Modulation of the gut microbiome and Firmicutes phylum reduction by a nutraceutical blend in the obesity mouse model and overweight humans: A double-blind clinical trial.

Overweight and obesity are closely linked to gut dysbiosis/dysmetabolism and disrupted De-Ritis ratio [aspartate aminotransferase (AST)/alanine aminotransferase (ALT) ratio], which may contribute to chronic noncommunicable diseases onset. Concurrently, extensive research explores nutraceuticals, and health-enhancing supplements, for disease prevention or treatment. Thus, sedentary overweight volunteers were double-blind randomized into two groups: Novel Nutraceutical_(S) (without silymarin) and Novel Nutraceutical (with silymarin). Experimental formulations were orally administered twice daily over 180 consecutive days. We evaluated fecal gut microbiota, based on partial 16S rRNA sequences, biochemistry and endocrine markers, steatosis biomarker (AST/ALT ratio), and anthropometric parameters. Post-supplementation, only the Novel Nutraceutical group reduced Clostridium clostridioforme (Firmicutes), Firmicutes/Bacteroidetes ratio (F/B ratio), and De-Ritis ratio, while elevating Bacteroides caccae and Bacteroides uniformis (Bacteroidetes) in Brazilian sedentary overweight volunteers after 180 days. In summary, the results presented here allow us to suggest the gut microbiota as the action mechanism of the Novel Nutraceutical promoting metabolic hepatic recovery in obesity/overweight non-drug interventions.

Persistent hypofibrinolysis in severe COVID-19 associated with elevated fibrinolysis inhibitors activity.

Hypercoagulability and reduced fibrinolysis are well-established complications associated with COVID-19. However, the timelines for the onset and resolution of these complications remain unclear. The aim of this study was to evaluate, in a cohort of COVID-19 patients, changes in coagulation and fibrinolytic activity through ROTEM assay at different time points during the initial 30 days following the onset of symptoms in both mild and severe cases. Blood samples were collected at five intervals after symptoms onset: 6-10 days, 11-15 days, 16-20 days, 21-25 days, and 26-30 days. In addition, fibrinogen, plasminogen, PAI-1, and alpha 2-antiplasmin activities were determined. Out of 85 participants, 71% had mild COVID-19. Twenty uninfected individuals were evaluated as controls. ROTEM parameters showed a hypercoagulable state among mild COVID-19 patients beginning in the second week of symptoms onset, with a trend towards reversal after the third week of symptoms. In severe COVID-19 cases, hypercoagulability was observed since the first few days of symptoms, with a tendency towards reversal after the fourth week of symptoms onset. A hypofibrinolytic state was identified in severe COVID-19 patients from early stages and persisted even after 30 days of symptoms. Elevated activity of PAI-1 and alpha 2-antiplasmin was also detected in severe COVID-19 patients. In conclusion, both mild and severe cases of COVID-19 exhibited transient hypercoagulability, reverted by the end of the first month. However, severe COVID-19 cases sustain hypofibrinolysis throughout the course of the disease, which is associated with elevated activity of fibrinolysis inhibitors. Persistent hypofibrinolysis could contribute to long COVID-19 manifestations.

Consumers' Segmentation Influences Acceptance and Preferences of Cheeses with Sanitary Inspection and Artisanal Seals.

Food labeling serves as a crucial medium for industries to communicate product qualities to consumers. Sanitary inspection and artisanal seals are significant markers for traditional cheeses, yet current information on this topic is limited. Therefore, this study aims to evaluate the impact of sanitary inspection and the ARTE seal on the acceptance of artisanal cheese. To achieve this objective, four hypothetical cheese labels featuring all combinations of sanitary inspection and ARTE seals were presented to 404 consumers. These consumers rated their acceptance of each label, a conjoint analysis was conducted, and the relative importance of each seal was calculated. Subsequently, consumers were segmented using hierarchical cluster analysis. Their socio-demographic profiles were statistically correlated to the clusters through a chi-squared method. The results revealed the existence of three distinct consumer groups: those who strongly prefer cheeses with a sanitary seal (assigning a relative importance of 80.2% to the seal), those who favor cheeses with an artisanal seal (assigning a relative importance of 52.5% to the seal), and those for whom the presence of either seal did not significantly affect acceptance. Consumers residing in metropolitan areas generally placed less value on both seals, whereas frequent purchasers of artisanal foods and residents of rural areas showed a preference for the artisanal seal. Other socio-demographic variables did not statistically correlate with cluster membership. In conclusion, consumer segmentation based on preferences for sanitary inspection and artisanal seals in food labeling is vital for developing effective marketing strategies and food safety education policies.

Onde Vivem os Monstros? Enlaces entre Cinema, Adolescência e Horror / Where do the Monsters Live? Links Between Cinema, Adolescence and Horror / ¿Dónde Viven Los Monstruos? Vínculos Entre Cine, Adolescencia y Horror

Partindo da ideia de Stephen King de que as produções de horror servem como um barômetro muito preciso que aponta aquilo que aterroriza uma sociedade, este artigo tem como objetivo propor uma reflexão acerca dos enlaces entre adolescência, cinema e horror, no momento em que a adolescência como categoria social se consolida, isto é, nos anos 1950. Para isso, realizamos uma revisão bibliográfica sobre a relação entre horror, monstruosidade e cultura, assim como sobre o desenvolvimento dos gêneros cinematográficos horror e teen movies. Também realizamos sucintas análises fílmicas de algumas obras importantes na história desses gêneros cinematográficos. Observamos que, se, por um lado, com o surgimento dos teen movies, as telas do cinema passam a ser habitadas por monstros adolescentes, por outro, os discursos com pretensão de cientificidade nos falam sobre uma adolescência monstruosa. A partir disso, concluímos que talvez haja uma intrigante equivalência cultural entre adolescência e monstruosidade: tanto monstros quanto adolescentes trazem à luz algumas questões fundamentais para o ser humano, encarnam aquilo para o que não temos resposta, apontam os limites do saber.

