achieve target rates of 112 and 224 kg/ha nitrogen (PM1 and PM2). Control plots received no manure (PM0). Each treatment was replicated on three chisel-plowed (CP) plots and one no-till (NT) plot. Tile-water grab samples were collected weekly when tiles were flowing beginning 30 days before manure application to 100 days post application, and additional grab samples were obtained to target the full spectrum of flow conditions. Manure and tile-water samples were analyzed for the pathogen, Salmonella spp. (SALM), and fecal indicator bacteria (FIB), Escherichia coli (EC), and enterococci (ENT). All three bacterial genera were detected more frequently, and at significantly higher concentrations, in tile-waters draining NT plots compared to CP plots. Transport of bacteria to NT tiles was most likely facilitated by macropores, which were significantly more numerous above tiles in NT plots in 2012 as determined by smoke-testing. While post-manure samples contained higher concentrations of bacteria than pre-manure samples, significant differences were not seen between low (PM1) and high (PM2) rates of PM application. The highest concentrations were observed under the NT PM2 plot in 2010 (6.6 × 10(3) cfu/100 mL EC, 6.6 × 10(5) cfu/100 mL ENT, and 2.8 × 10(3) cfu/100 mL SALM). Individual and 30-day geometric mean ENT concentrations correlated more strongly to SALM than EC; however, SALM were present in samples with little or no FIB.

Dissipation of atrazine, enrofloxacin, and sulfamethazine in wood chip bioreactors and impact on denitrification.

Wood chip bioreactors are receiving increasing attention as a means of reducing nitrate in subsurface tile drainage systems. Agrochemicals in tile drainage water entering wood chip bioreactors can be retained or degraded and may affect denitrification. The degradation of 5 mg L atrazine, enrofloxacin, and sulfamethazine under denitrifying conditions in wood chips from an in situ reactor was determined. The impact of these chemicals on denitrifying microorganisms was assessed using the denitrification potential assay, most probable number (MPN), and quantitative polymerase chain reaction targeting the gene of the denitrifiers. Initial half-lives for these chemicals in the aqueous phase were 0.98 d for atrazine, 0.17 d for enrofloxacin, and 6.2 d for sulfamethazine. Similar rates of disappearance in autoclaved and nonautoclaved wood chip solutions during the first 48 h suggested sorption was the dominant mechanism. The presence of atrazine did not impair denitrification potential, the MPN, or the copy number. The denitrifier MPN and copy number in sulfamethazine- and enrofloxacin-treated microcosms were less than the control within the first 5 d after chemical addition, whereas the denitrification potentials were not affected. However, after 45 d the denitrification rate, MPN and gene copy numbers for sulfamethazine and enrofloxacin were similar to that of the no-chemical control, indicating that acclimation of the denitrifier population to the antibiotic or reduced bioavailability over time allowed recovery of the denitrifier population.

Swine manure injection with low-disturbance applicator and cover crops reduce phosphorus losses.

Injection of liquid swine manure disturbs surface soil so that runoff from treated lands can transport sediment and nutrients to surface waters. We determined the effect of two manure application methods on P fate in a corn (Zea mays L.)-soybean [Glycine max (L.) Merr.] production system, with and without a winter rye (Secale cereale L.)-oat (Avena sativa L.) cover crop. Treatments included: (i) no manure; (ii) knife injection; and (iii) low-disturbance injection, each with and without the cover crop. Simulated rainfall runoff was analyzed for dissolved reactive P (DRP) and total P (TP). Rainfall was applied 8 d after manure application (early November) and again in May after emergence of the corn crop. Manure application increased soil bioavailable P in the 20- to 30-cm layer following knife injection and in the 5- to 20-cm layer following low-disturbance injection. The low-disturbance system caused less damage to the cover crop, so that P uptake was more than threefold greater. Losses of DRP were greater in both fall and spring following low-disturbance injection; however, application method had no effect on TP loads in runoff in either season. The cover crop reduced fall TP losses from plots with manure applied by either method. In spring, DRP losses were significantly higher from plots with the recently killed cover crop, but TP losses were not affected. Low-disturbance injection of swine manure into a standing cover crop can minimize plant damage and P losses in surface runoff while providing optimum P availability to a subsequent agronomic crop.

