Cardioprotective Effects of the 4-Week Aerobic Running Exercises Before Treatment with Doxorubicin in Rats.

Doxorubicin is associated with cardiotoxicity, and physical exercise seeks to minimize the toxic effects of doxorubicin through physiological cardiac remodeling, as well as the reduction of oxidative stress, evidenced by previous studies. This study aimed to analyze whether running training before treatment with doxorubicin influences tolerance to physical exertion and cardiotoxicity. Thirty-nine male Wistar rats, aged 90 days and weighing between 250 and 300 g, were divided into 4 groups: Control (C), Doxorubicin (D), Trained (T), and Trained + Doxorubicin (TD). Animals in groups T and DT were submitted to treadmill running for 3 weeks, 5 times a week at 18 m/min for 20-30 min before treatment with doxorubicin. Animals in groups D and DT received intraperitoneal injections of doxorubicin hydrochloride three times a week for two weeks, reaching a total cumulative dose of 7.50 mg/kg. Our results show an increase in total collagen fibers in the D group (p = 0.01), but no increase in the TD group, in addition to the attenuation of the number of cardiac mast cells in the animals in the TD group (p = 0.05). The animals in the TD group showed maintenance of tolerance to exertion compared to group D. Therefore, running training attenuated the cardiac damage caused by the treatment with doxorubicin, in addition to maintaining the tolerance to exertion in the rats.

Clinical-pathological alterations, serological and molecular diagnosis in dogs with suspected leptospirosis / Alterações clínico-patológicas, diagnóstico sorológico e molecular em cães com suspeita de leptospirose

Leptospirosis is a globally important zoonosis that causes death in humans and animals, especially unvaccinated dogs. Dogs are infected through contact with urine or water contaminated by pathogenic spirochaete bacteria of the genus Leptospira. This study aimed to assess the performance of two diagnostic tests in characterizing dogs with leptospirosis and associate it with the main clinical-pathological alterations in dogs with suspected infection. From September 2019 to September 2020, 24 dogs with suspected canine leptospirosis were treated at the Veterinary Hospital of the Federal University of Uberlândia (UFU). Complementary tests such as a complete blood count (CBC) and serum creatinine, ALT (alanine transaminase) and ALP (alkaline phosphatase) levels were prescribed. In order to confirm the leptospirosis diagnosis, serum and molecular tests were performed using the microscopic agglutination test (MAT) and real-time quantitative polymerase chain reaction (qPCR). Of the 24 suspected cases of canine leptospirosis, six (25%) were positive in the MAT and three (12.5%) in the qPCR, with one (4.17%) returning positive results in both diagnostic tests. Positive results were obtained for 8/24 (33.3%) for combined MAT + qPCR. The predominant reactive serogroups were Icterohaemorrhagiae (33.33%) and Djasiman (33.33%), followed by Ballum (16.60%). The sensitivity and specificity of the qPCR in relation to the gold standard test (MAT) were 16.7 and 88.9%, respectively. Changes were observed in serum levels of creatinine, urea and ALP in the group that tested positive (MAT/qPCR); however, only urea and ALP levels were high in the negative group. Comparison of biochemical parameters in the positive (MAT/qPCR) and negative groups showed no statistically significant difference between the elements assessed (p>0.05). It was concluded that in order to diagnose acute canine leptospirosis, veterinarians should combine the clinical-pathological findings with paired sample MAT and qPCR urine test results.(AU)

A leptospirose é uma zoonose de importância mundial que causa mortes tanto em humanos, como em animais, especialmente em cães não vacinados. A infecção em cães ocorre pelo contato com urina ou água contaminada pela bactéria espiroqueta patogênica do gênero Leptospira. Objetivou-se avaliar dois testes diagnósticos na caracterização de cães com leptospirose, e associar com as principais alterações clínicos-patológicas de cães suspeitos. Foram atendidos no Hospital Veterinário da Universidade Federal de Uberlândia no período de setembro de 2019 a setembro de 2020, 24 cães com suspeita clínica de leptospirose canina. Exames complementares como hemograma completo, dosagens séricas de creatinina, ureia, ALT (alanina aminotransferase), FA (fosfatase alcalina) foram solicitados. Para o diagnóstico confirmatório de leptospirose foi realizado teste sorológico pelo teste de aglutinação microscópica (MAT) e também teste molecular pela reação em cadeia da polimerase em tempo real (qPCR). Dos 24 casos de cães suspeitos de leptospirose, seis (25%) foram positivos no MAT, e três (12,5%) no qPCR, sendo 1 (um) (4,17%) positivo nos dois testes diagnósticos avaliados. Quando se associou MAT + qPCR a positividade foi para 8/24 (33,3%). Os sorogrupos reativos predominantes foram Icterohaemorrhagiae (33,33%) e Djasiman (33,33%), seguido de Ballum (16,60%). A sensibilidade e especificidade da qPCR em relação teste padrão ouro (MAT) foi 16,7 % e 88,9%, respectivamente. Houve alterações nos níveis séricos de creatinina, ureia e FA no grupo positivo aos testes diagnósticos (MAT/qPCR), porém somente ureia e FA apresentaram valores elevados no grupo negativo. Quando se comparou os parâmetros bioquímicos no grupo positivo aos testes diagnósticos (MAT/qPCR), e negativo, não se observou nenhuma diferença estatística significativa entre os elementos avaliados (p>0,05). Conclui-se para o diagnóstico de leptospirose canina aguda, o médico veterinário deve associar os achados clínicos-patológicos aos resultados do MAT de forma pareada e o qPCR de urina.(AU)

Physiological Changes in Chicken Embryos Inoculated with Drugs and Viruses Highlight the Need for More Standardization of this Animal Model.

Several studies have been developed using the Gallus gallus embryo as an experimental model to study the toxicity of drugs and infections. Studies that seek to standardize the evaluated parameters are needed to better understand and identify the viability of CEs as an experimental model. Therefore, we sought to verify whether macroscopic, histopathological, blood count, metabolites and/or enzymes changes and oxidative stress in CE of different ages are specific to the model. To achieve this goal, in ovo assays were performed by injecting a virus (Gammacoronavirus) and two drugs (filgrastim and dexamethasone) that cause known changes in adult animals. Although congestion and inflammatory infiltrate were visible in the case of viral infections, the white blood cell count and inflammation biomarkers did not change. Filgrastim (FG) testing did not increase granulocytes as we expected. On the other hand, CE weight and red blood cell count were lower with dexamethasone (DX), whereas white blood cell count and biomarkers varied depended on the stage of CE development. Our work reinforces the importance of standardization and correct use of the model so that the results of infection, toxicity and pharmacokinetics are reproducible.

