RESUMO
Pyridinium cesium cobalt nitrate, (PyH)CsCo2(NO3)6, obtained from a nitric acid solution crystallizes in the orthorhombic space group Pnma with unit cell parameters a = 8.6905(14) Å, b = 11.9599(18) Å, c = 18.386(3) Å, V = 1911.0(5) Å3, and Z = 4. It consists of [Co(NO3)3]- layers, in which each Co2+ ion is connected with four monodentate bridging NO3-groups and one bidentate terminal NO3-group, forming a corrugated rectangular net. Magnetization and specific heat measurements show that (PyH)CsCo2(NO3)6 undergoes a long-range canted antiferromagnetic ordering in two steps at TC1 = 5.0 K and TC2 = 2.6 K. The temperature dependence of the magnetic susceptibility and the field dependence of the magnetization measured for (PyH)CsCo2(NO3)6 show that it is an Ising antiferromagnet. In support of these observations, our DFT + U + SOC calculations show that the Co2+ ions of (PyH)CsCo2(NO3)6 have an easy-axis magnetic anisotropy with preferred spin orientation along the b-axis. To a first approximation, the spin lattice of (PyH)CsCo2(NO3)6 is a weakly alternating Ising antiferromagnetic chain (J1/J2 â¼ 0.85), and these chains interact weakly (J3/J2 â¼ 0.07) to form a rectangular Ising antiferromagnetic lattice. In agreement with the prediction for a rectangular Ising antiferromagnet by Onsager, (PyH)CsCo2(NO3)6 undergoes a long-range antiferromagnetic ordering.
RESUMO
Homozygous siblings with different treatment histories represent an excellent model to study both the phenotypic manifestation of mutations and the efficacy of therapy. We compared phenylketonuria (PKU) manifestations in two different gender siblings who were homozygous carriers of a rare phenylalanine hydroxylase (PAH) mutation, p.R155H, subjected to different treatments. PKU caused by mild mutations may be easily underdiagnosed if the diagnosis is based solely on the phenylalanine (Phe) blood concentration. One of the described patients is an example of this diagnostic error. For reducing diagnostic errors, we suggest the use of more elaborate methods in screening practice, in particular mass spectrometric analysis of blood metabolites, the efficiency of which is demonstrated in the present study.
RESUMO
In this work, we compare the resolution of V2-V3 and V3-V4 16S rRNA regions for the purposes of estimating microbial community diversity using paired-end Illumina MiSeq reads, and show that the fragment, including V2 and V3 regions, has higher resolution for lower-rank taxa (genera and species). It allows for a more precise distance-based clustering of reads into species-level OTUs. Statistically convergent estimates of the diversity of major species (defined as those that together are covered by 95% of reads) can be achieved at the sample sizes of 10000 to 15000 reads. The relative error of the Shannon index estimate for this condition is lower than 4%.
Assuntos
Código de Barras de DNA Taxonômico/métodos , Metagenômica/métodos , Microbiota/genética , RNA Ribossômico 16S/genética , Microbiologia da Água , Bactérias/genética , Biologia Computacional/métodos , Sequenciamento de Nucleotídeos em Larga Escala , Lagos , Federação Russa , Análise de Sequência de DNARESUMO
Ionic liquids that are stable toward oxidation and nitration and are based on the 1-n-butyl-3-methylimidazolium cation (BMIm+) can be used as solvents and reaction media for copper dissolution in liquid dinitrogen tetraoxide N2O4. The ionic liquid not only favors the dissociation of N2O4 into NO+ and NO3-, but also takes part in the formation of different crystalline products. Thus, NO[BF4], NO[Cu(NO3)3] and (BMIm)2[Cu2(CF3COO)6] were prepared using (BMIm)A, A- = [BF4]-, (CF3SO2)2N-, CF3COO-, respectively. The formation of a certain product is determined by the nature of the anion A- and the relative solubility of the reaction products in the ionic liquid. Crystals of NO[BF4] were also prepared directly from a mixture of N2O4 and BMImBF4. According to XRD single-crystal structure analysis, the structure of NO[BF4] consists of tetrahedral [BF4]- anions and nitrosonium NO+ cations; the formation of these ions prove the heterolytic dissociation of N2O4 dissolved in the ionic liquid. The crystal structure of the earlier unknown binuclear copper trifluoroacetate (BMIm)2[Cu2(CF3COO)6] were determined by X-ray diffraction. The peculiarity of this dimer compared to the majority of known dimeric copper(ii) carboxylates is the unusually long CuCu distance (3.15 Å), with Cu(ii) ions demonstrating an atypical coordination of a distorted trigonal bipyramid formed by five O atoms of five trifluoroacetate groups.