Based upon Stephen King's idea that the horror productions act as a barometer, pointing out what terrifies some societies, this article aims to propose some thoughts about the possible links among adolescence, cinema, and horror, around the time adolescence emerges as a consolidated social category (in the 1950s). In order to achieve that, we researched the relations between horror, monstrosity, culture, and the development of the horror and teen movies cinematographic genres. We also made a brief film analysis of some important works in the history of these cinematographic genres. We notice that two events occurred in parallel: the emergence of teenage monsters in teen movies on the cinema screens and the allegedly scientific discourses about a monstrous adolescence. We have concluded that perhaps there is a cultural equivalence between adolescence and monstrosity: monsters and teenagers bring to light some fundamental questions for the human being; they incorporate things to which we do not have an answer and point out the limits of our knowledge.

Partiendo de la idea de Stephen King de que las producciones de terror sirven como un barómetro muy preciso que señala lo que aterroriza a una sociedad, este artículo pretende proponer una reflexión sobre los vínculos entre la adolescencia, el cine y el terror, en el momento en el que la adolescencia se consolida como una categoría social, es decir, en la década de 1950. Para ello, realizamos un repaso sobre la relación entre horror, monstruosidad y cultura, así como sobre el desarrollo de los géneros cinematográficos horror y teen movies. También hicimos un breve análisis fílmico de algunas películas importantes en la historia de estos géneros cinematográficos. Observamos que, por un lado, con la aparición de los teen movies, las pantallas de cine ahora están habitadas por monstruos adolescentes, por otro, los discursos con pretensión de cientificidad nos hablan de una adolescencia monstruosa. De esto, concluimos que puede haber una equivalencia cultural intrigante entre la adolescencia y la monstruosidad: tanto los monstruos como los adolescentes sacan a la luz algunas preguntas fundamentales para los seres humanos, encarnan aquello para lo que no tenemos respuesta, señalan los límites del saber.

Evaluation of cleaning and sterilization of liposuction cannulas after intentional contamination with human fat, Mycobacterium abscessus subspecies bolletii, and Geobacillusstearothermophilus.

BACKGROUND: The complex design of cannulas is a major challenge for reprocessing due to the retention and accumulation of fat residues. AIM: To evaluate the cleaning of liposuction cannulas and the protective effects of the remaining fat residues on Mycobacterium abscessus subspecies bolletii (MASB) and Geobacillus stearothermophilus inactivation during steam sterilization. METHODS: In phase 1, six standard operating procedures were evaluated for cleaning of liposuction cannulas. In phase 2, the sectioned lumens of the liposuction cannulas were contaminated with the largest and smallest volume of human fat found in phase 1, with the addition of MASB. In phase 3, the same volumes of human fat from phase 2 were used to contaminate paper strips containing G. stearothermophilus. FINDINGS: In phase 1 the residual amount of fat ranged from 6 to 52 mg. In phases 2 and 3, the minimum and maximum amounts of fat (6 and 50 mg) protected micro-organisms during steam sterilization at 134 °C for 1.5 and 3 min. CONCLUSION: Effective cleaning and sterilization of liposuction cannulas intentionally contaminated with human fat, MASB and G. stearothermophilus could not be performed.

Análise da situação de saúde dos idosos usuários de uma política municipal de atividades físicas / Analysis of health status of older adults users of a municipal physical activity program / Análisis de la situación de salud de los adultos ancianos de una política municipal de actividad física

Introdução: Políticas públicas intersetoriais em saúde são intervenções populacionais (e de cunho ecológico) muito utilizadas para a redução da carga global de doença e otimização de recursos tanto financeiros quanto humanos. Objetivo: O objetivo deste estudo foi analisar a situação de saúde de usuários idosos de uma política municipal de atividades físicas. Métodos: Trata-se de um estudo transversal com amostragem baseada em centros comunitários (N dispositivos comunitários=11), que disponibilizam práticas de movimentos corporais e outros, subsidiados pela Secretaria Municipal de Desenvolvimento Social e Esporte (SMDSE), Prefeitura Municipal de Porto Alegre (RS). A amostragem de usuários foi ponderada para o total de usuários atendidos por centro, adotando seleção aleatória simples. A coleta de dados ocorreu entre abril de 2018 e fevereiro de 2019, em que a equipe de coleta se deslocou ao território adscrito dos usuários para a condução de inquérito de saúde autoaplicado e a avaliação funcional; de forma contrária, os usuários compareceram a um centro de coleta para a série laboratorial (sem jejum). Resultados: Foram incluídos e analisados 351 usuários (média±desvio padrão, 70±6 anos). Para fatores de risco cardiovasculares, a prevalência de hipercolesterolemia foi de 54,2% e de 49,3% para hipertensão arterial sistêmica ­ as mais elevadas. O transtorno de sono foi prevalente em 55,3% da amostra. Entre as doenças autorrelatadas, os participantes listaram as cardiovasculares (14,3%), câncer prévio (14,6%), diabetes (13,2%), artrite reumatoide/ reumatismo (29,6%) e depressão (sem discriminador de depressão maior/ menor) (18,6%). A capacidade funcional, estimada pelo teste de caminhada em 6 minutos e a força de preensão manual, preditores de mortalidade cardiovascular e agravos, tiveram valores médios encontrados de 498,05±78,96 m e 27,08±8,14 kg, respectivamente. Conclusão: Os achados do presente estudo permitem contrastar prevalências estimadas em idosos participantes de um programa público de atividades físicas com outras estimativas em grupos de comparação, possibilitando a análise de situação de saúde com base em diferentes comportamentos e fatores de risco. Por fim, o trabalho viabilizou a monitorização de intervenções públicas para idosos em nível comunitário, sendo um ponto de base para acompanhamento futuro.

