RESUMO
Hepatic fibropoiesis in canine visceral leishmaniasis (CVL) were evaluated by histological (morphometrical collagen deposition) and immunohistochemical assays characterizing alpha-actin (α-SMA), vimentin, calprotectin (L1 antigen), and TGF-ß in 46 naturally infected dogs with Leishmania infantum treated with liposome-encapsulated meglumine antimoniate and allopurinol separately and in combination. Six treatment groups were defined: meglumine antimoniate encapsulated in nanometric liposomes (LMA), allopurinol (ALLOP); liposome-encapsulated meglumine antomoniate combined with allopurinol (LMA+ALLOP); empty liposomes (LEMP); empty liposomes combined with allopurinol (LEMP+ALLOP) and saline. Relative liver weight was lower in LMA, LMA+ALLOP, and ALLOP groups compared to the LEMP control. Significantly lower granulomatous chronic inflammatory reaction was seen in the ALLOP group compared to a control group. Calprotectin was lowest in liver of those dogs showing lower numbers of intralobular hepatic granulomas. Collagen deposits were significantly higher in LMA compared to ALLOP, LEMP+ALLOP, and Saline groups. LMA+ALLOP group collagen deposition was higher than dogs treated only with allopurinol. Immunohistochemical analysis showed significant higher α-SMA in hepatic stellate cells (HSCs), hepatic perisinusoidal cells, in control groups than LMA+ALLOP and LEMP+ALLOP. Alpha-actin and Vimentin positive cells were diffusely distributed throughout the liver parenchyma in the hepatic lobule, mainly in HSCs. Vimentin expression was significantly higher in the saline group than in the ALLOP group. Our data suggest that allopurinol inhibits HSC and results in lower collagen deposits in liver during CVL progression, as supported by the significantly lower expression of TGF-ß in the ALLOP group compared to other groups. Results demonstrated that treatment with allopurinol inhibited chronic granulomatous inflammatory reaction and hepatic fibrosis in CVL.
Assuntos
Alopurinol/uso terapêutico , Doenças do Cão/tratamento farmacológico , Leishmaniose Visceral/veterinária , Cirrose Hepática/veterinária , Meglumina/uso terapêutico , Compostos Organometálicos/uso terapêutico , Alopurinol/farmacologia , Animais , Antiprotozoários/farmacologia , Antiprotozoários/uso terapêutico , Cães , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Leishmania infantum , Leishmaniose Visceral/complicações , Leishmaniose Visceral/tratamento farmacológico , Lipossomos/administração & dosagem , Fígado/efeitos dos fármacos , Cirrose Hepática/etiologia , Masculino , Meglumina/farmacologia , Antimoniato de Meglumina , Compostos Organometálicos/farmacologia , Distribuição Aleatória , Fator de Crescimento Transformador beta/genética , Vimentina/genéticaRESUMO
Neutrophils are involved in the early stages of immune responses to pathogens. Here, we investigated the role of neutrophils during the establishment of Leishmania amazonensis infection in BALB/c and C57BL/6 mice. First, we showed an accumulation of neutrophils between 6 and 24 h post-infection, followed by a reduction in neutrophil numbers after 72 h. Next, we depleted neutrophils prior to infection using RB6-8C5 or 1A8 mAb. Neutrophil depletion led to faster lesion development, increased parasite numbers and higher arginase activity during the first week of infection in BALB/c mice, but not in C57BL/6 mice. Increased susceptibility was accompanied by augmented levels of anti-L. amazonensis IgG and increased production of IL-10 and IL-17. Because IL-10 is a mediator of susceptibility to Leishmania infection, we blocked IL-10 signalling in neutrophil-depleted mice using anti-IL-10R. Interestingly, inhibition of IL-10 signalling abrogated the increase in parasite loads observed in neutrophil-depleted mice, suggesting that parasite proliferation is at least partially mediated by IL-10. Additionally, we tested the effect of IL-17 in inflammatory macrophages and observed that IL-17 increased arginase activity and favoured parasite growth. Taken together, our data indicate that neutrophils control parasite numbers and limit lesion development during the first week of infection in BALB/c mice.
