RESUMO
We analyze geographic genetic variation in C. ocellatus to evaluate the influences of major climatic, paleogeographic and anthropogenic factors in its biogeographic history. Ninety four specimens from 61 populations were collected across all of its geographical range and analyzed based on partial mitochondrial sequences (cyt b, 12S, and ND1). Our results demonstrate that an ancestral form of C. ocellatus, which expanded in northwestern Africa at the end of Miocene, diverged in at least three separate evolutionary lineages approximately 4.57Ma: C. humilis spread south of the Sahara, while the other two (C. ocellatus sensu stricto) were restricted in the coastal North African region. The complicated history of the ocellated skink is a result of multiple vicariant phenomena followed by multiple active or passive dispersals. The Messinian salinity crisis and the re-flooding of the Mediterranean basin, the climatic transition from Middle to Upper Pliocene, and the hyperarid phase of the Sahara, affected the distribution and diversification of C. ocellatus, while in historical times it was introduced in the central Mediterranean islands and eastern Mediterranean region from Tunisia and Cyrenaica, respectively.
Assuntos
Evolução Molecular , Genética Populacional , Lagartos/genética , Filogenia , Animais , Teorema de Bayes , DNA Mitocondrial/genética , Geografia , Funções Verossimilhança , Lagartos/classificação , Região do Mediterrâneo , Modelos Genéticos , Dinâmica Populacional , Alinhamento de Sequência , Análise de Sequência de DNARESUMO
The snake-eyed lizards of the genus Ophisops (Lacertidae) have been through a series of taxonomical revisions, but still their phylogenetic relationships remain uncertain. In the present study we estimate the phylogeographic structure of O. elegans across its distributional range and we evaluate the relationships between O. elegans and the sympatric, in North Africa, species O. occidentalis, using partial mtDNA sequences (16S rRNA, COI, and cyt b). All phylogenetic analyses produced topologically identical trees where extant populations of O. elegans and O. occidentalis were found polyphyletic. Taking into account all the potential causes of polyphyly (introgressive hybridization, incomplete lineage sorting, and imperfect taxonomy) we suggest the inaccurate taxonomy as the most likely explanation for the observed pattern. Our results stress the need for re-evaluation of the current taxonomical status of these species and their subspecies. Furthermore, our biogeographic analyses and the estimated time of divergences suggest a late Miocene diversification within these species, where the present distribution of O. elegans and O. occidentalis was the result of several dispersal and vicariant events, which are associated with climatic oscillations (the late Miocene aridification of Asia and northern Africa) and paleogeographic barriers of late Miocene and Pliocene period.
Assuntos
DNA Mitocondrial/genética , Evolução Molecular , Lagartos/genética , Filogenia , Animais , Teorema de Bayes , Citocromos b/genética , Complexo IV da Cadeia de Transporte de Elétrons/genética , Genes Mitocondriais , Genes de RNAr , Especiação Genética , Geografia , Funções Verossimilhança , Lagartos/classificação , Mitocôndrias/genética , Modelos Genéticos , RNA Ribossômico 16S , Alinhamento de Sequência , Análise de Sequência de DNA , Estatísticas não ParamétricasRESUMO
Mesalina is a widespread lacertid genus occurring throughout the Saharo-Sindian region from North Africa to Pakistan. It has been through a series of taxonomic revisions, but the phylogenetic relationships among the species remain unclear. In this study we estimate the phylogeographic structure of M. guttulata across most of its distributional range and we evaluate the relationships between M. guttulata and the sympatric species M. brevirostris and M. bahaeldini using partial mitochondrial DNA (mtDNA) sequences (cyt b and 16S). M. guttulata and M. brevirostris represent species complexes, whereas M. bahaeldini considered before as M. guttulata is a recently described species with very restricted distribution. Here we present the first evidence that M. guttulata is a paraphyletic taxon with respect to M. bahaeldini, while M. brevirostris proves to be a polytypic species or even a species complex, confirming previous studies. Although mtDNA markers have several properties that make them suitable for phylogeographic studies, they are not free of difficulties. Phylogeographic inferences within and between closely related species can be mislead by introgression and retention of ancestral polymorphism (incomplete lineage sorting). However, the present distribution pattern, the estimated times of divergence and the significant variation in morphology within M. guttulata led us to accept that the paraphyletic pattern observed, is most likely due to inaccurate taxonomy. Our hypothesis is that what has hitherto been considered as intraspecific variation, actually reflects species-level variation. Furthermore, our biogeographic analyses and the estimated time of divergences suggest that the present distribution of M. guttulata was the result of several dispersal and vicariant events, which are associated with historical changes (climatic oscillations and paleogeographic barriers) of late Miocene and Pliocene period.