Introduction: Intersectoral public policies on health are population (and ecological) interventions widely used to reduce the global burden of disease and optimize both financial and human resources. Objective: The objective of this study was to analyze the health status of older adults users of a municipal policy on physical activities. Methods: This is a cross-sectional study with a sample based on community centers (N community centers=11), which provide body movement practices and others, funded by the Municipal Department of Social Development and Sports (SMDSE), City Hall of Porto Alegre (state of Rio Grande do Sul ­ RS, Brazil). The users' sample was weighted by the number of users of each center, by simple random sampling. Data collection took place between April 2018 and February 2019, in which the collection team went to the users' assigned territory to conduct a self-administered health survey and functional assessment; conversely, the users attended a collection center for laboratory tests (without fasting). Results: A total of 351 users (mean±SD, 70±6 years old) were included. For cardiovascular risk factors, the prevalence of hypercholesterolemia was 54.2% and 49.3% for systemic arterial hypertension ­ the highest levels. Sleep disorder was prevalent in 55.3% of the sample. Among the self-reported diseases, participants listed cardiovascular (14.3%), previous cancer (14.6%), diabetes (13.2%), rheumatoid arthritis/rheumatism (29.6%), and depression (without major/minor depression discrimination) (18.6%). Functional capacity, estimated by the six-minute walk test, and handgrip strength, predictors of cardiovascular mortality and injuries, had mean values of 498.05±78.96 m and 27.08±8.14 kg, respectively. Conclusions: The findings of this study allow contrasting prevalence rates estimated in older adults participants of a public physical activity program with other estimates in comparison groups, enabling the analysis of health status based on different behaviors and risk factors. All in all, our study enabled to monitor public interventions for older adults at the community level, serving as a baseline for future monitoring.

Introducción: Las políticas de salud pública intersectoriales tienen el potencial de destacar la reducción de la carga global de enfermedad y la optimización de los recursos financieros y humanos. Objetivo: El objetivo de este estudio fue analizar la situación de salud de los usuarios, ancianos, de una política municipal de actividades físicas. Métodos: Se trata de un estudio transversal con muestreo a partir de centros comunitarios (N dispositivos comunitarios=11), que brindan prácticas de movimiento corporal y otras, subsidiadas por la Secretaria Municipal de Desenvolvimento Social e Esporte (SMDSE), Prefeitura Municipal de Porto Alegre (RS). El muestreo de usuarios fue ponderado por el total de usuarios atendidos por centro, adoptando selección aleatoria simple. La recolección de datos ocurrió entre abril/2018 y febrero/2019, cuando el equipo de recolección viajó al territorio asignado de los usuarios para realizar una encuesta de salud auto aplicada y evaluación funcional; por el contrario, los usuarios acudían a un centro de recogida de series de laboratorio (sin ayuno). Resultados: Se incluyeron y analizaron 351 usuarios (media±DP, 70±6 años). Para los factores de riesgo cardiovascular, la prevalencia de hipercolesterolemia fue de 54,2% y de hipertensión arterial sistémica de 49,3%, la más alta. El trastorno del sueño fue prevalente en el 55,3% de la muestra. Entre las enfermedades auto informadas, los participantes enumeraron enfermedades cardiovasculares (14,3%), cáncer previo (14,6%), diabetes (13,2%), artritis reumatoide/reumatismo (29,6%) y depresión (sin discriminación de depresión mayor/menor) (18,6%). La capacidad funcional, estimada por la prueba de la marcha de 6 minutos, y la fuerza de prensión manual, predictores de mortalidad y lesiones cardiovasculares, tuvieron valores medios de 498,05±78,96 m y 27,08±8,14 kg, respectivamente. Conclusiones: Los hallazgos de este estudio permiten contrastar las prevalencias estimadas en participantes ancianos de un programa público de actividades físicas con otras estimaciones en grupos de comparación, lo que permite el análisis de la situación de salud basado en diferentes comportamientos y factores de riesgo. Por último, el trabajo ha facilitado la monitorización de intervenciones públicas para personas mayores a nivel comunitario, sirviendo como punto de partida para un seguimiento futuro.

Prognostic value of myosteatosis and systemic inflammation in patients with resectable gastric cancer: A retrospective study.

BACKGROUND/OBJECTIVES: The association between systemic inflammation and myosteatosis upon diagnosis of gastric cancer (GC) and whether these factors could predict survival outcomes is not clear. Our aim was to explore the association between systemic inflammation and myosteatosis upon diagnosis of GC, specially whether the co-occurrence of these factors could predict survival outcomes. SUBJECTS/METHODS: Computed tomography (CT) was performed at the level of the third lumbar vertebra for body composition analysis in 280 patients with GC. Myoesteatosis was defined as the lowest tertile of the muscle radiodensity distribution or based on clinical significance using optimal stratification analysis. Inflammatory indexes were measured, including the neutrophil-to-lymphocyte (NLR), platelet-to-lymphocyte and lymphocyte-to-monocyte ratios. RESULTS: Patients with low skeletal muscle (SM) radiodensity were more likely to be older than 65 years, have a higher body mass index and have diabetes. They also had higher intermuscular visceral and subcutaneous adipose tissue areas and indexes. The highest tertile of SM radiodensity was associated with better disease-free survival (DFS) (HR = 0.51, 95% CI [0.31, 0.84], ptrend = 0.020) and overall survival (OS) (HR = 0.49, 95% CI [0.29, 0.82], ptrend = 0.022). Patients with NLR > 2.3 and myosteatosis had the worst DFS and OS (HR = 2.77, 95% CI [1.54, 5.00], p = 0.001; HR = 3.31, 95% CI [1.79, 6.15], p < 0.001, respectively). CONCLUSION: Co-occurrence of myosteatosis and inflammation increased disease progression and death risk by almost three times. These regularly obtained biomarkers might improve prognostic risk prediction in resectable GC.