Source-pathway separation of multiple contaminants during a rainfall-runoff event in an artificially drained agricultural watershed.

A watershed's water quality is influenced by contaminant-transport pathways unique to each landscape. Accurate information on contaminant-pathways could provide a basis for mitigation through well-targeted approaches. This study determined dynamics of nitrate-N, total P, Escherichia coli, and sediment during a runoff event in Tipton Creek, Iowa. The watershed, under crop and livestock production, has extensive tile drainage discharging through an alluvial valley. A September 2006 storm yielded 5.9 mm of discharge during the ensuing 7 d, which was monitored at the outlet (19,850 ha), two tile-drainage outfalls (total 1856 ha), and a runoff flume (11 ha) within the sloped valley. Hydrograph separations indicated 13% of tile discharge was from surface intakes. Tile and outlet nitrate-N loads were similar, verifying subsurface tiles dominate nitrate delivery. On a unit-area basis, tile total P and E. coli loads, respectively, were about half and 30% of the outlet's; their rapid, synchronous timing showed surface intakes are an important pathway for both contaminants. Flume results indicated field runoff was a significant source of total P and E. coli loads, but not the dominant one. At the outlet, sediment, P, and E. coli were reasonably synchronous. Radionuclide activities of (7)Be and (210)Pb in suspended sediments showed sheet-and-rill erosion sourced only 22% of sediment contributions; therefore, channel sources dominated and were an important source of P and E. coli. The contaminants followed unique pathways, necessitating separate mitigation strategies. To comprehensively address water quality, erosion-control and nitrogen-management practices currently encouraged could be complemented by buffering surface intakes and stabilizing stream banks.

Rye cover crop and gamagrass strip effects on NO3 concentration and load in tile drainage.

A significant portion of the NO3 from agricultural fields that contaminates surface waters in the Midwest Corn Belt is transported to streams or rivers by subsurface drainage systems or "tiles." Previous research has shown that N fertilizer management alone is not sufficient for reducing NO3 concentrations in subsurface drainage to acceptable levels; therefore, additional approaches need to be devised. We compared two cropping system modifications for NO3 concentration and load in subsurface drainage water for a no-till corn (Zea mays L.)-soybean (Glycine max [L.] Merr.) management system. In one treatment, eastern gamagrass (Tripsacum dactyloides L.) was grown in permanent 3.05-m-wide strips above the tiles. For the second treatment, a rye (Secale cereale L.) winter cover crop was seeded over the entire plot area each year near harvest and chemically killed before planting the following spring. Twelve 30.5x42.7-m subsurface-drained field plots were established in 1999 with an automated system for measuring tile flow and collecting flow-weighted samples. Both treatments and a control were initiated in 2000 and replicated four times. Full establishment of both treatments did not occur until fall 2001 because of dry conditions. Treatment comparisons were conducted from 2002 through 2005. The rye cover crop treatment significantly reduced subsurface drainage water flow-weighted NO3 concentrations and NO3 loads in all 4 yr. The rye cover crop treatment did not significantly reduce cumulative annual drainage. Averaged over 4 yr, the rye cover crop reduced flow-weighted NO3 concentrations by 59% and loads by 61%. The gamagrass strips did not significantly reduce cumulative drainage, the average annual flow-weighted NO3 concentrations, or cumulative NO3 loads averaged over the 4 yr. Rye winter cover crops grown after corn and soybean have the potential to reduce the NO3 concentrations and loads delivered to surface waters by subsurface drainage systems.

Sorption of tylosin onto swine manure.

