Clinical Investigation of Large Volume Subcutaneous Delivery up to 25 mL for Lean and Non-Lean Subjects.

PURPOSE: To evaluate the clinical feasibility and tolerability of large volume subcutaneous delivery at different injection depths for lean and non-lean subjects. METHODS: A single-center, randomized, subject-blinded, crossover study in 62 healthy subjects was conducted to evaluate delivery of a 10-cP solution containing hyaluronic acid. Subjects were separated into lean and non-lean cohort by SC thickness. A syringe pump was used to study the effect of different volumes (5, 12, 25 mL) of a viscous placebo solution and needle lengths (6, 9 and 12 mm) delivered at 0.5 mL/min. RESULTS: Across all treatments, injection sites were observed to have negligible leakage, ~34 kPa of back pressure, and VAS of mild pain with higher pain from needle insertion than during injection. While mild to moderate erythema was the most frequently reported ISR and edema was most prominent for 25 mL injections, all ISRs were resolved within 4 hours post injection. Subjects were unbothered by ISRs across all treatments and rated them as low distress scores (average 1.0-1.5 out of 6). CONCLUSION: SC injection of 25 mL is feasible and tolerable using a low-pain formulation for abdomen injection irrespective of subcutaneous thickness and injection depths at a delivery rate of 0.5 mL/min.

Association between Multimorbidity and Psychological Distress among Older Adults in India: The Moderating Role of Elder Abuse.

OBJECTIVES: In this study, we examine the association between multimorbidity and psychological distress and to what extent elder abuse mediates the association. METHODS: We analyzed cross-sectional nationally representative data from the "Building Knowledge Base on Population Ageing in India (BKPAI)." Multivariate logistic regression was used to understand the association between multimorbidity and psychological distress. We used Karlson-Holm- Breen (KHB) method to understand the role of elder abuse in mediating the association between multimorbidity and psychological distress. RESULTS: Older adults who ever experienced abuse (OR = 1.92 95%CI = 1.62, 2.26, p < .001) or in last one month (OR = 2.09 95%CI = 1.65, 2.64, p < .001) reported higher odds of psychological distress. Further, older adults with four or more chronic diseases are thrice more likely to report psychological distress (OR = 3.03 95%CI = 2.38, 3.82, p < .001). The results further suggest the mediating role of abuse on the association between multimorbidity and psychological distress. CONCLUSIONS: The results suggest the role of elder abuse on the association between multimorbidity and psychological distress among older population in India. CLINICAL IMPLICATIONS: Creating an environment to reduce the abuse among older adults who have multimorbidity will be essential to reducing the psychological distress among older adults in India.

Modulation of 3D Bioprintability in Polysaccharide Bioink by Bioglass Nanoparticles and Multiple Metal Ions for Tissue Engineering.

BACKGROUND: Bioglasses are used in applications related to bone rehabilitation and repair. The mechanical and bioactive properties of polysaccharides like alginate and agarose can be modulated or improved using bioglass nanoparticles. Further essential metal ions used as crosslinker have the potential to supplement cultured cells for better growth and proliferation. METHOD: In this study, the alginate bioink is modulated for fabrication of tissue engineering scaffolds by extrusion-based 3D bioprinting using agarose, bioglass nanoparticles and combination of essential trace elements such as iron, zinc, and copper. Homogeneous bioink was obtained by in situ mixing and bioprinting of its components with twin screw extruder (TSE) based 3D bioprinting, and then distribution of metal ions was induced through post-printing diffusion of metal ions in the printed scaffolds. The mechanical and 3d bioprinting properties, microscopic structure, biocompatibility of the crosslinked alginate/agarose hydrogels were analyzed for different concentrations of bioglass. The adipose derived mesenchymal stem cells (ADMSC) and osteoblast cells (MC3T3) were used to evaluate this hydrogel's biological performances. RESULTS: The porosity of hydrogels significantly improves with the incorporation of the bioglass. More bioglass concentration results in improved mechanical (compressive, dynamic, and cyclic) and 3D bioprinting properties. Cell growth and extracellular matrix are also enhanced with bioglass concentration. CONCLUSION: For bioprinting of the bioinks, the advanced TSE head was attached to 3D bioprinter and in situ fabrication of cell encapsulated scaffold was obtained with optimized composition considering minimal effects on cell damage. Fabricated bioinks demonstrate a biocompatible and noncytotoxic scaffold for culturing MC3T3 and ADMSC, while bioglass controls the cellular behaviors such as cell growth and extracellular matrix formation.

Geochemical fractionation, mobility of elements and environmental significance of surface sediments in a Tropical River, Borneo.

Miri River is a tropical river in Borneo that drains on flat terrain and urbanised area and debauches into the South China Sea. This paper documents the environmental status of this river, and provides an insight into the provenance using bulk chemistry of the sediments, and brings out the geochemical mobility, bioavailability, and potential toxicity of some critical elements based on BCR sequential extraction. The sediments are intense to moderately weathered and recycled products of Neogene sedimentary rocks. The hydrodynamic characteristics of the river favoured an upstream section dominated by fine sand, while the downstream sediments are medium silt. Based on the bulk geochemistry, the Miri River sediments are moderate to considerably contaminated by Cu, Mo, and As in the upstream and by Sb, As and Cu in the downstream. The potential ecological risk values are low except Cu and a significant biological impact is expected in downstream due to Cu, As, Zn and Cr. The mobility, bioavailability and Risk Assessment Code values for Zn and Mn are higher and thus may pose moderate to very high risk to aquatic organisms. Though a high bulk concentration of Cu is observed, the association of Cu with the bioavailable fraction is low.