Assuntos
Lagartos/classificação , Lagartos/genética , Filogenia , Animais , Teorema de Bayes , Citocromos b/genética , DNA Mitocondrial/genética , Evolução Molecular , Genes Mitocondriais , Genes de RNAr , Geografia , Funções Verossimilhança , Mitocôndrias/genética , Modelos Genéticos , RNA Ribossômico 16S/genética , Alinhamento de Sequência , Análise de Sequência de DNA , Especificidade da EspécieRESUMO
Sequence data derived from three mitochondrial markers (cytochrome b, 16S rRNA and 12S rRNA genes) were used to infer the evolutionary history of several insular and mainland populations of the Greek legless skink (Ophiomorus punctatissimus), covering most of its distributional range. All phylogenetic analyses produced topologically identical trees that revealed a well-resolved phylogeny. These trees support two O. punctatissimus clades, which are geographically separated (west and east of the mid-Aegean trench). The assumption of a clock-like evolution could not be rejected, and thus a local clock was calibrated for the O. punctatissimus lineages. The non-overlapping geographic distributions of the major clades suggest a spatial and temporal sequence of diversification that coincides with paleogeographic separations during the geological history of the Aegean region. It seems that O. punctatissimus is an old eastern Mediterranean species that has been differentiating in this region at least from middle Miocene. It is possible that the ancestral form of O. punctatissimus invaded the Aegean region from Anatolia before the complete formation of the mid-Aegean trench, when the Aegean was still a uniform landmass, while other vicariant events have led to its present distribution.
Assuntos
Lagartos/classificação , Filogenia , Animais , Variação Genética , Geografia , Lagartos/genéticaRESUMO
Phylogenetic relationships in the western fraction of Rana (Pelophylax) have not been resolved till now, even though several works have been devoted to the subject. Here, we infer phylogenetic relationships among the species distributed in the area of the Eastern Mediterranean, comparing partial mitochondrial DNA sequences for the cytochrome b and 16S rRNA genes. The obtained molecular data clearly indicate that Western Palearctic water frogs underwent a basal radiation into at least 3 major lineages (the perezi, the lessonae, and the rindibunda/bedriagae lineages) advocating an upper Miocene speciation. Moreover, we consider that within the rindibunda/bedriagae lineage, R. (P.) ridibunda, R. (P.) epeirotica, R. (P.) cretensis, R. (P.) bedriagae, R. (P.) cerigensis and R. (P.) kurtmuelleri were differentiated from a common ancestor through a series of vicariant and dispersal events, during the last approximately 5Mya, even though the specific rank of some taxa may be questionable, such as R. cerigensis in respect with R. bedriagae and R. kurtmuelleri in respect with R. ridibunda.
Assuntos
DNA Mitocondrial/genética , Filogenia , Ranidae/genética , Animais , Grupo dos Citocromos b/genética , Geografia , Região do Mediterrâneo , Dados de Sequência Molecular , RNA Ribossômico 16S/genética , Ranidae/classificação , Análise de Sequência de DNARESUMO
Sequence data derived from two mitochondrial markers, 16S rRNA and COI genes, were used to infer the evolutionary history of 47 insular and mainland populations covering most of the distributional range of the northeastern Mediterranean scorpion species Mesobuthus gibbosus. Based on the estimated divergence times of Mesobuthus lineages, the temporal frame of the genus differentiation in the northeastern Mediterranean region is placed in middle Miocene (15 million years ago). The biogeographic affinities of M. gibbosus populations point towards a mainly vicariant pattern of differentiation of the species which is consistent with the geological events that transformed the Aegean region during the period from 12 to 5 million years ago. M. gibbosus is an old northeastern Mediterranean species that has retained valuable bits of genetic information, reflecting some of the oldest vicariant events that have occurred in the area. Most importantly, the history witnessed by M. gibbosus has not been obscured by more recent palaeoevents of the region. Therefore, the case of M. gibbosus is in favour of a taxon-oriented 'perception' of the natural history of a given area.
