A program to enhance recruitment and retention of disadvantaged and ethnically diverse baccalaureate nursing students.

PURPOSE: To describe and evaluate the use of a "Success in Learning: Individualized Pathways Program (SLIPP)" to retain and graduate disadvantaged and ethnically diverse nursing students. DESIGN: A summative evaluative design was used with a population of 77 disadvantaged and ethnically diverse students who were accepted into a pre-entrance preparation quarter. The program based on an academic success model, included six pre-entrance classes, academic, social, and financial support, and seven faculty development workshops. Program outcomes were studied using student records, survey results, and interviews. RESULTS: Following the pre-entrance quarter, all 77 students were accepted into the baccalaureate nursing program, 90.9% graduated with either a Bachelor in Science (75.3%) or Associate in Science (15.6%), and 98.6% of the graduates passed the state board registered nursing examination. DISCUSSION: Outcomes are discussed in light of similar programs. CONCLUSIONS: Underprepared disadvantaged and ethnically diverse students can successfully become registered nurses. IMPLICATIONS: Educators and recruiters for nursing practice should accept/hire culturally diverse students/nurses to expand the ethnic diversity of the nursing workforce to meet the needs of culturally diverse clients. Research is needed to determine the classes/components and length of the pre-entrance preparation program to successfully enhance success.

Age and apolipoprotein E ε4 effects on neural correlates of odor memory.

Alzheimer's disease (AD) affects 5.4 million Americans. Evidence suggests that individuals who are positive for the apolipoprotein E (ApoE) ε4 allele are at higher risk for developing the disease. Studies have also shown that the ε4 allele is linked to olfactory decline. Olfactory functioning may be investigated using olfactory event-related potentials (OERPs). The high temporal resolution of OERPs enables an understanding of the neural correlates of olfactory processing and functioning. This study investigated the effects of age, ApoE ε4 status, response type, and electrode site on OERP latency and amplitude during encoding and retrieval in an odor recognition memory task. The 60 participants were equally divided into 3 age groups matched on ε4 status: younger, middle, and older. Odors were presented using a computer-controlled olfactometer. Participants were notified during encoding that this was a task of odor memory. Results indicated differences in OERP activity as a function of age, ApoE ε4 status, response type, and electrode site. These findings highlight the potential of OERPs to distinguish ε4- and ε4+ individuals and to contribute to an earlier diagnosis of AD.

Pin1 regulates the dynamics of c-Myc DNA binding to facilitate target gene regulation and oncogenesis.

The Myc oncoprotein is considered a master regulator of gene transcription by virtue of its ability to modulate the expression of a large percentage of all genes. However, mechanisms that direct Myc's recruitment to DNA and target gene selection to elicit specific cellular functions have not been well elucidated. Here, we report that the Pin1 prolyl isomerase enhances recruitment of serine 62-phosphorylated Myc and its coactivators to select promoters during gene activation, followed by promoting Myc's release associated with its degradation. This facilitates Myc's activation of genes involved in cell growth and metabolism, resulting in enhanced proproliferative activity, even while controlling Myc levels. In cancer cells with impaired Myc degradation, Pin1 still enhances Myc DNA binding, although it no longer facilitates Myc degradation. Thus, we find that Pin1 and Myc are cooverexpressed in cancer, and this drives a gene expression pattern that we show is enriched in poor-outcome breast cancer subtypes. This study provides new insight into mechanisms regulating Myc DNA binding and oncogenic activity, it reveals a novel role for Pin1 in the regulation of transcription factors, and it elucidates a mechanism that can contribute to oncogenic cooperation between Pin1 and Myc.

Individuals at risk for Alzheimer's disease show differential patterns of ERP brain activation during odor identification.

BACKGROUND: Studies suggest that older adults at risk of developing Alzheimer's disease may show olfactory processing deficits before other signs of dementia appear. METHODS: We studied 60 healthy non-demented individuals, half of whom were positive for the genetic risk factor the Apolipoprotein E ε4 allele, in three different age groups. Event-related potentials to visual and olfactory identification tasks were recorded and analyzed for latency and amplitude differences, and plotted via topographical maps. RESULTS: Varying patterns of brain activation were observed over the post-stimulus epoch for ε4- versus ε4+ individuals on topographical maps. Individuals with the ε4 allele demonstrated different ERP peak latencies during identification of olfactory but not visual stimuli. High correct ApoE classification rates were obtained utilizing the olfactory ERP. CONCLUSIONS: Olfactory ERPs demonstrate functional decline in individuals at risk for Alzheimer's disease at much earlier ages than previously observed, suggesting the potential for pre-clinical detection of AD at very early stages.

Abnormal event-related potentials in young and middle-aged adults with the ApoE ε4 allele.

The largest genetic susceptibility factor for Alzheimer's disease is the Apolipoprotein E (ApoE) ε4 allele. Cognitive decline and olfactory impairment are greater in those positive for the ε4 allele. This study sought to determine if the olfactory event-related potential (OERP), compared to the visual ERP, would be sensitive to these subtle declines. Participants included 40 individuals from two age groups, half of each group were ε4 allele positive and half were ε4 negative. Visual ERPs did not demonstrate significant differences between ApoE groups. OERPs demonstrated robust age by ApoE interactions. P3 latencies were significantly longer in ε4 young and middle age participants. These findings suggest that very early olfactory and cognitive changes related to ApoE status are detectible via the OERP.

Differential effects of active attention and age on event-related potentials to visual and olfactory stimuli.