Assuntos
Leishmania mexicana/crescimento & desenvolvimento , Leishmania mexicana/imunologia , Leishmaniose Cutânea/imunologia , Leishmaniose Cutânea/parasitologia , Neutrófilos/imunologia , Animais , Anticorpos Antiprotozoários/sangue , Arginase/metabolismo , Feminino , Imunoglobulina G/sangue , Interleucina-10/metabolismo , Interleucina-17/metabolismo , Cinética , Ativação de Macrófagos , Macrófagos/imunologia , Macrófagos/metabolismo , Macrófagos/parasitologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Infiltração de Neutrófilos , Carga Parasitária , Linfócitos T Reguladores/imunologiaRESUMO
Macrophages are cells that function as a first line of defence against invading microorganisms. One of the hallmarks of macrophages is their ability to become activated in response to exogenous 'danger signals'. Most microbes have molecular patterns (PAMPS) that are recognized by macrophages and trigger this activation response. There are many aspects of the activation response to PAMPS that are recapitulated when macrophages encounter endogenous danger signals. In response to damaged or stressed self, macrophages undergo physiological changes that include the initiation of signal transduction cascades from germline-encoded receptors, resulting in the elaboration of chemokines, cytokines and toxic mediators. This response to endogenous mediators can enhance inflammation, and thereby contribute to autoimmune pathologies. Often the overall inflammatory response is the result of cooperative activation signals from both exogenous and endogenous signals. Macrophage activation plays a critical role, not only in the initiation of the inflammatory response but also in the resolution of this response. The clearance of granulocytes and the elaboration of anti-inflammatory mediators by macrophages contribute to the dissolution of the inflammatory response. Thus, macrophages are a key player in the initiation, propagation and resolution of inflammation. This review summarizes our understanding of the role of macrophages in inflammation. We pay particular attention to the endogenous danger signals that macrophages may encounter and the responses that these signals induce. The molecular mechanisms responsible for these responses and the diseases that result from inappropriately controlled macrophage activation are also examined.
Assuntos
Inflamação/imunologia , Ativação de Macrófagos/imunologia , Humanos , Doenças Inflamatórias Intestinais/imunologia , Macrófagos/imunologia , Neoplasias/imunologia , Receptores de Reconhecimento de Padrão/análise , Transdução de Sinais/imunologiaRESUMO
The genetic background of mice infected with Leishmania major determines the response to infection, resulting in a resistant or susceptible phenotype. Susceptible mice develop a T-helper type 2 (Th2)-type immune response following infection distinguished by the development of interleukin (IL)-4 secreting T cells in the lymph node and spleen. In SJL mice, which normally heal L. major lesions, subtoxic doses of mercury induce an autoimmune syndrome characterized by an expansion of Th2 cells. In this study, we examined the effect of mercury administration on the outcome of L. major infection in SJL mice. We show that subtoxic doses of mercuric chloride (HgCl2) exacerbate disease outcome in SJL mice resulting in increased footpad swelling and increased parasite burdens. Furthermore, the effects of HgCl2 treatment on resistance to L. major are time-dependent. The nonhealing phenotype was observed only if mice had been treated with HgCl2 prior to L. major infection for at least 1 week, a timepoint at which mice treated with HgCl2 alone had increased splenocyte IL-4 production. HgCl2 treatment also increased production of serum immunoglobulin (Ig)E and IgG1, two IL-4 dependent isotypes. These results show that HgCl2 treatment enhances the susceptibility to L. major in SJL mice, consistent with the induction of host Th2 parameters. These findings have implications for the role of mercury contamination in areas of endemic leishmaniasis.
Assuntos
Leishmania major/imunologia , Leishmaniose Cutânea/imunologia , Cloreto de Mercúrio/administração & dosagem , Animais , Modelos Animais de Doenças , Suscetibilidade a Doenças , Feminino , Imunoglobulina E/sangue , Imunoglobulina G/sangue , Interleucina-4/biossíntese , Leishmania major/patogenicidade , Leishmaniose Cutânea/parasitologia , Cloreto de Mercúrio/farmacologia , Camundongos , Células Th2/imunologiaRESUMO
Our laboratory has previously demonstrated that the ligation of phagocytic receptors on macrophages can influence cytokine production. In this study, we examine the cytokine responses to multiple inflammatory stimuli following FcgammaR ligation. Macrophages were stimulated in vitro with LPS, lipoteichoic acid, CD40 ligand, or low molecular mass hyaluronic acid. All of these stimuli were proinflammatory in character, inducing the production of high levels of IL-12, but only modest amounts of IL-10. The coligation of FcgammaR along with these stimuli resulted in an anti-inflammatory profile, abrogating IL-12 production and inducing high levels of IL-10. The modulation of these two cytokines occurred by two independent mechanisms. Whereas the abrogation of IL-12 biosynthesis was a property shared by several macrophage receptors, the induction of IL-10 was specific to the FcgammaR. The biological relevance of these observations was examined in murine models of endotoxemia, in which FcgammaR ligation induced the rapid production of IL-10 and prevented IL-12 synthesis. Mice could be passively immunized with Abs to LPS to reverse inflammatory cytokine production, and the transfer of macrophages whose FcgammaR had been ligated could rescue mice from lethal endotoxemia. Thus, the ligation of the macrophage FcgammaR can be exploited to prevent inappropriate inflammatory cytokine responses.
