RESUMO
Synovial membrane is traditionally considered as a part of the joint capsule. It, however, differs from fibrous part of the capsule in development, structure, function, vascularisation, innervation and involvement in pathological processes. Moreover, in some areas, it even does not contact with the fibrous capsule. Thus, it appears that the synovial membrane should be considered as an independent organ and not as the lining of the joint capsule.
RESUMO
Chondrocytes differentiate from mesenchymal progenitors and produce templates(anlagen) for the developing bones. Chondrocyte differentiation is controlled by Sox transcription factors. Templates for the neighbour bones are subsequently separated by conversion of differentiated chondrocytes into non-chondrogenic cells and emergence of interzone in which joints cavitation occurs. A central role in initiating synovial joint formation plays Wnt-14/beta-catenin signalling pathway.Moreover, bone morphogenetic proteins and growth and differentiation factors are expressed at the site of joint formation. Joint cavitation is associated with increased hyaluronic acid synthesis. Hyaluronic acid facilitates tissue separation and creation of a functional joint cavity. According to the traditional view articular cartilage represents part of cartilage anlage that is not replaced by bone through endochondral ossification. Recent studies indicate, however, that peri-joint mesenchymal cells take part in interzone formation and that these interzone cells subsequently differentiate into articular chondrocytes and synovial cells. Thus,anlage chondrocytes have a transient character and disappear after cessation of growth plate function while articular chondrocytes have stable and permanent phenotype and function throughout life.
Assuntos
Cartilagem Articular/embriologia , Condrócitos/fisiologia , Articulações/embriologia , Membrana Sinovial/embriologia , Animais , Cartilagem Articular/citologia , Humanos , Articulações/citologia , Membrana Sinovial/citologiaRESUMO
Cartilage produced in 2-week-old intramuscular transplants of syngeneic chondrocytes in rats did not display any signs of rejection. Cartilage produced by similar transplants in animals presensitized with intramuscular transplants of allogeneic chondrocytes was surrounded by infiltrations composed mainly of lymphocytes and was partially resorbed. Spleen mononuclear cells (SMC) from recipients of syngeneic transplants alone were not stimulated in mixed splenocyte-chondrocyte cultures by syngeneic or allogeneic chondrocytes. SMC from recipients of allogeneic and subsequent syngeneic transplants were strongly stimulated by both syngeneic and allogeneic chondrocytes, although stimulation by the latter was significantly more pronounced. Sera from naive rats usually contained cytotoxic antichondrocyte antibodies but their level varied considerably in various individuals. In rats chosen as transplant recipients on the basis of low antichondrocyte cytotoxicity of their sera, this toxicity was markedly raised after sensitization with allo- and syngeneic chondrocytes. Absorption with thymocytes or fibroblasts decreased but did not abrogate cytotoxicity. These observations support previous reports suggesting expression of tissue-specific antigen(s) by chondrocytes.
Assuntos
Cartilagem/citologia , Condrócitos/imunologia , Condrócitos/transplante , Rejeição de Enxerto/imunologia , Rejeição de Enxerto/patologia , Animais , Anticorpos/análise , Cartilagem/imunologia , Células Cultivadas , Epitopos , Fibroblastos/citologia , Ratos , Ratos Endogâmicos Lew , Ratos Wistar , Baço/citologia , Timo/citologiaRESUMO
Abstract: The rate of fatty acid uptake, oxidation, and deposition in skeletal muscles in relation to total and unbound to albumin fatty acids concentration in the medium were investigated in the incubated rat soleus muscle. An immunohistochemical technique was applied to demonstrate whether the albumin-bound fatty acid complex from the medium penetrates well within all areas of the muscle strips. It was found that the percentage of incorporation of palmitic acid into intramuscular lipids was fairly constant, independently of the fatty acid concentration in the medium, and amounted to 63-72% for triacylglycerols, 7-12% for diacylglycerols-monoacylglycerols, and 19-26% for phospholipids. Both palmitic acid incorporation into the muscle triacylglycerol stores and its oxidation to CO2 closely correlated with an increase in both total and unbound to albumin fatty acid concentrations in the incubation medium. Under conditions of increased total but constant unbound to albumin palmitic acid concentrations, the incorporation of palmitic acid into triacylglycerols and its oxidation to CO2 were also increased, but to a lower extent. This supports the hypothesis that the cellular fatty acid metabolism depends not only on the availability of fatty acids unbound to albumin, but also on the availability of fatty acids complexed to albumin.