Assuntos
Evolução Molecular , Escorpiões/genética , Animais , Teorema de Bayes , Geografia , Grécia , Região do Mediterrâneo , Filogenia , Dinâmica Populacional , Fatores de TempoRESUMO
Examination of the association between reproductive isolation and genetic divergence in a variety of organisms is essential for elucidating the mechanisms causing speciation. However, such studies are lacking for hermaphrodites. We measured premating (sexual) isolation in species pairs of the hermaphroditic land snail Albinaria and we compared it with their genetic divergence. We did not find substantial sexual isolation barriers between the species studied. The absence of strong sexual isolation between species implies its minor effect in the evolution of this genus, because distributional, population and life-history characteristics of Albinaria make mate-choice possibly redundant. Furthermore, we found disassociation between genetic divergence and sexual isolation, suggesting that they do not form necessarily a cause-effect duet. However, Albinaria voithii, the only dextral Albinaria species, shows strong sexual isolation against the other sinistral species. We discuss whether change in coiling either has triggered instantaneous speciation, or is an example of character displacement.
Assuntos
Especiação Genética , Comportamento Sexual Animal , Caramujos/genética , Animais , DNA Mitocondrial/análise , Transtornos do Desenvolvimento Sexual , Filogenia , Dinâmica Populacional , Caramujos/anatomia & histologia , Caramujos/fisiologiaRESUMO
Wall lizards of the genus Podarcis (Sauria, Lacertidae) comprise 17 currently recognized species in southern Europe, where they are the predominant nonavian reptile group. The taxonomy of Podarcis is complex and unstable. Based on DNA sequence data, the species of Podarcis falls into four main groups that have substantial geographic coherence (Western island group, southwestern group, Italian group, and Balkan Peninsula group). The Balkan Peninsula species are divided into two subgroups: the subgroup of P. taurica (P. taurica, P. milensis, P. gaigeae, and perhaps P. melisellensis), and the subgroup of P. erhardii (P. erhardii and P. peloponnesiaca). In the present study, the question of phylogenetic relationships among the species of Podarcis encountered in the Balkan Peninsula was addressed using partial mtDNA sequences for cytochrome b (cyt b) and 16S rRNA (16S). The data support the monophyly of Podarcis and suggest that there are three phylogenetic clades: the clade A (P. taurica, P. gaigeae, P. milensis, and P. melisellensis); the clade B (P. erhardii and P. peloponnesiaca), and the clade C (P. muralis and P. sicula). By examining intraspecific relationships it was found that extant populations of P. erhardii are paraphyletic. Furthermore, subspecies previously defined on the basis of morphological characteristics do not correspond to different molecular phylogenetic clades, suggesting that their status should be reconsidered. The distinct geographic distribution of the major clades of the phylogenetic tree and its topology suggest a spatial and temporal sequence of phylogenetic separations that coincide with some major paleogeographic separations during the geological history of the Aegean Sea. The results stress the need for a reconsideration of the evolutionary history of Balkan Podarcis species and help overcome difficulties that classical taxonomy has encountered at both the species and subspecies level.
