RESUMO
Acetylcholinesterase (AChE) inhibitors are still an important option for managing symptoms of mild to moderate Alzheimer's disease. In this study, we aimed to evaluate the potential in vitro AChE inhibitory activity of two Argentinian endemic Solanaceae species, Jaborosa bergii and J. runcinata. UHPLC-DAD-HRMS metabolite profiling revealed the presence of withanolides in the active CH2Cl2 subextracts. Their fractionation led to the isolation and identification of two known spiranoid withanolides from J. runcinata and three new withanolides with a skeleton similar to that of trechonolide-type withanolides from J. bergii. The known compounds showed moderate AChE inhibitory activity, while the new ones were inactive.
Assuntos
Inibidores da Colinesterase , Solanaceae , Vitanolídeos , Vitanolídeos/farmacologia , Vitanolídeos/química , Vitanolídeos/isolamento & purificação , Inibidores da Colinesterase/farmacologia , Inibidores da Colinesterase/química , Solanaceae/química , Argentina , Acetilcolinesterase/metabolismo , Acetilcolinesterase/efeitos dos fármacos , Estrutura Molecular , Extratos Vegetais/farmacologia , Extratos Vegetais/químicaRESUMO
This paper introduces the lethal, sublethal, and ecotoxic effects of peppermint and palmarosa essential oils (EOs) and their polymeric nanoparticles (PNs). The physicochemical analyses indicated that peppermint PNs were polydisperse (PDI > 0.4) with sizes of 381 nm and loading efficiency (LE) of 70.3%, whereas palmarosa PNs were monodisperse (PDI < 0.25) with sizes of 191 nm and LE of 89.7%. EOs and their PNs were evaluated on the adults of rice weevil (Sitophilus oryzae L.) and cigarette beetle (Lasioderma serricorne F.) and the larvae of Culex pipiens pipiens Say. On S. oryzae and L. serricorne, PNs increased EOs' lethal activity, extended repellent effects for 84 h, and also modified behavioral variables during 24 h. Moreover, EOs and PNs generated toxic effects against C. pipiens pipiens. On the other hand, peppermint and palmarosa EOs and their PNs were not toxic to terrestrial non-target organisms, larvae of mealworm (Tenebrio molitor L.), and nymphs of orange-spotted cockroach (Blaptica dubia S.). In addition, PNs were slightly toxic to aquatic non-target organisms, such as brine shrimp (Artemia salina L.). Therefore, these results show that PNs are a novel and eco-friendly formulation to control insect pests.
Assuntos
Repelentes de Insetos , Inseticidas , Nanopartículas , Óleos Voláteis , Tenebrio , Gorgulhos , Animais , Repelentes de Insetos/farmacologia , Inseticidas/farmacologia , Larva , Óleos Voláteis/químicaRESUMO
Alzheimer's disease is a multifactorial neurodegenerative disorder. Since cholinergic deficit is a major factor in this disease, two molecular targets for its treatment are the acetylcholinesterase (AChE) and the nicotinic acetylcholine receptors (nAChRs). Given that caffeine is a natural compound that behaves as an AChE inhibitor and as a partial agonist of nAChRs, the aim of this work was to synthetize more potent bifunctional caffeine analogs that modulate these two molecular targets. To this end, a theophylline structure was connected to a pyrrolidine structure through a methylene chain of different lengths (3 to 7 carbon atoms) to give compounds 7-11 All caffeine derivatives inhibited the AChE, of which compound 11 showed the strongest effect. Electrophysiological studies showed that all compounds behave as agonists of the muscle and the neuronal α7 nAChR with greater potency than caffeine. To explore whether the different analogs could affect the nAChR conformational state, the nAChR conformational-sensitive probe crystal violet (CrV) was used. Compounds 9 and 10 conduced the nAChR to a different conformational state comparable with a control nAChR desensitized state. Finally, molecular docking experiments showed that all derivatives interacted with both the catalytic and anionic sites of AChE and with the orthosteric binding site of the nAChR. Thus, the new synthetized compounds can inhibit the AChE and activate muscle and α7 nAChRs with greater potency than caffeine, which suggests that they could be useful leaders for the development of new therapies for the treatment of different neurologic diseases. SIGNIFICANCE STATEMENT: In this work we synthetized caffeine derivatives which can inhibit acetylcholinesterase and activate both muscle and α7 nicotinic acetylcholine receptors (nAChRs) with higher potency than caffeine. These analogs can be divided into two groups: a non-desensitizing and a desensitizing nAChR group. From the nAChR non-desensitizing group, we propose compound 11 as the most interesting analog for further studies since it inhibits acetylcholinesterase with the highest potency and activates the nAChRs in the picomolar range without inducing receptor desensitization.