Assuntos
Músculo Esquelético/metabolismo , Ácido Palmítico/metabolismo , Animais , Imuno-Histoquímica , Técnicas In Vitro , Masculino , Ratos , Ratos WistarRESUMO
The purpose of this article is to present the current state of knowledge regarding the structure and functions of articular cartilage. Articular cartilage is constructed with hyaline cartilage tissue. It is composed of chondrocytes located in lacunae and the extracellular matrix. The chondrial matrix contains water, collagen, proteglycans, non-collagenous matrix proteins, and lipids. Articular cartilage is devided into four zones - superficial, intermediate, deep, and calcified - on the basic of morphology, the orientation of collagen fiber, and the proteoglycan content. The dominant collagen of this tissue is Type II collagen, which, together with smaller quantities of other collagens (i.e. Types IX and XII), forms a network of fibers, with large, aggregating proteoglycans and smaller, non-aggregating proteoglycans. Proteoglycans are proteins that contain covalently attached glycosaminoglycans (GAGs), with water between them. The large aggregating proteoglycans, called "aggrecans", form aggregates that bind hyaluronic acid, and together with collagen they are responsible for the mechanical properties of cartilage. The smallnonaggregating proteoglycans, decorin and fibromodulin, limit the formation of collagen fibres. Other proteins in the cartilage matrix - chondrocalcin and the N-propetide of Type II collagen - participate in fiber formation. Yet other proteins - chondronectin, fibronectin, vitronectin and thrombospondin - take part in the interaction between the chondrocytes and the matrix. Cartilage oligomeric matrix protein (COMP) prevents the vascularization of the cartilage and, perhaps, is responsible for the repair process. The proteins known as Cart-1 and CEP-68 participate in chondrogenesis, while tenascin and Mgp are considered to be cartilage calcification inhibitors. Apart from the structural elements, chondrocytes produce substances that fulfill purely physiological functions: enzymes and cytokines. The enzymes - which include metalloproteinases, adamalysins, serine and cysteine proteases and their inhibitors - participate in cartilage matrix reconstruction. The cytokines - IL-1, TNF-alfa, IL-6, IL-8, and LIF - stimulate the chondrocytes to produce an increased amount of enzymes, while IL-4 inhibits this process. Human articular chondrocytes exibit the constitutive expression of class I molecules of the major histocompatibility complex (MHC), molecules regulating the activation of the complement, and after activation (e.g. under the influence of IFN-alfa, IL-1, TNF-a or in the course of arthritis), also MHC class II and ICAM-1 intracellular adhesion molecules. Numerous studies have shown that chondrocytes also have tissue-specific antigens, which induce the production of antibodies in patients with cartilage grafts, as well as those with rheumatoid arthritis and osteoarthritis. Some of these antibodies react with type II collagen, others are directed against other proteins i.e. anchorin CII and CH65. the role of these diverse molecules, which are present in cartilage cells and separated from the immune system by the matrix, remains unclear.
RESUMO
Fibroblast growth factor 2 (FGF2) inhibits proliferation and hypertrophy of chondrocytes in the growth plate, synthesis of cartilage matrix, terminal differentiation of hypertrophic chondrocytes and matrix calcification. Recent studies have found that mutations in the receptor for fibroblast growth factor 3 (FGFR3) cause achondroplasia, hypochondroplasia and thanatophoric dysplasia. These mutations evoke uncontrolled stimulation of the receptor, leading to inhibition of bone growth. Inactivation of the receptor in experimental animals causes excessive chondrocyte proliferation and abnormal bone length. Chondrocyte stem cells proliferate in the ossification groove of Ranvier and contribute to both peripheral and longitudinal growth of the growth plate. They express FGFR3, have a potential to differentiate into chondrocytes and are therefore considered adequate for healing cartilage defects in the articular surface. It is at present unknown what happens to the chondrocyte precursor cells in the ossification groove of patients with FGFR3 mutation.