Influence of management and lactation order in the serum concentrations of proteins, metabolites, minerals, and enzymes of Bonsmara breed cows / Influência da gestação e ordem de lactação nas concentrações séricas de proteínas, metabólitos, minerais e enzimas de vacas da raça Bonsmara

This study aimed to evaluate the influence of gestation and lactation order on serum concentrations of proteins, metabolites, minerals, and enzymes in first-, second-, and third-lactation order Bonsmara cows. The Bonsmara breed, originated in South Africa, stands out for its tolerance to heat and productive characteristics, such as meat quality, high fertility, sexual precocity, ease of calving, and good maternal ability. These characteristics make the breed a genetic alternative for crossbreeding with zebu breeds. Blood samples were collected from 34, 29, and 30 first-, second-, and third-lactation order cows, respectively, totaling 93 cows. The samples were processed in an automatic multichannel analyzer using Labtest Diagnóstica® kits. The Kruskal-Wallis test was chosen to compare the values between lactation orders. The Mann-Whitney test was used to compare pregnant and non-pregnant cows within lactation orders. Among the analyzed constituents, the lactation order significantly influenced only the serum albumin concentration (ALB) (p<0.0001). Gestation significantly influenced the A:G ratio (p=0.034) in third-lactation cows, and cholesterol (Chol) (p=0.004), triglycerides (TRI) (p<0.0001), inorganic phosphorus (iP) (p=0.033), iron (Fe) (p=0.001), aspartate aminotransferase (AST) (p=0.018), and alkaline phosphatase (ALP) (p=0.039) in second-lactation cows and the general group. Creatinine (Crea) (p<0.0001) was influenced only in the general group. Therefore, gestation and lactation order significantly influenced the concentration of several biochemical serum constituents in Bonsmara cows, especially second-lactation order cows.

Objetivou-se avaliar a influência da gestação e ordem de lactação nas concentrações séricas de proteínas, metabólitos, minerais e enzimas de vacas da raça Bonsmara de primeira, segunda e terceira ordem de lactação. A raça Bonsmara, originada na África do Sul, se destaca por sua tolerância ao calor, e caracteristicas produtivas, como a qualidade da carne, a alta fertilidade, precocidade sexual, facilidade ao parto e boa habilidade materna. Essas características tornam a raça uma alternativa genética para realização de cruzamentos com raças zebuínas. Foram colhidas amostras de sangue de 93 vacas, sendo 34 de primeira ordem de lactação, 29 de segunda ordem de lactação e 30 de terceira ordem de lactação. As amostras foram processadas em analisador automático multicanal, utilizando kits da Labtest Diagnóstica®. Para confrontar os valores entre as ordens de lactação optou-se pelo teste Kruskall-Wallis. Para comparar vacas gestantes e não gestantes dentro das ordens de lactação, foi utilizado o teste de Mann-Whitney. Dos constituintes analisados, a ordem de lactação influenciou significativamente apenas a concentração sérica de albumina (ALB) (p<0,0001). A gestação influenciou significativamente na relação A:G (p=0,034), nas vacas de terceira lactação, no colesterol (COL) (p=0,004), triglicérides (TRI) (p<0,0001), fósforo inorgânico (Pi) (p=0,033), ferro (Fe) (p=0,001), aspartato aminotransferase (AST) (p=0,018) e fosfatase alcalina (FAL) (p=0,039) nas de segunda ordem e no valor do grupo geral. A creatinina (Crea) (p<0,0001) foi influenciada somente no grupo geral. Conclui-se que a gestação e ordem de lactação influenciou significativamente na concentração de vários constituintes bioquímicos séricos de vacas da raça Bonsmara, em especial nas de segunda ordem de lactação.

Influence of age and sex on blood biochemical profile of Bonsmara cattle breed up to two years / Influência da idade e sexo no perfil bioquímico sanguíneo de bovinos da raça Bonsmara até dois anos

This study aimed to evaluate the effects of animal age and sex on serum concentrations of proteins, metabolites, minerals, and enzymes in Bonsmara cattle up to two years of age. Blood samples were collected from 179 animals (92 males and 87 females), aged between 15 days and 24 months. The animals were divided into four age groups: G1: 15 days ┤2 months, G2: 2 ┤6 months, G3: 6 ┤ 12 months, and G4: 12 ┤24 months of age. The samples were processed in an automatic multichannel analyzer using Labtest Diagnóstica™ kits. Animal age had a significant influence on most of the serum biochemical constituents except for magnesium (Mg) and the Ca+:iP ratio. As age increased, G3 showed the highest concentrations of total proteins (TP), globulins (Glob), urea, and cholesterol (Chol); while G4 had a gradual increase and higher values of creatinine (Crea), aspartate aminotransferase (AST), and alanine aminotransferase (ALT). Conversely, albumin:globulin (A:G) ratio, calcium (Ca+), inorganic phosphorus (iP), alkaline phosphatase (ALP), and gamma-glutamyltransferase (GGT) decreased from G1 to G4. Moreover, animal sex influenced serum values of albumin (ALB), Glob, A:G ratio, Crea, urea, triglycerides (TRI), Ca+, iP, Ca+:iP ratio and Mg. In conclusion, the factors of age group and sex significantly influenced the concentrations of serum biochemical constituents in Bonsmara cattle in the growth phase.

Objetivou-se avaliar a infuência da idade e sexo nas concentrações séricas de proteínas, metabólitos, minerais e enzimas em bovinos da raça Bonsmara com até dois anos de idade. Foram colhidas amostras de sangue de 179 animais (92 machos e 87 fêmeas), de 15 dias a 24 meses de idade, distribuídos em quatro grupos etários: G1: 15 dias ┤2 meses, G2: 2 ┤6 meses, G3: 6 ┤12 meses e G4: 12 ┤24 meses de idade. As amostras foram processadas em analisador automático multicanal, utilizando kits da Labtest Diagnóstica®. A variável idade dos animais influenciou significativamente nos valores da maioria dos constituintes bioquímicos séricos avaliados, exceto no magnésio (Mg) e na relação Ca+:Pi. O aumento da idade culminou com a maior concentração das proteínas totais (PT), globulinas (Glob), ureia e colesterol (COL) no G3; aumento gradual e maior valor de creatinina (Crea), aspartato aminotransferase (AST) e alanina aminotransferase (ALT) no G4. Em contrapartida, houve diminuição da relação albumina:globulina (A:G), cálcio (Ca+), fósforo inorgânico (Pi), fosfatase alcalina (FAL) e gama glutamiltransferase (GGT) do G1 ao G4. O fator sexo influenciou nos valores séricos da albumina (ALB), Glob, relação A:G, Crea, ureia, triglicérides (TRI), Ca+, Pi, relação Ca+:Pi e Mg. Conclui-se que o fator idade e sexo influenciaram significativamente nos valores dos constituintes bioquímicos séricos analisados de bovinos da raça Bonsmara em fase de crescimento.