RESUMO
Bacterial mats formed by a colorless sulfur bacterium Thioploca sp. in the area of the Posolski Bank cold methane seep (southern Baikal) were -studied using electron microscopy and phylogenetic analysis. Morphologically the bacteria were identified as Thioploca ingrica.- Confocal microscopy of DAPI-stained samples revealed numerous rod-shaped, filamentous, and spiral microorganisms in the sheaths, as well as in- side and between the trichomes. Transmission electron microscopy revealed nonvacuolated bacteria and small cells-without cell envelopes within the sheath. Bacteria with pronounced intracytoplasmic membranes characteristic; of type I methanotrophs were observed at the outer side of the sheath. Based on analysis of the 16S rRNA gene sequences, the following phyla were idenified in the sheath community: Bacteroidetes, Nitro- spira, Chloroflexi, Planctomycetes, Verrucomicrobia,'y-, and 6-Proteobacteria, Euryarchaeota, Crenarchaeota, and Thaumarchaeota, as well as anammox bacteria. A hypothetical scheme of matter flows in the Lake Baikal bacterial mats was proposed based on the data on metabolism of the cultured homologues.
Assuntos
Água Subterrânea/microbiologia , Lagos/microbiologia , Consórcios Microbianos/fisiologia , RNA Ribossômico 16S/genética , Thiotrichaceae/genética , Bacteroidetes/classificação , Bacteroidetes/genética , Bacteroidetes/isolamento & purificação , Chloroflexi/classificação , Chloroflexi/genética , Chloroflexi/isolamento & purificação , Crenarchaeota/classificação , Crenarchaeota/genética , Crenarchaeota/isolamento & purificação , Ecossistema , Euryarchaeota/classificação , Euryarchaeota/genética , Euryarchaeota/isolamento & purificação , Metano/química , Metano/metabolismo , Filogenia , Planctomycetales/classificação , Planctomycetales/genética , Planctomycetales/isolamento & purificação , Proteobactérias/classificação , Proteobactérias/genética , Proteobactérias/isolamento & purificação , Sibéria , Thiotrichaceae/classificação , Thiotrichaceae/isolamento & purificação , Thiotrichaceae/ultraestrutura , Verrucomicrobia/classificação , Verrucomicrobia/genética , Verrucomicrobia/isolamento & purificaçãoRESUMO
Production of gaseous hydrocarbons by the microbial community of the Posolsky Bank methane seep bottom sediments (Southern Baikal) was studied at 4°C. Formation of both methane and a heavier gas- eous hydrocarbon, ethane, was detected in enrichment cultures. The highest methane concentrations (6.15 and 4.51 mmol L(-1)) were revealed in enrichments from the sediments from 55-cm depth incubated with-so- dium acetate and H2/CO2 gas mixture, respectively. A decrease in activity of aceticlastic methanogensand a decrease in methane concentration produced by hydrogenotrophic archaea occurred with depth. The highest concentration of ethane was revealed in enrichments from the microbial community of the layer close to gas hydrates (75 cm) incubated with CO2 as a substrate. According to analysis of the 16S rRNA gene fragments from the clone library, these enrichments were found to contain members of the phylum Crenarchaeota form- ing a separate cluster with members of the class Thermoprotei. The phylum Euryarchaeota was represented by nucleotide sequences of the organisms homologous to members of the orders Methanococcales, Methanosa- rcinales, and Thermoplasmatales.
Assuntos
Archaea/metabolismo , Sedimentos Geológicos/microbiologia , Hidrocarbonetos/metabolismo , Filogenia , Archaea/genética , Dióxido de Carbono/metabolismo , Crenarchaeota/genética , Crenarchaeota/isolamento & purificação , Crenarchaeota/metabolismo , Etano/metabolismo , Euryarchaeota/genética , Euryarchaeota/isolamento & purificação , Euryarchaeota/metabolismo , Biblioteca Gênica , Lagos , Metano/metabolismo , Consórcios Microbianos , Dados de Sequência Molecular , RNA Ribossômico 16S , SibériaRESUMO
At the site of natural ingress of oil microbial diversity in the Central Baikal bottom sediments differing in the chemical composition of pore waters was studied by molecular biological techniques. The sediments saturated with oil and methane were found to contain members of 10 bacterial and 2 archaeal phyla. The oxidized sediment layer contained methanotrophic bacteria belonging to the Alphaproteobacteria, which had a specific structure of the pmoA gene and clustered together with uncultured methanotrophs from cold ecosystems. The upper sediment layer contained also oil-oxidizing bacteria and the alkB genes most colsely related to those of Rhodococcus. The microbial community of reduced sediments exhibited lower diversity and was represented mostly by the organisms involved in hydrocarbon biodegradation.
