Microbiological Diversity and Associated Enzymatic Activities in Honey and Pollen from Stingless Bees from Northern Argentina.

Honey and pollen from Tetragonisca fiebrigi and Scaptotrigona jujuyensis, stingless bees from northern Argentina, presented a particular microbiological profile and associated enzymatic activities. The cultured bacteria were mostly Bacillus spp. (44%) and Escherichia spp. (31%). The phylogenetic analysis showed a taxonomic distribution according to the type of bee that was similar in both species. Microbial enzymatic activities were studied using hierarchical clustering. Bacillus spp. was the main bacterium responsible for enzyme production. Isolates with xylanolytic activity mostly presented cellulolytic activity and, in fewer cases, lipolytic activity. Amylolytic activity was associated with proteolytic activity. None of the isolated strains produced multiple hydrolytic enzymes in substantial amounts, and bacteria were classified according to their primary hydrolytic activity. These findings add to the limited knowledge of microbiological diversity in honey and pollen from stingless bees and also provide a physiological perspective of this community to assess its biotechnological potential in the food industry.

Comparative oil extraction from mutt (Myliobatis goodei) liver by enzymatic hydrolysis: free versus immobilized biocatalyst.

BACKGROUND: The development and fine-tuning of biotechnological processes for fish oil extraction constitute a very important focus to contribute to the development of a food industry based on fish consumption. This work lies in a comparative analysis of the oil extraction yield of Myliobatis goodei livers using free and immobilized enzymes. RESULTS: An immobilized biocatalyst was designed from the cell-free extract of a Bacillus sp. Mcn4. A complete factorial design was used to study the components of the bacterial culture medium and select the condition with the highest titers of extracellular enzymatic activities. Wheat bran had a significant effect on the culture medium composition for enzymatic production. The immobilized biocatalyst was designed by covalent binding of the proteins present in the cocktail retaining a percentage of different types of enzymatic activities (Mult.Enz@MgFe2 O4 ). Among the biocatalyst used, Alcalase® 2.4 L and Purazyme® AS 60 L (free commercial proteases) showed extraction yields of 87.39% and 84.25%, respectively, while Mult.Enz@MgFe2 O4 achieved a better one of 89.97%. The oils obtained did not show significant differences in their physical-chemical properties while regarding the fatty acid content, the oil extracted with Purazyme® AS 60 L showed a comparatively lower proportion of polyunsaturated fatty acids. CONCLUSIONS: Our results suggest that the use of by-products of M. goodei is a valid alternative and encourages the use of immobilized multienzyme biocatalysts for the treatment of complex substrates in the fishing industry. © 2023 Society of Chemical Industry.

Physical activity levels in various university majors amidst the COVID-19 pandemic: a comparative study / Nivel de actividad física en varias carreras universitarias en medio de la pandemia de COVID-19: Un estudio comparativo / Níveis de atividade física em várias carreiras universitárias em meio à pandemia de COVID-19: Um estudo comparativo

This study determined levels of physical activity in students by comparing them based on gender, faculty, and major among university students during the Covid-19 pandemic. The research followed a quantitative approach with a descriptive-comparative design. The study was conducted once per student, with the participation of 582 students of both genders. The International Physical Activity Questionnaire (IPAQ) was administered to the students using Google Forms, distributed through their institutional emails. The collected data were analyzed using the statistical software SPSS V.22.0. The independent samples t-test was employed to compare the energy expenditure between males and females, along with Cohen's d statistic to assess the effect size. Prior to these analyses, the Kolmogorov-Smirnov normality test and Levene's test were conducted. Results were considered significant when the p-value was <0.05. The findings indicate that males allocate more time to work than females. Additionally, it was observed that males exhibit a higher level of physical activity than females within the engineering field. Lastly, majors with the highest levels of physical activity per week were Physical Education and Nutrition. These outcomes shed light on the reality of physical activity levels among Chilean university students based on faculty and major. University authorities should consider promoting physical activity programs, particularly emphasizing women and majors such as engineering, architecture, and mathematics, which have shown lower levels of physical activity.