Encaminhamento e resolutividade da consultoria de aleitamento materno em uma unidade de alojamento conjunto / Referral and resolution of breastfeeding consultancy in a joint accommodation unit / Derivación y resolución de consultoría en lactancia materna en unidad de alojamiento conjunta

Objetivo: caracterizar os encaminhamentos e a resolutividade da consultoria em aleitamento materno em uma unidade de alojamento conjunto. Método: estudo transversal descritivo realizado com 231 puérperas e seus recém-nascidos internados em alojamento conjunto no sul do Brasil. A coleta de dados foi realizada no período de agosto de 2016 a maio de 2017. Os dados foram analisados mediante estatística descritiva. Resultados: os motivos prevalentes para o encaminhamento foram dificuldade na técnica de amamentação (81,7%), primiparidade (57,8%), anatomia mamária (28,7%), presença de fissuras mamilares (19,6%) e dor ao amamentar (18,3%). A satisfação com o atendimento foi relatada por 97,8% das puérperas. Quanto a resolutividade, 70,6% consideraram o atendimento totalmente resolutivo, 26,4% parcialmente e 3,0% não resolutivo. Conclusão: a consultoria em aleitamento materno foi resolutiva na maioria dos encaminhamentos, motivados por dificuldades que poderiam predispor uma interrupção precoce da amamentação, demonstrando a eficácia da inserção desse profissional nos serviços de saúde.

Objective: to characterize the referrals and resoluteness of breastfeeding consultancy in a rooming-in unit. Method: descriptive cross-sectional study conducted with 231 postpartum women and their newborns hospitalized in rooming-in in southern Brazil. Data collection was carried out from August 2016 to May 2017. Data were analyzed using descriptive statistics. Results: the prevalent reasons for referral were difficulty in the breastfeeding technique (81.7%), primiparity (57.8%), breast anatomy (28.7%), presence of cracked nipples (19.6%) and pain when breastfeeding (18.3%). Satisfaction with the service was reported by 97.8% of the mothers. As for resoluteness, 70.6% considered the service fully resolute, 26.4% partially and 3.0% non-resolutive. Conclusion:breastfeeding consultancy was resolute in most referrals, motivated by difficulties that could predispose to an early interruption of breastfeeding, demonstrating the effectiveness of the insertion of this professional in health services.

Objetivo: caracterizar las derivaciones y la determinación de la asesoría en lactancia materna en una unidad de alojamiento conjunto. Método: estudio descriptivo transversal realizado con 231 puérperas y sus recién nacidos hospitalizados en alojamiento conjunto en el sur de Brasil. La recolección de datos se llevó a cabo desde agosto de 2016 a mayo de 2017. Los datos se analizaron mediante estadística descriptiva. Resultados: los motivos prevalentes de derivación fueron dificultad en la técnica de lactancia (81,7%), primiparidad (57,8%), anatomía mamaria (28,7%), presencia de pezones agrietados (19,6%) y dolor al amamantar (18,3%). El 97,8% de las madres informó satisfacción con el servicio. En cuanto a la resolución, el 70,6% consideró el servicio plenamente resuelto, el 26,4% parcialmente y el 3,0% no resolutivo. Conclusión: la asesoría en lactancia materna fue resuelta en la mayoría de las derivaciones, motivada por dificultades que pudieran predisponer a una interrupción temprana de la lactancia materna, demostrando la efectividad de la inserción de esta profesional en los servicios de salud.

Humoral immunity is not altered in overweight pregnant Crioulo mares / Imunidade humoral não é alterada em éguas Crioulas gestantes com sobrepeso

Both pregnancy and obesity can influence significant changes in the immune system. On this basis, the present study proposes to evaluate the humoral immune response of overweight pregnant mares in response to a commercial vaccine. Thirty pregnant Crioulo mares were separated according to body condition score (BCS) into overweight (BCS≥7/9) or lean-control (BCS= 5-6/9). In each group, the animals were subdivided into vaccinated and controls. The mares were vaccinated against EHV-1 in two doses spaced 21 days apart and had their blood collected monthly, for five months, for antibody evaluation. Both vaccinated groups had an increase in specific neutralizing antibodies after the vaccine. However, after the second dose, there was no increase in antibodies in any of the groups. Vaccinated overweight and lean-control mares did not differ at any time point. Therefore, this study demonstrated that obesity does not influence the humoral immune response in pregnant Crioulo mares.(AU)

