[Analysis of the kinetic growth patterns of cultured cells. III. The effect of inoculation density, of a geroprotector-antioxidant and of stationary-phase aging]. / Analiz nekotorykh kineticheskikh zakonomernostei rosta kul'tiviruemykh kletok. III. Vliianie plotnost poseva, geroprotektora-antioksidanta, "statsionarnogo stareniia".

With the help of a previously devised model of cultured cell growth kinetics it was shown that the "plateau" level on the growth curve of human embryo diploid fibroblasts increased, if the cells were grown with Epigid geroprotector-antioxidant (equal results with two drug concentrations: 10(-5) and 10(-7) M, resp.). It was also found that the "plateau" level on the growth curve of cultured "3 days aged" Chinese hamster cells (subcultivated to a fresh medium 3 days after a 1:6 subcultivation) lays higher than the "plateau" level of "14 days aged" cells but lower than that of "7 day aged" cells. Furthermore it was shown that an increase in inoculation density of Chinese hamster cells increased proportionately the rate of cell population growth but had a little effect on the "plateau" level of the growth curve. The data obtained are discussed in terms of some "proliferative" theories of cellular ageing.

[Analysis of the kinetic growth patterns of cultured cells. II. The action of ionizing radiation, an alkylating agent and a low-frequency electromagnetic field]. / Analiz nekotorykh kineticheskikh zakonomernostei rosta kul'tiviruemykh kletok. II. Deistvie ioniziruiushchei radiatsii, alkiliruiushchego agents, nizkochastotnogo élektromagnitnogo polia.

With the help of the earlier devised model of cultured cell growth kinetics it is shown that the "plateau" level on the growth curve of Chinese hamster cells decreased proportionally to a dose of the agent applied after a short-term treatment by gamma-radiation or by alkylating agent thiophosphamide. The analysis of our own and literature data enabled us to propose that the lowering of the growth curve may testify to the geropromoter character (i.e. manifesting in ageing promoting) of the investigated factor action. As the low-frequency electromagnetic field induces similar changes in the growth curve, it is related (together with both the above factors investigated) to geropromoters.

[Analysis of the kinetic growth patterns of cultured cells. I. The model]. / Analiz nekotorykh kineticheskikh zakonomernostei rosta kul'tiviruemykh kletok. I. Model'.

On the basis of the Verhulst-Pearl equation, a model of cultured cell growth kinetics is devised. Analysing both authors' and literature data, a possibility was shown to use the model for description of growth kinetics of different cultured cell types. The proposed method of analysis of cell growth kinetic characteristics also provides a rapid evaluation of the cell population doubling time and of the relative number of non-dividing cells in the population.

[DNA degradation in resting cultured Chinese hamster cells]. / Degradatsiia DNK v pokoiashchikhsia kul'tiviruemykh kletkakh kitaiskogo khomiachka.

A gradual accumulation of the alkali-labile sites in DNA of cultured Chinese hamster cells during "stationary phase ageing" was shown by ultracentrifugation in alkaline sucrose gradients. A possibility is discussed of using the stationary phase mammalian cell cultures as a model for studies of ageing mechanisms.