Este estudio determinó los niveles de actividad física en estudiantes comparando por sexo, facultad y carrera en estudiantes universitarios en tiempos de pandemia por de Covid-19. Este estudio tiene un enfoque de investigación cuantitativa con diseño descriptivo-comparativo. Este estudio se aplicó una sola vez por estudiante contando con la participación de 582 estudiantes de ambos sexos. A los estudiantes se les aplicó el cuestionario Internacional de Actividad Física (IPAQ) mediante Google forms haciéndoselos llegar sus correos institucionales. Los datos obtenidos se analizaron en el programa estadístico SPSS V.22.0. Se utilizo la prueba estadística T-student para muestras independientes para comparar el coste energético entre hombres y mujeres, además del estadístico d de Cohen para evaluar el tamaño del efecto. Antes de realizar estas evaluaciones se realizó la prueba de normalidad Kolmogorov Smirnov y prueba de Levene. Se consideraron resultados significativos cuando el valor de p fue <0.05. Los resultados indican que los hombres destinan mayor tiempo a trabajar que las mujeres, además se encontró que los hombres poseen mayor nivel de actividad física que las mujeres en ingeniería. Finalmente, los estudiantes de las carreras con mayor nivel de actividad física a la semana fueron Educación física y nutrición. Estos resultados dan cuenta de la realidad en universitarios chilenos respecto al nivel de actividad física por facultad y por carrera. Las autoridades universitarias deberían promover programas de actividad física enfatizando en mujeres y en carreras de ingeniería, arquitectura y matemática que han mostrado menores niveles de actividad física.

Este estudo determinou os níveis de atividade física em estudantes, comparando por gênero, faculdade e curso entre estudantes universitários durante a pandemia de Covid-19. A pesquisa seguiu uma abordagem quantitativa com um design descritivo-comparativo. O estudo foi conduzido uma única vez por estudante, com a participação de 582 estudantes de ambos os gêneros. O Questionário Internacional de Atividade Física (IPAQ) foi administrado aos estudantes por meio do Google Forms, distribuído por meio de seus e-mails institucionais. Os dados coletados foram analisados usando o software estatístico SPSS V.22.0. O teste t de amostras independentes foi empregado para comparar o gasto energético entre homens e mulheres, juntamente com a estatística d de Cohen para avaliar o tamanho do efeito. Antes dessas análises, o teste de normalidade de Kolmogorov-Smirnov e o teste de Levene foram conduzidos. Resultados foram considerados significantes quando o valor de p foi <0.05. Os resultados indicam que os homens dedicam mais tempo ao trabalho do que as mulheres. Adicionalmente, observou-se que os homens apresentam um nível mais elevado de atividade física do que as mulheres no campo da engenharia. Por fim, os cursos com os níveis mais altos de atividade física por semana foram Educação Física e Nutrição. Estes resultados lançam luz sobre a realidade dos níveis de atividade física entre estudantes universitários chilenos com base na faculdade e no curso. As autoridades universitárias devem considerar a promoção de programas de atividade física, especialmente enfatizando as mulheres e os cursos como engenharia, arquitetura e matemática, que mostraram níveis mais baixos de atividade física.

Interfacial Hyperactivation of Candida rugosa Lipase onto Ca2Fe2O5 Nanoparticles: pH and Ionic Strength Fine-Tuning to Modulate Protein-Support Interactions.

The current study provides a comprehensive look of the adsorption process of Candida rugosa lipase (CRL) on Ca2Fe2O5 iron oxide nanoparticles (NPs). Protein-support interactions were identified across a broad range of pH and ionic strengths (mM) through a response surface methodology, surface charge determination, and spectroscopic and in silico analyses. The maximum quantity of immobilized protein was achieved at an ionic strength of 50 mM and pH 4. However, this condition did not allow for the greatest hydrolytic activity to be obtained. Indeed, it was recorded at acidic pH, but at 150 mM, where evaluation of the recovered activity revealed hyperactivation of the enzyme. These findings were supported by adsorption isotherms performed under different conditions. Based on zeta potential measurements, electrostatic interactions contributed differently to protein-support binding under the conditions tested, showing a strong correlation with experimentally determined immobilization parameters. Raman spectra revealed an increase in hydrophobicity around tryptophan residues, whereas the enzyme immobilization significantly reduced the phenylalanine signal in CRL. This suggests that this residue was involved in the interaction with Ca2Fe2O2 and molecular docking analysis confirmed these findings. Fluorescence spectroscopy showed distinct behaviors in the CRL emission patterns with the addition of Ca2Fe2O5 at pH 4 and 7. The calculated thermodynamic parameters indicated that the contact would be mediated by hydrophobic interactions at both pHs, as well as by ionic ones at pH 4. In this approach, this work adds to our understanding of the design of biocatalysts immobilized in iron oxide NPs.

