Assuntos
Biologia Celular/normas , Detecção Precoce de Câncer/normas , Laboratórios/normas , Programas de Rastreamento/normas , Guias de Prática Clínica como Assunto , Neoplasias do Colo do Útero/diagnóstico , Alphapapillomavirus/isolamento & purificação , Automação Laboratorial/normas , Biologia Celular/organização & administração , Colo do Útero/patologia , Colo do Útero/virologia , Colposcopia/métodos , Feminino , Humanos , Armazenamento e Recuperação da Informação/legislação & jurisprudência , Armazenamento e Recuperação da Informação/normas , Laboratórios/organização & administração , Auditoria Médica , Pessoal de Laboratório Médico/educação , Pessoal de Laboratório Médico/organização & administração , Pessoal de Laboratório Médico/normas , Manejo de Espécimes/normas , Reino Unido , Neoplasias do Colo do Útero/virologia , Recursos HumanosRESUMO
Immunoglobulin heavy chain (IgH) gene rearrangement analysis was performed on 27 fine needle aspiration (FNA) specimens (13 reactive hyperplasia, 11 B cell non-Hodgkin's lymphoma (B-NHL), one Hodgkin's disease and two suspicious of non-Hodgkin's lymphoma). Satisfactory amplification was achieved in 23/27 cases. A polyclonal pattern was seen in 14 cases (11 reactive hyperplasia, one B-NHL, one suspicious of lymphoma, one Hodgkin's disease). A monoclonal band was seen in nine cases (eight B-NHL, one reactive hyperplasia). Amplification was unsuccessful in four cases. Clonal analysis by PCR-based IgH gene rearrangement analysis can be successfully applied to FNA material and can be useful in diagnosis, but the results must be interpreted in conjunction with morphology and other ancillary information.
Assuntos
Clonagem Molecular , Rearranjo Gênico de Cadeia Pesada de Linfócito B , Reação em Cadeia da Polimerase , Biópsia por Agulha/métodos , Doença de Hodgkin/genética , Doença de Hodgkin/patologia , Humanos , Linfoma de Células B/genética , Linfoma de Células B/patologia , Pseudolinfoma/genética , Pseudolinfoma/patologiaRESUMO
A case of cervical adenocarcinoma arising in diffuse mesonephric hyperplasia is presented. The hyperplastic and neoplastic tubules showed focal endocrine cell differentiation. Endocrine cells stained with the Grimelius technique, and were immunoreactive for chromogranin and serotonin. Antisera to additional specific hormones were negative. The natural history of this rare tumor is uncertain; the patient presented in this report is free of disease at 10-year follow-up.
Assuntos
Mesonefroma/patologia , Neoplasias do Colo do Útero/patologia , Adulto , Diferenciação Celular , Colo do Útero/patologia , Cromograninas/análise , Feminino , Humanos , Histerectomia , Técnicas Imunoenzimáticas , Queratinas/análise , Mesonefroma/cirurgia , Serotonina/análise , Coloração e Rotulagem , Neoplasias do Colo do Útero/cirurgia , Útero/patologia , Vimentina/análiseRESUMO
The cervical cytology and histology specimens from 200 patients referred to colposcopy with borderline nuclear abnormality were reviewed. Human papillomavirus (HPV)-associated changes were identified in 103 of 200 (53%) referral smears and in 139 of 150 (91%) biopsy specimens. Cervical intraepithelial neoplasia (CIN) was less frequently diagnosed on review compared with the original histopathology reports (30.7% vs 45.4%); the discrepancy was largely attributable to a lower incidence of CINI. There was agreement in the grading of borderline nuclear abnormality in 161 of 200 referral smears following review. Twenty-three smears were upgraded to mild dyskaryosis, whereas 16 were reclassified as negative.
Assuntos
Colo do Útero/patologia , Esfregaço Vaginal , Feminino , Humanos , Papillomaviridae , Infecções por Papillomavirus/diagnóstico , Infecções por Papillomavirus/patologia , Infecções Tumorais por Vírus/diagnóstico , Infecções Tumorais por Vírus/patologia , Doenças do Colo do Útero/diagnóstico , Doenças do Colo do Útero/patologia , Neoplasias do Colo do Útero/diagnóstico , Neoplasias do Colo do Útero/patologia , Displasia do Colo do Útero/diagnóstico , Displasia do Colo do Útero/patologiaRESUMO
The cytological and histological findings of a case of breast carcinoma with osteoclast-like giant cells are presented. A fine needle aspiration specimen demonstrated the characteristic combination of malignant epithelium and reactive multinucleated cells and enabled identification of this rare variant of breast cancer. Immunocytochemical studies using the monoclonal antibody KP1[CD68] support a histiocytic origin for the osteoclast-like cells.
Assuntos
Neoplasias da Mama/patologia , Células Gigantes , Osteoclastos/patologia , Idoso , Idoso de 80 Anos ou mais , Biópsia por Agulha , Feminino , Humanos , Imuno-HistoquímicaRESUMO
It has been suggested that the coagulation defect in haemophilia A protects these patients from coronary artery disease. We report two patients with severe haemophilia, who each had severe atheroma localised to the coronary arteries. Both patients had received only small amounts of factor VIII concentrates in their lifetime because of various social reasons. In one case death resulted from myocardial infarction secondary to an occlusive thrombus in a coronary artery. The implications of these findings to the pathogenesis of vascular disease are discussed.