Assuntos
Lipopolissacarídeos/antagonistas & inibidores , Lipopolissacarídeos/toxicidade , Macrófagos/imunologia , Macrófagos/metabolismo , Receptores de IgG/metabolismo , Animais , Células da Medula Óssea/imunologia , Células da Medula Óssea/metabolismo , Transplante de Medula Óssea , Células Cultivadas , Complemento C3b/metabolismo , Citocinas/biossíntese , Citocinas/sangue , Regulação para Baixo/imunologia , Eritrócitos/imunologia , Feminino , Imunização Passiva , Imunoglobulina G/administração & dosagem , Imunoglobulina G/sangue , Injeções Intraperitoneais , Injeções Intravenosas , Interleucina-10/biossíntese , Interleucina-12/antagonistas & inibidores , Interleucina-12/biossíntese , Cinética , Ligantes , Lipopolissacarídeos/administração & dosagem , Lipopolissacarídeos/imunologia , Macrófagos/transplante , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Proteínas Opsonizantes/sangue , Receptores de IgG/fisiologiaRESUMO
CD40 ligand (CD40L) is a potent inducer of interleukin-12 (IL-12) production from macrophages and dendritic cells. We show that combining CD40L with antigen derived from Leishmania is an effective way to preferentially induce type 1 immune responses to the antigen and to vaccinate mice against subsequent challenge with virulent organisms. Mice vaccinated in this way had smaller lesions, with more than 1,000-fold fewer parasites within them. To improve the efficiency of CD40L-induced immunopotentiation, we attempted to specifically direct CD40L to macrophages. We developed transfected cells expressing CD40L and a single Leishmania antigen, gp63. These cells bound efficiently to macrophages and induced robust IL-12 production. Vaccination with these cotransfected cells provided a significant degree of protection against challenge with virulent organisms. CD40L was also adsorbed to the surface of virulent Leishmania. These organisms induced only modest lesions in genetically susceptible mice, and the lesions had an average of 10(5)-fold fewer organisms within them relative to control mice. These studies suggest that CD40L could be exploited to improve vaccines against intracellular pathogens, especially those organisms that reside within cells expressing CD40 on their surface.
Assuntos
Ligante de CD40/imunologia , Leishmania major/imunologia , Leishmania mexicana/imunologia , Macrófagos/fisiologia , Vacinas Protozoárias/imunologia , Animais , Feminino , Interferon gama/biossíntese , Interleucina-2/biossíntese , Interleucina-4/biossíntese , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , VacinaçãoRESUMO
Ligating Fc gamma R on macrophages results in suppression of IL-12 production. We show that Fc gamma R ligation selectively down-regulates IL-12 p40 and p35 gene expression at the level of transcription. The region responsive to this inhibition maps to the Ets site of the p40 promoter. PU.1, IFN consensus sequence binding protein, and c-REL: form a complex on this element upon macrophage activation. Receptor ligation abolishes the binding of this PU.1-containing activation complex, and abrogates p40 transcription. A dominant-negative construct of PU.1 diminishes IL-12 p40 promoter activity and endogenous IL-12 p40 protein secretion. Thus, the specificity of IL-12 down-regulation following receptor ligation lies in the inhibition of binding of a PU.1-containing complex to the Ets site of the IL-12 promoter. These findings provide evidence demonstrating for the first time the importance of PU.1 in the transcriptional regulation of IL-12 gene expression.
Assuntos
Regulação para Baixo/genética , Regulação para Baixo/imunologia , Interleucina-12/antagonistas & inibidores , Interleucina-12/genética , Macrófagos/metabolismo , Receptores de IgG/imunologia , Receptores de IgG/metabolismo , Fatores de Transcrição , Transcrição Gênica/imunologia , Transporte Ativo do Núcleo Celular/imunologia , Animais , Ligação Competitiva/genética , Ligação Competitiva/imunologia , Linhagem Celular , Núcleo Celular/imunologia , Núcleo Celular/metabolismo , Proteínas de Ligação a DNA/antagonistas & inibidores , Proteínas de Ligação a DNA/metabolismo , Regulação da Expressão Gênica/imunologia , Vetores Genéticos/farmacologia , Fator Regulador 1 de Interferon , Fatores Reguladores de Interferon , Interferon gama/metabolismo , Interleucina-12/biossíntese , Interleucina-12/metabolismo , Ligantes , Substâncias Macromoleculares , Macrófagos/imunologia , Camundongos , Fosfoproteínas/antagonistas & inibidores , Fosfoproteínas/metabolismo , Regiões Promotoras Genéticas/imunologia , Ligação Proteica/genética , Ligação Proteica/imunologia , Estrutura Terciária de Proteína/genética , Proteínas Proto-Oncogênicas/antagonistas & inibidores , Proteínas Proto-Oncogênicas/genética , Proteínas Proto-Oncogênicas/metabolismo , Proteínas Proto-Oncogênicas/fisiologia , Proteínas Repressoras/antagonistas & inibidores , Proteínas Repressoras/metabolismo , Transativadores/antagonistas & inibidores , Transativadores/genética , Transativadores/metabolismo , Transativadores/fisiologia , TransfecçãoRESUMO
The macrophage receptors for the Fc portion of immunoglobulin G (FcgammaR) have long been known to mediate a variety of effector functions that are vital to the adaptive immune response. Recent studies, however, have begun to stress potential regulatory roles that these receptors can play in modulating immune and inflammatory responses. In this article we discuss the activating and inhibitory properties of the individual macrophage FcgammaR and the conditions under which these heterologous responses can occur.