Vernalization Requirement, but Not Post-Vernalization Day Length, Conditions Flowering in Carrot (Daucus carota L.).

Carrots require a certain number of cold hours to become vernalized and proceed to the reproductive stage, and this phenomenon is genotype-dependent. Annual carrots require less cold than biennials to flower; however, quantitative variation within annuals and biennials also exists, defining a gradient for vernalization requirement (VR). The flowering response of carrots to day length, after vernalization has occurred, is controversial. This vegetable has been described both as a long-day and a neutral-day species. The objective of this study was to evaluate flowering time and frequency in response to different cold treatments and photoperiod regimes in various carrot genotypes. To this end, three annual genotypes from India, Brazil, and Pakistan, and a biennial carrot from Japan, were exposed to 7.5 °C during 30, 60, 90, or 120 days, and then transferred to either long day (LD) or short day (SD) conditions. Significant variation (p < 0.05) among the carrot genotypes and among cold treatments were found, with increased flowering rates and earlier onset of flowering being associated with longer cold exposures. No significant differences in response to photoperiod were found, suggesting that post-vernalization day length does not influence carrot flowering. These findings will likely impact carrot breeding and production of both root and seed, helping in the selection of adequate genotypes and sowing dates to manage cold exposure and day-length for different production purposes.

Hybridization and polyploidization effects on LTR-retrotransposon activation in potato genome.

Hybridization and polyploidization are major forces in plant evolution and potatoes are not an exception. It is proposed that the proliferation of Long Terminal Repeat-retrotransposons (LTR-RT) is related to genome reorganization caused by hybridization and/or polyploidization. The main purpose of the present work was to evaluate the effect of interspecific hybridization and polyploidization on the activation of LTR-RT. We evaluated the proliferation of putative active LTR-RT in a diploid hybrid between the cultivated potato Solanum tuberosum and the wild diploid potato species S. kurtzianum, allotetraploid lines derived from this interspecific hybrid and S. kurtzianum autotetraploid lines (ktz-autotetraploid) using the S-SAP (sequence-specific amplified polymorphism) technique and normalized copy number determination by qPCR. Twenty-nine LTR-RT copies were activated in the hybrid and present in the allotetraploid lines. Major LTR-RT activity was detected in Copia-27, Copia-12, Copia-14 and, Gypsy-22. According to our results, LTR-RT copies were activated principally in the hybrid, there was no activation in allotetraploid lines and only one copy was activated in the autotetraploid.

Giant Congenital Melanocytic Nevus in a Pediatric Patient: Case Report.

The giant congenital melanocytic nevus is a rare entity with an extremely low incidence that appears at the time of birth and generally involves the dermis but may also affect other skin layers. According to its clinical evolution, the probability of malignancy may vary, so proper follow-up is essential for potential management. There is no consensus in the literature about the greater benefit of surgical versus nonsurgical management. In this case report, we present the surgical management of a school-aged patient using dermal substitutes and skin grafts, subjectively obtaining an improvement in his quality of life.

Multimodal Early Alzheimer's Detection, a Genetic Algorithm Approach with Support Vector Machines.

Alzheimer's disease (AD) is a neurodegenerative disease that mainly affects older adults. Currently, AD is associated with certain hypometabolic biomarkers, beta-amyloid peptides, hyperphosphorylated tau protein, and changes in brain morphology. Accurate diagnosis of AD, as well as mild cognitive impairment (MCI) (prodromal stage of AD), is essential for early care of the disease. As a result, machine learning techniques have been used in recent years for the diagnosis of AD. In this research, we propose a novel methodology to generate a multivariate model that combines different types of features for the detection of AD. In order to obtain a robust biomarker, ADNI baseline data, clinical and neuropsychological assessments (1024 features) of 106 patients were used. The data were normalized, and a genetic algorithm was implemented for the selection of the most significant features. Subsequently, for the development and validation of the multivariate classification model, a support vector machine model was created, and a five-fold cross-validation with an AUC of 87.63% was used to measure model performance. Lastly, an independent blind test of our final model, using 20 patients not considered during the model construction, yielded an AUC of 100%.

