RESUMO
Campylobacter upsaliensis is an enteropathogenic bacterium in animals, and is also rarely isolated from humans, where it can cause enteritis and bacteremia. This report describes the first case of isolation of C. upsaliensis from an infected giant hepatic cyst. This bacterium could not be cultured from abscess punctuate in a usual Campylobacter-selection medium (charcoal cefoperazone deoxycholate agar medium), because of high concentration of cefoperazone as a selection agent. It could not identified by matrix-assisted laser desorption ionization-time of flight mass spectrum. Rather, it was identified as C. upsaliensis by whole genome sequencing, including by multilocus sequence typing.
Assuntos
Infecções por Campylobacter/diagnóstico , Campylobacter upsaliensis/isolamento & purificação , Cistos/diagnóstico , Abscesso Hepático/diagnóstico , Idoso , Antibacterianos/administração & dosagem , Infecções por Campylobacter/microbiologia , Infecções por Campylobacter/terapia , Campylobacter upsaliensis/genética , Catéteres , Cefoperazona/administração & dosagem , Cistos/microbiologia , Cistos/terapia , DNA Bacteriano/genética , DNA Bacteriano/isolamento & purificação , Quimioterapia Combinada , Humanos , Fígado/diagnóstico por imagem , Fígado/microbiologia , Abscesso Hepático/microbiologia , Abscesso Hepático/terapia , Masculino , Tipagem de Sequências Multilocus , Paracentese/instrumentação , Sulbactam/administração & dosagem , Tomografia Computadorizada por Raios X , Resultado do TratamentoRESUMO
Multidrug-resistant Streptococcus pneumoniae strains were isolated from blood and sputum of a patient with disseminated intravascular coagulation in Sapporo city, Japan. These antibiograms were only susceptible to vancomycin, linezolid, daptomycin, some carbapenems, and some fluoroquinolones. Identical antibiograms, serotypes (19F), and sequence types (ST10017) suggested a shared origin of these isolates. Only one ST10017 strain has been isolated in the same city in Japan previously (2014), and the 2014 isolate is still susceptible to macrolides. The whole genome of the blood-derived isolate was sequenced. The strain harbored resistance mutations in parC, gyrA, pbp1a, pbp2a, pbp2b, and pbp2x, and harbored the resistance genes, ermB and tetM. The nucleotide sequences of parC and pbp2x genes of strain MDRSPN001 were clearly different from those of other S. pneumoniae strains and were similar to those of oral streptococci strains. These findings suggest that strain MDRSPN001 has been rapidly and drastically evolving multidrug resistance by gene replacement and accumulation of genes originating from other strains, such as oral streptococci, Streptococcus mitis.
Assuntos
Antibacterianos/farmacologia , Coagulação Intravascular Disseminada/tratamento farmacológico , Farmacorresistência Bacteriana Múltipla/genética , Infecções Pneumocócicas/tratamento farmacológico , Streptococcus pneumoniae/genética , Antibacterianos/uso terapêutico , Coagulação Intravascular Disseminada/diagnóstico por imagem , Coagulação Intravascular Disseminada/microbiologia , Feminino , Genoma Bacteriano/genética , Humanos , Japão , Testes de Sensibilidade Microbiana , Pessoa de Meia-Idade , Infecções Pneumocócicas/diagnóstico por imagem , Infecções Pneumocócicas/microbiologia , Sorogrupo , Streptococcus pneumoniae/efeitos dos fármacos , Streptococcus pneumoniae/isolamento & purificação , Tomografia Computadorizada por Raios X , Sequenciamento Completo do GenomaRESUMO
Invasive infection of multidrug-resistant Streptococcus pneumoniae is a serious clinical concern. Here, we report the complete genome sequence of a multidrug-resistant S. pneumoniae serotype 19F strain isolated from a patient with an invasive infection in Sapporo, Japan.