Assuntos
Demografia , Evolução Molecular , Lagartos/genética , Filogenia , Albânia , Animais , Sequência de Bases , Teorema de Bayes , DNA Mitocondrial/genética , Geografia , Grécia , Funções Verossimilhança , Modelos Genéticos , Dados de Sequência Molecular , República da Macedônia do Norte , Análise de Sequência de DNARESUMO
Wall lizards of the genus Podarcis (Sauria, Lacertidae) comprise 17 currently recognized species in southern Europe, where they are the predominant reptile group. The taxonomy of Podarcis is complex and unstable. Based on DNA sequence data the species of Podarcis falls into four main groups that have substantial geographical conherence (western island group, southwestern group, Italian group and Balkan group). The Balkan species are divided in two subgroups: the subgroup of Podarcis taurica (P. taurica, P. milensis, P. gaigeae and perhaps P. melisellensis), and the subgroup of Podarcis erhardii (P. erhardii and P. peloponnesiaca). We addressed the question of phylogenetic relations among the species of the P. taurica subgroup encountered in Greece, as they can be inferred from partial mtDNA (cyt b and 16S) sequences. Our data support the monophyly of P. taurica subgroup and suggest that P. gaigeae, P. milensis and P. melisellensis form a clade, which thereinafter connects to P. taurica. Within the previous clade, P. gaigeae is more closely related to P. milensis than to P. melisellensis. However, the specimens of P. taurica were subdivided in two different groups. The first one includes the specimens from northeastern Greece, and the other group includes the specimens from the rest of continental Greece and Ionian islands. Because the molecular clock of the cyt b and 16 rRNA genes was not rejected in our model test, it is possible to estimate times of speciation events. Based on the splitting of the island of Crete from Peloponnisos [c. 5 million years ago (Ma)], the evolutionary rate for the cyt b is 1.55% per million years (Myr) and for the 16S rRNA is 0.46% per Myr. These results suggest that the evolutionary history of P. taurica in Greece is more complex than a single evolutionary invasion. The data analysed, stress the need for a reconsideration of the evolutionary history of Greek Podarcis species and help overcome difficulties that classical taxonomy has encountered at both the species level.
Assuntos
Demografia , Evolução Molecular , Lagartos/genética , Filogenia , Animais , Sequência de Bases , Teorema de Bayes , DNA Mitocondrial/genética , Geografia , Grécia , Funções Verossimilhança , Modelos Genéticos , Dados de Sequência Molecular , Dinâmica Populacional , Análise de Sequência de DNARESUMO
Cyrtopodion kotschyi is a small gecko, widely distributed on the islands of the Aegean archipelago and the adjacent mainland. We unveiled the phylogeography of the species by using a portion of the cytochrome oxidase I mitochondrial DNA gene from 35 insular and mainland populations. The distinct geographic distribution of the major clades of the phylogenetic tree and its topology suggest a spatial and temporal sequence of phylogenetic separations that coincide with some major paleogeographical separations during the geological history of the Aegean and support a mainly vicariant pattern of differentiation. The separation times and 95% confidence limits among the different clades were estimated according to two different paleogeographical scenarios. The very high interpopulation genetic divergence (up to 20% uncorrected pairwise distances) and the better concordance between paleogeographical and phylogenetic separations for one of the scenarios suggest that species differentiation started during Miocene (about 10 Mya) due to the fragmentation of the united landmass of "Agais" that was Aegean at that time.
Assuntos
Demografia , Evolução Molecular , Lagartos/genética , Filogenia , Animais , Sequência de Bases , Teorema de Bayes , Primers do DNA , Complexo IV da Cadeia de Transporte de Elétrons/genética , Geografia , Fenômenos Geológicos , Geologia , Grécia , Funções Verossimilhança , Modelos Genéticos , Dados de Sequência Molecular , Análise de Sequência de DNARESUMO
Sequence data derived from two mitochondrial markers, 16S rRNA and cytochrome b genes, were used to infer the phylogenetic relationships of 38 populations of the snake-eyed skinks of the genus Ablepharus with emphasis on A. kitaibelii from Greece and Turkey. The partition-homogeneity tests indicated that the combined data set was homogeneous, and maximum-parsimony, maximum-likelihood, and Bayesian analyses produced topologically identical trees that revealed a well-resolved phylogeny. All species except A. kitaibelii form monophyletic units. The latter species appears paraphyletic with respect to A. budaki and A. chernovi with populations clustering into two distinct clades. A. chernovi and A. budaki, which have recently been raised to species status, were confirmed as genetically distinct forms. We used sequence divergence and paleogeographic history of the Aegean region to reconstruct a biogeographic evolutionary scenario for A. kitaibelii.
