Micro-computed tomography evaluation of general trends in aligner thickness and gap width after thermoforming procedures involving six commercial clear aligners: An in vitro study.

OBJECTIVE: To assess the effects of thermoforming on aligner thickness and gap width in six aligner systems with the same nominal thickness. METHODS: Six passive upper aligners of different brands were adapted to a single printed cast. Each sample was evaluated with high-resolution micro-computed tomography. To investigate aligner thickness and gap width, two-dimensional (2D) analysis was conducted assessing the effects of the following variables: tooth type (central incisor, canine, and first molar), 2D reference points, and aligner type. Data were analyzed and compared using analysis of variance and Tukey's post-hoc tests (p < 0.05). RESULTS: Tooth type, dental region, and aligner type affected both the gap width and aligner thickness. The aligner thickness remained moderately stable across the arch only in the F22. CONCLUSIONS: All thermoformed samples displayed smaller aligner thickness and gap width at anterior teeth and both gingival and coronal centers than at posterior teeth and occlusal surfaces.

MicroCT X-ray comparison of aligner gap and thickness of six brands of aligners: an in-vitro study.

BACKGROUND: To investigate and compare the gap (i.e. fit) and thickness of six aligner systems (Airnivol, ALL IN, Arc Angel, F22, Invisalign and Nuvola) using industrial computed tomography (CT). The null hypothesis was that there would be no detectable differences in either measurement between the aligners investigated. MATERIALS AND METHODS: Passive aligners of each brand were fitted to one single resin cast prototyped from an STL file from a single patient. The samples obtained were examined under high-resolution micro-CT, and the resulting tomographic microphotographs and volumetric data were compared. 3D analysis investigated the gap volume, the mean gap width and the maximum gap width of each sample. A total of 204 linear 2D measurements were made on 18 microtomographic images to investigate the aligner gap and thickness among different systems. Investigated regions were the central incisor, canine and first molar. The resulting measurements were analysed by ANOVA and compared using Tukey's post hoc analysis (P < 0.05). RESULTS: 3D analysis revealed that the F22 displayed lower gap volume and mean gap width, followed by Airnivol and Invisalign, whereas Airnivol the lowest maximum gap width. 2D analysis showed that F22 had the lowest mean gap and aligner thickness at all teeth investigated. Comparison of the 2D point values revealed statistically significant differences between brands in terms of both measurements (P < 0.05), with the exception of six points in the gap analysis and one in the thickness analysis. CONCLUSIONS: There are differences between the six aligner systems examined in terms of 2D and 3D measurements of aligner thickness and gap.

Scanning Small- and Wide-Angle X-ray Scattering Microscopy Selectively Probes HA Content in Gelatin/Hydroxyapatite Scaffolds for Osteochondral Defect Repair.

This study is aimed at investigating the structure of a scaffold made of bovine gelatin and hydroxyapatite for bone tissue engineering purposes. In particular, the detailed characterization of such a material has a great relevance because of its application in the healing process of the osteochondral defect that consists of a damage of cartilage and injury of the adjacent subchondral bone, significantly compromising millions of patient's quality of life. Two different techniques exploiting X-ray radiation, with table-top setups, are used: microtomography (micro-CT) and microdiffraction. Micro-CT characterizes the microstructure in the three dimensions at the micrometer scale spatial resolution, whereas microdiffraction provides combined structural/morphological information at the atomic and nanoscale, in two dimensional microscopy images with a hundred micrometer spatial resolution. The combination of these two techniques allowed an appropriate structural characterization for the purpose of validating the engineering approach used for the realization of the hydroxyapatite gradient across the scaffold, with properties close to the natural model.

The determination of the efficiency of energy dispersive X-ray spectrometers by a new reference material.

A calibration procedure for the detection efficiency of energy dispersive X-ray spectrometers (EDS) used in combination with scanning electron microscopy (SEM) for standardless electron probe microanalysis (EPMA) is presented. The procedure is based on the comparison of X-ray spectra from a reference material (RM) measured with the EDS to be calibrated and a reference EDS. The RM is certified by the line intensities in the X-ray spectrum recorded with a reference EDS and by its composition. The calibration of the reference EDS is performed using synchrotron radiation at the radiometry laboratory of the Physikalisch-Technische Bundesanstalt. Measurement of RM spectra and comparison of the specified line intensities enables a rapid efficiency calibration on most SEMs. The article reports on studies to prepare such a RM and on EDS calibration and proposes a methodology that could be implemented in current spectrometer software to enable the calibration with a minimum of operator assistance.