Formulation of Ensulizole with Beta-Cyclodextrins for Improved Sunscreen Activity and Biocompatibility.

In today's context, prolonged exposure to sunlight is widely recognized as a threat to human health, leading to a range of adverse consequences, including skin cancers, premature skin aging, and erythema. To mitigate these risks, preventive actions mainly focus on advocating the application of sunscreen lotions and minimizing direct exposure to sunlight. This research study specifically centered on ensulizole (ENS), a prominent ingredient in sunscreens. The objective was to create inclusion complexes (ICs) with Beta-cyclodextrin (B-CD) and its hydroxypropyl derivatives (H-CD). Using phase solubility measurements, we determined that both B-CD and H-CD form 1:1 stoichiometric ICs with ENS. Proton nuclear magnetic resonance spectral (1H NMR) analysis confirmed that the phenyl portion of ENS is encapsulated within the B-CD cavity. Significant changes in surface morphology were observed during the formation of these ICs compared to ENS and CDs alone. Quantum mechanical calculations were employed to further support the formation of ICs by providing energy data. Particularly, the photostability of the ENS:B-CD ICs remained intact for up to four hours of UV exposure, with no significant alterations in the structure of ENS. Furthermore, comprehensive biocompatibility assessments yielded encouraging results, suggesting the potential application of these inclusion complexes in cosmetics as a UVB sunscreen. In summary, our research underscores the successful creation of inclusion complexes characterized by enhanced photostability and safe biocompatibility.

Adenosine/ß-Cyclodextrin-Based Metal-Organic Frameworks as a Potential Material for Cancer Therapy.

Recently, researchers have employed metal-organic frameworks (MOFs) for loading pharmaceutically important substances. MOFs are a novel class of porous class of materials formed by the self-assembly of organic ligands and metal ions, creating a network structure. The current investigation effectively achieves the loading of adenosine (ADN) into a metal-organic framework based on cyclodextrin (CD) using a solvent diffusion method. The composite material, referred to as ADN:ß-CD-K MOFs, is created by loading ADN into beta-cyclodextrin (ß-CD) with the addition of K+ salts. This study delves into the detailed examination of the interaction between ADN and ß-CD in the form of MOFs. The focus is primarily on investigating the hydrogen bonding interaction and energy parameters through the aid of semi-empirical quantum mechanical computations. The analysis of peaks that are associated with the ADN-loaded ICs (inclusion complexes) within the MOFs indicates that ADN becomes incorporated into a partially amorphous state. Observations from SEM images reveal well-defined crystalline structures within the MOFs. Interestingly, when ADN is absent from the MOFs, smaller and irregularly shaped crystals are formed. This could potentially be attributed to the MOF manufacturing process. Furthermore, this study explores the additional cross-linking of ß-CD with K through the coupling of -OH on the ß-CD-K MOFs. The findings corroborate the results obtained from FT-IR analysis, suggesting that ß-CD plays a crucial role as a seed in the creation of ß-CD-K MOFs. Furthermore, the cytotoxicity of the MOFs is assessed in vitro using MDA-MB-231 cells (human breast cancer cells).

Preparation and Characterization of a Nano-Inclusion Complex of Quercetin with ß-Cyclodextrin and Its Potential Activity on Cancer Cells.

Quercetin (QRC), a flavonoid found in foods and plants such as red wine, onions, green tea, apples, and berries, possesses remarkable anti-inflammatory and antioxidant properties. These properties make it effective in combating cancer cells, reducing inflammation, protecting against heart disease, and regulating blood sugar levels. To enhance the potential of inclusion complexes (ICs) containing ß-cyclodextrin (ß-CD) in cancer therapy, they were transformed into nano-inclusion complexes (NICs). In this research, NICs were synthesized using ethanol as a reducing agent in the nanoprecipitation process. By employing FT-IR analysis, it was observed that hydrogen bonds were formed between QRC and ß-CD. Moreover, the IC molecules formed NICs through the aggregation facilitated by intermolecular hydrogen bonds. Proton NMR results further confirmed the occurrence of proton shielding and deshielding subsequent to the formation of NICs. The introduction of ß-CDs led to the development of a distinctive feather-like structure within the NICs. The particle sizes were consistently measured around 200 nm, and both SAED and XRD patterns indicated the absence of crystalline NICs, providing supporting evidence. Through cytotoxicity and fluorescence-assisted cell-sorting analysis, the synthesized NICs showed no significant damage in the cell line of MCF-7. In comparison to QRC alone, the presence of high concentrations of NICs exhibited a lesser degree of toxicity in normal human lung fibroblast MRC-5 cells. Moreover, the individual and combined administration of both low and high concentrations of NICs effectively suppressed the growth of cancer cells (MDA-MB-231). The solubility improvement resulting from the formation of QRC-NICs with ß-CD enhanced the percentage of cell survival for MCF-7 cell types.

Encapsulation of tannic acid in polyvinylidene fluoride mediated electrospun nanofibers and its antibiofilm and antibacterial activities.

In the past 15 years or more, interest in polymer-mediated nanofibers (NFs), a significant class of nanomaterials, has grown. Although fibers with a diameter of less than 1 mm are frequently commonly referred to as NFs, and are typically defined as having a diameter of less than several hundreds of nanometers. Due to the increased antibiotic resistance of many diseases nowadays, NFs with antibacterial activity are quite important. A flexible technique for creating NFs with the desired characteristics is called electrospinning. This research article describes how to make electrospun NFs of tannic acid (TA) with polyvinylidene fluoride (PVDF) as the template. As a result, the absorbance of the obtained NFs has been raised without forming any additional peaks in the spectral ranges. The obtained NF has a gradual increase in intensity, and the FT-IR data show that the TA is present in the NFs. FE-SEM images show that the NFs are discovered to be completely bead-free. Since TA reduced the viscosity of the spinning solution while marginally increasing solution conductivity, PVDF NFs have a greater average fiber diameter (AFD) than NFs of TA with PVDF, which is likely a result of the TA solutions in it. The findings showed that TA greatly decreased S. aureus and E. coli's ability to attach. The acquired NFs created in this work may have significant potential for reducing the pathogenicity of S. aureus and E. coli as well as their ability to build biofilms.

A novel and water-soluble material for coronavirus inactivation from oseltamivir in the cavity of methyl and sulfated-ß-cyclodextrins through inclusion complexation.

A potentially active water-soluble anti-viral with lesser toxic material from the Oseltamivir (OTV) has been produced by the sonication method. The formed material has been further characterized by UV-visible, FT-IR, powder XRD, SEM, TGA/DTA, ROESY, XPS, AFM and etc., The results of DFT calculation have proven that inclusion complexes (ICs) are theoretically and energetically more advantageous models and structures have also been proposed based on the results. Analysis of drug release has been carried out at three pH levels, and it is revealed the analysis is most helpful at acidic pH levels for the ICs with S-CD over H-CD. Over OTV without CDs, OTV:S-CD-ICs exhibited a very less cytotoxic ability on cancer cell lines than ICs with M-CD. ICs enhanced the coronavirus inactivation nature of OTV. This study provides for the first time a full characterization of ICs of OTV with CDs and highlights the impact of complexation on pharmacological activity.