Assuntos
Imunoglobulina G/metabolismo , Macrófagos/metabolismo , Receptores de IgG/metabolismo , Transdução de Sinais , Animais , Humanos , Interleucina-10/biossíntese , Macrófagos/imunologia , CamundongosRESUMO
To determine the role of IL-10 in cutaneous leishmaniasis, we examined lesion development following Leishmania major infection of genetically susceptible BALB/c mice lacking IL-10. Whereas normal BALB/c mice developed progressive nonhealing lesions with numerous parasites within them, IL-10(-/-) BALB/c mice controlled disease progression, and had relatively small lesions with 1000-fold fewer parasites within them by the fifth week of infection. We also examined a mechanism whereby Leishmania induced the production of IL-10 from macrophages. We show that surface IgG on Leishmania amastigotes allows them to ligate Fc gamma receptors on inflammatory macrophages to preferentially induce the production of high amounts of IL-10. The IL-10 produced by infected macrophages prevented macrophage activation and diminished their production of IL-12 and TNF-alpha. In vitro survival assays confirmed the importance of IL-10 in preventing parasite killing by activated macrophages. Pretreatment of monolayers with either rIL-10 or supernatants from amastigote-infected macrophages resulted in a dramatic enhancement in parasite intracellular survival. These studies indicate that amastigotes of Leishmania use an unusual and unexpected virulence factor, host IgG. This IgG allows amastigotes to exploit the antiinflammatory effects of Fc gamma R ligation to induce the production of IL-10, which renders macrophages refractory to the activating effects of IFN-gamma.
Assuntos
Interleucina-10/fisiologia , Leishmania major/imunologia , Leishmaniose Cutânea/imunologia , Leishmaniose Cutânea/parasitologia , Adjuvantes Imunológicos/fisiologia , Animais , Sistema Livre de Células/imunologia , Sistema Livre de Células/parasitologia , Progressão da Doença , Imunoglobulina G/metabolismo , Interferon gama/antagonistas & inibidores , Interferon gama/farmacologia , Interleucina-10/biossíntese , Interleucina-10/deficiência , Interleucina-10/genética , Interleucina-12/antagonistas & inibidores , Interleucina-12/biossíntese , Leishmania major/crescimento & desenvolvimento , Leishmaniose Cutânea/etiologia , Leishmaniose Cutânea/genética , Lipopolissacarídeos/antagonistas & inibidores , Lipopolissacarídeos/farmacologia , Ativação de Macrófagos/imunologia , Macrófagos/imunologia , Macrófagos/metabolismo , Macrófagos/parasitologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C3H , Camundongos Endogâmicos C57BL , Camundongos Knockout , Receptores de Antígenos de Linfócitos B/metabolismo , Fator de Necrose Tumoral alfa/antagonistas & inibidores , Fator de Necrose Tumoral alfa/biossínteseRESUMO
In leishmaniasis, macrophages play important but potentially divergent roles. They act as the host cell in which the parasite may reside and replicate, and, at the same time, they act as an effector cell with the potential to eliminate the parasite. In this work, we experimentally induced an inflammatory model that provokes a continued recruitment of the monocytes to the site of inflammation. This model was carried out by means of implanting paraffin tablets under the skin of Balb/c or C57BL/6 mice. Mice were then infected with Leishmania major to determine how the monocyte inflammatory response to paraffin could influence the course of infection with L. major. Mice were sacrificed 15, 21, 30, and 45 days after infection, and skin and inflammatory capsule were collected for histopathology. At 15 days and 21 days, the lesions induced by L. major in combination with paraffin contained markedly increased numbers of parasites relative to lesions in parallel control animals infected with L. major (without paraffin). Both Balb/c and C57BL/6 mice exhibited high parasite numbers in their lesions. The intense parasite burden observed following paraffin implantation would suggest that the monocytes-macrophages that are recruited to the lesion are acting more as a host cell permitting parasite growth than as an effector cell capable of eliminating L. major. At later times, the two strains of mice stratified according to their genetic susceptibility/resistance profiles. Susceptible Balb/c mice continue to have large parasite burdens, whereas the resistant C56BL/6 mice begin to control parasite numbers. This later observation indicates that the genetic difference between susceptible and resistant strains is not due to differences in monocyte recruitment and cannot be reversed through the altering of monocyte inflammation.