Assuntos
Archaea/metabolismo , Bactérias/isolamento & purificação , Sedimentos Geológicos/microbiologia , Lagos/microbiologia , Archaea/genética , Archaea/isolamento & purificação , Bactérias/genética , Bactérias/metabolismo , Biodegradação Ambiental , Ecossistema , Proteínas de Escherichia coli/genética , Água Doce/microbiologia , Genes Bacterianos , Lagos/química , Metano/metabolismo , Oxigenases de Função Mista/genética , Dados de Sequência Molecular , Petróleo , Filogenia , Piperazinas , RNA Ribossômico 16S , SibériaRESUMO
Adult mice of the BALB/cLac, PT, CBA/Lac, DD/He, A/He, SWR, NZB, GR, DBA/2J, CC57Br, C57 B1/6J, A/Sn, and YT inbred strains were tested for the count, motility, and morphology of sperms from the caudal region of the epididymis. The protein-coding regions of the cytochrome P450 aromatase (CYP19a1), estrogen receptor 2 (ESR2), steroidogenic factor 1 (Nr5a1), and sex-determining (Sry) gene were sequenced. A substantial genetic heterogeneity for the genes was observed, as well as a phenotypic variation in spermatogenetic parameters, but the variation was rather discordant. The specifics of the interstrain variation in spermatogenetic parameters indicated that a physiological compensatory mechanism increases certain spermatogenetic parameters when other ones are low to maintain male fertility at a level sufficient for successful reproduction. For instance, a high sperm production compensated for a low sperm motility in DD/He males. In the issue of the protein-coding regions sequencing of the analyzed genes, 16 various mutations were observed. The decreases in proportion of motile sperms and in their velocity were attributed to mutations (I63T and W133L) of the Sry gene in the DD/He strain.
Assuntos
Mutação , Polimorfismo Genético , Espermatogênese/genética , Espermatozoides , Animais , Citocromo P-450 CYP1A1/genética , Receptor beta de Estrogênio/genética , Masculino , Camundongos , Camundongos Endogâmicos/genética , Camundongos Endogâmicos/fisiologia , Fenótipo , Fatores de Transcrição SOXB1/genética , Análise de Sequência de DNA , Espermatozoides/citologia , Espermatozoides/fisiologia , Fator Esteroidogênico 1/genéticaRESUMO
Phenylketonuria (PKU) associated mutations in phenylalanine hydroxylase (PAH) gene were identified by direct DNA sequencing in 46 PKU patients and members of their families from Kemerovskaya Region and Saha Republic. Mutations found included both widespread known mutations (R158Q, R252W, R261Q, P281L, IVS10-11G>A, R408W, IVS12+1G>A) and several rare mutations (IVS2+5G>A, R155H, Y168H, W187R, E221_D222>Efs, A342T, Y386C, IVS11+1G>C). We observed the increase in diversity of PKU-associated alleles in the populations studied, probably due to their complex mixed ethnic structure.
Assuntos
Povo Asiático , Mutação , Fenilalanina Hidroxilase/genética , Fenilcetonúrias/genética , População Branca , Alelos , Análise Mutacional de DNA , Éxons , Humanos , Íntrons , Fenilcetonúrias/etnologia , Reação em Cadeia da Polimerase , Polimorfismo Genético , Federação Russa/epidemiologiaRESUMO
We have studied the electronic structure of the nonmagnetic LiFeAs (T(c)â¼18 K) superconductor using angle-resolved photoemission spectroscopy. We find a notable absence of the Fermi surface nesting, strong renormalization of the conduction bands by a factor of 3, high density of states at the Fermi level caused by a van Hove singularity, and no evidence for either a static or a fluctuating order except superconductivity with in-plane isotropic energy gaps. Our observations suggest that these electronic properties capture the majority of ingredients necessary for the superconductivity in iron pnictides.
RESUMO
We report superconducting (SC) properties of stoichiometric LiFeAs (T(c)=17 K) studied by small-angle neutron scattering (SANS) and angle-resolved photoemission (ARPES). Although the vortex lattice exhibits no long-range order, well-defined SANS rocking curves indicate better ordering than in chemically doped 122 compounds. The London penetration depth lambda(ab)(0)=210+/-20 nm, determined from the magnetic field dependence of the form factor, is compared to that calculated from the ARPES band structure with no adjustable parameters. The temperature dependence of lambda(ab) is best described by a single isotropic SC gap Delta(0)=3.0+/-0.2 meV, which agrees with the ARPES value of Delta(0)(ARPES)=3.1+/-0.3 meV and corresponds to the ratio 2Delta/k(B)T(c)=4.1+/-0.3, approaching the weak-coupling limit predicted by the BCS theory. This classifies LiFeAs as a weakly coupled single-gap superconductor.