Addressing Sustainable Social Change for All: Upcycled-Based Social Creative Businesses for the Transformation of Socio-Technical Regimes.

The global challenges caused by socio-economic inequalities, climate change and environmental damage caused to ecosystems, require changes in human behavior at all organizational levels, including companies, governments, communities, and individuals. In this context, it is important to analyse how social and creative companies that work in the fashion and industrial design recycling sector can address sustainable social change. In this paper, we propose an analysis in the countries of the global South. To learn how grassroots innovations can contribute to the development of sustainable strategies, we perform the framework of Technical transitions. We analyze the three main areas of activity that constitute an effective niche construction: social networks, expectations and visions, and learning. A qualitative methodology is used, a video case study with six grassroots organizations in South Africa, Sri Lanka, Malaysia, and Brazil. The results reflect the important role played by these grassroots innovations, contributing to the development of social and creative recycling companies that address socio-economic and environmental problems.

Remodelación de papila gingival interdental con ácido hialurónico. Una solución estética / Remodeling interdental gingival papilla with hyaluronic acid. An aesthetic solution

RESUMEN: El objetivo de este trabajo es evaluar el efecto de remodelación y relleno mediante aplicación de ácido hialurónico en papilas gingivales de sector estético con defectos de triángulo negro en un paciente tratado periodontalmente. Se inyectaron 0.15 ml de gel de ácido hialurónico dividido en 3 sesiones. Clínicamente se observó aumento de volumen y aumento de dimensiones verticales y horizontales en las papilas, mejorando así los parámetros estéticos. Como conclusión la aplicación de ácido hialurónico es efectiva en la remodelación y relleno en pérdidas de papila clase I de Nordland y Tarnow.

ABSTRACT: The objective of this work is to evaluate the effect of remodeling and filling through the application of hyaluronic acid in gingival papilla in the aesthetic sector with black triangle defects, in a periodontally- treated patient. In 3 portioned sessions, 0.15 ml of hyaluronic acid gel was injected. Clinically, an increase in volume was observed, and vertical and horizontal dimensions in the papilla increased too, thus improving aesthetic parameters. In conclusion, the application of hyaluronic acid is effective in the remodeling and filling in losses of class I papilla (Nordland and Tarnow classification).

Frequency and tendency of malaria in Colombia, 1990 to 2011: a descriptive study.

BACKGROUND: Malaria is a serious health problem in Colombia. This paper intends to analyse the frequency and tendencies of the disease in Colombia over the last 22 years. The researchers used the Box-Jenkins (ARIMA) methodology for the analysis of time series. METHODS: This descriptive study was done retrospectively by using the morbidity records of the Ministry of Health and of the System for the Monitoring of Public Health (SIVIGILA). The information about the population was obtained from the National Administrative Department of Statistics (DANE). The incidence rate according to age and sex was calculated from 1990 to 2011. Also, the Annual Parasite Index (API) for Plasmodium falciparum and for Plasmodium vivax was calculated. The mortality rates per year, from 1990 to 2011, were determined. Finally, the Box-Jenkins (ARIMA) methodology was used for the analysis of time series, grouped weekly. Information for ARIMA modelling was used from the year 2001. RESULTS: The total number of reported cases from 1990 to 2011 was 2,964,818 cases with an annual average of 134,764. In the period from 2001 to 2005 and from 2006 to 2011 a significant decrease of annual cases was observed. In general, a predominance of P. vivax over P. falciparum was observed. With respect to the API, it must be noted that there were peaks in 1994 in the departments of Guainía and Guaviare, and in 1998 in Guaviare and Chocó. The department of Antioquia showed a tendency towards a decrease of the API through the years.In the time series model there were no statistically significant seasonal patterns for the total number of cases of malaria. However, for P. falciparum the number of cases was statistically significant. Lastly, between 1990 and 2009, there were 1,905 deaths caused by malaria in Colombia with a significant tendency towards a decrease in deaths over those years. Plasmodium falciparum was more lethal than P. vivax. CONCLUSIONS: In Colombia, the transmission of malaria occurs in an endemic and epidemic context, which keeps an unstable endemic transmission pattern. Several factors specific to a country such as Colombia encourage the dissemination and permanence of the illness.