Assuntos
Acondroplasia/genética , Fatores de Crescimento de Fibroblastos/genética , Displasia Tanatofórica/genética , Humanos , Mutação Puntual/genéticaRESUMO
Cartilage formed in transplants of allogeneic chondrocytes into joint cartilage defects in rats was infiltrated by immune cells migrating from the bone marrow while the surface on the side of the joint cavity remained free of infiltrations. This suggested that immunization occurred via bone marrow and not via joint cavity. Because articular cartilage is nourished exclusively by the synovial fluid, we have attempted to prevent cartilage rejection by protecting transplants from the contact with bone marrow. Defects in articular surface were filled with bone cement and chondrocytes were transplanted into a cavity prepared within the bone cement plug. Cartilage formed within the cement shell remained free of infiltrations and did not evoke systemic immunological response. However, distribution of glycosaminoglycans in the matrix of protected transplants was irregular. Cultures of chondrocytes growing in vitro on cement contained less glycosaminoglycans than the controls. This suggests that some factor(s) released from the cement unfavorably influenced chondrocytes and matrix production in protected transplants.
Assuntos
Cimentos Ósseos/metabolismo , Cartilagem Articular/anatomia & histologia , Condrócitos/imunologia , Condrócitos/transplante , Animais , Cartilagem Articular/metabolismo , Células Cultivadas , Condrócitos/metabolismo , Testes Imunológicos de Citotoxicidade , Fêmur , Glicosaminoglicanos/metabolismo , Rejeição de Enxerto/prevenção & controle , Articulação do Joelho , Ratos , Ratos Endogâmicos , Ratos Wistar , Estatísticas não ParamétricasRESUMO
Mast and parietal cells were concomitantly demonstrated in biopsies from corpus mucosa of patients with various forms of chronic gastritis. The ratio of mast/parietal cells and the ratio of maximal and basal acid output were negatively correlated.
Assuntos
Ácido Gástrico/metabolismo , Gastrite/patologia , Gastrite/fisiopatologia , Mastócitos/patologia , Células Parietais Gástricas/patologia , Adulto , Idoso , Contagem de Células , Doença Crônica , Feminino , Mucosa Gástrica/efeitos dos fármacos , Mucosa Gástrica/patologia , Mucosa Gástrica/fisiopatologia , Histamina/fisiologia , Humanos , Masculino , Pessoa de Meia-Idade , Pentagastrina/farmacologiaRESUMO
The Golgi complex of mammalian cells is composed of cisternal stacks that function in processing and sorting of membrane and luminal proteins during transport from the site of synthesis in the endoplasmic reticulum to lysosomes, secretory vacuoles, and the cell surface. Even though exceptions are found, the Golgi stacks are usually arranged as an interconnected network in the region around the centrosome, the major organizing center for cytoplasmic microtubules. A close relation thus exists between Golgi elements and microtubules (especially the stable subpopulation enriched in detyrosinated and acetylated tubulin). After drug-induced disruption of microtubules, the Golgi stacks are disconnected from each other, partly broken up, dispersed in the cytoplasm, and redistributed to endoplasmic reticulum exit sites. Despite this, intracellular protein traffic is only moderately disturbed. Following removal of the drugs, scattered Golgi elements move along reassembling microtubules back to the centrosomal region and reunite into a continuous system. The microtubule-dependent motor proteins cytoplasmic dynein and kinesin bind to Golgi membranes and have been implicated in vesicular transport to and from the Golgi complex. Microinjection of dynein heavy chain antibodies causes dispersal of the Golgi complex, and the Golgi complex of cells lacking cytoplasmic dynein is likewise spread throughout the cytoplasm. In a similar manner, kinesin antibodies have been found to inhibit Golgi-to-endoplasmic reticulum transport in brefeldin A-treated cells and scattering of Golgi elements along remaining microtubules in cells exposed to a low concentration of nocodazole. The molecular mechanisms in the interaction between microtubules and membranes are, however, incompletely understood. During mitosis, the Golgi complex is extensively reorganized in order to ensure an equal partitioning of this single-copy organelle between the daughter cells. Mitosis-promoting factor, a complex of cdc2 kinase and cyclin B, is a key regulator of this and other events in the induction of cell division. Cytoplasmic microtubules depolymerize in prophase and as a result thereof, the Golgi stacks become smaller, disengage from each other, and take up a perinuclear distribution. The mitotic spindle is thereafter put together, aligns the chromosomes in the metaphase plate, and eventually pulls the sister chromatids apart in anaphase. In parallel, the Golgi stacks are broken down into clusters of vesicles and tubules and movement of protein along the exocytic and endocytic pathways is inhibited. Using a cell-free system, it has been established that the fragmentation of the Golgi stacks is due to a continued budding of transport vesicles and a concomitant inhibition of the fusion of the vesicles with their target membranes. In telophase and after cytokinesis, a Golgi complex made up of interconnected cisternal stacks is recreated in each daughter cell and intracellular protein traffic is resumed. This restoration of a normal interphase morphology and function is dependent on reassembly of a radiating array of cytoplasmic microtubules along which vesicles can be carried and on reactivation of the machinery for membrane fusion.
