1.
Angew Chem Int Ed Engl
; 51(44): 10997-1001, 2012 Oct 29.
Artigo
em Inglês
| MEDLINE
| ID: mdl-23012075
RESUMO
Mannosides in the southern hemisphere: Conformational analysis of enzymatic mannoside hydrolysis informs strategies for enzyme inhibition and inspires solutions to mannoside synthesis. Atomic resolution structures along the reaction coordinate of an inverting α-mannosidase show how the enzyme distorts the substrate and transition state. QM/MM calculations reveal how the free energy landscape of isolated α-D-mannose is molded on enzyme to only allow one conformationally accessible reaction coordinate.