Distribución y abundancia de piojos (Werneckiella equi) y eficacia del tratamiento con Triclorfón en caballos mestizos / Distribution and Abundance of Lice (Werneckiella equi) and Efficacy of Treatment Using Trichlorfon in Crossbred Horses

El piojo masticador Werneckiella equi es un ectoparásito comúnmente encontrado en equinos; puede causar irritación, llevando a hiperqueratosis, prurito intenso y alopecia. Existen diversos productos en el mercado utilizados para el tratamiento de ectoparásitos; sin embargo, la información disponible sobre su efectividad es escasa. El objetivo de este estudio fue determinar la abundancia y distribución de W. equi y la eficacia de triclorfón en el control de los phthirapteras en caballos mestizos, utilizando una dosis única, aplicada mediante aspersión. Se utilizaron 34 caballos mestizos naturalmente infestados con piojos, que se distribuyeron al azar en dos grupos: Control (sin tratamiento; n=17) y Tratado con triclorfón diluido al 0,15% (n=17). La distribución y abundancia de los piojos fue estimada por medio de recuento directo de los phthirapteras en diferentes zonas del cuerpo del caballo, observándose solo diferencias significativas (P≤0,05) en la región de la mano con respecto a zona del abdomen y dorso lateral del tronco. La efectividad fue evaluada a los 28 días posttratamiento, observándose diferencias significativas entre el grupo Control y Tratado, resultando en un porcentaje de eficacia del 93,5% y demostrándose que triclorfón aplicado al 0,15% muestra un efectivo tratamiento para piojos en equinos.

The chewing louse, Werneckiella equi, is an ectoparasite commonly found in equine. This parasite may cause irritation, leading to hyperkeratosis, intense pruritus, and alopecia. There are several products in the market used in the treatment of ectoparasites; however, available information regarding their effectiveness is scarce. A study was undertaken to determine both the abundance and distribution of W. equi and the efficacy of trichlorfon (an organophosphate) in controlling the horse lice, using a single dose, employing the spray method. A total of 34 crossbred horses, naturally infested with lice were used. The animals were randomly distributed into two groups: Control group (without treatment; n=17); and Treatment group (n=17): animals treated with 0.15% w/v trichlorfon. The distribution and abundance of lice were determined by direct counting of phthiraptera in different areas of the horse body. The results show only minor significant differences (P≤0.05) in the hand region, when compared to the abdomen and the dorso-lateral trunk. The efficacy was evaluated 28 d posttreatment, showing significant differences between the two groups, being the treatment efficacy of 93.5%. It is concluded that trichlorfon, when applied as a 0.15% w/v spray, results in an effective treatment for equine lice.

Evaluación de competencias en proceso enfermero durante el servicio social / Competency assessment in nursing process during social service

Introducción: El proceso enfermero es la herramienta que sistematiza las intervenciones de enfermería en los pacientes de una forma racional, lógica y sustantiva, además de contribuir a mejorar la calidad de la atención. Objetivo: Evaluar las áreas de competencia disciplinar, cognitiva, actitudinal y procedimental de pasantes de licenciatura en enfermería en el proceso enfermero aplicado durante el servicio social. Metodología: Se evaluaron con 24 reactivos a 97 pasantes de licenciatura en enfermería de la Escuela Superior de Enfermería y Obstetricia del Instituto Politécnico Nacional, que realizaron servicio social en hospitales de la Secretaría de Salud. Se realizó análisis simple y de correlación. Resultados: 73.2 % de los pasantes consideró tener buen nivel de conocimientos sobre el proceso enfermero, en el componente procedimental obtuvieron calificación promedio de 9.0 y en el actitudinal 51.5 % refirió que el proceso enfermero mejora la calidad de atención, 8.2 % lo señaló como una fortaleza, 2.1 % no concibe a la disciplina sin un método organizado de trabajo y 1 % lo consideró como un criterio para la certificación de enfermería. Conclusiones: El proceso enfermero es el método de trabajo fundamental para brindar mejor atención, distinguiendo a la enfermera de otros profesionales de la salud por su visión integral, que le permite permear la esfera biológica, psicológica y social del paciente, familia y comunidad. Las escuelas de enfermería son el medio para que los alumnos adquieran conocimientos, destrezas y habilidades en el proceso enfermero que les permitirá ser profesionales calificados.