Sorption of tylosin was conducted on manure solids (<2 mm) and colloidal materials (<1.2 microm) collected from open (OL) and covered (CL) anaerobic swine manure lagoons. The aqueous concentration of tylosin in the sorption studies bracket the levels expected in lagoons, between 1 mgl(-1) and 30 mgl(-1). Sorption isotherms were found to be slightly non-linear for 2 mm solids, with Freundlich distribution coefficients (K(f)) of 39.4 with n=1.32 for CL slurry and 99.5 with n=1.02 for OL. These values are comparable to those reported for loam soils, but higher than those reported for sandy or clay soils and lower than those reported for fresh manure. Normalization of K(d) to the organic carbon content of the solids gave K(oc) values of 570 lkg(-1) and 818 lkg(-1), for CL and OL solids, respectively. The K(d) and K(f) values were not significantly different between colloids and 2 mm solids in OL slurry, but were significantly different in CL due to the non-linearity of the colloid isotherm. Based on the K(d) values obtained and comparing the K(d) values of other antibiotics, tylosin is strongly sorbed to manure, and would be more mobile than tetracyclines, but less mobile than sulfonamides, olaquindox, and chloramphenicol. However, tylosin mobility may be facilitated through transport with colloidal manure materials.

Degradation and metabolite production of tylosin in anaerobic and aerobic swine-manure lagoons.

Watershed contamination from antibiotics is becoming a critical issue because of increased numbers of confined animal-feeding operations and the use of antibiotics in animal production. To understand the fate of tylosin in manure before it is land-applied, degradation in manure lagoon slurries at 22 degrees C was studied. Tylosin disappearance followed a biphasic pattern, where rapid initial loss was followed by a slow removal phase. The 90% disappearance times for tylosin, relomycin (tylosin D), and desmycosin (tylosin B) in anaerobically incubated slurries were 30 to 130 hours. Aerating the slurries reduced the 90% disappearance times to between 12 and 26 hours. Biodegradation and abiotic degradation occur, but strong sorption to slurry solids was probably the primary mechanism of tylosin disappearance. Dihydrodesmycosin and an unknown degradate with molecular mass of m/z 934.5 were detected. Residual tylosin remained in slurry after eight months of incubation, indicating that degradation in lagoons is incomplete and that residues will enter agricultural fields.

Biodegradation of atrazine by Agrobacterium radiobacter J14a and use of this strain in bioremediation of contaminated soil.

We examined the ability of a soil bacterium, Agrobacterium radiobacter J14a, to degrade the herbicide atrazine under a variety of cultural conditions, and we used this bacterium to increase the biodegradation of atrazine in soils from agricultural chemical distribution sites. J14a cells grown in nitrogen-free medium with citrate and sucrose as carbon sources mineralized 94% of 50 microgram of [14C-U-ring]atrazine ml-1 in 72 h with a concurrent increase in the population size from 7.9 x 10(5) to 5.0 x 10(7) cells ml-1. Under these conditions cells mineralized the [ethyl-14C]atrazine and incorporated approximately 30% of the 14C into the J14a biomass. Cells grown in medium without additional carbon and nitrogen sources degraded atrazine, but the cell numbers did not increase. Metabolites produced by J14a during atrazine degradation include hydroxyatrazine, deethylatrazine, and deethyl-hydroxyatrazine. The addition of 10(5) J14a cells g-1 into soil with a low indigenous population of atrazine degraders treated with 50 and 200 microgram of atrazine g-1 soil resulted in two to five times higher mineralization than in the noninoculated soil. Sucrose addition did not result in significantly faster mineralization rates or shorten degradation lag times. However, J14a introduction (10(5) cells g-1) into another soil with a larger indigenous atrazine-mineralizing population reduced the atrazine degradation lag times below those in noninoculated treatments but did not generally increase total atrazine mineralization.

The frequency of Sarcocystis spp. and its effect on winter carcass composition of mottled ducks.

Macrocysts of the protozoan parasite Sarcocystis spp. occurred in 34 of 241 (14%) adult and 6 of 70 (8.6%) immature mottled ducks (Anas fulvigula) that were collected in southwestern Louisiana from 1987 to 1989. The prevalence of Sarcocystis spp. among immature ducks was higher than previously reported, possibly because of a longer exposure period. No differences in carcass composition variables were found between heavily infected and uninfected adult mottled ducks during the winter period of the annual cycle. We concluded that macrocysts of Sarcocystis spp. did not influence carcass composition and probably exert negligible effects on survival of parasitized mottled ducks during winter.