Influence of age and sex on the blood biochemical constituent values of broiler breeders during the egg-laying stage.

This study aimed to evaluate the influence of age and sex on the blood biochemical constituents of broiler breeders during the egg production stage. The analysis was performed in an industrial broiler breeder farm, and blood samples were collected from males and females at five different ages. Biochemical elements analysed in each serum sample were total proteins, albumin, globulins, uric acid, total cholesterol and triglycerides, alanine aminotransferase (ALT) and aspartate aminotransferase (AST), gamma-glutamyltransferase (GGT), creatine kinase (CK), alkaline phosphatase (PAL), calcium and phosphorus, beside the glycaemic status. At most ages, females had higher values of total proteins, albumin, globulins, triglycerides, cholesterol, glucose, calcium, phosphorus, Ca/P (calcium and phosphorus) ratio and gamma-glutamyltransferase (GGT). The values of uric acid, aspartate aminotransferase (AST), creatine kinase (CK) and alkaline phosphatase (PAL) were higher in males. The lowest protein values were found at 28 and 60 weeks old. The mean albumin values were significantly higher at 44 and 52 weeks old in males and females. A trend of increasing globulin values with increasing age up to 52 weeks old was observed. Although calcium and phosphorus did not vary according to age, the Ca/P ratio was lowest at 36 weeks old. Comparing the means of both sexes, the AST and GGT values were significantly higher in 60-week-old birds. The highest serum levels of CK occurred at 28 and 52 weeks old. The physiological levels of serum biomarkers presented in this work are important for evaluating productivity performance, welfare and disease indication in breeding flocks.

Biochemical serum profile of quarter mile equines in team penning training / Caracterização do perfil bioquímico sérico em equinos quarto de milha submetidos ao treinamento para team penning

The objective of this work was to evaluate the proteins, minerals, metabolites, and serum enzymes profile in Quarter Mile equines subjected to training for Team Penning competitions by comparing the results before and after training. The animals were from the Agricultural Exhibition Park of Uberlândia (CAMARU), in Uberlândia in the State of Minas Gerais, Brazil. These animals perform constant periodic training with an average duration of two and a half hours per training. Blood samples from twenty male and female equines were collected at the beginning and end of a 45-day interval and then analyzed. A 5 mL blood sample from each animal was collected before and after training by a jugular external venipuncture, and the samples were placed in a tube­without anticoagulant and containing separator gel­to obtain the serum. The components analyzed were: total proteins, albumin, globulins, albumin to globulin ratio (A:G), total and ionized calcium, phosphorus, calcium to phosphorus ratio (Ca:P), cholesterol, triglycerides, creatinine, urea, alkaline phosphatase, ɤ-glutamyl transferase, lactate dehydrogenase (LDH), aspartate amino transferase (AST), alanine aminotransferase (ALT) and creatine kinase (CK). Statistical differences were observed with higher values after exercise for total calcium, ionized calcium, Ca: P ratio, creatinine and ALT and decreased concentrations were found for globulins, phosphorus, cholesterol and triglycerides. Despite the changes observed in the serum concentrations of some elements after exercises, it was concluded that the animals were in adequate physical condition to perform the proposed physical exercises.

O presente estudo objetivou comparar o perfil de proteínas, metabólitos, minerais e enzimas séricas em equinos Quarto de Milha submetidos a treinamento para provas de Team Penning no Parque de Exposições Agropecuárias de Uberlândia (CAMARU), Uberlândia-MG. Os animais realizavam treinos periódicos e constantes com duração de duas horas e meia em média. Foram analisadas 40 (quarenta) amostras de sangue de 20 (vinte) equinos, machos e fêmeas, coletadas em dois momentos com intervalo de 45 dias. Por meio de venopunção jugular externa, coletou-se 05 (mL) de sangue em tubo sem anticoagulante e contendo gel separador para obtenção de soro, antes e após o treinamento de cada momento. Os constituintes analisados foram proteínas totais, albumina, globulinas, relação albumina globulina (A:G), cálcio total e ionizado, fósforo, relação cálcio fósforo (Ca:P), colesterol, triglicérides, creatinina, ureia, fosfatase alcalina, ɤ-glutamiltransferase (GGT), lactato desidrogenase (LDH), aspartato aminotransferase (AST), alanina aminotransferase (ALT) e creatina quinase (CK). Sendo observado diferenças estatísticas com valores mais elevados após o exercicio para cálcio total, cálcio ionizado, relação Ca:P, creatinina e ALT e concentrações diminuidas para globulinas, fósforo, colesterol e triglicérides. Apesar das alterações observadas nas concentrações séricas de alguns elementos após a prática esportiva, conclui-se que os animais se encontravam em condicionamento físico adequado para realizarem os exercícios físicos propostos.

Efeito das estações do ano e da idade sobre as variáveis termofisiológicas e hematológicas de bezerros leiteiros mestiços em ambiente tropical / Effect of season and age on thermophysiological and hematological variables of crossbred dairy calves in tropical environment

Background: The wellbeing and performance of calves may be impaired if raised in high temperature environments. Physiological and hematological variables serve as a tool to assess the welfare of cattle; therefore, the influence of seasons and age on physiological and hematological variables was evaluated of crossbred dairy calves in a tropical environment.Materials, Methods & Results: Forty-two male and female calves were evaluated from July 2016 to July 2017, when the calves were of 2, 15, 30, and 60 days of age. Respiratory (RR) and heart rates (HR) and rectal (RT) and body surface (BST) temperatures were quantified in the morning. Additionally, in the morning, dry bulb (Tdb) and wet bulb (Twb) temperatures were measured, and then, relative humidity (RH) and a temperature-humidity index (THI) were calculated. Blood was collected to perform hemogram. The day before the calves were evaluated, thermal environment data were collected to verify whether there were cyclic periods of heat stress. The mean air temperature ranged from 19°C to 22.1°C, and the highest THI value of 68 occurred in the summer mornings. In the afternoon, the maximum air temperature ranged from 27.5°C to 29.7°C. In autumn, respiratory and heart rates and body surface temperature were higher in 2-day-old calves than in other ages. The body surface temperature of 60-day-old calves was higher in spring and summer than in autumn. Rectal temperature remained in the normal range throughout the study period. Season did not influence the erythrogram and plaquetogram. The values for red blood cells, MCV, MCHC, RDW, platelets, and MPV varied among the calf ages. Season did not influence the values of leukocytes, monocytes, lymphocytes, or N/L ratio; however, band neutrophils and eosinophils varied among seasons. Band neutrophils and monocytes were not altered by age, whereas leukocyte, segmented neutrophils, eosinophils, lymphocytes, and N/L ratio values varied with age among the calves.[...]