Assuntos
Complexo de Golgi/fisiologia , Microtúbulos/fisiologia , Animais , Citoplasma , Mitose/fisiologiaRESUMO
Rat syngeneic and allogeneic chondrocytes were transplanted intramuscularly or into defects prepared in articular cartilage (intracartilaginous transplants). Recipients of allogeneic transplants received cyclosporin A (CsA), cladribine (2-chlorodeoxyadenosine, 2-CdA), or both drugs in combination. Transplants were taken for examination after 5 weeks. Cartilage formed intramuscularly by syngeneic chondrocytes was ossified. Allogeneic cartilage was resorbed by infiltrating cells. CsA or 2-CdA partially suppressed, and both these agents in combination strongly suppressed, formation of infiltrations. Both syngeneic and allogeneic chondrocytes formed cartilage in joint surface defects but only allogeneic cartilage was attacked by infiltrating cells. CsA + 2-CdA treatment slightly decreased intensity of infiltrations but did not prevent cartilage resorption. Antichondrocyte response was studied by evaluation of spleen mononuclear cells (SMC) stimulation in mixed splenocyte-chondrocyte cultures and by detection of antichondrocyte cytotoxic antibodies. SMC stimulation index (SI) was calculated separately for syngeneic and allogeneic chondrocytes. Comparison of SMC SI for syngeneic and allogeneic chondrocytes indicated lack of stimulation of SMC from control or syngeneic transplant recipients and significant stimulation of SMC from recipients of allogeneic transplants. SMC from animals treated with CsA + 2-CdA were not stimulated. Additional experiments aiming at an explanation of the lack of stimulation of SMC from intact animals by syngeneic chondrocytes reported in this work and contrary to other findings disclosed that it was caused by the use of collagenase solution containing N alpha-p-tosyl-l-lysine chloromethyl ketone for chondrocyte isolation. Spontaneous antichondrocyte cytotoxic antibody activity was found in intact rats raised only in sera from recipients of allogeneic intramuscular transplants without immunosuppression. Thus, strong immunosuppressive treatment of rats with allogeneic chondrocyte transplants was more effective in relation to the general immunological response than to the local reaction.
