RESUMO
The efficacy of a rapid test for detecting PrP(Sc) in central nervous system tissue was evaluated for the postmortem diagnosis of BSE at different times during the course of the disease. One hundred and six samples of brain, at the level of the medulla oblongata, and spinal cord, derived from the experimental study of the pathogenesis of BSE carried out in Great Britain between 1991 and 1995, were examined. PrP(Sc) was detected in the samples from most of the exposed animals killed 32 months or more after they had been exposed to the agent, and before the onset of clinical signs which were first recorded at 35 months. Comparisons with the results of histology, fibril detection, PrP immunohistochemistry and mouse bioassay indicated that the rapid test is at least as sensitive as these conventional confirmatory diagnostic methods and its result can be obtained more quickly.
Assuntos
Sistema Nervoso Central/patologia , Encefalopatia Espongiforme Bovina/diagnóstico , Proteínas PrPSc/análise , Animais , Autopsia/veterinária , Bovinos , Imuno-Histoquímica , Proteínas PrPSc/imunologia , Sensibilidade e EspecificidadeAssuntos
Encefalopatia Espongiforme Bovina/diagnóstico , Ensaio de Imunoadsorção Enzimática/métodos , Proteínas PrPSc/análise , Animais , Bioensaio , Química Encefálica , Bovinos , Surtos de Doenças/veterinária , União Europeia , Programas de Rastreamento/veterinária , Camundongos , Saúde Pública , Kit de Reagentes para Diagnóstico , Sensibilidade e EspecificidadeRESUMO
The situation as regards AD in the European Union in August 1995 is described. The territory has been divided into three zones comprising free regions, regions where eradication programmes are in operation and the remainder. The criteria used for defining these areas are described as are the movement rules applicable to movement into each zone. Considerable activity is now taking place to eradicate this disease and further progress is expected in the near future.