A retrospective study of reproductive disorders in female dogs from the city of Uberlândia, Minas Gerais, Brazil / Estudo retrospectivo de desordens reprodutivas em cadelas no município de Uberlândia, Minas Gerais, Brasil

Diseases of the reproductive system are common in both sexes in the most varied of species. In female dog, these diseases produce different degrees of morbidity and mortality and are influenced by environmental conditions, reproductive history, and previous drug treatments. Reproductive disease may have different consequences, ranging from the absence of clinical signs to the impairment of fertility. The objective of this study was to determine the causes of reproductive diseases in female dogs admitted to the Veterinary Hospital, Federal University of Uberlândia, during a six-year period (2012-2017) and to identify the most frequently occurring diseases. Information relative to the species, age, breed, and sex of 32,944 were obtained for animals. Of these, 16,480 were female dogs and 1,185 were diagnosed with alterations to the reproductive system. The animals were divided into four groups according to age (puppies, young adults, adults, and old) and disease of the reproductive system (Group 1 - vaginal and vulvar alterations; Group 2 - alterations to the estrous cycle, ovary, and uterus; Group 3 - alterations to the gestational period and parturition). The highest occurrence of diagnoses (47%) was recorded in young adult female dogs (1 to 5 years old). The most frequent alterations in female dogs were pyometra (48.8%), dystocia (13.6%), and transmissible venereal tumor (TVT) (12.6%). Crossbred dogs were the most affected with alterations to the reproductive system, representing 60% of all the female dogs diagnosed with any pathologic alteration. In conclusion, a 7.2% occurrence of reproductive disorders shows the relevance of these diseases in small animals and suggests that new methods are needed for the prevention, early diagnosis, and treatment of these disorders.

Enfermidades do sistema reprodutor são comuns, para ambos os sexos, nas mais variadas espécies. Em cadelas estas doenças têm diferentes graus de morbidade e mortalidade e são influenciadas por condições ambientais, histórico reprodutivo e tratamentos prévios com fármacos. As afecções reprodutivas podem gerar consequências distintas, variando desde a ausência de sinais clínicos até o comprometimento da fertilidade do animal. Objetivou-se determinar a casuística das enfermidades reprodutivas de cadelas atendidas no Hospital Veterinário da Universidade Federal de Uberlândia, durante seis anos de avaliação (2012-2017), a fim de identificar quais enfermidades eram mais frequentes. Para tanto, foram consultadas informações sobre espécie, idade, raça e sexo de 32.944 animais. Destes, 16.480 eram fêmeas caninas e 1.185 foram diagnosticadas com alterações no sistema reprodutivo. Os animais foram divididos de acordo com a idade em quatro grupos (filhotes, adultos jovens, adultos e idosos); e com as enfermidades do sistema reprodutivo (Grupo 1 - alterações de vagina e vulva; Grupo 2 - alterações de ciclo estral, ovário e útero; Grupo 3 - alterações do período gestacional e parto). As médias de idades que apresentaram maior ocorrência de diagnósticos foram em cadelas adultas jovens (1 a 5 anos) com 47%. As patologias mais frequentes em fêmeas caninas foram a piometra (48,8%), distocias (13,6%) e Tumor Venéreo Transmissível (TVT) (12,6%). Os animais mestiços (SRD) foram os mais acometidos por distúrbios do sistema reprodutor, representando 60% do total de fêmeas diagnosticadas. Concluiu-se que a ocorrência de 7,2% de desordens reprodutivas mostra a relevância dessas doenças na clínica de pequenos animais e contribui para o reconhecimento de que novos métodos de prevenção, diagnóstico precoce e tratamento dessas desordens são necessários.

A retrospective study of reproductive disorders in female dogs from the city of Uberlândia, Minas Gerais, Brazil / Estudo retrospectivo de desordens reprodutivas em cadelas no município de Uberlândia, Minas Gerais, Brasil

Diseases of the reproductive system are common in both sexes in the most varied of species. In female dog, these diseases produce different degrees of morbidity and mortality and are influenced by environmental conditions, reproductive history, and previous drug treatments. Reproductive disease may have different consequences, ranging from the absence of clinical signs to the impairment of fertility. The objective of this study was to determine the causes of reproductive diseases in female dogs admitted to the Veterinary Hospital, Federal University of Uberlândia, during a six-year period (2012-2017) and to identify the most frequently occurring diseases. Information relative to the species, age, breed, and sex of 32,944 were obtained for animals. Of these, 16,480 were female dogs and 1,185 were diagnosed with alterations to the reproductive system. The animals were divided into four groups according to age (puppies, young adults, adults, and old) and disease of the reproductive system (Group 1 - vaginal and vulvar alterations; Group 2 - alterations to the estrous cycle, ovary, and uterus; Group 3 - alterations to the gestational period and parturition). The highest occurrence of diagnoses (47%) was recorded in young adult female dogs (1 to 5 years old). The most frequent alterations in female dogs were pyometra (48.8%), dystocia (13.6%), and transmissible venereal tumor (TVT) (12.6%). Crossbred dogs were the most affected with alterations to the reproductive system, representing 60% of all the female dogs diagnosed with any pathologic alteration. In conclusion, a 7.2% occurrence of reproductive disorders shows the relevance of these diseases in small animals and suggests that new methods are needed for the prevention, early diagnosis, and treatment of these disorders.(AU)

