Genotoxicity assessment of new synthesized acridine derivative--3,6-diamino-10-methyl-9,10-dihydroacridine.

A new synthesized acridine derivative, 3,6-diamino-10-methyl-9, 10-dihydroacridine (AcrH), was tested for in vitro reverse mutations with Salmonella TA strains, chromosome aberrations and sister chromatid exchanges (SCE) in human lymphocytes, and for in vivo chromosome aberrations in bone marrow of mice. Using the classic plate incorporation method, mutagenicity of AcrH in bacterial cells (TA97a, TA98, TA100 and TA102) was observed in the experiments performed with, and without, rat liver S9 metabolic activation. The reverse mutation assay showed no difference in mutagenic activity between AcrH and acriflavine (Acr(+)) in the test with TA97. The results of in vitro chromosome aberrations assay revealed potential clastogenicity. The test using macroculture of human lymphocytes induced mainly chromatid gaps. The experiments with human lymphocytes revealed SCE-inducing effect of AcrH and Acr(+). In an in vivo study, AcrH given intraperitoneally to Balb/c mice did not cause any significant increase in the percentage of cells with aberrations compared to the negative control.

Radicals as probes of ferroelectric phase transitions in EPR investigations of pyridinium tetrafluoroborate.

This paper reports CW-EPR studies of pyridinium tetrafluoroborate undergoing solid-solid phase transitions at 240 and 205 K, of which the one occurring at the higher temperature was found to be paraelectric-ferroelectric. EPR spectra of gamma-irradiated polycrystalline samples were taken at room temperature, at the liquid nitrogen temperature, and at temperatures ranging from 163 to 273 K in the cycles of cooling and heating. The radical appearing under the effect of gamma irradiation was established to be formed by attachment of a hydrogen atom to the carbon atom C(2) in the pyridine ring. The unpaired electron was found to delocalize onto the pyridine ring. The g factor was established as 2.0021(4) and the values of the hyperfine splitting constants testify to the occurrence of an alpha-type interaction, Aalpha = 0.98(5) mT, of the unpaired electron with protons at the C(3), C(5), and N(1) positions, two beta1- and beta2-type interactions with the hydrogen nuclei at the C(2) position, Abeta1 = 6.68(5) mT and Abeta2 = 4.85(5) mT, and the interaction with the nitrogen nucleus at N(1), AN = 0.86(5) mT. The complex character of the spectra obtained prompted us to employ computer analysis of the signals to enhance their resolution. Analysis of the temperature dependence of the linewidth parameter of individual components of the pyridine radical spectrum shows that EPR together with the computer resolution enhancement method can be a useful method for studying the nature of phase transitions in pyridinium tetrafluoroborate.

[Aberration of somatic karyotype in normozoospermic males from infertile couples]. / Aberracje kariotypu somatycznego u mezczyzn z normozoospermia z nieplodnych malzenstw.

During the complex investigation of couple infertility, a group of 40 normozoospermic males having low density of semen was selected. Cytogenetic analysis revealed 2 cases of somatic karyotype aberrations: 47,XYY and 46,XY,t(14;19), and 2 cases of chromosomal polymorphisms: 46,XYq+ i 46,XY,14p+. In the article the mechanism of fertility disturbances evoked by these findings is discussed in aspects of the function of spermatozoa and its genetic material content.