Assuntos
Cafeína/análogos & derivados , Cafeína/síntese química , Receptor Nicotínico de Acetilcolina alfa7/agonistas , Receptor Nicotínico de Acetilcolina alfa7/metabolismo , Animais , Cafeína/metabolismo , Cafeína/farmacologia , Electrophorus , Células HEK293 , Humanos , Simulação de Acoplamento Molecular/métodos , Estrutura Secundária de Proteína , Torpedo , Receptor Nicotínico de Acetilcolina alfa7/químicaRESUMO
To date, Alzheimer's disease is the most alarming neurodegenerative disorder worldwide. This illness is multifactorial in nature and cholinesterase inhibitors have been the ones used in clinical treatments. In this context, many of these drugs selectively inhibit the acetylcholinesterase enzyme interacting in both the active site and the peripheric anionic site. Besides, some agents have exhibited extensive benefits being able to co-inhibit butyrylcholinesterase. In this contribution, a strategy previously explored by numerous authors is reported; the synthesis of hybrid cholinesterase inhibitors. This strategy uses a molecule of recognized high inhibitory activity (tacrine) together with a steroidal alkaloid of natural origin using different connectors. The biological assays demonstrated the improvement in the inhibitory activity compared to the alkaloidal precursor, together with the reinforcement of the interactions in multiple sites of the enzymatic cavity. This strategy should be explored and exploited in this area. Docking and molecular dynamic studies were performed to explain enzyme-ligand interactions, assisting a structure-activity relationship analysis.
Assuntos
Alcaloides/farmacologia , Produtos Biológicos/farmacologia , Inibidores da Colinesterase/farmacologia , Desenho de Fármacos , Esteroides/farmacologia , Acetilcolinesterase/metabolismo , Alcaloides/síntese química , Alcaloides/química , Animais , Produtos Biológicos/síntese química , Produtos Biológicos/química , Butirilcolinesterase/metabolismo , Inibidores da Colinesterase/síntese química , Inibidores da Colinesterase/química , Relação Dose-Resposta a Droga , Electrophorus , Cavalos , Estrutura Molecular , Esteroides/síntese química , Esteroides/química , Relação Estrutura-AtividadeRESUMO
Nowadays, the importance of computational methods in the design of therapeutic agents in a more efficient way is indisputable. Particularly, these methods have been important in the design of novel acetylcholinesterase enzyme inhibitors related to Alzheimer's disease. In this sense, in this report a computational model of linear prediction of acetylcholinesterase inhibitory activity of steroids and triterpenes is presented. The model is based in a correlation between binding energies obtained from molecular dynamic simulations (after docking studies) and [Formula: see text] values of a training set. This set includes a family of natural and semi-synthetic structurally related alkaloids reported in bibliography. These types of compounds, with some structural complexity, could be used as building blocks for the synthesis of many important biologically active compounds Therefore, the present study proposes an alternative based on the use of conventional and easily accessible tools to make progress on the rational design of molecules with biological activity.
Assuntos
Acetilcolinesterase/química , Inibidores da Colinesterase/farmacologia , Esteroides/farmacologia , Triterpenos/farmacologia , Domínio Catalítico , Humanos , Simulação de Acoplamento Molecular , Simulação de Dinâmica Molecular , Conformação Proteica , Relação Estrutura-AtividadeRESUMO
Plants of the Amaryllidaceae family are well-known (not only) for their ornamental value but also for the alkaloids that they produce. In this report, the first phytochemical study of Clinanthus genus was carried out. The chemical composition of alkaloid fractions from Clinanthus microstephium was analyzed by GC/MS and NMR. Seven known compounds belonging to three structural types of Amaryllidaceae alkaloids were identified. An epimeric mixture of a haemanthamine-type compound (6-hydroxymaritidine) was tested as an inhibitor against acetyl- and butyrylcholinesterase enzymes (AChE and BChE, respectively), two enzymes relevant in the treatment of Alzheimer's disease, with good results. Structure-activity relationships through molecular docking studies with this alkaloid and other structurally related compounds were discussed.