Enfermidades do sistema reprodutor são comuns, para ambos os sexos, nas mais variadas espécies. Em cadelas estas doenças têm diferentes graus de morbidade e mortalidade e são influenciadas por condições ambientais, histórico reprodutivo e tratamentos prévios com fármacos. As afecções reprodutivas podem gerar consequências distintas, variando desde a ausência de sinais clínicos até o comprometimento da fertilidade do animal. Objetivou-se determinar a casuística das enfermidades reprodutivas de cadelas atendidas no Hospital Veterinário da Universidade Federal de Uberlândia, durante seis anos de avaliação (2012-2017), a fim de identificar quais enfermidades eram mais frequentes. Para tanto, foram consultadas informações sobre espécie, idade, raça e sexo de 32.944 animais. Destes, 16.480 eram fêmeas caninas e 1.185 foram diagnosticadas com alterações no sistema reprodutivo. Os animais foram divididos de acordo com a idade em quatro grupos (filhotes, adultos jovens, adultos e idosos); e com as enfermidades do sistema reprodutivo (Grupo 1 - alterações de vagina e vulva; Grupo 2 - alterações de ciclo estral, ovário e útero; Grupo 3 - alterações do período gestacional e parto). As médias de idades que apresentaram maior ocorrência de diagnósticos foram em cadelas adultas jovens (1 a 5 anos) com 47%. As patologias mais frequentes em fêmeas caninas foram a piometra (48,8%), distocias (13,6%) e Tumor Venéreo Transmissível (TVT) (12,6%). Os animais mestiços (SRD) foram os mais acometidos por distúrbios do sistema reprodutor, representando 60% do total de fêmeas diagnosticadas. Concluiu-se que a ocorrência de 7,2% de desordens reprodutivas mostra a relevância dessas doenças na clínica de pequenos animais e contribui para o reconhecimento de que novos métodos de prevenção, diagnóstico precoce e tratamento dessas desordens são necessários.(AU)

Efeito das estações do ano e da idade sobre as variáveis termofisiológicas e hematológicas de bezerros leiteiros mestiços em ambiente tropical / Effect of season and age on thermophysiological and hematological variables of crossbred dairy calves in tropical environment

Background: The wellbeing and performance of calves may be impaired if raised in high temperature environments. Physiological and hematological variables serve as a tool to assess the welfare of cattle; therefore, the influence of seasons and age on physiological and hematological variables was evaluated of crossbred dairy calves in a tropical environment.Materials, Methods & Results: Forty-two male and female calves were evaluated from July 2016 to July 2017, when the calves were of 2, 15, 30, and 60 days of age. Respiratory (RR) and heart rates (HR) and rectal (RT) and body surface (BST) temperatures were quantified in the morning. Additionally, in the morning, dry bulb (Tdb) and wet bulb (Twb) temperatures were measured, and then, relative humidity (RH) and a temperature-humidity index (THI) were calculated. Blood was collected to perform hemogram. The day before the calves were evaluated, thermal environment data were collected to verify whether there were cyclic periods of heat stress. The mean air temperature ranged from 19°C to 22.1°C, and the highest THI value of 68 occurred in the summer mornings. In the afternoon, the maximum air temperature ranged from 27.5°C to 29.7°C. In autumn, respiratory and heart rates and body surface temperature were higher in 2-day-old calves than in other ages. The body surface temperature of 60-day-old calves was higher in spring and summer than in autumn. Rectal temperature remained in the normal range throughout the study period. Season did not influence the erythrogram and plaquetogram. The values for red blood cells, MCV, MCHC, RDW, platelets, and MPV varied among the calf ages. Season did not influence the values of leukocytes, monocytes, lymphocytes, or N/L ratio; however, band neutrophils and eosinophils varied among seasons. Band neutrophils and monocytes were not altered by age, whereas leukocyte, segmented neutrophils, eosinophils, lymphocytes, and N/L ratio values varied with age among the calves.[...](AU)

Systemic alterations induced by phospholipase A2 , BmooTX-I, isolated from Bothrops moojeni snake venom.

Ophidic accidents are among the problems of public health in Brazil. The components from bothropic venom are responsible for many systemic clinical complications resulting from envenomation. The present work aimed to analyse the systemic changes induced in mice after intraperitoneal administration of BmooTX-I, a myotoxic acidic phospholipase A2 isolated from Bothrops moojeni venom. Urinalysis was performed and the following plasma biochemical markers were documented: urea, creatinine and uric acid (renal function); glucose and amylase (pancreatic function); alanine aminotransferase, alkaline phosphatase and gamma-GT (intra- and extrahepatic function); creatine kinase and enzymatic lactate (muscle function). Our results showed that after the intraperitoneal injection of BmooTX-I the urine of these animals showed glycosuria, proteinuria, haematuria, bacteriuria, bilirubinuria, polyuria and nitrite. The plasma biochemical analysis showed alterations in levels of urea, creatinine and uric acid. Amylase concentration was not altered significantly, but the plasma glucose increased significantly compared to controls. The plasma levels of alanine aminotransferase and alkaline phosphatase decreased and increased, respectively, in these same animals. On the other hand, the plasma γGT concentration did not undergo significant modification compared to the control group. The plasma concentration of CK increased, while the enzymatic lactate concentration decreased after the injection of the BmooTX-I. Therefore, in mice BmooTX-I is capable of causing systemic alterations which manifest as renal, muscular, hepatic and pancreatic impairment.

Physiological variations of serum biochemical profile in quarter horse mares at peripartum and in their neonatal foals / Variações fisiológicas no perfil bioquímico sérico de éguas quarto de milha no periparto e potros neonatos

The objectives of this study were to evaluate the serum biochemical profile in Quarter Horse mares in their peripartum, and neonates on their birth date, and to compare the values obtained for male and female foals, as well as values obtained for foals and mares on the day of delivery. Forty one adult, pregnant mares and their respective offspring on the day of delivery were analyzed, totaling 82 animals. Two samples of blood were collected from the mares, seven days before and on the day of delivery. From the foals, blood collection was performed on the day of their birth about 12 h after colostrum ingestion. The samples were centrifuged and the serum stored at -20 ºC for analyses. Each sample was used to determine the serum concentrations proteins, metabolites, minerals and enzymes. Physiological changes caused by peripartum led to higher serum total protein, albumin, and globulins on the day of delivery. Serum concentration of triglycerides and calcium /phosphorus ratio were greater seven days before delivery; iron concentration was higher than reference ranges in both moments of evaluation. In foals, albumin serum concentrations, albumin/globulins ratio, and aspartate aminotransferase remained below reference ranges. In foals, there was no influence of sex in the serum biochemical profile. When results obtained for foals on their birth date were compared with those obtained for the mares on the delivery day, cholesterol, triglycerides, phosphorus, iron, alkaline phosphatase, and gamma glutamyltransferase were higher in foals, and total protein, albumin, globulins, urea nitrogen, calcium/phosphorus ratio, and aspartate aminotransferase were higher in mares. Results showed variations in serum biochemistry in peripartum mares and neonates, as well as in the comparison between foals and mares on the day of delivery. Clinically, serum biochemistry values for peripartum mares and neonates should be considered in the analysis of laboratory results of tests carried out in this period.