Assuntos
Cartilagem Articular/imunologia , Cartilagem Articular/cirurgia , Condrócitos/imunologia , Condrócitos/transplante , Animais , Cartilagem Articular/patologia , Cladribina/farmacologia , Ciclosporina/farmacologia , Citotoxicidade Imunológica , Rejeição de Enxerto , Terapia de Imunossupressão , Imunossupressores/farmacologia , Músculo Esquelético/imunologia , Músculo Esquelético/patologia , Músculo Esquelético/cirurgia , Ratos , Ratos Endogâmicos Lew , Ratos Wistar , Transplante Homólogo , Transplante IsogênicoRESUMO
During mitosis, not only the genetic material stored in the nucleus but also the constituents of the cytoplasm should be equally partitioned between the daughter cells. For this sake, the dividing cell goes through an extensive structural reorganization and transport along the endocytic and exocytic pathways is temporarily arrested. Early in prophase, the radiating array of cytoplasmic microtubules disassembles and the membrane systems of the secretory apparatus start to split up. In metaphase, the nuclear envelope fragments and the condensing chromosomes associate with the forming mitotic spindle. The cisternal and tubular elements of the endoplasmic reticulum and the Golgi complex break down into small vesicles, presumably as the result of an imbalance between vesicle budding and fusion. In anaphase, the two sets of chromosomes are pulled apart and a cleavage furrow forms halfway between the spindle poles. Since most organelles occur in multiple and widely dispersed copies at this stage, they will be evenly distributed between the daughter cells. During telophase and cytokinesis, the preceding fragmentation process is reversed. A nuclear envelope reappears around the chromosomes and cytoplasmic microtubules reassemble. The endoplasmic reticulum is rebuilt as a continuous system of flattened cisternae and tubules. Stacks of Golgi cisternae arise from small vesicles and are rearranged in an interconnected network. In parallel, the biosynthetic functions of the cell are normalized and intracellular membrane traffic is resumed.
Assuntos
Compartimento Celular , Complexo de Golgi/ultraestrutura , Mitose/fisiologia , Organelas/ultraestrutura , Centrossomo/fisiologia , Retículo Endoplasmático/fisiologia , Retículo Endoplasmático/ultraestrutura , Complexo de Golgi/metabolismo , Complexo de Golgi/fisiologia , Microscopia Eletrônica , Microscopia de Fluorescência , Microtúbulos/fisiologia , Microtúbulos/ultraestrutura , Organelas/fisiologiaRESUMO
Rat chondrocytes isolated from the articular-epiphyseal cartilage complex were transplanted into defects prepared in articular cartilage and subchondral bone. Transplants were taken for examination after 3 and 8 wk. Cartilage formed by syngeneic chondrocytes did not evoke formation of infiltrations. Contrary to that, in the vicinity of cartilage produced by allogeneic chondrocytes numerous infiltrating cells were present and cartilage resorption could be observed. Cyclosporine-A (CsA) treatment of recipients of allogeneic chondrocytes only partially suppressed accumulation of infiltrating cells and matrix resorption. Antichondrocyte immune response of chondrocyte graft recipients was studied by evaluation of spleen mononuclear cells (SMC) stimulation in mixed splenocyte-chondrocyte cultures and by evaluation of antichondrocyte cytotoxic antibodies. No difference in stimulation of SMC from intact rats by syngeneic and allogeneic chondrocytes was observed. Stimulation by allogeneic chondrocytes was slightly but significantly higher in recipients of syngeneic grafts. SMC of allogenic chondrocyte recipients were strongly stimulated by allogeneic chondrocytes. This response was absent in recipients treated with CsA. Spontaneous antichondrocyte cytotoxic antibody activity was detected in intact rats and in recipients of syngeneic grafts. In recipients of allogeneic chondrocytes the antibody response against allogeneic chondrocytes was raised but was statistically not significant owing to the considerable variation in the level of spontaneously occurring antichondrocyte antibodies.
Assuntos
Cartilagem Articular/citologia , Transplante de Células , Ciclosporina/uso terapêutico , Epífises/citologia , Artropatias/cirurgia , Transplante Homólogo/imunologia , Animais , Matriz Óssea , Reabsorção Óssea , Teste de Cultura Mista de Linfócitos , Necrose , Ratos , Ratos Endogâmicos Lew , Ratos Wistar , Baço/imunologia , Transplante Homólogo/patologiaRESUMO
The beginning of pancreatic islet isolation by collagenase digestion is described in the form of personal account.
Assuntos
Separação Celular/métodos , Ilhotas Pancreáticas/citologia , Animais , Separação Celular/história , Células Cultivadas , Colagenases , História do Século XX , Humanos , Insulina/metabolismo , Secreção de Insulina , PolôniaRESUMO
A simple technique for concomitant staining of mast and parietal cells in the same section is described. Mast cells were stained by alcian blue or astra blue in methanol-formalin-acetic acid fixed biopsies of gastric mucosa. Parietal cells were visualized by Dolichos biflorus lectin binding.
