Whey protein concentrate and skimmed milk powder as encapsulation agents for coffee silverskin extracts processed by spray drying.

We tested the hypothesis that milk proteins, through microencapsulation, guarantee protection against bioactive substances in coffee silverskin extracts. Therefore, the aim of this study was to carry out technological, nutritional and physicochemical characterisation of a coffee silverskin extract microencapsulated using instant skim milk powder and whey protein concentrate as wall materials. The aqueous extract of coffee silverskin was spray-dried using 10% (w/v) skim milk powder and whey protein concentrate. The samples were characterised by determining the water content, water activity, particle size distribution, colour analysis and total phenolic compound content as well as antioxidant activity using 2,2-diphenyl-radical 1-picrylhydrazyl scavenging methods, nitric oxide radical inhibition and morphological analysis. The product showed water activity within a range that ensured greater stability, and the reduced degradation of the dried coffee silverskin extract with whey protein concentrate resulted in better rehydration ability. The luminosity parameter was higher and the browning index was lower for the encapsulated samples than for the pure coffee silverskin extract. The phenolic compound content (29.23 ± 8.39 and 34.00 ± 8.38 mg gallic acid equivalents/g for the coffee silverskin extract using skimmed milk powder and whey protein concentrate, respectively) and the antioxidant activity of the new product confirmed its potential as a natural source of antioxidant phenolic compounds. We conclude that the dairy matrices associated with spray drying preserved the bioactive and antioxidant activities of coffee silverskin extracts.

Molecular genotyping reveals inter-regional relatedness among antimicrobial resistant Salmonella Minnesota strains isolated from poultry farm and humans, Brazil.

Genetic profiles of Salmonella Minnesota isolates were analyzed using pulsed-field gel electrophoresis (PFGE). In total, 13 isolates obtained from the broiler industry collected in the states of Minas Gerais (11) and São Paulo (2), as well as five recovered from cases of foodborne infections in humans in the states of Minas Gerais (2), Santa Catarina (1), and Rio Grande do Sul (2), were submitted to PFGE. These 18 S. Minnesota isolates together with other 12 of poultry origin were also subjected to antimicrobial susceptibility testing. The PFGE analysis of 18 strains of S. Minnesota generated a dendrogram that grouped the isolates with 83-90% similarity into four main clusters. Among them, cluster "A" grouped the majority of isolates (13), including two of human origin that showed 90% similarity with a broiler isolate, both recovered in Minas Gerais. The S. Minnesota isolates showed resistance to tetracycline (80%), cefoxitin (80%), ceftazidime (46.7%), nalidixic acid (23.3%), ciprofloxacin (13.3%), and streptomycin (10%). No resistance to gentamicin, chloramphenicol, meropenem, nitrofurantoin, and sulfamethoxazole-trimethoprim was found. Moreover, 23.3% of the evaluated isolates presented multi-resistance profile, all from Minas Gerais. The results highlight the importance of further studies involving S. Minnesota, which is prevalent in the Brazilian broiler flocks and could provoke foodborne infection in humans.

Adjusting RT-qPCR conditions to avoid unspecific amplification in SARS-CoV-2 diagnosis.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in December 2019 and quickly spread around the world, forcing global health authorities to develop protocols for its diagnosis. Here we report dimer formation in the N2 primers-probe set (CDC 2019-nCoV Real-Time RT-PCR) used in the diagnostic routine, and propose alternatives to reduce dimerization events. Late unspecific amplifications were visualized in 56.4% of negative samples and 57.1% of no-template control, but not in positive samples or positive control. In silico analysis and gel electrophoresis confirmed the dimer formation. The RT-qPCR parameters were optimized and the late unspecific amplifications decreased to 11.5% in negative samples and no-template control. The adjustment of PCR parameters was essential to reduce the risk of false-positives results and to avoid inclusive results requiring repeat testing, which increases the costs and generates delays in results or even unnecessary requests for new samples.

Körperkoordinationstest Für Kinder (KTK) for Brazilian Children and Adolescents: Factor Analysis, Invariance and Factor Score.