Os objetivos deste estudo foram avaliar o perfil bioquímico sérico de éguas no periparto e potros no dia do nascimento, e confrontar os valores obtidos para potros machos e fêmeas, e desses com as éguas no dia do parto. Foram utilizadas 41 éguas adultas, gestantes, e suas respectivas crias no dia do nascimento, totalizando 82 animais. Das éguas foram coletadas duas amostras de sangue, sete dias antes do parto e no dia do parto. Dos potros, no dia do nascimento, aproximadamente 12 h após ingestão do colostro. As amostras foram centrifugadas e o soro estocado à ­ 20º C para análises. De cada amostra foram determinadas as concentrações séricas de proteínas, metabólitos, minerais e enzimas. Alterações fisiológicas decorrentes do periparto determinaram um quadro com maiores valores sérico das proteínas totais, albumina e globulinas no dia do parto. A concentração sérica dos triglicérides e a relação cálcio/fósforo foi maior aos sete dias antes do parto e, o ferro com valores superiores aos de referência, nos dois momentos avaliados. Nos potros as concentrações séricas da albumina, relação albumina/globulinas e aspartato aminotransferase permaneceram abaixo dos valores de referência. Não houve influencia do sexo nos valores dos constituintes bioquímicos séricos avaliados. Quando confrontados os resultados obtidos para potros, no dia do nascimento, com os das éguas no dia do parto, observou-se maiores valores do colesterol, triglicérides, fósforo, ferro, fosfatase alcalina e gama glutamiltransferase nos potros, e nas éguas maiores valores para proteínas totais, albumina, globulinas, nitrogênio ureico, relação cálcio/fósforo e aspartato aminotransferase. Os resultados revelaram variações nos valores dos constituintes bioquímicos séricos nas éguas no peripato, nos potros neonatos e dos potros em relação as éguas no dia do parto. Clinicamente, os valores dos constituintes bioquímicos séricos nas éguas no periparto e potros neonatos, devem ser considerados no momento da interpretação de resultados de análises laboratoriais nesses períodos.

Metabolites able to predict uterine diseases in crossbred dairy cows during the transition period / Metabólitos capazes de predizer enfermidades uterinas em vacas leiteiras mestiças no período de transição

This study aimed to identify possible metabolites able to predict puerperal uterine diseases in crossbred dairy cows (Holstein x Gir) in the transition period. We hypothesized that, as what has been observed for Holstein cows, a negative energy balance (NEB) may increase the incidence of uterine diseases. Thirty-four lactating crossbred cows (½HG, »HG, ¾HG, and 5/8HG) were evaluated during prepartum (-60d and -30d), calving, and postpartum (+14d, +21d, and +35d) periods, being divided into two groups (with and without uterine diseases). NEB evaluations were based on the concentrations of non-esterified fatty acids (NEFA) and beta-hydroxybutyrate (BHBA) at calving and 14 days postpartum. Nearly 1/3 of the crossbred cows presented an NEB, characterized by a significant increase in NEFA with no BHBA increase; however, it was less intense than that observed in Holstein cows during the transition period. There was a significant difference between cows with and without uterine diseases in NEFA and calcium concentrations at partum, as well as body condition score (BCS) during prepartum. In conclusion, the occurrence of uterine diseases during the transitional period is related to the BCS in the prepartum period, besides serum NEFA and calcium concentrations at partum.(AU)

O objetivo do estudo foi identificar possíveis metabólitos no período de transição capazes de predizer a ocorrência de enfermidades uterinas puerperais em vacas leiteiras (Holandês X Gir). A hipótese foi de que, semelhante ao observado em vacas Holandesas, o balanço energético negativo (BEN) aumentaria a incidência de enfermidades uterinas. Foram utilizadas 34 vacas lactantes com variação entre ½HG, »HG, ¾HG e 5/8HG de composição genética. Os momentos avaliados foram estabelecidos como pré-parto (-60d e -30d), parto e pós-parto (+14d, +21d e +35d) e as fêmeas divididas em dois grupos, com e sem enfermidades uterinas. O BEN foi estabelecido com base nas concentrações de ácidos graxos não-esterificados (NEFA) e beta-hidroxibutirato (BHBA) no parto e 14 dias pós-parto. Observou-se que cerca de 1/3 das vacas mestiças passaram por BEN caracterizado por aumento significativo de NEFA e sem aumento de BHBA, porém em intensidade menor do que o observado em vacas Holandesas. Verificou-se diferença significativa do escore de condição corporal (ECC) no pré-parto e das concentrações de NEFA e de cálcio no parto, entre vacas com e sem enfermidades uterinas. Conclui-se que o ECC no pré-parto e as concentrações de NEFA e cálcio no parto se relacionam com a ocorrência de enfermidades uterinas.(AU)

Perfil bioquímico sérico de tilápias do Nilo (Oreochromis niloticus) criadas em tanques rede durante as estações do verão e inverno / Serum biochemical profile of Nile tilapias (Oreochromis niloticus) bred in net cages during summer and winter

Background: The growth of fish farming in Brazil has been noticeable over the past few years, and the Nile tilapia (Oreochromis niloticus) is the most bred species in the country. Studies on the physiology of these animals are necessary so that the production continues to grow and to improve its rates. However, very discrepant data are still found in the area of blood biochemistry. The aim of this study was to evaluate the physiological variations in serum concentrations of proteins, metabolites, electrolytes and serum enzymes in Nile tilapias bred in net cages in Cachoeira Dourada, state of Minas Gerais, Brazil, during summer and winter. Materials, Methods & Results: During the year of 2016, samples from 100 Nile tilapias were collected during summer (January and February) and samples from 100 Nile tilapias were also collected during winter (July and August). Only one blood sample was collected by caudal venipuncture of each fish, just before the slaughter, always in the morning. The serum samples obtained were then analyzed for serum concentrations of proteins, metabolites, electrolytes, lipids and serum enzymes. Serum concentrations of total proteins, globulins, triglycerides, high density lipoprotein (HDL), alkaline phosphatase, sodium and chloride were significantly higher during winter in comparison to summer, while the serum concentration of uric acid was significantly lower during winter in comparison to summer. Discussion: Due to the lower temperature of water during winter, tilapias reduced food intake and became more susceptible to diseases, which led to an increase in circulating globulins. In that same season, there was an increase in serum concentrations of sodium and chloride as a result of adding sodium chloride to the water in order to stimulate mucus secretion on the fishes’ skin and gills to prevent pathologies. Triglycerides, HDL, and uric acid are considered indicators of nutritional status of animals.[...]

