Association of Polymorphisms in Cytokine genes with susceptibility to Precancerous Lesions and Cervical Cancer: A systematic review with meta-analysis.

Objectives: This study investigated the relationship between single-nucleotide polymorphisms (SNPs) in cytokine genes and the susceptibility to Squamous Intraepithelial Lesions (SIL), cervical cancer and HPV infection through a systematic review with meta-analysis. To verify the effect of SNPs, we also analyzed the transcription factor binding affinity using bioinformatics tools.Methods: Seven electronic databases (MEDLINE, Scielo, BIREME, PubMed, Scopus, Web of Science and Science Direct) were searched for case-control studies.Results: A total of 35 relevant case-control studies were meta-analyzed, including 7 cytokine genes and 15 SNPs. SNPs in IL-17A (rs2275913, rs3748067); IL-17 F (rs763780); IL-12A (rs568408); IL-12B (rs3212227); TNFA (rs1800629, rs361525); IL-1B (rs16944); IL-6 (rs1800795); IL-10 (rs1800896) genes were associated with increased risk for cervical cancer. No association was observed between meta-analyzed polymorphisms and SIL. Additional bioinformatics analysis suggested a possible transcriptional regulation pathway of the TNFA and IL-10 genes through the MZF1 (TNFA -308 G > A and IL-10 - 1082A>G) and ZNF263 (TNFA -238 G > A) transcription factors binding.Conclusion: Overall, 10 SNPs in cytokine genes were associated with increased risk for cervical cancer. Therefore, in our meta-analysis, these SNPs demonstrated to be potential biomarkers for predicting or identifying cases of high risk for SIL and cervical cancer.

Perfil dos diagnósticos de enfermagem de fumicultores / Profile of nursing diagnoses of tobacco growers

Objetivo: descrever os diagnósticos de Enfermagem segundo a taxonomia da American Nursing Diagnosis Association II. Método: trata-se de estudo quantitativo, descritivo, transversal, com 51 produtores de tabaco expostos a agroquímicos que viviam na comunidade rural. Coletaram-se os dados por meio das consultas de Enfermagem. Tabularam-se os dados n Programa Microsoft Excel 2010, posteriormente inseridos no banco de dados utilizando o programa SPSS, versão 22.0, for Windows, e submetidos à revisão e análise estatística. Apresentaram-se os resultados em tabelas. Resultados: verificou-se, por meio das consultas de Enfermagem, que a maioria era do sexo feminino, tinha ensino fundamental incompleto e não praticava nenhum tipo de exercício físico. Acrescenta-se, além disso, que eles desconheciam os riscos para a saúde colocados pelos pesticidas e negligenciaram os padrões de segurança recomendados para o manuseio desses produtos. Conclusão: pode-se observar, a partir deste estudo, que o enfermeiro tem um papel importante no cuidado e na educação, nos diversos espaços de promoção, prevenção e manutenção do cuidado em saúde.(AU)

Objective: to describe Nursing diagnoses according to the Taxonomy II proposed by the North American Nursing Diagnosis Association. Method: this is a quantitative, descriptive, cross-sectional study conducted with co plantations. The research was carried out with 51 tobacco growers exposed to agrochemicals living in the rural community. The data were collected through nursing consultations. The data collected were tabulated in the Microsoft Excel 2010 software and later entered into a database using the SPSS software version 22.0, for Windows, and subjected to statistical review and analysis. The results were presented in tables. Results: the Nursing consultations revealed that the majority of the farm workers were females, had incomplete elementary education, and did not practice any type of physical exercise. In addition, they were unaware of the health risks posed by pesticides and neglected the recommended safety standards for the handling of these products. Conclusion: it was observed in this study that nurses have an important role in care and education, in the various spaces of promotion, prevention and maintenance of health care.(AU)

Objetivo: se buscó describir los diagnósticos de Enfermería según la taxonomía de la American Nursing Diagnosis Association II. Método: se trata de un estudio cuantitativo, descriptivo, transversal, realizado con 51 productores de tabaco expuestos a agroquímicos que vivían en la comunidad rural. Se recogieron los datos por medio de las consultas de Enfermería. Se tabularon los datos recogidos en el programa Microsoft Excel 2010, posteriormente inseridos en el banco de datos utilizando el programa SPSS, versión 22.0, for Windows, y sometidos a la revisión y análisis estadística. Se presentaron los resultados en tablas. Resultados: se verificó, por medio de las consultas de Enfermería, que la mayoría era del sexo femenino, tenía enseñanza primaria incompleta y no praticaba ningún tipo de ejercicio físico. Además de eso, ellos desconocen los riesgos para la salud colocados por los pesticidas y negligenciaron los patrones de seguridad recomendados para el manoseo de esos productos. Conclusión: se puede observar, a partir de este estudio, que el enfermero tiene un papel importante en el cuidado y en la educación, en los diversos espacios de promoción, prevención y mantenimiento del cuidado en salud.(AU)

Association of TNFA (-308G/A), IFNG (+874 A/T) and IL-10 (-819 C/T) polymorphisms with protection and susceptibility to dengue in Brazilian population.

OBJECTIVE: To evaluate gene polymorphisms and their association with susceptibility to dengue. METHODS: A retrospective case-control study was performed with 262 subjects, comprising 78 dengue fever (DF) patients, 49 dengue hemorrhagic fever (DHF) patients and 135 healthy controls. Genotypic and allelic profiles were identified using polymerase chain reaction based in real time and amplification-refractory mutation system. RESULTS: We observed a protective association of IL-10 (-819 C/T) C allele (P = 0.028, OR = 0.56, CI = 0.34-0.91) against DHF, while the C/T (P = 0.047, OR = 2.10, CI = 1.01-4.38) and T/T (P = 0.008, OR = 3.82, CI = 1.38-10.59) genotypes were associated with DHF and DF, respectively. The dominant model TNFA -308 GA + AA (P = 0.043, OR = 0.45, CI = 0.20-1.00) genotypes were found to have protective effect against dengue infection. A protective association among the IFNG (+874 A/T) A/T genotype against DF (P = 0.02, OR = 0.46, CI = 0.24-0.89) and DHF (P = 0.034, OR = 0.43, CI = 0.19-0.95) was observed. When the studied single-nucleotide polymorphism was analyzed in combination, the combination GTA (P = 0.022, OR = 2.95, CI = 1.18-7.41) was statistically significantly associated with susceptibility to DF and the combination GCT (P = 0.035, OR = 0.28, CI = 0.08-0.90) with protection against the development of DHF. CONCLUSIONS: This research identifies the association of the IFNG (+874 A/T), TNFA (-308G/A), IL-10 (-819 C/T) genotypes as a factor for protection, susceptibility and severity to dengue.