The decrease in children motor competence, with a consequent reduction in the levels of physical activities and fitness, impacting health negatively, has affected children across countries. In addition to consistent intervention strategies, it is necessary to use appropriate instruments. The Körperkoordinationstest Für Kinder (KTK) is a reliable and low-cost motor coordination (MC) test used in several countries but lacking psychometric evidence in the Brazilian population. The present study investigates the factor structure of KTK in a Brazilian sample; and, compared four possibilities of calculating the factorial score of the test, precisely the sum of the scores, sum of the standard scores, weighted method, and the refined method. The participants of the study consisted of 565 volunteers (49.9% boys), from 5 to 10 (7.93 ± 1.51) years of age, with a body mass index (BMI) means of 17.04 (±2.81). The results showed that the KTK factor structure was adequate to the model for the total sample, by sex, and by age groups. However, the results did not confirm the invariance between sexes and age groups. Besides, our result showed that the sum of the raw scores of the subtests could be used as the factor score method in KTK. In the end, we conclude that the KTK is a valid test to measure the MC of Brazilian children and adolescents, with features that qualify it as a useful instrument both for research and for the practice.

Relative age effect and constituent year effect: an analysis of the international tennis federation ranking / Efeito da idade relativa e efeito do ano constituinte: uma análise do ranking da federação internacional de tênis

ABSTRACT Relative Age Effect (RAE) and Constituent Year Effect (CYE) seem to influence the process of training young tennis players. The aim of this study was to investigate the presence of these effects in the ranking of junior tennis players of the International Tennis Federation (ITF) and its influence on the score obtained by such tennis players. The results show the presence of the RAE and the CYE (p ≤ 0.05). The regression model identified that the variables "year" and "month" of birth predict the scores of athletes in the ITF ranking with an explained variance of 19%. In conclusion, the ranking composed of various age groups does not seem to be a classification strategy sensitive enough to prevent the RAE and the CYE among junior tennis players.

RESUMO O Efeito da Idade Relativa (EIR) e o Efeito do Ano Constituinte (EAC) parecem influenciar o processo de formação de jovens tenistas.O objetivo do presente estudo foi investigar a presença desses efeitos no ranking de tenistas juniores da InternationalTennisFederation (ITF) e sua influência na pontuação obtida pelos tenistas. Os resultados encontrados demonstram a presença do EIR e do EAC (p ≤ 0,05). O modelo de regressão identificou que as variáveis "ano" e "mês" de nascimento predizem a pontuação dos atletas no ranking da ITF com uma variância explicada de 19%. Em conclusão, o ranking formado por várias faixas etárias não tem se mostrado uma estratégia de classificação sensível o bastante para prevenir o EIR e o EAC entre os tenistas juniores.

The influence of technical skills on decision making of novice volleyball players / A influência da habilidade técnica na tomada de decisão de jogadores iniciantes de voleibol

Abstract The aim of this study was to verify the influence of the technical skills level on the decision making of novice volleyball players on service, reception and set actions. The sample was composed of 80 novice athletes of school volleyball teams of both sexes. Based on the results from the Volleyball Skills Test Battery, the sample was divided into three groups: low, intermediate and high technical level, by means of One-Way ANOVA. The intermediate group was excluded from further analyses. Decision making was assessed with the Game Performance Assessment Instrument (GPAI). For data analysis purposes, the independent student-t test was used, adopting significance level of p≤0.05. The results showed that players with low skill level in service and set actions also had lower performance on the decision-making test when compared to players with high technical level. On the other hand, in the reception action, no differences were found among groups. It was therefore concluded that the technical level seems to influence the decision making in service and set actions in novice volleyball players.