Assuntos
Inflamação/etiologia , Leishmania major , Leishmaniose Cutânea/patologia , Animais , Modelos Animais de Doenças , Macrófagos/patologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Monócitos/patologia , ParafinaRESUMO
In this study we investigated the capacity of morphine to modulate expression of cytokines in peritoneal macrophages. Mice were implanted subcutaneously with a 75-mg morphine slow-release pellet, and 48 h later resident peritoneal macrophages were harvested. Control groups received placebo pellets, naltrexone pellets, or morphine plus naltrexone pellets. Adherent cells were stimulated with lipopolysaccharide (LPS: 10 microg/mL) plus interferon-gamma (IFN-gamma: 100 units/mL) to induce cytokine production. After 24 h RNA was extracted for analysis of cytokine mRNA levels by reverse transcriptase-polymerase chain reaction, or supernatants were collected after 48 h for determination of cytokine production by enzyme-linked immunosorbent assay (ELISA). Morphine enhanced mRNA expression of interleukin (IL)-12 p40 and tumor necrosis factor alpha (TNF-alpha) compared with controls, whereas IL-10 levels were unchanged by drug treatment. ELISA data showed that both IL-12 p40 and p70 were increased by morphine. The enhancement of IL-12 at both the mRNA and protein levels was antagonized by naltrexone, indicating that the modulation of this cytokine by morphine is via a classic opioid receptor. These results are particularly interesting in light of our previous observation that 48 h after morphine pellet implantation, the peritoneal cavity is colonized with gram-negative and other enteric bacteria. The enhancement of IL-12 by morphine might be related to morphine-induced sepsis.
Assuntos
Analgésicos Opioides/farmacologia , Interleucina-12/biossíntese , Macrófagos Peritoneais/efeitos dos fármacos , Morfina/farmacologia , Adjuvantes Imunológicos/biossíntese , Adjuvantes Imunológicos/genética , Analgésicos Opioides/antagonistas & inibidores , Animais , Corynebacterium , Preparações de Ação Retardada , Feminino , Mediadores da Inflamação , Interferon gama/farmacologia , Interleucina-10/biossíntese , Interleucina-10/genética , Interleucina-12/genética , Lipopolissacarídeos/farmacologia , Macrófagos Peritoneais/metabolismo , Camundongos , Camundongos Endogâmicos C3H , Morfina/antagonistas & inibidores , Naltrexona/farmacologia , Antagonistas de Entorpecentes/farmacologia , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Estimulação Química , Fator de Necrose Tumoral alfa/biossíntese , Fator de Necrose Tumoral alfa/genéticaRESUMO
High molecular weight kininogen (HK) and its cleaved form (HKa) have been shown to bind to neutrophils. Based on studies using monoclonal antibodies (mAbs), we postulated that CD11b/CD18 (Mac-1) might be the receptor on the neutrophils for binding to HK/HKa. However, the direct interaction of HK/HKa and Mac-1 had not been demonstrated. We therefore transfected HEK 293 cells with human Mac-1. Cell binding assays using fluorescein isothiocyanate-labeled HKa showed increased binding to the Mac-1 transfected cells compared with the control transfected cells. The binding was specific because unlabeled HKa, Mac-1-specific antibody, and fibrinogen can inhibit the binding of biotin-HKa to Mac-1 transfected cells. HKa bound to Mac-1 transfected cells (20 000 molecules/cell) with a K(d) = 62 nmol/L. To demonstrate directly the formation of a complex between HKa and Mac-1, we examined the interaction of HKa and purified Mac-1 in a cell-free system using an IAsys resonant mirror optical biosensor. The association and dissociation rate constants (k(on) and k(off), respectively) were determined, and they yielded a dissociation constant (K(d)) of 3.2x10(-9) mol/L. The functional significance of direct interaction of HKa to Mac-1 was investigated by examining the effect of HKa on cellular adhesion to fibrinogen and intercellular adhesion molecule-1 (ICAM-1), molecules abundant in the injured vessel wall. HKa blocked the adhesion of Mac-1 transfected cells to fibrinogen and ICAM-1 in a dose-dependent manner. Thus, HKa may interrupt Mac-1-mediated cell-extracellular matrix and cell-cell adhesive interactions and may therefore influence the recruitment of circulating neutrophils/monocytes to sites of vessel injury. (Blood. 2000;95:3788-3795)
Assuntos