Tanto a gestação quanto a obesidade podem influenciar o desenvolvimento de alterações significativas no sistema imune, portanto, o presente estudo teve como objetivo avaliar a resposta imune humoral de éguas gestantes com sobrepeso em resposta a uma vacina comercial. Trinta éguas Crioulas gestantes foram separadas de acordo com o escore de condição corporal (ECC) em éguas com sobrepeso (ECC≥7/9) e éguas controles (ECC=5-6/9) e, ainda, em cada grupo, os animais também foram separados em vacinados e controles. As éguas foram vacinadas contra o EHV-1 em duas doses com intervalo de 21 dias, sendo realizadas coletas de sangue mensalmente durante cinco meses para avaliação de anticorpos neutralizantes. Ambos os grupos vacinados tiveram aumento de anticorpos neutralizantes específicos após a vacina, porém, após a segunda dose, não foi observado aumento de anticorpos em nenhum dos grupos. Nenhuma diferença foi observada entre éguas vacinadas com sobrepeso e as éguas controles em nenhum momento. Assim, este estudo demonstrou que a obesidade não é um fator que influencia a resposta imune humoral de éguas Crioulas gestantes.(AU)

Synbiotic Supplementation Modulates Gut Microbiota, Regulates ß-Catenin Expression and Prevents Weight Gain in ob/ob Mice: Preliminary Findings.

BACKGROUND: Obesity is one of the main health problems in the world today, and dysbiosis seems to be one of the factors involved. The aim of this study was to examine the impact of synbiotic supplementation on obesity and the microbiota in ob/ob mice. Twenty animals were divided into four groups: obese treated (OT), obese control (OC), lean treated (LT) and lean control (LC). All animals received a standard diet for 8 weeks. The treated groups received a synbiotic (Simbioflora-Invictus Farmanutrição Ltd., Sao Paulo, Brazil) in water, while the nontreated groups received only water. After 8 weeks, all animals were sacrificed, and gut tissue and stool samples were collected for mRNA isolation and microbiota analysis, respectively. ß-Catenin, occludin, cadherin and zonulin in the gut tissue were analyzed via RT-qPCR. Microbiome DNA was extracted from stool samples and sequenced using an Ion PGM Torrent platform. RESULTS: Synbiotic supplementation reduced body weight gain in the OT group compared with the OC group (p = 0.0398) and was associated with an increase in Enterobacteriaceae (p = 0.005) and a decrease in Cyanobacteria (p = 0.047), Clostridiaceae (p = 0.026), Turicibacterales (p = 0.005) and Coprococcus (p = 0.047). On the other hand, a significant reduction in Sutterella (p = 0.009) and Turicibacter (p = 0.005) bacteria was observed in the LT group compared to the LC group. Alpha and beta diversities were different among all treated groups. ß-Catenin gene expression was significantly decreased in the gut tissue of the OT group (p ≤ 0.0001) compared to the other groups. No changes were observed in occludin, cadherin or zonulin gene expression in the gut tissue. CONCLUSIONS: Synbiotic supplementation prevents excessive weight gain, modulates the gut microbiota, and reduces ß-catenin expression in ob/ob mice.

GENDER NARRATIVES IN SCHOOL PHYSICAL EDUCATION: SCOPING REVIEW OF BRAZILIAN SCIENTIFIC LITERATURE IN HEALTH SCIENCES / NARRATIVAS DE GÉNERO EN LA EDUCACIÓN FÍSICA ESCOLAR: Scoping Review de la literatura científica brasileña en Ciencias de la Salud / AS NARRATIVAS DE GÊNERO NA EDUCAÇÃO FÍSICA ESCOLAR: SCOPING REVIEW DA LITERATURA CIENTÍFICA BRASILEIRA NAS CIÊNCIAS DA SAÚDE

This study aims to understand which gender narratives are present in Brazilian scientific production in health sciences regarding school Physical Education. It is an exploratory research, with a qualitative approach. The methodology used was the scoping review and scientific articles found in the Virtual Health Library (Portal BVS) were included in this research. There were 16 articles, which were the sample of this study and were fully read. As a result, it can be stated that: the publications mostly involve public institutions and most of them happened in the decade of 2010; elementary school was the most present educational level and students had an important participation in these researches; the studies were mostly qualitative, with observation and the questionnaire being the most used instruments for data collection. Regarding the concept of gender, the analysis showed that all the works that dealt with these relationships understand gender as a social and historical construction. In this sense, it can be concluded that the Brazilian scientific production in the field of Health Sciences that focused on gender relations in school physical education was outlined by the search for a better understanding of an exclusive, sexist scenario, which stimulates in different ways the bodily experiences of boys and girls and that reproduces stereotypes related to femininities and masculinities and thus, throughout history, has placed girls on a lower and unequal level in school education.

El objetivo es comprender qué narrativas de género están presentes en la producción científica brasileña en ciencias de la salud con respecto a la Educación Física escolar. La metodología fue la revisión de alcance y se incluyeron en esta investigación 16 artículos científicos que se encuentran en la Biblioteca Virtual en Salud. Como resultado, se puede decir que: la mayoría de las publicaciones involucran a instituciones públicas, y la mayoría de ellas tuvieron lugar en la década de 2010; La Enseñanza Primaria fue el nivel educativo más presente y los estudiantes tuvieron una participación importante en estas encuestas; los trabajos fueron en su mayoría cualitativos, siendo la observación y el cuestionario los instrumentos de recolección más utilizados. En cuanto al concepto de género, el análisis mostró que todos los trabajos que abordaron estas relaciones entienden el género como una construcción social e histórica. En ese sentido, se puede concluir que la producción científica brasileña en el campo de las Ciencias de la Salud que se centró en las relaciones de género en la educación física escolar estuvo delineada por la búsqueda de una mejor comprensión de un escenario excluyente, sexista, que estimula de diversas formas la vivencias corporales de niños y niñas y que reproduce estereotipos relacionados con la feminidad y la masculinidad y así, a lo largo de la historia, ha situado a las niñas en un nivel inferior y desigual en la educación básica.