Resumo O estudo objetivou verificar a influência do nível da habilidade técnica na tomada de decisão de jogadores iniciantes de voleibol nas ações de saque, recepção e levantamento. A amostra foi composta por 80 atletas iniciantes de equipes escolares de voleibol de ambos os sexos. Com base nos resultados na Bateria de Testes de Habilidades de Voleibol, a amostra foi dividida em três grupos: baixo, intermediário e alto nível técnico, por meio da ANOVA One-Way. O grupo intermediário foi excluído das análises posteriores. A tomada de decisão foi avaliada por meio do Game Performance Assessment Instrument (GPAI). Utilizou-se o teste t student independente para análise dos dados, adotando um nível de significância de p≤0,05. Os resultados encontrados demonstraram que os jogadores com baixo nível de habilidade nas ações de saque e levantamento, também obtiveram um pior desempenho no teste de tomada de decisão quando comparado aos jogadores de alto nível técnico. Por outro lado, na ação de recepção, não foram encontradas diferenças significativas entre os grupos. Conclui-se que o nível técnico parece influenciar na tomada de decisão na ação de saque e levantamento dos jogadores iniciantes de voleibol.

Interferon lambda and hepatitis C virus core protein polymorphisms associated with liver cancer.

BACKGROUND: Hepatitis C virus (HCV) infection is often persistent and gradually advances from chronic hepatitis to liver cirrhosis and hepatocellular carcinoma (HCC). Worldwide, hepatocellular carcinoma is the fifth most common neoplasm. METHOD OF STUDY: the Interferon lambda (IFNL) polymorphisms genotypes (rs8099917, rs12979860 and rs12980275) and the presence of mutations in HCV core protein were analyzed in 59 patients with HCC, and also in 50 cirrhotic patients (without HCC). RESULTS: the rs12980275-AG genotype was associated with HCC on age-adjusted analysis (OR 2.42, 95% CI 1.03-5.69, P=0.043). Core substitutions R70Q and L91M were mainly found in genotype 1b isolates. Furthermore, a borderline level of statistical significance association was found among the presence of amino acid Glutamine (Q) in the position 70 and IFNL3 genotype AG (P=0.054). CONCLUSIONS: the screening of these polymorphisms and functional studies would be useful in clinical practice for identifying groups at high risk of HCC development.

Evaluation of real-time PCR assay to detect Schistosoma mansoni infections in a low endemic setting.

BACKGROUND: Schistosomiasis constitutes a major public health problem, and 200 million people are estimated to be infected with schistosomiasis worldwide. In Brazil, schistosomiasis has been reported in 19 states, showing areas of high and medium endemicity and a wide range of areas of low endemicity (ALE). Barra Mansa in Rio de Janeiro state has an estimated prevalence of 1%. ALE represent a new challenge for the helminth control because about 75% of infected individuals are asymptomatic and infections occur with a low parasite load (<100 eggs per gram of feces), causing a decrease in sensitivity of stool parasitological techniques, which are a reference for the laboratory diagnosis of this helminth. The objective of this study was to evaluate the performance of a TaqMan quantitative polymerase chain reaction (qPCR) technique in serum and feces DNA samples using the techniques of Kato-Katz (KK), Hoffman, Pons and Janer (HH) as references, during an epidemiological survey using fecal samples and sera from randomized residents from an ALE. METHODS: A cross-sectional study conducted from April to December 2011 using a probabilistic sampling that collected 572 fecal and serum samples. The laboratory diagnostic techniques used were: KK, HH and qPCR (feces and serum). RESULTS: We obtained the following results using the different diagnostic techniques: KK and HH, 0.9% (n =5); qPCR-feces, 9.6% (n =55); and qPCR-serum, 1.4% (n =8). The qPCR-feces presented the highest positivity, whereas the techniques of HH and KK were the least sensitive to detect infections (0.8%). Compared to HH and KK, qPCR-feces showed a statistically significant difference in positivity (p <0.05), although with poor agreement. CONCLUSION: The positivity rate presented by the qPCR approach was far higher than that obtained by parasitological techniques. The lack of adequate surveillance in ALE of schistosomiasis indicates a high possibility of these areas being actually of medium and high endemicity. This study presents a control perspective, pointing to the possibility of using combined laboratory tools in the diagnosis of schistosomiasis in ALE.