Assuntos
Alcaloides/química , Alcaloides de Amaryllidaceae/química , Amaryllidaceae/química , Inibidores da Colinesterase/química , Fenantridinas/química , Acetilcolinesterase/química , Acetilcolinesterase/metabolismo , Alcaloides/metabolismo , Alcaloides/farmacologia , Amaryllidaceae/metabolismo , Sítios de Ligação , Butirilcolinesterase/química , Butirilcolinesterase/metabolismo , Domínio Catalítico , Inibidores da Colinesterase/isolamento & purificação , Inibidores da Colinesterase/metabolismo , Inibidores da Colinesterase/farmacologia , Cromatografia Gasosa-Espectrometria de Massas , Concentração Inibidora 50 , Simulação de Acoplamento Molecular , Extratos Vegetais/química , Relação Estrutura-AtividadeRESUMO
A series of thirty-three alkynyl and ß-ketophosphonates were evaluated for their in vitro acetyl- and butyryl-cholinesterase (AChE and BChE) inhibitory activities using Ellman's spectrophotometric method. None of the examined compounds inhibited AChE activity at tested concentrations while twenty-nine of them showed significant and selective inhibition of BChE with IC50 values between 38.60⯵M and 0.04⯵M. In addition, structure-activity relationships were discussed. The most effective inhibitors were the dibutyl o-methoxyphenyl alkynylphosphonate 3dc and dibutyl o-methoxyphenyl ß-ketophosphonate 4dc. Activities of most potent compounds were also compared with a commercial organophosphorus compound. These results could inspire the design of new inhibitors with stronger activity against BChE.
Assuntos
Alcinos/farmacologia , Butirilcolinesterase/metabolismo , Inibidores da Colinesterase/farmacologia , Organofosfonatos/farmacologia , Acetilcolinesterase/metabolismo , Alcinos/síntese química , Alcinos/química , Animais , Inibidores da Colinesterase/síntese química , Inibidores da Colinesterase/química , Relação Dose-Resposta a Droga , Electrophorus , Cavalos , Estrutura Molecular , Organofosfonatos/síntese química , Organofosfonatos/química , Relação Estrutura-AtividadeRESUMO
The development of hybrids from natural products is a promising strategy for drug discovery. In cancer therapy, there is a need to discover novel agents that can induce apoptosis in cancer cells. To contribute to this field of interest, we investigated the effect of a synthetic hybrid from cativic acid and caffeic acid (5) on viability, proliferation, and apoptosis in human neuroblastoma cells (IMR-32). Three hybrids were prepared via Mitsunobu esterification from 17-hydroxycativic acid (1) and natural phenols. Cell viability was analyzed by MTT assay. SYTOX green and LDH leakage were used to determine the cytotoxic effect. Caspase-3 activity, cell cycle phases, and proliferation were analyzed in order to characterize the biological effects of hybrid 5. The mitogen-activated protein kinase (MAPK) status was evaluated for elucidating the potential mechanisms involved in hybrid 5 effect. Hybrid 5 reduced the viability of IMR-32 cells in a time- and concentration-dependent manner (IC50 = 18.0 ± 1.3 µM) as a result of its antiproliferative effect through changes in the cell cycle distribution and induction of apoptosis associated with activation of caspase-3. Exposure to 5 triggered ERK1/2 activation and nuclear translocation. Hybrid 5 also promoted an increase in nuclear localization of the transcription factor c-Jun. Inhibition of ERK1/2 and JNK potentiated 5-induced inhibition of IMR-32 viability. Hybrid 5 displays cell growth inhibition by promoting cell cycle arrest and apoptosis, through ERK1/2 and JNK participation.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Ácidos Cafeicos/farmacologia , Diterpenos/farmacologia , Antineoplásicos Fitogênicos/química , Apoptose/efeitos dos fármacos , Ácidos Cafeicos/química , Caspase 3/metabolismo , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Diterpenos/química , Grindelia/química , Humanos , Proteínas Quinases JNK Ativadas por Mitógeno/metabolismo , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Neuroblastoma/tratamento farmacológico , Sinais de Localização Nuclear/efeitos dos fármacosRESUMO
The investigation of natural products in medicinal chemistry is essential today. In this context, acetylcholinesterase (AChE) inhibitors comprise one type of the compounds most actively studied in the search for an effective treatment of symptoms of Alzheimer's disease. This work describes the isolation of a natural compound, solanocapsine, the preparation of its chemical derivatives, the evaluation of AChE inhibitory activity, and the structure-activity analysis of relevant cases. The influence of structural variations on the inhibitory potency was carefully investigated by modifying different reactive parts of the parent molecule. A theoretical study was also carried out into the binding mode of representative compounds to the enzyme through molecular modeling. The biological properties of the series were investigated. Through this study valuable information was obtained of steroidal alkaloid-type compounds as a starting point for the synthesis of AChE inhibitors.