Este estudo tem como objetivo compreender quais as narrativas de gênero presentes na produção científica brasileira nas ciências da saúde no que diz respeito à Educação Física escolar. Trata-se de uma pesquisa exploratória, de abordagem qualitativa. A metodologia utilizada foi a scoping review e foram incluídos nesta pesquisa artigos científicos encontrados na Biblioteca Virtual em Saúde (BVS). Chegou-se a 16 artigos, os quais consistiram na amostra deste estudo e foram lidos na íntegra. Como resultado pode-se afirmar que: as publicações envolvem instituições públicas em sua maioria e, a maior parte deles, aconteceu na década de 2010; o Ensino Fundamental foi o nível educacional mais presente e os/as estudantes tiveram participação importante nessas pesquisas; os trabalhos se mostraram, majoritariamente, qualitativos, sendo a observação e o questionário os instrumentos de coleta mais utilizados. Em relação ao conceito de gênero, a análise evidenciou que todos os trabalhos que tratavam dessas relações entendem gênero como uma construção social e histórica. Nesse sentido, pode-se concluir que a produção científica brasileira no campo das Ciências da Saúde que se debruçou sobre as relações de gênero na educação física escolar esteve delineada pela busca de melhor compreensão de um cenário excludente, sexista, que estimula de maneiras diferentes as vivências corporais de meninos e meninas e que reproduz estereótipos relacionados às feminilidades e masculinidades e assim, ao longo da história, colocou as meninas num plano inferior e desigual na educação básica.

Recruitment methods and yield rates in a clinical trial of physical exercise for older adults with hypertension-HAEL Study: a study within a trial.

BACKGROUND: Although the prevalence of hypertension is high in older adults, clinical trial recruitment is a challenge. Our main aim was to describe the HAEL Study recruitment methods and yield rates. The secondary objectives were to explore the reasons for exclusion and to describe the characteristics of the enrolled participants. METHODS: This is a descriptive study within a trial. The HAEL Study was a Brazilian randomized two-center, parallel trial with an estimated sample of 184 participants. The recruitment strategy was based on four methods: electronic health records, word of mouth, print and electronic flyer, and press media. The yield rate was the ratio of the number of participants who underwent randomization to the total number of volunteers screened, calculated for overall, per recruitment method, by study center and by age group and sex. Additionally, we described the reasons for exclusion in the screening phase, as well as the demographic characteristics of those enrolled. The data are presented in absolute/relative frequencies and mean ± standard deviation. RESULTS: A total of 717 individuals were screened, and 168 were randomized over 32 months. The yield rate was higher for word of mouth (30.1%) in the overall sample. However, press media contributed the most (39.9%) to the absolute number of participants randomized in the trial. The coordinating center and participating center differed in methods with the highest yield ratios and absolute numbers of randomized participants. The main reason for exclusion in the screening phase was due to the physically active status in those intending to participate in the study (61.5%). Out of 220 participants included, 52 were excluded mainly because they did not meet the eligibility criteria (26.9%). Most of the screened volunteers were women (60.2%) age 60-69 years (59.5%), and most of the randomized participants were Caucasian/white (78.0%). CONCLUSIONS: Multiple recruitment methods constituted effective strategies. We observed that approximately one of every four individuals screened was allocated to an intervention group. Even so, there were limitations in obtaining a representative sample of older Brazilian adults with hypertension. Data show an underrepresentation of race and age groups. TRIAL REGISTRATION: This SWAT was not registered.

Cytomorphometric Evaluation of Oral Mucosa of Patients with Duchenne Muscular Dystrophy.

Duchenne muscular dystrophy (DMD) is a rare genetic disorder in which patients progressive muscle deterioration and low life expectancy. Since DMD has no cure, treatment slows the deterioration process using corticosteroids and other medications, including oral bisphosphonates (BP). These drugs can lead to toxicities in both soft and hard tissues. The objective of this study was to present cytological changes in DMD patients, comparing to young, healthy individuals. Cytological smears were obtained from buccal mucosa from nine DMD patients and five healthy patients. The Papanicolaou technique was used to stain the slides. Images were captured using a photomicroscope, and 50 clearly defined cells were selected. The size and ratio between the nucleus and cytoplasmic areas (NA/CA) diameter were measured using a computer program. All the DMD patients presented a statistical difference (p < 0.0001) in the size of the cytoplasm (0.60 ± 0.33 µm) and the NA/CA ratio (0.17 ± 0.07 µm) was seen when compared to the control group (cytoplasm 0.60 ± 0.15 µm and nucleus 0.14 ± 0.05 µm). Furthermore, in one DMD in use of BP who presented Medication-Related Osteonecrosis of the Jaw (MRONJ), presented higher differences in the cytoplasmic (1.20 ± 0.65 µm) and nuclear (0.20 ± 0.12 µm) sizes (p < 0.0001) compared with all others DMD patients. These results suggest that the drugs used or the systemic condition of individuals with DMD may contribute to these changes.

ATLANTIC ANTS: a data set of ants in Atlantic Forests of South America.

