Chitosan capped ZnO nanoparticles with cell specific apoptosis induction through P53 activation and G2/M arrest in breast cancer cells - In vitro approaches.

Now a days the well-organized strategy to induce apoptosis in cancer chemotherapy is to produce anti-cancer agent without any side effects is in needy. Hence the present investigation was aimed to explore the anticancer potentials of Amorphophallus paeoniifolius reduced zinc nanoparticles capped with chitosan against MCF 7 cell line (breast cancer cells)and studied for its optical and surface charge properties. The size, shape, dispersion and uniform distribution of biosynthesized zincoxide nanoparticle was examined using Field emission scanning electron microscope (FESEM) and Transmission electron microscope (TEM) respectively. The spherical and cubic nanocrystals were found to be lethal against MCF 7 cells on MTT assay at dose dependant manner (20-80 µg/ml) whose IC50 value 42 µg/ml. Bright field light microscopic study showed the apoptotic morphology of treated and control MCF-7 cells. Fluorescence staining A/O:EB and DAPI methods further cleared the chromosome condensation, nuclear fragmentation and confirms the apoptosis induced by Ch-Ap-ZnONPS within IC50 concentrations. Significant cell cycle arrest at particular stage of G2/M was achieved with the nanocomplex treatment at dose dependant manner. Finally, it was observed that the apoptotic gens and protein expressions of MCF-7 cell line were up and down regulation with the treatment of Ch-Ap-ZnONPS when compared to normal cells.

Therapeutic and fertility restoration effects of Ionidium suffruticosum on sub-fertile male albino Wistar rats: effects on testis and caudal spermatozoa.

CONTEXT: Ionidium suffruticosum (L.) Ging (Violaceae) is an important medicinal plant widely used as a herbal traditional medicine in Ayurveda for the treatment of infertility. Currently, little pharmacological information is available on its male fertility properties following prolonged use. OBJECTIVE: To investigate I. suffruticosum leaf extracts for male fertility parameters. MATERIALS AND METHODS: The ethanol lyophilized fraction was administered orally on carbendazim-induced sub-fertility rats (250 mg/kg body weight for 28 days). The effects of fractions on rat's fertility parameters i.e., body and testes weight, sperm motility, sperm vitality, epididymal sperm counts, its morphology, enzyme and antioxidant stress and histopathology were studied and compared with clomiphene citrate. RESULTS: The sub-fertile male rats treated with I. suffruticosum leaf extract increased the body weight of 7 g, testis weight of 97 mg, increased cauda epididymal sperm counts of 34.2 × 106 sperm/mL, motility of sperm 46% and vitality 28% also increased and normal sperm morphology also improved up to 32%. The carbendazim-treated group showed loss in body weight of 33 g, testis weight of 851 mg, decreased epididymal sperm counts of 15 × 106 sperm/mL, with sluggish motility and a highly significant fall in the live sperms of about 57%. DISCUSSION AND CONCLUSION: The leaf fraction of I. suffructicosum increased the testicular weight, spermatogenesis, sperm counts, lessened sperm agglutination, and increased testicular oxidative biomarkers, SOD, and CAT. This study therefore supports the usage of I. suffructicosum in traditional medicine for infertility.