Introduction: The nursing process is the tool that systematizes nursing interventions toward the patients in a rational, logical, and substantive way and contributes to improve the quality of Service. Objective: To evaluate the areas of discipline competency in abilities on knowledge, attitude, and procedures on baccalaureate graduated nursing students on the nursing process applied during the social Service. Methodology: 24 items were assessed on 97 baccalaureate graduated nursing students of Superior Nursing and Obstetric School of the Instituto Politécnico Nacional, who carried out social Service in Health Department hospitals. Simple and correlation analysis were done. Results: 73.2 % graduates considered they have good level of know-ledge about the nursing process; on the component that assessed procedures, they obtained an average score of 9.0; and in the attitudinal area, 51.5 % referred that nursing process improves the quality of Service; 8.2 % pointed it out as a strength, 2.1 % does not conceive the discipline without an organized method of work, and 1 % considered it as a criterion for the nursing certification. Conclusions: The nursing process is the method of fundamental work to give a better attention distinguishing the nurse from other allied health professionals because of their integral vision that allows permeating the biological, psychological, and social spheres of patient, family and community. Nursing schools are the means for students to acquire knowledge, skills, and abilities in the nursing process which will allow them to be qualified professionals. The nursing schools are the students medium to acquire the knowledge, dexterities and abilities in the nurse process that permit them to be qualified professionals.

Niveles de antioxidantes como parametro de actividad inflamatoria en la artritis reumatoidea / Antioxidants levels and rheumatoid arthritis

La artritis reumatoidea (AR) es una enfermedad sistémica caracterizada por la inflamación crónica y simétrica de las articulaciones. Los radicales libres son moléculas que podrían intervenir en el proceso inflamatorio articular y sistémico. El sistema antioxidante es un conjunto de sustancias que impiden la formación de radicales libres. Objetivo: determinar la actividad de dos sustancias antioxidantes, la glutatión peroxidasa (GpX) y la superóxido dismutasa (SOD) en pacientes con AR y en controles sanos. Material y métodos: se incluyeron 60 individuos de ambos sexos entre 30 y 60 años, divididos en tres grupos de 20 personas, uno constituido por pacientes con AR activa, otro por pacientes con AR en remisión (según los criterios de remisión de Pinals) y el tercero por controles sanos apareados por sexo y edad con el grupo de pacientes con AR activa. En los dos grupos de pacientes con AR se realizó un recuento de articulaciones inflamadas utilizando el índice articular de Thompson (0= ninguna articulación inflamada; 534= puntaje máximo de inflamación). Se midieron la proteína C reactiva (POR) y la velocidad de sedimentación globular (VSG) en todos los sujetos del estudio. Se realizó la medición en sangre total de SOD (rango normal =164-240 U/ml) y de GpX (rango normal = 4170-10881 U/L) en los tres grupos. Método estadístico: se calcularon los valores p de la prueba t de diferencias de medias poblacionales entre los tres grupos para la SOD, la GpX, la PCR y la VSG. Resultados: el puntaje promedio del índice de Thompson en los pacientes con AR activa fue de 177.5 y en el grupo en remisión fue de 5.5. El nivel promedio de GpX en los individuos sanos (X=6991.5 U/L) y en los pacientes con AR en remisión (X=5703.4 U/L) se encontró dentro de los valores de referencia. En el grupo de AR activa se encontró disminuido de manera significativa (X=2847 U/L) si se compara con el grupo de AR en remisión (p=0.008) y con el de controles sanos (p=4.7 x 105). Para la SOD se encontraron resultados similares en el grupo de sanos (X=212.5 U/ml) y el grupo de pacientes en remisión (205.6 U/ml...