Eficácia anti-helmíntica da ivermectina em equinos: exames coproparasitológicos e hematológicos / Antihelminthic efficacy of ivermectin in horses: coproparasitological and hematological exams

O objetivo do presente estudo foi avaliar a eficácia da ivermectina a 2% em equinos, através de exames coproparasitológicos e hematológicos. O experimento foi realizado em uma fazenda no município de Uberlândia, MG. Foram utilizados 24 equinos, de três a oito anos, divididos em dois grupos, conforme valores dos OPG. Contagens de OPG e coproculturas foram realizadas nos dias D0, D+7 e D+14. Os animais foram tratados com a formulação pasta a base de ivermectina 2%, na dose de 200 mcg/kg, via oral. Amostras de sangue foram coletadas nos dias D0, D+7 e D+14 para avaliação dos parâmetros hematológicos. A presença de ovos, característicos da família Strongylidae e identificação de larvas características de ciatostomíneos demonstrou o parasitismo dos animais por pequenos estrôngilos. Nos D+7 e D+14 houve a redução significativa do OPG no grupo tratado com eficácia de 99,5 e 99,7%, respectivamente. Observou-se tendência do quadro eritrocitário manter-se próximo aos limites inferiores e discreta leucocitose com neutrófilos próximos dos valores superiores. Pode-se inferir que a ivermectina é eficaz contra os ciatostomíneos e alterações hematológicas significativas não foram observadas.

The aim of the present study was to evaluate the efficacy of ivermectin at 2% in horses, through coproparasitological and hematological exams. The experiment was performed at a farm in the city of Uberlândia, Minas Gerais State. Twenty-four horses were used, ranging from 3 to 8 years of age, divided into 2 groups according to EPG values. EPG counts and coprocultures were performed on days D0, D+7 and D+14. The animals were treated with the ivermectin 2%-based paste formulation at a dose of 200 mcg/kg, orally. Blood samples were collected on days D0, D+7 and D+14 for evaluation of haematological parameters. The presence of eggs that are characteristic of the Strongylidae family and the identification of larvae that are characteristic of cyathostomins demonstrated the parasitism of the animals by small strongyles. At D+7 and D+14 there was a significant reduction of EPG in the treated group with an efficacy of 99.5 and 99.7%, respectively. A tendency of the erythrocyte framework to remain close to the lower limits was observed, besides a slight leukocytosis, with neutrophils close to the highest values. It can be inferred that ivermectin is effective against cyastostomines, and no significant haematological changes were observed.

Metabolites able to predict uterine diseases in crossbred dairy cows during the transition period / Metabólitos capazes de predizer enfermidades uterinas em vacas leiteiras mestiças no período de transição

This study aimed to identify possible metabolites able to predict puerperal uterine diseases in crossbred dairy cows (Holstein x Gir) in the transition period. We hypothesized that, as what has been observed for Holstein cows, a negative energy balance (NEB) may increase the incidence of uterine diseases. Thirty-four lactating crossbred cows (½HG, »HG, ¾HG, and 5/8HG) were evaluated during prepartum (-60d and -30d), calving, and postpartum (+14d, +21d, and +35d) periods, being divided into two groups (with and without uterine diseases). NEB evaluations were based on the concentrations of non-esterified fatty acids (NEFA) and beta-hydroxybutyrate (BHBA) at calving and 14 days postpartum. Nearly 1/3 of the crossbred cows presented an NEB, characterized by a significant increase in NEFA with no BHBA increase; however, it was less intense than that observed in Holstein cows during the transition period. There was a significant difference between cows with and without uterine diseases in NEFA and calcium concentrations at partum, as well as body condition score (BCS) during prepartum. In conclusion, the occurrence of uterine diseases during the transitional period is related to the BCS in the prepartum period, besides serum NEFA and calcium concentrations at partum.

O objetivo do estudo foi identificar possíveis metabólitos no período de transição capazes de predizer a ocorrência de enfermidades uterinas puerperais em vacas leiteiras (Holandês X Gir). A hipótese foi de que, semelhante ao observado em vacas Holandesas, o balanço energético negativo (BEN) aumentaria a incidência de enfermidades uterinas. Foram utilizadas 34 vacas lactantes com variação entre ½HG, »HG, ¾HG e 5/8HG de composição genética. Os momentos avaliados foram estabelecidos como pré-parto (-60d e -30d), parto e pós-parto (+14d, +21d e +35d) e as fêmeas divididas em dois grupos, com e sem enfermidades uterinas. O BEN foi estabelecido com base nas concentrações de ácidos graxos não-esterificados (NEFA) e beta-hidroxibutirato (BHBA) no parto e 14 dias pós-parto. Observou-se que cerca de 1/3 das vacas mestiças passaram por BEN caracterizado por aumento significativo de NEFA e sem aumento de BHBA, porém em intensidade menor do que o observado em vacas Holandesas. Verificou-se diferença significativa do escore de condição corporal (ECC) no pré-parto e das concentrações de NEFA e de cálcio no parto, entre vacas com e sem enfermidades uterinas. Conclui-se que o ECC no pré-parto e as concentrações de NEFA e cálcio no parto se relacionam com a ocorrência de enfermidades uterinas.

Perfil bioquímico sérico de tilápias do Nilo (Oreochromis niloticus) criadas em tanques rede durante as estações do verão e inverno / Serum biochemical profile of Nile tilapias (Oreochromis niloticus) bred in net cages during summer and winter

Background: The growth of fish farming in Brazil has been noticeable over the past few years, and the Nile tilapia (Oreochromis niloticus) is the most bred species in the country. Studies on the physiology of these animals are necessary so that the production continues to grow and to improve its rates. However, very discrepant data are still found in the area of blood biochemistry. The aim of this study was to evaluate the physiological variations in serum concentrations of proteins, metabolites, electrolytes and serum enzymes in Nile tilapias bred in net cages in Cachoeira Dourada, state of Minas Gerais, Brazil, during summer and winter. Materials, Methods & Results: During the year of 2016, samples from 100 Nile tilapias were collected during summer (January and February) and samples from 100 Nile tilapias were also collected during winter (July and August). Only one blood sample was collected by caudal venipuncture of each fish, just before the slaughter, always in the morning. The serum samples obtained were then analyzed for serum concentrations of proteins, metabolites, electrolytes, lipids and serum enzymes. Serum concentrations of total proteins, globulins, triglycerides, high density lipoprotein (HDL), alkaline phosphatase, sodium and chloride were significantly higher during winter in comparison to summer, while the serum concentration of uric acid was significantly lower during winter in comparison to summer. Discussion: Due to the lower temperature of water during winter, tilapias reduced food intake and became more susceptible to diseases, which led to an increase in circulating globulins. In that same season, there was an increase in serum concentrations of sodium and chloride as a result of adding sodium chloride to the water in order to stimulate mucus secretion on the fishes skin and gills to prevent pathologies. Triglycerides, HDL, and uric acid are considered indicators of nutritional status of animals.[...](AU)

