Evaluation of biological selenium nanoparticles on growth performance, histopathology of vital organs and genotoxicity in Japanese quails (coturnix coturnix japonica).

Research on the effects of selenium nanoparticles (Se-NPs), particularly in Japanese quails, is lacking, especially regarding the potential for DNA damage. Therefore, this study aimed to investigate the impact of administering 0.2 and 0.4 mg/kg of Se-NPs on the growth performance, DNA integrity, and histopathological alterations of the liver, lung, kidney, and heart in quails. A total of 480 one-day-old Japanese quails were divided into three experimental groups as follows: Group 1 served as the control and received only basic feed, while Group 2 and 3 received 0.2 mg/kg and 0.4 mg/kg of Se-NPs via oral gavage. Our results suggested that, birds fed with Se-NPs at both levels significantly (p < .01) reduced feed intake, however, weight gain was significantly (p < .01) increased in quails supplemented with 0.2 mg/kg. Similarly, feed conversion ratio (FCR) was significantly (p < .01) reduced in group supplemented with 0.2 mg/kg Se-NPs. White blood cells increased significantly (P0.01) in 0.4 mg/kg while haemoglobin and red cell distribution width decreased (p < .01) in the same group. Both treatment regimens resulted in DNA damage and histopathological alterations; however, the adverse effects were more prominent in the group receiving the higher dose of 0.4 mg/kg. These findings indicate that the lower dose of 0.2 mg/kg may have beneficial effects on growth. However, the higher dose of 0.4 mg/kg not only negatively impacts growth but also leads to histopathological alterations in major organs of the body and DNA damage as well.

Impact of Postoperative Antibiotic Prophylaxis on Surgical Site Infections Rates After Mastectomy with Drains but Without Immediate Reconstruction: A Multicenter, Double-Blinded, Randomized Control Superiority Trial.

BACKGROUND: There is no consensus on the use of postoperative antibiotic prophylaxis (PAP) after mastectomy with indwelling drains. We explored the utility of continued PAP in reducing surgical site infection (SSI) rates after mastectomy without immediate reconstruction and with indwelling drains. PATIENTS AND METHODS: A multicenter, two-armed, randomized control superiority trial was conducted in Pakistan. We enrolled all consenting adult patients undergoing mastectomy without immediate reconstruction. All patients received a single preoperative dose of cephalexin within 60 min of incision, and postoperatively were randomized to receive either continued PAP using cephalexin (intervention) or a placebo (control) for the duration of indwelling, closed-suction drains. The primary outcome was the development of SSI within 30 days and 90 days postoperatively. Secondary outcomes included study-drug-associated adverse events. Intention-to-treat analysis was performed using multivariable Cox regression. RESULTS: A total of 369 patients, 180 (48.8%) in the intervention group and 189 (51.2%) in the control group, were included in the final analysis. Overall cumulative SSI rates were 3.5% at 30 days and 4.6% at 90 days postoperatively. PAP was not associated with SSI reduction at 30 (hazard ratio, HR 1.666 [95% confidence interval CI 0.515-5.385]) or 90 (1.575 [0.558-4.448]) days postoperatively, or with study-drug-associated adverse effects (0.529 [0.196-1.428]). CONCLUSIONS: Continuing antibiotic prophylaxis for the duration of indwelling drains after mastectomy without immediate reconstruction offers no additional benefit in terms of SSI reduction. There is a need to update existing guidelines to provide clearer recommendations regarding use of postoperative antibiotic prophylaxis after mastectomy in the setting of indwelling drains.