Assuntos
Acetilcolinesterase/metabolismo , Alcaloides/farmacologia , Inibidores da Colinesterase/síntese química , Inibidores da Colinesterase/farmacologia , Simulação de Acoplamento Molecular , Esteroides/farmacologia , Alcaloides/síntese química , Alcaloides/química , Animais , Inibidores da Colinesterase/química , Relação Dose-Resposta a Droga , Electrophorus , Estrutura Molecular , Esteroides/síntese química , Esteroides/química , Relação Estrutura-AtividadeRESUMO
Alzheimer's disease (AD) is a neurodegenerative disorder associated with memory impairment and cognitive deficit. Most of the drugs currently available for the treatment of AD are acetylcholinesterase (AChE) inhibitors. Plants of the Amaryllidaceae family are known to synthesize alkaloids, which have shown AChE inhibitory activity. Habranthus tubispathus and H. jamesonii are two Amaryllidaceae that can be found growing wild to the southwest of Buenos Aires in Argentina. Acetyl- and butyrylcholinesterase inhibition was observed for the extracts obtained from bulbs of H. tubispathus and bulbs and aerial parts of H. jamesonii. The strongest cholinesterase inhibition was observed for the alkaloid extract obtained from the aerial parts for H. jamesonii (AChE IC50 = 0.7 microg/mL; BChE IC50 = 6.7 microg/mL). The AChE inhibition observed for H. jamesonii could be explained by the presence of galanthamine and sanguinine, two potent AChE inhibitors. The levels of lycorine and hippeastidine, moderate AChE inhibitors, observed in the bulbs of H. tubispathus could be responsible for the significant AChE inhibition observed. The alkaloids present in these Amaryllidaceae were identified by means of GC-MS analysis. In the case of H. tubispathus, hippeastidine and 3-O-demethylhippeastidine, were isolated and completely characterized by 1H and 13C NMR spectroscopy.