Adesão Celular/fisiologia , Fibrinogênio/fisiologia , Molécula 1 de Adesão Intercelular/fisiologia , Cininogênios/farmacologia , Antígeno de Macrófago 1/fisiologia , Adesão Celular/efeitos dos fármacos , Linhagem Celular , Fluoresceína-5-Isotiocianato , Humanos , Rim , Cinética , Cininogênios/química , Antígeno de Macrófago 1/efeitos dos fármacos , Antígeno de Macrófago 1/genética , Proteínas Recombinantes/efeitos dos fármacos , Proteínas Recombinantes/metabolismo , TransfecçãoRESUMO
Rhodococcus equi is a facultative intracellular bacterium of macrophages which can infect immunocompromised humans and young horses. In the present study, we examine the mechanism of host defense against R. equi by using a murine model. We show that bacterial killing is dependent upon the presence of gamma interferon (IFN-gamma), which activates macrophages to produce reactive nitrogen and oxygen intermediates. These two radicals combine to form peroxynitrite (ONOO(-)), which kills R. equi. Mice deficient in the production of either the high-output nitric oxide pathway (iNOS(-/-)) or the oxidative burst (gp91(phox-/-)) are more susceptible to lethal R. equi infection and display higher bacterial burdens in their livers, spleens, and lungs than wild-type mice. These in vivo observations, which implicate both nitric oxide (NO) and superoxide (O(2)(-)) in bacterial killing, were reexamined in cell-free radical-generating assays. In these assays, R. equi remains fully viable following prolonged exposure to high concentrations of either nitric oxide or superoxide, indicating that neither compound is sufficient to mediate bacterial killing. In contrast, brief exposure of bacteria to ONOO(-) efficiently kills virulent R. equi. The intracellular killing of bacteria in vitro by activated macrophages correlated with the production of ONOO(-) in situ. Inhibition of nitric oxide production by activated macrophages by using N(G)-monomethyl-L-arginine blocks their production of ONOO(-) and weakens their ability to control rhodococcal replication. These studies indicate that peroxynitrite mediates the intracellular killing of R. equi by IFN-gamma-activated macrophages.
Assuntos
Ativação de Macrófagos/imunologia , Macrófagos/imunologia , Nitratos/metabolismo , Rhodococcus equi/imunologia , Infecções por Actinomycetales/imunologia , Animais , Radicais Livres/metabolismo , Interferon gama/deficiência , Interferon gama/genética , Glicoproteínas de Membrana/deficiência , Glicoproteínas de Membrana/genética , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Mutantes , NADPH Oxidase 2 , NADPH Oxidases/deficiência , NADPH Oxidases/genética , Nitratos/farmacologia , Óxido Nítrico Sintase/deficiência , Óxido Nítrico Sintase/genética , Óxido Nítrico Sintase Tipo II , Óxidos de Nitrogênio/metabolismo , Fagocitose , Espécies Reativas de Oxigênio/metabolismoRESUMO
Leishmania are intracellular protozoan parasites of macrophages. At the cellular level, the disease leishmaniasis involves the invasion of tissue macrophages by the parasite, the avoidance of cellular killing mechanisms, and the subsequent intracellular replication of parasites, with the eventual spread of the organisms to adjacent macrophages. This paper describes the process by which Leishmania organisms invade macrophages, with an overview of some of the molecules involved in this process; the mechanisms available to macrophages that have the potential to restrict the growth of Leishmania within them; and the ways that Leishmania and Leishmania-derived molecules can modulate macrophage functions and circumvent leukocyte antimicrobial responses.
Assuntos
Leishmania/parasitologia , Ativação de Macrófagos/fisiologia , Macrófagos/parasitologia , Animais , Humanos , Leishmania/crescimento & desenvolvimento , Leishmaniose/patologia , Leishmaniose/fisiopatologia , Ligantes , Receptores de Superfície CelularRESUMO
Appropriately regulated immune responses depend on the controlled production of cytokines from antigen presenting cells. IL-12 synthesis is tightly regulated by several redundant mechanisms. One mechanism of IL-12 regulation involves the cross-linking of surface receptors on macrophages. This pathway may be exploited by intracellular pathogens of macrophages to inhibit IL-12 production and delay or prevent the development of cell mediated immunity.
