RESUMO
Six anthracycline antitumor agents, marcellomycin, musettamycin, rudolphomycin, alcindoromycin, collinemycin, and mimimycin, have now been isolated from bohemic acid complex. This has been achieved by classical column chromatography with Sephadex LH-20 and ammonia-neutralized silica and by analytical and prepatative hplc techniques with normal phase systems containing aqueous ammonia.
Assuntos
Antibacterianos/análise , Antibióticos Antineoplásicos/isolamento & purificação , Naftacenos/análise , Naftacenos/isolamento & purificação , Animais , Antraciclinas , Antibióticos Antineoplásicos/farmacologia , Cromatografia em Gel , Cromatografia Líquida de Alta Pressão , Feminino , Leucemia L1210/tratamento farmacológico , Masculino , Melanoma/tratamento farmacológico , Camundongos , Testes de Sensibilidade Microbiana , Naftacenos/farmacologia , Neoplasias Experimentais/tratamento farmacológico , Alcaloides de Pirrolizidina/isolamento & purificação , Alcaloides de Pirrolizidina/farmacologiaRESUMO
In a screening program for antitumor substances from microbial fermentations, a compound was isolated from the mycelium of an Aspergillus species which proved to be the mycotoxin 5-methoxysterigmatocystin. This and the subsequently isolated sterigmatocystin gave significant inhibition of the transplanted mouse leukemias P-388 and L-1210. Preparation and testing of several derivatives of 5-methoxysterigmatocystin suggest that certain functions in the molecule are essential for tumor inhibition. Although slight antitumor effects have been reported for the chemically related aflatoxins, we believe this is the first report of antitumor activity of sterigmatocystins.