Assuntos
Alcaloides de Amaryllidaceae/isolamento & purificação , Inibidores da Colinesterase/isolamento & purificação , Alcaloides de Amaryllidaceae/química , Butirilcolinesterase/efeitos dos fármacos , Cromatografia Gasosa-Espectrometria de Massas , Espectroscopia de Ressonância MagnéticaRESUMO
A bioassay-guided phytochemical analysis of the ethanolic extract of Grindelia argentina Deble & Oliveira-Deble (Asteraceae) allowed the isolation of a known flavone, hispidulin, and three new oleanane-type saponins, 3-O-ß-D-xylopyranosyl-(1â3)-ß-D-glucopyranosyl-2ß,3ß,16α,23-tetrahydroxyolean-12-en-28-oic acid 28-O-ß-D-xylopyranosyl-(1â2)-ß-D-apiofuranosyl-(1â3)-ß-D-xylopyranosyl-(1â3)-α-L-rhamnopyranosyl-(1â2)-α-L-arabinopyranosyl ester (2), 3-O-ß-D-glucopyranosyl-2ß,3ß,23-trihydroxyolean-12-en-28-oic acid 28-O-ß-D-xylopyranosyl-(1â2)-ß-D-apiofuranosyl-(1â3)-ß-D-xylopyranosyl-(1â3)-α-L-rhamnopyranosyl-(1â2)-α-L-arabinopyranosyl ester, (3) and 3-O-ß-D-xylopyranosyl-(1â3)-ß-D-glucopyranosyl-2ß,3ß,23-trihydroxyolean-12-en-28-oic acid 28-O-ß-D-xylopyranosyl-(1â2)-ß-D-apiofuranosyl-(1â3)-ß-D-xylopyranosyl-(1â3)-α-L-rhamnopyranosyl-(1â2)-α-L-arabinopyranosyl ester (4), named grindeliosides A-C, respectively. Their structures were determined by extensive 1D- and 2D-NMR experiments along with mass spectrometry and chemical evidence. The isolated compounds were evaluated for their inhibitory activities against LPS/IFN-γ-induced NO production in RAW 264.7 macrophages and for their cytotoxic activities against the human leukemic cell line CCRF-CEM and MRC-5 lung fibroblasts. Hispidulin markedly reduced LPS/IFN-γ-induced NO production (IC50 51.4â µM), while grindeliosides A-C were found to be cytotoxic, with grindelioside C being the most active against both CCRF-CEM (IC50 4.2±0.1â µM) and MRC-5 (IC50 4.5±0.1â µM) cell lines.
Assuntos
Grindelia/química , Óxido Nítrico/biossíntese , Saponinas/farmacologia , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos , Fibroblastos/efeitos dos fármacos , Fibroblastos/metabolismo , Humanos , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Estrutura Molecular , Saponinas/química , Saponinas/isolamento & purificação , Relação Estrutura-AtividadeRESUMO
Disulfated and trisulfated steroids have been synthesized from cholesterol and their acetylcholinesterase inhibitory activity has been evaluated. In our studies we have found that the activity was not only dependent on the location of the sulfate groups but on their configurations. 2ß,3α,6α-trihydroxy-5α-cholestan-6-one trisulfate (18) was the most active steroid with an IC50 value of 15.48 µM comparable to that of 2ß,3α-dihydroxy-5α-cholestan-6-one disulfate (1). Both compounds were found to be less active than the reference compound eserine. The butyrylcholinesterase activity of 1 and 18 was one magnitude lower than that against acetylcholinesterase revealing a selective inhibitor profile.
Assuntos
Acetilcolinesterase/metabolismo , Inibidores da Colinesterase/síntese química , Inibidores da Colinesterase/farmacologia , Esteroides/síntese química , Esteroides/farmacologia , Sulfatos/química , Animais , Técnicas de Química Sintética , Inibidores da Colinesterase/química , Hidroxilação , Concentração Inibidora 50 , Esteroides/química , Relação Estrutura-Atividade , TorpedoRESUMO
Disodium 2ß,3α-dihydroxy-5α-cholestan-6-one disulfate (8) has been synthesized using cholesterol (1) as starting material. Sulfation was performed using trimethylamine-sulfur trioxide complex in dimethylformamide as the sulfating agent. The acetylcholinesterase inhibitory activity of compound 8 was evaluated and compared to that of disodium 2ß,3α-dihydroxy-5α-cholestane disulfate (10) and diols 7 and 9. Compounds 8 and 10 were active with IC(50) values of 14.59 and 59.65 µM, respectively. Diols 7 and 9 showed no inhibitory activity (IC(50)>500 µM).