Assuntos
Mucosa Gástrica/citologia , Mastócitos/citologia , Células Parietais Gástricas/citologia , Lectinas de Plantas , Coloração e Rotulagem/métodos , Azul Alciano/química , Humanos , Indóis/química , Lectinas/químicaRESUMO
To study the possible effect of collagen cross-link formation on the lamellar bone deposition, isolated mouse bone cells were transplanted in the syngeneic system and the formation of bone was followed in control and beta-aminopropionitrile (BAPN) treated animals. Woven and lamellar bone were distinguished by shape of bone lacunae, PAS reaction and Sirius red staining of collagen fibers. BAPN was administered in various doses either subcutaneously or in drinking water. Bone formed in BAPN treated animals contained both woven and lamellar bone in similar proportion as in controls. Thus, cross-linking of collagen seems to be unnecessary for lamellar bone deposition.
Assuntos
Aminopropionitrilo/farmacologia , Desenvolvimento Ósseo/efeitos dos fármacos , Animais , Osso e Ossos/citologia , Osso e Ossos/efeitos dos fármacos , Osso e Ossos/fisiologia , Colágeno/metabolismo , Elastina/metabolismo , Feminino , Masculino , Camundongos , Camundongos EndogâmicosRESUMO
Cellular infiltrates participating in rejection of cartilage formed by transplanted allogeneic rat epiphyseal chondrocytes were evaluated immunohistochemically using a panel of different monoclonal antibodies. One week after transplantation, the grafts were surrounded by numerous class II MHC+ (OX6+, OX17+), CD4+ (W3/25+), and W3/13+ cells as well as some ED1+ monocytes/macrophages. Only a few T (OX19+) and B (HIS14+) cells were present. The number of class II MHC+ cells and ED1+ monocytes/macrophages did not change significantly in the course of rejection whereas the number of CD4+ and W3/13+ cells gradually decreased. On the other hand, there was a significant increase in the number of CD8+ (OX8+) cells. CD8+ cells accumulated close to the transplants and some of them penetrated cartilage matrix suggesting that they might be involved in chondrocyte killing. After 3 months, cartilage was almost completely destroyed and the intensity of infiltrations was markedly decreased. Fibrous connective tissue predominated, however, some class II+ as well as few ED1+, CD4+ and CD8+ cells were still present adjacent to the cartilage remnants. At the time of transplantation, chondrocytes were endowed with RT1.D class II antigen (OX17+), but they did not react with OX6 mAb (monoclonal antibody) recognizing the RT1.B class II molecule. However, after 1 week, some chondrocytes reacted with OX6 mAb and the number of RT1.B positive chondrocytes increased in the course of cartilage rejection.
Assuntos
Anticorpos Monoclonais , Cartilagem/transplante , Rejeição de Enxerto/imunologia , Animais , Cartilagem/citologia , Cartilagem/imunologia , Movimento Celular/imunologia , Rejeição de Enxerto/patologia , Antígenos de Histocompatibilidade Classe II/análise , Ratos , Ratos Wistar , Transplante HomólogoRESUMO
The eye irritancy test in rabbits (Draize test) is currently the method used to evaluate the hazard or safety of chemical substances. To reduce the need for animal testing some new procedures as alternative were elaborated. We present a review of method used as well as evaluation of sensitivity and repeatability of alternative tests applied in laboratories of European Economic Communities.