Assuntos
Infecções/imunologia , Interleucina-12/biossíntese , Macrófagos/imunologia , Receptores Imunológicos/fisiologia , Animais , Infecções por HIV/imunologia , Histoplasmose/imunologia , Humanos , Leishmaniose/imunologia , Sarampo/imunologiaRESUMO
The most abundant protein on the surface of the promastigote form of the protozoan parasites Leishmania spp. is a 63-kDa molecule, designated gp63 or leishmanolysin. Because gp63 has been shown to possess fibronectin-like properties, we examined the interaction of gp63 with the cellular receptors for fibronectin. We measured the direct binding of Leishmania to human macrophages or to transfected mammalian cells expressing human fibronectin receptors. Leishmania expressing gp63 exhibited modest but reproducible adhesion to human macrophages and to transfected CHO cells expressing alpha4/beta1 fibronectin receptors. In both cases, this interaction depended on gp63 but occurred independently of the SRYD sequence of gp63, because parasites expressing gp63 with a mutated SRYD sequence bound to macrophages and alpha4/beta1 receptor-expressing cells as well as did wild-type parasites. The contribution of gp63 to parasite adhesion was more pronounced when the assays were performed in the presence of complement, suggesting that the receptors for complement and fibronectin may cooperate to mediate the efficient adhesion of parasites to macrophages. The interaction of gp63 with fibronectin receptors may also play an important role in parasite internalization by macrophages. Erythrocytes to which gp63 was cross-linked were efficiently phagocytized by macrophages, whereas control erythrocytes opsonized with complement alone bound to macrophages but remained peripherally attached to the outside of the cell. Similarly, parasites expressing wild-type gp63 were rapidly and efficiently phagocytized by resting macrophages, whereas parasites lacking gp63 were internalized more slowly. This rapid internalization of gp63-expressing parasites was dependent on the beta1 integrins, because pretreatment of macrophages with monoclonal antibodies to the beta1 integrins decreased the internalization of gp63-expressing parasites. These observations indicate that complement receptors are the primary mediators of parasite adhesion; however, maximal parasite adhesion and internalization may require the participation of the beta1 integrins, which recognize fibronectin-like molecules such as gp63 on the surface of the parasite.
Assuntos
Integrinas/metabolismo , Leishmania/metabolismo , Macrófagos/metabolismo , Metaloendopeptidases/metabolismo , Proteínas de Protozoários/metabolismo , Receptores de Fibronectina/metabolismo , Receptores de Retorno de Linfócitos/metabolismo , Animais , Células CHO , Células Cultivadas , Complemento C3/metabolismo , Cricetinae , Humanos , Integrina alfa4beta1 , Integrinas/genética , Leishmania/imunologia , Macrófagos/citologia , Macrófagos/imunologia , Camundongos , Monócitos/metabolismo , Fagocitose/imunologia , Receptores de Fibronectina/genética , Receptores de Retorno de Linfócitos/genética , Ovinos , TransfecçãoRESUMO
Rhodococcus equi is a facultative intracellular pathogen of macrophages and a cause of pneumonia in young horses (foals) and immunocompromised people. Isolates of R. equi from pneumonic foals typically contain large, 85- or 90-kb plasmids encoding a highly immunogenic virulence-associated protein (VapA). The objective of this study was to determine the role of the 85-kb plasmid and VapA in the intracellular survival and virulence of R. equi. Clinical isolates containing the plasmid and expressing VapA efficiently replicated within mouse macrophages in vitro, while plasmid-cured derivatives of these organisms did not multiply intracellularly. An isolate harboring the large plasmid also replicated in the tissues of experimentally infected mice, whereas its plasmid-cured derivative was rapidly cleared. All foals experimentally infected with a plasmid-containing clinical isolate developed severe bronchopneumonia, whereas the foals infected with its plasmid-cured derivative remained asymptomatic and free of visible lung lesions. By day 14 postinfection, lung bacterial burdens had increased considerably in foals challenged with the plasmid-containing clinical isolate. In contrast, bacteria could no longer be cultured from the lungs of foals challenged with the isogenic plasmid-cured derivative. A recombinant, plasmid-cured derivative expressing wild-type levels of VapA failed to replicate in macrophages and remained avirulent for both mice and foals. These results show that the 85-kb plasmid of R. equi is essential for intracellular replication within macrophages and for development of disease in the native host, the foal. However, expression of VapA alone is not sufficient to restore the virulence phenotype.
