Mandibular anterior intrusion using miniscrews for skeletal anchorage: A 3-dimensional finite element analysis.

INTRODUCTION: Deepbites can be corrected by intrusion of mandibular anterior teeth. Direct anchorage with miniscrews simplifies complex tooth movements; however, few studies have reported their use for mandibular anterior intrusion. The purpose of this study was to evaluate, by means of the finite element method, initial tooth displacement and periodontal stress distribution using various mandibular anterior intrusion mechanics. Miniscrews were used as skeletal anchorage devices. METHODS: Cone-beam computed tomography scans were used for 3-dimensional reconstruction of the mandible and the mandibular anterior dentition. Models included the 4 incisors with or without the canines. After all surrounding periodontal and bony structures were determined brackets, segmental archwires, and miniscrews were added. Finite element studies were performed to assess initial tooth displacement and periodontal stress distribution with multiple intrusion force vectors. Changes in the location of the miniscrews and loading points on the archwire created 14 scenarios. RESULTS: Minimum buccolingual displacements, a uniform distribution of periodontal stress, and overall group intrusion for both 4-tooth and 6-tooth scenarios were best achieved when applying distointrusive vectors. The highest peaks of periodontal stress were observed when the force was directed at the corners of the segmental archwire. It was found that, in addition to distointrusive vectors, 4 loading points on the archwire were necessary for pure intrusion and uniform distribution of periodontal stress in the 6-tooth scenarios. CONCLUSIONS: The simulations in this study suggest that group intrusion of all 6 mandibular anterior teeth might be achieved by applying distointrusive vectors. Inserting a pair of miniscrews distal to the canine roots, 1 screw per side, and directing 4 loading points on the archwire generates uniform periodontal stress distribution and minimum buccolingual displacements. Local conditions, such as narrow bone width and attached gingiva level, play significant roles in the clinical viability of the proposed virtual scenarios.

Chlorogenic acid facilitates root hair formation in lettuce seedlings.

Root hairs, which arise from root epidermal cells, are tubular structures that increase the efficiency of water absorption and nutrient uptake. A low pH (pH 4) medium induced root hair formation in lettuce (Lactuca sativa L.) seedlings, and the decapitation of shoots inhibited root hair formation. The addition of shoot extract to the medium restored root hair formation in the decapitated lettuce seedlings. These results suggest that factors essential to the formation of root hairs may be present in the shoot. We purified one factor from the shoot that facilitates root hair formation. This factor was identified as chlorogenic acid (CGA), a common polyphenol in higher plants. The presence of exogenous CGA in the medium induced root hair formation in decapitated lettuce seedlings at pH 4.0 and in intact lettuce seedlings at pH 6.0. The optimum concentration of CGA for root hair formation was identified as 10(-5) M. Decapitation of the shoots reduced the CGA content in the roots to approximately one-third that in intact plants. Application of the CGA biosynthesis inhibitor L-alpha-aminooxy-beta-phenylpropionic acid (AOPP, 10(-6) M) to intact seedlings grown at pH 4.0 reduced both the CGA content of the roots and the total amount of root hairs. The addition of exogenous CGA restored root hair formation in intact seedlings treated with AOPP. These results suggest that CGA is essential for root hair formation in lettuce seedlings.