Assuntos
Acetilcolinesterase/metabolismo , Esteroides/síntese química , Esteroides/farmacologia , Ativação Enzimática/efeitos dos fármacos , Cinética , Espectroscopia de Ressonância Magnética , Esteroides/químicaRESUMO
BACKGROUND: Plant essential oils have been recognised as an important natural source of insecticide. This study analysed the chemical constituents and bioactivity of essential oils that were isolated via hydrodistillation from Origanum vulgare L. (oregano) and Thymus vulgaris L. (thyme) against eggs, second instar and adults of Nezara viridula (L.). RESULTS: The major component of oregano was p-cymene, and, for thyme, thymol. The ovicidal activity was tested by topical application; the essential oil from thyme was more effective. The fumigant activity was evaluated in an enclosed chamber; the LC50 values for oregano were 26.8 and 285.6 µg mL(-1) for nymphs and adults respectively; for thyme they were 8.9 µg mL(-1) for nymphs and 219.2 µg mL(-1) for adults. To evaluate contact activity, a glass vial bioassay was used; the LC50 values for oregano were 1.7 and 169.2 µg cm(-2) for nymphs and adults respectively; for thyme they were 3.5 and 48.8 µg cm(-2) respectively. The LT50 analyses for contact and fumigant bioassays indicated that thyme was more toxic for nymphs and adults than oregano. Both oils produced repellency on nymphs and adults. CONCLUSION: These results showed that the essential oils from O. vulgare and T. vulgaris could be applicable to the management of N. viridula.
Assuntos
Heterópteros , Inseticidas , Óleos Voláteis/química , Origanum/química , Thymus (Planta)/química , Animais , Feminino , Fumigação , Repelentes de Insetos , Óvulo , Testes de ToxicidadeRESUMO
The objective of the current study was to determine the chemical constituents, ovicidal activity, fumigant, contact toxicity and repellent effects of essential oils isolated by hydrodistillation from Aloysia polystachya and A. citriodora against eggs and second instar nymphs of Nezara viridula. The major components were carvone (83.5%) for A. polystachya, and citronellal (51.3%) and sabinene (22.9%) for A. citriodora. The ovicidal activity of both oils was tested by topical application at different concentrations ranging from 1.2 to 12.5 microg/egg; all concentrations had a toxic effect. Data probit analysis showed that the LC50 value for A. polystachya was 2.3 microg/egg and for A. citriodora 1.9 microg/egg. The fumigant activity was evaluated in an enclosed chamber. The toxicity increased with concentration from 11 to 176 microg/mL air, and with exposure times from 1 to 48 h. The LC50 values for A. polystachya and A. citriodora were 29.9 and 13.5 microg/mL air 24 h after treatment, respectively. To evaluate contact activity a glass-vial bioassay was used. The toxicity increased with concentration from 2.8 to 45 microg/cm2 and with exposure time from 1 to 48 h. The LC50 for A. polystachya was 3.4 microg/cm2 and for A. citriodora 8.1 microg/cm2. The repellency bioassay demonstrated that both oils were active at the highest concentration (2.6 and 5.3 microg/mL air) and neutral at 1.3 microg/mL air. These results show that the essential oils from Aloysia polystachya and A. citriodora could be applicable to the management of populations of Nezara viridula.
Assuntos
Hemípteros/efeitos dos fármacos , Repelentes de Insetos/farmacologia , Inseticidas/farmacologia , Óleos Voláteis/farmacologia , Óleos de Plantas/farmacologia , Verbenaceae/química , Animais , Repelentes de Insetos/química , Inseticidas/química , Ninfa/efeitos dos fármacos , Óleos Voláteis/química , Óleos de Plantas/químicaRESUMO
The essential oil from aerial parts (stems and leaves) of Atriplex undulata (Moq) D. Dietr. (Chenopodiaceae) has been studied for its in vitro antioxidant activity. The chemical composition of the oil obtained by hydrodistillation was determined by GC and GC-MS. The major constituents were p-acetanisole (28.1%), beta-damascenone (9.3%), beta-ionone (5.1%), viridiflorene (4.7%) and 3-oxo-alpha-ionol (2.2%). The antioxidant activity of the oil was determined by two methods: Crocin bleaching inhibition (Krel = 0.72 +/- 0.15) and scavenging of the DPPH radical (IC50 = 36.2 +/- 1.6 microg/mL). The presence of active compounds like p-acetanisole, carvone, vanillin, 4-vinylguaiacol, guaiacol, terpinen-4-ol and alpha-terpineol could explain the antioxidant activity observed for this oil.