Assuntos
Infecções por Actinomycetales/microbiologia , Proteínas de Bactérias/genética , Regulação Bacteriana da Expressão Gênica , Lipoproteínas/genética , Plasmídeos/genética , Rhodococcus equi/fisiologia , Fatores de Virulência , Infecções por Actinomycetales/genética , Infecções por Actinomycetales/patologia , Animais , Humanos , Camundongos , Rhodococcus equi/patogenicidade , Virulência/genéticaRESUMO
Interleukin (IL)-12 is a cytokine that possesses both proinflammatory and immunoregulatory activity. IL-12, and the interferon-gamma (IFN-gamma) that is induced by IL-12, play central roles in the development of the Th1-type immune responses that are required for immunity to intracellular pathogens. Recently a number of these pathogens, including Leishmania, measles virus, and human immunodeficiency virus (HIV), have been shown to subvert the development of cell-mediated immunity by actively inhibiting the production of IL-12. Similarly, the ligation of phagocytic receptors on macrophages has also been shown to suppress IL-12 production. The suppression of IL-12 production by receptor ligation occurs by at least two distinct mechanisms: one involves a direct inhibition of gene transcription and the other depends on the production of inhibitory cytokines. We review studies in which IL-12 has been experimentally manipulated, and we compare the mechanisms by which this regulation can occur. Because the IL-12 that is produced during acute inflammation and chronic autoimmune disorders can lead to exacerbated disease, the development of pharmacological means to suppress IL-12 production is currently under investigation. This review focuses on the production of IL-12 by antigen-presenting cells and the methods by which the down-regulation of IL-12 production can be exploited either by pathogens or for therapeutic ends.
Assuntos
Mediadores da Inflamação/antagonistas & inibidores , Interleucina-12/antagonistas & inibidores , Interleucina-12/biossíntese , Animais , Humanos , Imunossupressores/farmacologia , Interleucina-12/metabolismo , Interleucina-12/fisiologia , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Macrófagos/metabolismo , Receptores de Interleucina/imunologia , Receptores de Interleucina/metabolismo , Receptores de Interleucina-12RESUMO
Macrophages can respond to a variety of infectious and/or inflammatory stimuli by secreting an array of proinflammatory cytokines, the overproduction of which can result in shock or even death. In this report, we demonstrate that ligation of macrophage Fcgamma receptors (FcgammaR) can lead to a reversal of macrophage proinflammatory responses by inducing an upregulation of interleukin (IL)-10, with a reciprocal inhibition of IL-12 production. IL-10 upregulation was specific to FcgammaR ligation, since the ligation of the Mac-1 receptor did not alter IL-10 production. The identification of the specific FcgammaR subtype responsible for IL-10 upregulation was determined in gene knockout mice. Macrophages from mice lacking the FcR gamma chain, which is required for assembly and signaling by FcgammaRI and FcgammaRIII, failed to upregulate IL-10 in response to immune complexes. However, mice lacking either the FcgammaRII or the FcgammaRIII were fully capable of upregulating IL-10 production, implicating FcgammaRI in this process. The biological consequences of FcgammaRI ligation were determined in both in vitro and in vivo models of inflammation and sepsis. In all of the models tested, the ligation of FcgammaR promoted the production of IL-10 and inhibited the secretion of IL-12. This reciprocal alteration in the pattern of macrophage cytokine production illustrates a potentially important role for FcgammaR-mediated clearance in suppressing macrophage proinflammatory responses.
Assuntos
Inflamação/fisiopatologia , Macrófagos/metabolismo , Receptores de IgG/metabolismo , Animais , Citocinas/metabolismo , Interleucina-10/metabolismo , Interleucina-12/antagonistas & inibidores , Lipopolissacarídeos/farmacologia , Antígeno de Macrófago 1/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Knockout , Regulação para Cima/fisiologiaRESUMO
In the present study, we examine the cell biology of Leishmania amastigote uptake by mammalian cells and compare this process to the phagocytosis of IgG-opsonized erythrocytes. We report that many aspects of amastigote uptake into macrophages resemble classical receptor-mediated phagocytosis. Parasite uptake requires energy expenditure by macrophages but not by parasites. Treating macrophages to prevent either energy metabolism or actin polymerization prevents amastigote uptake. The uptake of amastigotes by macrophages involves the colocalization of f-actin, paxillin, and talin to phagocytic cups that are formed around amastigotes during internalization. Treatment of macrophages with genestein, to inhibit protein phosphorylation, prevents amastigote uptake, indicating that this process, like receptor-mediated phagocytosis, depends on protein tyrosine phosphorylation. However, the amount and the pattern of protein tyrosine phosphorylation observed during amastigote uptake by macrophages is reduced relative to that observed during IgG-erythrocyte phagocytosis. The uptake of viable, but not heat-killed amastigotes, is associated with a decrease in the intensity of several specific macrophage proteins that are phosphorylated on tyrosine residues. In summary, although many features of amastigote uptake by macrophages resemble classical receptor-mediated phagocytosis, differences in macrophage protein phosphorylation during amastigote phagocytosis may contribute to the unique aspects of amastigote uptake and intracellular survival in macrophages.