RESUMO
A plasma model of relaxation of a medium in heavy-ion tracks in condensed matter is proposed. The model is based on three assumptions: the Maxwell distribution of plasma electrons, localization of plasma inside the track nanochannel, and constant values of the plasma electron density and temperature during the x-ray irradiation. The model of multiple ionization of target atoms by a fast projectile ion is used to determine the initial conditions. An analysis of the results of the calculations performed makes it possible to define when the atomic relaxation model is a very rough approximation and the plasma relaxation model must be used. It is demonstrated that the plasma relaxation model adequately describes the x-ray spectra observed upon interaction of a fast ion with condensed target. The comparison with the experimental data justifies the reliability of the plasma relaxation model. Preassumptions of plasma relaxation model are validated by the molecular-dynamics simulation. An x-ray spectral method based on the plasma relaxation model is proposed for diagnostics of the plasma in fast ion tracks. The results obtained can be useful in examining the initial stage of defect formation in solids under irradiation with single fast heavy ions.
RESUMO
Mutation Y168H of the human phenylalanine hydroxylase (PAH) gene determining phenylketonuria was described only twice: in a patient from Catalonia (Spain) and by us in a patient from Western Siberia (Russia). The association of Y168H in these families with allelic variants of STR and VNTR repeats and a number of neutral point polymorphisms of the PHA gene (IVS3nt-22C > T, Q232Q, V245V, L385L) was studied in this work. The Y186H mutation in these families was found to be associated with different haplotypes. Strong linkage of the selected markers and the mutation region excludes recombination as a possible cause of association of Y168H with different haplotypes. It was concluded that Y168H occurred independently in different populations.
Assuntos
Ligação Genética , Mutação de Sentido Incorreto , Fenilalanina Hidroxilase/genética , Fenilcetonúrias/genética , Polimorfismo Genético , Haplótipos , Humanos , Sibéria , EspanhaRESUMO
We discuss scanning near-field optical microscope based on original double resonant montage of a fibre probe onto the tuning fork and proprietary electronics capable of fast and precise measurements of the resonant frequency and the quality factor of sensor dithering. Special emphasis is given on the pulsed excitation/gated detection of optical signal. This option as well as the possibility of fast scanning facilitates a lot the problem of single fluorescence centres detection. To illustrate the performance of this microscope, we present first true single-molecule fluorescence resonance energy transfer scanning near-field optical microscope images of single CdSe nanocrystals on glass slide surface and observation of an optical 'pseudoresolution' of densely packed 100-nm-diameter transfluorescent spheres in noisy conditions.
RESUMO
Arthropod-borne bacterial pathogen Bartonella DNA was detected in human blood cells after tick bites in summer 2003 and 2004 in Novosibirsk region by nested PCR with primers specific to groEL gene of HSP60 protein. Comparative assay of 190 p.b. of long PCR fragment revealed that the nucleotide sequences might belong to Bartonella henselae and Bartonella quintana.
Assuntos
Infecções por Bartonella/microbiologia , Bartonella/genética , DNA Bacteriano/análise , Reação em Cadeia da Polimerase/métodos , Animais , Bartonella/isolamento & purificação , Infecções por Bartonella/etiologia , Bartonella henselae/genética , Bartonella henselae/patogenicidade , Bartonella quintana/genética , Bartonella quintana/patogenicidade , Mordeduras e Picadas , Sangue/microbiologia , Humanos , Sibéria , CarrapatosRESUMO
A new approach to the identification of point mutations by allele-specific PCR was proposed. The mutation R408W of the human phenylalanine hydroxylase gene was used as a model. A high specificity of the approach was achieved by the use of primers partially complementary to the genomic DNA. Polyethylene glycol covalently attached to one of the allele-specific primers provides for the differential identification of the PCR products due to a change in electrophoretic mobility.
Assuntos
Mutação Puntual , Polimorfismo de Nucleotídeo Único , Alelos , Primers do DNA , Eletroforese em Gel de Ágar , Humanos , Fenilalanina Hidroxilase/genética , Fenilcetonúrias/genética , Reação em Cadeia da PolimeraseRESUMO
Mutations were studied in phenylalanine hydroxylase gene of phenylketonuria patients from Kemerovo oblast and Altaiskii krai (15 and 2 families, respectively). The following mutations were identified in exons of this gene: R408W, R261Q, R243Q, Y414C, Y386C, P281L, Y168H, R68S (lead to amino acid substitutions), R243X (leads to stop codon formation), and three splice site mutations (IVS12nt 1g-->a, IVS2nt-13t-->g, IVS7nt 1g-->a).