Normal aging impairs olfactory functioning both centrally and peripherally. The P3 peak of the event-related potential (ERP), evoked by active response to a target stimulus, is considered a reflection of central cognitive processing. It can also be evoked in a passive task to both auditory and visual stimuli. Our goal was to investigate whether age influences amplitude and latency of the ERP differentially in active and passive tasks to olfactory stimuli. Olfactory and visual event-related potentials were elicited with a single stimulus paradigm in separate active and passive task response conditions. Participants included 30 healthy individuals from three age groups, young, middle age, and older adults. Results indicated that P3 ERP latency increased with age in both sensory modalities. P3 latencies for active versus passive tasks were similar across age groups for visual ERPs, but in the olfactory modality, older adults demonstrated significantly longer latencies in the passive task compared to the active task. Future directions should include research on specific clinical populations utilizing active versus passive task conditions.

Olfaction in aging and Alzheimer's disease: event-related potentials to a cross-modal odor-recognition memory task discriminate ApoE epsilon4+ and ApoE epsilon 4- individuals.

Alzheimer's disease (AD) is a devastating neurodegenerative condition that affects more than 5 million Americans. Currently, a definitive and unequivocal diagnosis of AD can only be confirmed histopathogically via postmortem autopsy, demonstrating the need for objective measures of cognitive functioning for those at risk for AD. The single most important genetic risk factor of AD is the apolipoprotein E (ApoE) epsilon4 allele. The present study investigated olfactory and cognitive processing deficits in ApoE epsilon4(+) individuals using a cross-modal recognition memory task and an objective electrophysiological measure, the event-related potential (ERP). Ten epsilon4(+) individuals (5 M, 5 F, mean [M]= 75.1 years) and 10 age- and gender-matched epsilon4(-) individuals (5 M, 5 F, M = 71 years) sequentially encoded a set of 16 olfactory stimuli and were subsequently shown names of odors previously presented (targets) or not (foils). EEG activity was recorded from 19 electrodes as participants distinguished targets from foils using a two-button mouse. P3 latencies were significantly longer in epsilon4(+) individuals, and intraclass correlations demonstrated differential activity between the two groups. These findings are consistent with a compensatory hypothesis, which posits that nondemented epsilon4(+) individuals will expend greater effort in cognitive processing or engage in alternative strategies and therefore require greater activation of neural tissue or recruitment of different neural populations. The findings also suggest that cross-modal ERP studies of recognition memory discriminate early neurocognitive changes in ApoE epsilon4(+) and ApoE epsilon4(-) individuals and may contribute to identifying the phenotype of persons who will develop Alzheimer's disease.

The Axin1 scaffold protein promotes formation of a degradation complex for c-Myc.

Expression of the c-Myc proto-oncoprotein is tightly regulated in normal cells. Phosphorylation at two conserved residues, threonine58 (T58) and serine62 (S62), regulates c-Myc protein stability. In cancer cells, c-Myc can become aberrantly stabilized associated with altered T58 and S62 phosphorylation. A complex signalling cascade involving GSK3beta kinase, the Pin1 prolyl isomerase, and the PP2A-B56alpha phosphatase controls phosphorylation at these sites. We report here a novel role for the tumour suppressor scaffold protein Axin1 in facilitating the formation of a degradation complex for c-Myc containing GSK3beta, Pin1, and PP2A-B56alpha. Although knockdown of Axin1 decreases the association of c-Myc with these proteins, reduces T58 and enhances S62 phosphorylation, and increases c-Myc stability, acute expression of Axin1 reduces c-Myc levels and suppresses c-Myc transcriptional activity. Moreover, the regulation of c-Myc by Axin1 is impaired in several tested cancer cell lines with known stabilization of c-Myc or loss of Axin1. This study provides critical insight into the regulation of c-Myc expression, how this can be disrupted in three cancer types, and adds to our knowledge of the tumour suppressor activity of Axin1.

The relationship between internet activities and depressive symptoms in a sample of college freshmen.

An Internet survey of college freshmen at a mid-Atlantic mid-sized university was conducted during the spring of 2002 to determine the impact of Internet activities on social support and well-being. Results obtained from the survey allow examination of the impact of amount of time performing different types of Internet activities on depressive symptoms, as measured by the Iowa version of the Center for Epidemiologic Studies Depression Scale (CES-D) via a semi-elasticity ordinary least squares regression model. Results indicate that increased e-mail and chat room/instant messaging (IM) hours are associated with decreased depressive symptoms, while increased Internet hours for shopping, playing games, or research is associated with increased depressive symptoms. The implications of these results for institutions of higher education, and Internet and health researchers are discussed.

Olfactory event-related potentials in Alzheimer's disease.

Areas of the brain affected in the early stages of Alzheimer's disease are also areas heavily involved in the processing of olfactory information. Olfactory event-related potentials (OERPs) and auditory ERPs were recorded from the Fz, Cz, and Pz electrode sites in 12 Alzheimer's disease (AD) patients and 12 age and gender matched normal controls (NC) in a single-stimulus paradigm with a 45 s inter-trial interval, using amyl acetate as the olfactory stimulus, and in a separate session a 500 Hz tone as the auditory stimulus. Odor identification (ID) was also used to assess ability to identify odors. The results indicate that (1) OERP P2 and P3 latencies were significantly longer in AD patients than normal controls; (2) olfactory ERP latency measures correlated significantly with dementia status as measured by the Dementia Rating Scale (DRS), indicating that as participants performed more poorly on the DRS, reflecting increased dementia, OERP latencies increased; (3) olfactory ERP latency measures better differentiated AD patients from normal controls than auditory ERP latency measures; (4) olfactory ERP measures alone correctly classified up to 92% of participants; (5) odor ID measures, namely the UPSIT and San Diego-Odor-ID tests also classified participants at a high rate. Combining scores for odor identification with olfactory P3 latency measures resulted in a correct classification rate of 100%. The results strongly support the use of olfactory measures in the assessment of AD.