Eficácia anti-helmíntica da ivermectina em equinos: exames coproparasitológicos e hematológicos / Antihelminthic efficacy of ivermectin in horses: coproparasitological and hematological exams

O objetivo do presente estudo foi avaliar a eficácia da ivermectina a 2% em equinos, através de exames coproparasitológicos e hematológicos. O experimento foi realizado em uma fazenda no município de Uberlândia, MG. Foram utilizados 24 equinos, de três a oito anos, divididos em dois grupos, conforme valores dos OPG. Contagens de OPG e coproculturas foram realizadas nos dias D0, D+7 e D+14. Os animais foram tratados com a formulação pasta a base de ivermectina 2%, na dose de 200 mcg/kg, via oral. Amostras de sangue foram coletadas nos dias D0, D+7 e D+14 para avaliação dos parâmetros hematológicos. A presença de ovos, característicos da família Strongylidae e identificação de larvas características de ciatostomíneos demonstrou o parasitismo dos animais por pequenos estrôngilos. Nos D+7 e D+14 houve a redução significativa do OPG no grupo tratado com eficácia de 99,5 e 99,7%, respectivamente. Observou-se tendência do quadro eritrocitário manter-se próximo aos limites inferiores e discreta leucocitose com neutrófilos próximos dos valores superiores. Pode-se inferir que a ivermectina é eficaz contra os ciatostomíneos e alterações hematológicas significativas não foram observadas.(AU)

The aim of the present study was to evaluate the efficacy of ivermectin at 2% in horses, through coproparasitological and hematological exams. The experiment was performed at a farm in the city of Uberlândia, Minas Gerais State. Twenty-four horses were used, ranging from 3 to 8 years of age, divided into 2 groups according to EPG values. EPG counts and coprocultures were performed on days D0, D+7 and D+14. The animals were treated with the ivermectin 2%-based paste formulation at a dose of 200 mcg/kg, orally. Blood samples were collected on days D0, D+7 and D+14 for evaluation of haematological parameters. The presence of eggs that are characteristic of the Strongylidae family and the identification of larvae that are characteristic of cyathostomins demonstrated the parasitism of the animals by small strongyles. At D+7 and D+14 there was a significant reduction of EPG in the treated group with an efficacy of 99.5 and 99.7%, respectively. A tendency of the erythrocyte framework to remain close to the lower limits was observed, besides a slight leukocytosis, with neutrophils close to the highest values. It can be inferred that ivermectin is effective against cyastostomines, and no significant haematological changes were observed.(AU)

Effects of age and sex on blood biochemistry of dorper lambs / Influência das faixas etárias e do sexo sobre variáveis bioquímicas sanguíneas de cordeiros da raça dorper

The purpose of this study was to evaluate the physiological variations and influence of age group and sex on biochemical variables of 32 male and female Dorper sheep at the age of 15 to 121 days. Four blood samples were collected from each animal, at monthly intervals, totaling 128 samples. Biochemical variables determined in each sample were total protein (TP), albumin (ALB), globulin (GLB), albumin/globulin ratio (A/G), glucose (GLU), triglycerides (TG), total cholesterol (tCHOL), high density cholesterol carrying lipoproteins (HDL-C), low density cholesterol carrying lipoproteins (LDL-C), very low density cholesterol carrying lipoproteins (VLDL-C), urea, creatinine (CRE), total calcium (tCa), ionized calcium (iCa), phosphorus (P), calcium/phosphorus ratio (Ca/P), magnesium (Mg), sodium (Na), potassium (K), chloride (Cl), alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT) and alkaline phosphatase (ALP). The results were obtained in four moments according to age: M1 - sheep from 15 to 30 days, M2 - sheep from 45 to 61 days, M3 - sheep from 74 to 90 days and M4 - sheep from 105 to 121 days. Comparing the values of the biochemical variables among the four age groups studied a significant difference (p 0.05) was observed in the concentrations of all the variables evaluated except for Na, Cl and ALT. Sex did not influence (p > 0.05) the values of the biochemical variables analyzed. Based on the results, it is concluded that the agegroups exert a significant influence on the majority of blood biochemical variables of Dorper sheep from15 to 121 days of age.(AU)

Objetivou-se com o presente estudo avaliar as variações fisiológicas e influência das faixas etárias e sexo nas variáveis bioquímicas sanguíneas de 32 ovinos machos e fêmeas da raça Dorper dos 15 aos 121 dias de idade. Foram realizadas quatro coletas de sangue em cada animal, em intervalos mensais, totalizando 128 amostras. As variáveis bioquímicas determinadas em cada amostra foram proteína total (PT), albumina (ALB), globulinas (GLOB), relação albumina/globulina (A/G), glicose (GLI), triglicérides (TRI), colesterol total (CHOLt), lipoproteínas de alta densidade carreadoras de colesterol (HDL-C), lipoproteínas de baixa densidade carreadoras de colesterol (LDL-C), lipoproteínas de muito baixa densidade carreadoras de colesterol (VLDL-C), ureia, creatinina (CREA), cálcio total (Cat), cálcio ionizado (Cai), fósforo (P), relação cálcio/fósforo (Ca/P), magnésio (Mg), sódio (Na), potássio (K), cloreto (Cl), alanina aminotransferase (ALT), aspartato aminotransferase (AST), gama glutamiltransferase (GGT) e fosfatase alcalina (FAL). Os resultados foram obtidos em quatro momentos de acordo com a idade: M1 - ovinos de 15 a 30 dias, M2 - ovinos de 45 a 61 dias, M3 - ovinos de 74 a 90 dias e M4 - ovinos de 105 a 121 dias. Comparados os valores das variáveis bioquímicas entre as quatro faixas etárias estudadas observou-se diferença significativa (p < 0,05) nas concentrações de todas as variáveis avaliadas com exceção de Na, Cl e ALT. O sexo não influenciou (p > 0,05) os valores dasvariáveis bioquímicas sanguíneas analisadas. Com base nos resultados conclui-se que as faixas etáriasexercem influência significativa na maioria das variáveis bioquímicas sanguíneas de ovinos da raçaDorper dos 15 aos 121 dias de idade.(AU)