Ants, an ecologically successful and numerically dominant group of animals, play key ecological roles as soil engineers, predators, nutrient recyclers, and regulators of plant growth and reproduction in most terrestrial ecosystems. Further, ants are widely used as bioindicators of the ecological impact of land use. We gathered information of ant species in the Atlantic Forest of South America. The ATLANTIC ANTS data set, which is part of the ATLANTIC SERIES data papers, is a compilation of ant records from collections (18,713 records), unpublished data (29,651 records), and published sources (106,910 records; 1,059 references), including papers, theses, dissertations, and book chapters published from 1886 to 2020. In total, the data set contains 153,818 ant records from 7,636 study locations in the Atlantic Forest, representing 10 subfamilies, 99 genera, 1,114 ant species identified with updated taxonomic certainty, and 2,235 morphospecies codes. Our data set reflects the heterogeneity in ant records, which include ants sampled at the beginning of the taxonomic history of myrmecology (the 19th and 20th centuries) and more recent ant surveys designed to address specific questions in ecology and biology. The data set can be used by researchers to develop strategies to deal with different macroecological and region-wide questions, focusing on assemblages, species occurrences, and distribution patterns. Furthermore, the data can be used to assess the consequences of changes in land use in the Atlantic Forest on different ecological processes. No copyright restrictions apply to the use of this data set, but we request that authors cite this data paper when using these data in publications or teaching events.

Infection Transmission Associated With Contaminated Ultrasound Probes: A Systematic Review.

A systematic review of seven studies on infections related to contaminated ultrasound probes showed that the infections were related to a failure in the decontamination process of ultrasound devices used on immature skin of neonates in an intensive care unit and transesophageal echocardiography probes. Six of the studies involved outbreaks in patients who underwent transesophageal echocardiography either during or after surgery or as a part of treatment for a nonsurgical cardiac condition. The evidence shows links between the infection outbreaks and environmental contamination, lack of standardized ultrasound probe disinfection processes, inadequate storage, and lack of monitoring of probe integrity. When personnel addressed the deficiencies (eg, improving the disinfection process, cleaning the probes immediately after use, inspecting the probes for defects), the infections ceased. Personnel involved with the reprocessing of ultrasound probes should clean, disinfect, inspect, and store ultrasound probes in a manner that maintains device integrity and prevents contamination.

Da adolescência atual ao atual da adolescência / From contemporary adolescence to the actual of adolescence / De l'adolescence actuelle à l'actuel de l'adolescence / De la adolescencia actual a lo actual de la adolescencia

Este artigo origina-se da inquietação dos autores diante dos debates em torno da adolescência atual. Partindo de três filmes lançados em 2016 (Raw, Demônio de neon e Mate-me por favor), propomos um debate sobre o atual da adolescência como aquilo que não cessa de não se escrever. Em termos metodológicos, consideramos os momentos em que assistimos aos filmes como um sonho recorrente e seus relatos como uma "elaboração secundária", onde rearranjamos seus significantes. Observamos, assim, alguns temas transversais: um jogo escópico frequentemente aprisionado em uma dimensão especular, canibalismo e a presença de um gozo erótico mórbido. Por fim, enlaçamos esses elementos com o intuito de formular uma pergunta: a proliferação de discursos sobre a adolescência consistiria em um esforço, sempre fracassado, de nomear o inominável?

This article concerns the debates about contemporary adolescence. Based on three feature films released in 2016 (Raw, The Neon Demon and Kill me please), it discusses the actual of adolescence, understood as that which does not stop not writing itself. Methodology involves considering the moments when we watched the movies as a recurring dream and their narratives as a "secondary elaboration", rearranging their signifiers. As a result, some transversal themes were observed: the capture of the subject in his own specular image, cannibalism and morbid-erotic jouissance. Finally, we connect these elements to formulate the following question: would the proliferation of discourses about adolescence be an ever-failed effort to name something that cannot be named?

Cet article découle de l'inquiétude des auteurs face aux débats autour de l'adolescence actuelle. À partir de trois films sortis en 2016 (Grave, The Neon Demon et Tue-moi, s'il te plaît), nous proposons un débat sur l'actuel de l'adolescence comme ce qui ne cesse pas de ne pas s'écrire. En termes méthodologiques, nous considérons les moments où nous regardons les films comme un rêve récurrent et leurs narratives comme une "élaboration secondaire", ce qui nous permet de réorganiser leurs signifiants. Nous observons ainsi quelques thèmes transversaux: un jeu scopique souvent piégé dans une dimension spéculaire, le cannibalisme et la présence d'une jouissance érotique morbide. Enfin, nous lions ces éléments pour poser une question: la prolifération des discours sur l'adolescence consisterait-elle en une tentative toujours vaine de nommer l'innommé?

Este artículo surge de la inquietud de los autores frente a los debates relacionados a la adolescencia actual. A partir de tres películas lanzadas en 2016 (Voraz, El demonio neón y Mátame por favor), proponemos un debate sobre lo actual de la adolescencia como aquello que no deja de no escribirse. En términos metodológicos, consideramos los momentos en que vemos las películas como un sueño recurrente y sus relatos como una "elaboración secundaria", donde reemplazamos sus significantes. De este modo, observamos algunos temas transversales: un juego escópico a menudo atrapado en una dimensión especular, canibalismo y presencia de un goce erótico mórbido. Finalmente, enlazamos estos elementos para hacer una pregunta: ¿La proliferación de discursos sobre la adolescencia consistiría en un intento,siempre fallido, de nombrar lo innombrable?

Climatic factors affecting gestational length in mares under subtropical climate.