Assuntos
Alternativas aos Testes com Animais , Olho/efeitos dos fármacos , Substâncias Perigosas/toxicidade , Irritantes/toxicidade , Animais , Coelhos , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
The Golgi complex of mammalian cells is disorganized into dispersed vesicular and tubular elements during mitosis and is then reorganized into an interconnected system of cisternal stacks in each daughter cell during cytokinesis. Recent studies further indicate that the Golgi complex is typically relocated from the proximal to the distal side of the nucleus in the terminal phase of cytokinesis (as related to the intercellular bridge). Here, the functional role of this shift in position was approached using rat embryo fibroblasts synchronized with thymidine and nocodazole. Mitotic cells were collected by shaking and seeded in medium without or with brefeldin A (a fungal metabolite that inhibits protein secretion). They were fixed after one or two hours and stained for immunocytochemical demonstration of mannosidase II (a Golgi protein), fibronectin (an extracellular matrix protein), the fibronectin receptor (a member of the integrin family of proteins), and connexin 43 (a member of the connexin family of gap junction proteins). One hour after seeding, the cells had completed mitosis and progressed into cytokinesis. The Golgi complex was now usually located on the proximal side of the nucleus and overlapping fibrillar arrays of fibronectin and fibronectin receptors were observed in the contact zone between the daughter cells, while connexin 43 mainly occurred in fine dispersed spots. Two hours after seeding, the cells had spread out on the substrate and started to move apart. The Golgi complex was now usually located on the distal side of the nucleus. Moreover, fibronectin and fibronectin receptors were found to codistribute both in the contact zone between the daughter cells and in adhesive contacts beneath them, while connexin 43 was concentrated to plaques in the former zone. After treatment with brefeldin A, there was a diffuse cytoplasmic staining for mannosidase II and fibronectin and no distinct extracellular staining for fibronectin was noted. In addition, the connexin 43 positive plaques were reduced in size and number. Although the cells completed cytokinesis in the presence of the drug, they showed an increased tendency to detach from the substrate and locate on top of each other rather than to move apart normally. Taken together, the observations suggest that the change in position of the Golgi complex during cytokinesis serves the function to direct transport of secretory proteins as well as membrane constituents to different parts of the cell surface at different times.(ABSTRACT TRUNCATED AT 400 WORDS)
Assuntos
Divisão Celular/fisiologia , Complexo de Golgi/fisiologia , Animais , Brefeldina A , Adesão Celular/fisiologia , Comunicação Celular/fisiologia , Divisão Celular/efeitos dos fármacos , Células Cultivadas , Ciclopentanos/farmacologia , Matriz Extracelular/fisiologia , Matriz Extracelular/ultraestrutura , Fibronectinas/metabolismo , Imuno-Histoquímica , Manosidases/metabolismo , Ratos , Receptores de Fibronectina/metabolismo , Vinculina/metabolismoRESUMO
Rat jejunum was fixed with either formalin or methanol-formalin acetic acid (MFAA) and stained with Astra Blue or Alcian Blue with or without microwave irradiation. Staining of both mucosal mast cells and granulated intra-epithelial lymphocytes after formalin fixation was considerably improved by microwave irradiation. On the other hand, microwave irradiation slightly impaired staining of mucosal mast cells (MMC) and even more strongly granulated intra-epithelial lymphocytes (GIEL) after MFAA fixation.
Assuntos
Mucosa Intestinal/citologia , Linfócitos/citologia , Mastócitos/citologia , Micro-Ondas , Coloração e Rotulagem/métodos , Acetatos , Ácido Acético , Azul Alciano , Animais , Formaldeído , Humanos , Indóis , Jejuno/citologia , Metanol , Ratos , Ratos Wistar , Fixação de TecidosRESUMO
Syngeneic rat chondrocytes isolated from the articular-epiphyseal cartilage complex were suspended in hyaluronic acid and transplanted intramuscularly or into joint surface defects. Transplants were fixed in ruthenium hexammonium trichloride and embedded in glycol methacrylate. In cartilage nodules produced intramuscularly, chondrocyte hypertrophy and matrix calcification were observed after 2 wk. Partial ossification occurred after 4 wk and the cartilage was almost completely replaced by an ossicle after 8 wk. Only small, dispersed groups of chondrocytes remained within the ossicle. In cartilage formed in joint surface defects a superficial and a deep zone were distinguished. Chondrocytes in the superficial zone did not hypertrophy and cartilage remained unossified. In the deep zone matrix calcification and bone formation occurred. These processes were, however, retarded in comparison with intramuscular transplants. Thus, either intraarticular environment exerted an inhibitory effect on chondrocyte hypertrophy and matrix calcification or articular chondrocytes present among transplanted cells accumulated close to the joint lumen and reconstructed normal articular cartilage.