Assuntos
Antioxidantes/química , Atriplex/química , Óleos Voláteis/química , Óleos de Plantas/química , Compostos de Bifenilo/química , Picratos/química , Folhas de Planta/química , Caules de Planta/químicaRESUMO
The essential oils of Schinus areira L. and S. longifolia (Lindl.) Speg. (Anacardiaceae) have been studied for their in vitro anti-acetylcholinesterase and antioxidant activities. The chemical composition of the oils obtained by hydrodistillation was determined by GC-MS. Fruit and leaf oils of S. areira were analyzed separately. The essential oil from S. longifolia elicited marked enzymatic inhibition (IC50 = 20.0 +/- 1.0 microg/mL) and showed radical scavenger activity (IC50 = 25.2 +/- 2.4 microg/mL). The essential oil from S. areira leaves was more active than that of the fruits in both bioassays.
Assuntos
Anacardiaceae/química , Antioxidantes/química , Inibidores da Colinesterase/química , Óleos Voláteis/química , Óleos de Plantas/química , Antioxidantes/farmacologia , Inibidores da Colinesterase/farmacologia , Óleos Voláteis/farmacologia , Folhas de Planta/química , Óleos de Plantas/farmacologiaRESUMO
The essential oil composition from the aerial parts of three Anacardiaceae growing in Bahía Blanca, Argentina was studied by gas chromatography and gas chromatography-mass spectrometry. The essential oils of S. longifolia and S. fasciculata have been studied for the first time. The major constituents were alpha-pinene (46.5%), beta-pinene (15.1%) and alpha-phellandrene (10.1%) for S. longifolia and limonene (10.9%), beta-phellandrene (6.16%) and alpha-phellandrene (5.6%) for S. fasciculata. The major components of the essential oil of S. areira were limonene (28.6%), alpha-phellandrene (10.1%), sabinene (9.2%) and camphene (9.2%) differing from the literature data. The essential oils from S. areira and S. longifolia exhibited a high biotoxicity in a brine shrimp assay with Artemia persimilis.
Assuntos
Magnoliopsida/química , Óleos Voláteis/química , Cromatografia Gasosa-Espectrometria de Massas/métodos , Magnoliopsida/classificação , Óleos Voláteis/isolamento & purificação , Folhas de Planta/química , Caules de Planta/química , Especificidade da EspécieRESUMO
A free radical scavenging activity guided fractionation of the polar extract from roots of Limonium brasiliense (Plumbaginaceae) led to the isolation of five active compounds including: myricetin 3-O-alpha-rhamnopyranoside (1), (-)-epigallocatechin 3-O-gallate (2), (-)-epigallocatechin (3), (+)-gallocatechin (4) and gallic acid (5). These and other chemical constituents are reported for the first time for this species. The characterization of these compounds was achieved by spectroscopic methods (1H NMR, 13C NMR and UV).
Assuntos
Antioxidantes/metabolismo , Raízes de Plantas/metabolismo , Plumbaginaceae/metabolismo , Antioxidantes/isolamento & purificação , Compostos de Bifenilo/química , Cromatografia em Gel , Sequestradores de Radicais Livres/isolamento & purificação , Sequestradores de Radicais Livres/metabolismo , Hidrazinas/química , Espectroscopia de Ressonância Magnética , Picratos , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Raízes de Plantas/química , Plumbaginaceae/química , Quercetina/isolamento & purificação , Quercetina/farmacologiaRESUMO
Five new steroid sulfates, sodium 2beta,3alpha-dihydroxy-5alpha-cholestane 3-sulfate (6), sodium 2beta,3alpha-dihydroxy-5alpha-cholestane 2-sulfate (7), disodium 2beta,3alpha-dihydroxy-5alpha-cholestane disulfate (8), sodium 3alpha-acetoxy-2beta-hydroxy-5alpha-cholestane 2-sulfate (12), and sodium 2beta-acetoxy-3alpha-hydroxy-5alpha-cholestane 3-sulfate (13), have been synthesized starting from 3beta-hydroxy-5alpha-cholestane (1). The synthetic steroids were completely characterized by one-dimensional and two-dimensional NMR and FABMS spectra. Sulfation was performed using triethylamine-sulfur trioxide complex in dimethylformamide as the sulfating agent. The sulfated steroids were comparatively evaluated for their inhibitory effect on the replication of herpes simplex virus type 2 (HSV-2). Compounds 7 and 8 were the most effective in their inhibitory action against HSV-2. The disulfated steroid 8 also proved to be active against DEN-2 and JV.