The aim of this study was (i) to evaluate the relationship of climatic factors with gestational length (GL) and (ii) to evaluate the relationship of sire, foal gender and maternal factors with GL in mares. Retrospective data from 470 gestations of 202 respective mares were collected from a Criollo breeding farm in the southern hemisphere. GL was considered as the interval between ovulation and parturition. Climatic and environmental data (temperature, relative humidity, daily sunshine hours, precipitation, temperature humidity index - THI) were obtained daily and the mean values for foaling month and season were calculated. A multiple factor analysis of variance was performed to determine the relationship of the independent variables and interactions related to the mare, foal gender and stallion, and those related to the weather in each season of the year with GL as the dependent variable. The mean ± standard error (SE) of GL for the 470 gestations was 337 ± 0.4 days with a range of 311-363 days. Young-primiparous mares (340±0.9) had longer gestations (P < .001) than young-multiparous (336 ± 0.7) and mature-multiparous (334 ± 0.7) mares. Foal gender had no effect on GL. Gestational length was found to be affected by the stallion. Mares which experienced an autumn and winter gestational period with less daily sunshine hours had an increased GL (P < .05). In conclusion, environmental and climatic factors during pregnancy influenced the GL in mares, specifically, daily sunshine hours, precipitation and THI presented a negative correlation with GL. Young-primiparous Criollo mares presented longer gestational lengths than young or mature multiparous mares.

Operational and biochemical aspects of co-digestion (co-AD) from sugarcane vinasse, filter cake, and deacetylation liquor.

This work performed co-AD from the vinasse and filter cake (from 1G ethanol production) and deacetylation liquor (from the pretreatment of sugarcane straw for 2G ethanol production) in a semi-Continuous Stirred Tank Reactor (s-CSTR) aiming to provide optimum operational parameters for continuous CH4 production. Using filter cake as co-substrate may allow the reactor to operate throughout the year, as it is available in the sugarcane off-season, unlike vinasse. A comparison was made from the microbial community of the seed sludge and the reactor sludge when CH4 production stabilized. Lactate, butyrate, and propionate fermentation routes were denoted at the start-up of the s-CSTR, characterizing the acidogenic phase: the oxidation-reduction potential (ORP) values ranged from -800 to -100 mV. Once the methanogenesis was initiated, alkalizing addition was no longer needed as its demand by the microorganisms was supplied by the alkali characteristics of the deacetylation liquor. The gradual increase of the applied organic load rates (OLR) allowed stabilization of the methanogenesis from 3.20 gVS L-1 day-1: the highest CH4 yield (230 mLNCH4 g-1VS) and average organic matter removal efficiency (83% ± 13) was achieved at ORL of 4.16 gVS L-1 day-1. The microbial community changed along with the reactor operation, presenting different metabolic routes mainly due to the used lignocellulosic substrates. Bacteria from the syntrophic acetate oxidation (SAO) process coupled to hydrogenotrophic methanogenesis were predominant (~ 90% Methanoculleus) during the CH4 production stability. The overall results are useful as preliminary drivers in terms of visualizing the co-AD process in a sugarcane biorefinery integrated to scale. KEY POINTS: • Integration of 1G2G sugarcane ethanol biorefinery from co-digestion of its residues. • Biogas production from vinasse, filter cake, and deacetylation liquor in a semi-CSTR. • Lignocellulosic substrates affected the biochemical routes and microbial community. • Biomol confirmed the establishment of the thermophilic community from mesophilic sludge.

Immune response of adult horses, pregnant mares and foals to an experimental vaccine with recombinant EMA-2 protein of Theileria equi.

Equine theileriosis, caused by the Theileria equi protozoan, is a disease of worldwide importance. T. equi expresses surface proteins, of which the EMA-2 protein is a promising antigen for vaccine use. The aim of this study was to evaluate the immune response of adult horses, pregnant mares, and foals to an experimental EMA-2 protein of recombinant T. equi vaccine. A total of 46 horses were used in this study for vaccine trials and challenges. Twelve geldings, 14 pregnant mares, and 14 foals were divided into vaccinated and control groups. Total serum specific anti-rEMA-2 IgG, IgG subclasses, and transcription of cytokines related to the immune response were evaluated. For the vaccine challenge, six six-month-old foals were divided into vaccinated and control groups. For the challenge, blood from a horse with theileriosis was transfused to the foals. Geldings and pregnant mares maintained anti-rEMA-2 IgG levels at 130 and 140 days after vaccination, respectively. The most-detected IgG subclasses in vaccinated were IgG3/5, IgG4/7, and IgG1. IL2, IL10, IL12, IL17, IFN-γ, and TNF-α were the most-transcribed cytokines in PBMCs of vaccinated horses stimulated with rEMA-2. Challenge with T. equi demonstrated that vaccinated foals had an increase of 33% in total IgG four days after blood transfusion, while control foals had no significant response, suggesting that vaccine antibodies may have recognized EMA-2 protein of the native T. equi antigen. T. equi recombinant EMA-2 was shown to be a promising vaccine antigen by inducing humoral and cellular immunity similar to that observed in natural parasite infections.

SARS-CoV-2 aerosol generation during respiratory equipment reprocessing.

Aerosolization may occur during reprocessing of medical devices. With the current coronavirus disease 2019 pandemic, it is important to understand the necessity of using respirators in the cleaning area of the sterile processing department. To evaluate the presence of severe acute respiratory syndrome coronavirus (SARS-CoV-2) in the air of the sterile processing department during the reprocessing of contaminated medical devices. Air and surface samples were collected from the sterile processing department of two teaching tertiary hospitals during the reprocessing of respiratory equipment used in patients diagnosed with coronavirus disease 2019 and from intensive care units during treatment of these patients. SARS-CoV-2 was detected only in 1 air sample before the beginning of decontamination process. Viable severe acute respiratory syndrome coronavirus 2 RNA was not detected in any sample collected from around symptomatic patients or in sterile processing department samples. The cleaning of respiratory equipment does not cause aerosolization of SARS-CoV-2. We believe that the use of medical masks is sufficient while reprocessing medical devices during the coronavirus disease 2019 pandemic.