Assuntos
Evolução Molecular , Lagartos/genética , Filogenia , Animais , Teorema de Bayes , Citocromos b/genética , Variação Genética , Geografia , Funções Verossimilhança , RNA Ribossômico 16S/genéticaRESUMO
The coordination properties of the peptide Ac-GluLeuAlaLysHisAla-amide, the C-terminal 102-107 fragment of histone H2B towards Cu(II) and Ni(II) ions were studied by means of potentiometry and spectroscopic techniques (UV/Vis, CD, EPR and NMR). It was found that the peptide has a unique ability to bind Cu(II) ions at physiological pH values at a Cu(II): peptide molar ratio 1:2, which is really surprising for blocked hexapeptides containing one His residue above position 3. At physiological pH values the studied hexapeptide forms a CuL(2) complex {N(Im),2N(-)}, while in acidic and basic pH values the equimolar mode is preferred. In basic solutions Ac-GluLeuAlaLysHisAla-amide may bound through a {4N(-)} mode forming a square-planar complex, in which the imidazole ring is not any more coordinated or it has been removed in an axial position. On the contrary, Ni(II) ions form only equimolar complexes, starting from a distorted octahedral complex at about neutral pH values to a planar complex, where hexapeptide is bound through a {N(Im),3N(-)} mode in equatorial plane. The results may be of importance in order to reveal more information about the toxicity caused by metals and furthermore their influence to the physiologic metabolism of the cell.
Assuntos
Cobre/química , Histonas/química , Níquel/química , Sequência de Aminoácidos , Sítios de Ligação , Dicroísmo Circular , Espectroscopia de Ressonância de Spin Eletrônica , Técnicas In Vitro , Espectroscopia de Ressonância Magnética , Estrutura Molecular , Oligopeptídeos/síntese química , Oligopeptídeos/química , Fragmentos de Peptídeos/síntese química , Fragmentos de Peptídeos/química , Prótons , EspectrofotometriaRESUMO
Erhard's wall lizard, Podarcis erhardii (Sauria: Lacertidae), is highly diversified in Greece and especially in the southern Aegean region. Out of the 28 recognized subspecies, 27 are found in Greece from the North Sporades island-complex in the North Aegean (grossly south of the 39th parallel) to the island of Crete in the South. The species exhibits great morphological and ecological plasticity and inhabits many different habitats from rocky islets and sandy shores to mountaintops as high as 2000m. By examining intraspecific variability at a segment of the mitochondrial gene cytochrome b we have found that that extant populations of P. erhardii are paraphyletic. Furthermore, we have found that subspecies previously defined on the basis of morphological characteristics do not correspond to different molecular phylogenetic clades, so that their status should be reconsidered. The DNA based biogeographical and phylogenetic history of Podarcis in Southern Greece is congruent with available paleogeographic data of the region, which supports the view that DNA sequences may be a useful tool for the study of palaeogeography.
Assuntos
Lagartos/classificação , Filogenia , Animais , Sequência de Bases , Evolução Biológica , Grupo dos Citocromos b/genética , Geografia , Grécia , Funções Verossimilhança , Lagartos/genética , Dados de Sequência Molecular , PaleontologiaRESUMO
CH(3)CO-Thr-Glu-Ser-His-His-Lys-NH(2), a hexapeptide representing the 120-125 sequence of histone H2A, coordinates Cu(II) ions efficiently. Monomeric complexes are formed. In the major complex at physiological pH, CuH(-1)L, Cu(II) is coordinated equatorially through the imidazole nitrogen of the His-4 residue and the amide nitrogens of the Ser-3 and His-4 residues, and axially through the imidazole nitrogen of the His-5 residue. This complex reacts with H(2)O(2) and the resulting reactive oxygen intermediate efficiently oxidizes 2'-deoxyguanosine. The underlying mechanism involves the formation of Cu(III) and a metal-bound hydroxyl radical species.