Amputación interescapulotorácica por cromomicosis y carcinoma epidermoide / Interscapulothoracic amputation by chromomycosis and epidermoid carcinoma

Paciente del sexo masculino y blanco de 74 años de edad, con lesión dermatológica hiperpigmentada y verrucosa de más de 25 años de evolución en codo y antebrazo izquierdo; asimismo posee otra de piel en forma de coliflor y cuya evolución es reciente. Ambas presentaron diagnóstico histopatológico de cromomicosis. El tratamiento inicial fue la exéresis con margen oncológico de la lesión en forma de coliflor y la electrofulguración, curetaje del resto de la lesión y tratamiento antimicótico. En un período de 5 meses el enfermo presenta evolución tórpida con toma del estado general y elefantiasis del miembro superior izquierdo hasta región supraclavicular que obliga a realizarle amputación interescapulotorácica por la técnica de Berger para mejorar la calidad de vida. El diagnóstico histopatológico de los paquetes ganglionares resecados mostró metástasis de un carcinoma epidermoide(AU)

Amputación interescapulotorácica por cromomicosis y carcinoma epidermoide / Interscapulothoracic amputation by chromomycosis and epidermoid carcinoma

Paciente del sexo masculino y blanco de 74 años de edad, con lesión dermatológica hiperpigmentada y verrucosa de más de 25 años de evolución en codo y antebrazo izquierdo; asimismo posee otra de piel en forma de coliflor y cuya evolución es reciente. Ambas presentaron diagnóstico histopatológico de cromomicosis. El tratamiento inicial fue la exéresis con margen oncológico de la lesión en forma de coliflor y la electrofulguración, curetaje del resto de la lesión y tratamiento antimicótico. En un período de 5 meses el enfermo presenta evolución tórpida con toma del estado general y elefantiasis del miembro superior izquierdo hasta región supraclavicular que obliga a realizarle amputación interescapulotorácica por la técnica de Berger para mejorar la calidad de vida. El diagnóstico histopatológico de los paquetes ganglionares resecados mostró metástasis de un carcinoma epidermoide

La citología de impresión conjuntival y la biopsia de la glándula salival accesoria en el síndrome de ojo seco / Conjunctival impression cytology and biopsy of the accesory salival gland in the syndrome of the dry eye

Se realizó una investigación en 7 pacientes con el síndrome de ojo seco, cuyas edades estaban comprendidas entre los 24 y 60 años, con un sólo integrante del sexo masculino y que acudieron a consulta durante el año 1993 por molestias subjetivas oculares de largo tiempo de evolución. Se diagnóstico la disminución de la secreción de la secreción lagrimal mediante la prueba de Schirmer. Se realizaron 60 citologías de impresión conjuntival a 30 casos controles sanos. Posteriormente se les practicó a los pacientes la citología conjuntival biopsia de glándulas salivales accesorias del labio inferior, presentaron diversas grados de afectación en ambas pruebas y se comprobó, además, una relación directa entre las mismas, ya que siempre que el cuadro citológico conjuntival se observaba afectado severamente. También lo estabn en igual grado los hallazgos histológicos de la biopsia

La citologia de impresion conjuntival y la biopsia de la glandula salival accesoria en el sindrome de ojo seco / Conjunctival impression cytology and biopsy of the accesory salival gland in the syndrome of the dry eye

Se realizo una investigacion en 7 pacientes con el sindrome de ojo seco, cuyas edades estaban comprendidas entre los 24 y 60 anos, con un solo integrante del sexo masculino y que acudieron a consulta durante el ano 1993 por molestias subjetivas oculares de largo tiempo de evolucion. Se diagnostico la disminucion de la secrecion de la secrecion lagrimal mediante la prueba de Schirmer. Se realizaron 60 citologias de impresion conjuntival a 30 casos controles sanos. Posteriormente se les practico a los pacientes la citologia conjuntival biopsia de glandulas salivales accesorias del labio inferior, presentaron diversas grados de afectacion en ambas pruebas y se comprobo, ademas, una relacion directa entre las mismas, ya que siempre que el cuadro citologico conjuntival se observaba afectado severamente. Tambien lo estabn en igual grado los hallazgos histologicos de la biopsia

Histoplasmosis de forma seudotumoral. Presentación de un caso / Histoplasmosis with pseudotumoral form. A case report

Se presenta un caso de histoplasmosis de forma seudotumoral, detectado en el acto quirúrgico, en la parrilla izquierda de un paciente con antecedente de haber trabajado en cuevas. La impresión diagnóstica clínica fue de neoplasia maligna. Se le aplicó después de la intervención quirúrgica miconazol. El paciente estuvo asintomático al cabo de 15 días. Se revisa la literatura médica (AU)