3D bioprinting of complex tissue scaffolds with in situ homogeneously mixed alginate-chitosan-kaolin bioink using advanced portable biopen.

Control of in situ 3D bioprinting of hydrogel without toxic crosslinker is ideal for tissue regeneration by reinforcing and homogeneously distributing biocompatible reinforcing agent during fabrication of large area and complex tissue engineering scaffolds. In this study, homogeneous mixing, and simultaneous 3D bioprinting of a multicomponent bioink based on alginate (AL)-chitosan (CH), and kaolin was obtained by an advanced pen-type extruder to ensure structural and biological homogeneity during the large area tissue reconstruction. The static, dynamic and cyclic mechanical properties as well as in situ self-standing printability significantly improved with the kaolin concentration for AL-CH bioink-printed samples due to polymer-kaolin nanoclay hydrogen bonding and cross-linking with less amount of calcium ions. The Biowork pen ensures better mixing effectiveness for the kaolin-dispersed AL-CH hydrogels (evident from computational fluid dynamics study, aluminosilicate nanoclay mapping and 3D printing of complex multilayered structures) than the conventional mixing process. Two different cell lines (osteoblast and fibroblast) introduced during large area multilayered 3D bioprinting have confirmed the suitability of such multicomponent bioinks for in vitro even tissue regeneration. The effect of kaolin to promote uniform growth and proliferation of the cells throughout the bioprinted gel matrix is more significant for this advanced pen-type extruder processed samples.

Trend and Determinants of Fertility in a Declining Fertility Regime: A Study of Rural Uttar Pradesh.

This study is an attempt to examine the socio-economic and demographic differentials in children ever born and desire for additional child in rural Uttar Pradesh using the empirical data set. Two stage stratified random sampling procedure was applied to select the respondents from ten villages of rural Raebareli district. Total 460 women having with at least one child below ten years of age were interviewed during September 2016 to February 2017. Children ever born (CEB) and desire for another child were the main outcome variables of the study. One-way ANOVA and chi-square test were used in the bi-variate analysis. Poisson regression and logistic regression were used in multivariate analysis. The mean children ever born in the study area was 2.7 children per women and 64.7 per cent of fecund women who had two children and not currently pregnant had no desire for another child. Women's education, economic status of the household, autonomy index and higher caste group were significantly negatively associated with the children ever born in the study area. The desire for another child was significantly higher among those who had only daughters (OR = 1.99; p < 0.01). In conclusion, more emphasis needs to be placed on messages conveyed through the mass media, addressing the benefits of small family size and family planning, and programs that focus on reduction in newborn mortality could also be considered, which would also help to reduce fertility.

Nanodiamond enhanced mechanical and biological properties of extrudable gelatin hydrogel cross-linked with tannic acid and ferrous sulphate.

BACKGROUND: The requirements for cell-encapsulated injectable and bioprintable hydrogels are extrusion ability, cell supportive micro-environment and reasonable post-printing stability for the acclimatization of the cells in the target site. Detonation nanodiamond (ND) has shown its potential to improve the mechanical and biological properties of such hydrogels. Enhancing the performance properties of natural biopolymer gelatin-based hydrogels can widen their biomedical application possibilities to various areas including drug delivery, tissue engineering and 3D bioprinting. METHOD: In this study, natural cross-linker tannic acid (TA) is used along with ferrous sulphate (FS) to optimize the swelling and disintegration of extrudable and 3D printable gelatin hydrogels. The amounts of TA and FS are restricted to improve the extrusion ability of the gels in 3D printing. Further, ND particles (detonation type) are dispersed using twin screw extrusion technology to study their effect on mechanical and biological properties of the 3D printing hydrogel. RESULTS: The improved dispersion of ND particles helps to improve compressive strength almost ten times and dynamic modulus three times using 40 mg ND (2% w/w of gelatin). The surface-functional groups of detonation ND also contributed for such improvement in mechanical properties due to higher interaction with the hydrogel matrix. The stability of the hydrogels in water was also improved to 7 days. Four times improvement of the cell growth and proliferation was observed in ND based hydrogel. CONCLUSION: The cell-supportive nature of these moderately stable and extrudable ND dispersed gelatin hydrogels makes them a good candidate for short term regenerative applications of cell-encapsulated injectable hydrogels with better mechanical properties.

Multimorbidity as a Risk Factor of Elder Abuse/Mistreatment in India: A Cross-Sectional Study.

Globally, elder abuse/mistreatment is a common form of violence against the elderly. This study examines the association between multimorbidity and abuse/mistreatment among the elderly population (60+) in India. Cross-sectional data from the United Nations Population Fund (UNFPA)'s "Building Knowledge Base on Population Aging in India" (BKPAI, 2011) was analyzed. We generated a multimorbidity variable by combining 20 self-reported diagnosed chronic diseases. Bivariate analysis was used to understand the sample distribution and prevalence estimation of elder abuse/mistreatment by multimorbidity and state. Furthermore, multilevel mixed-effect logistic regression was used to examine the association between multimorbidity and elder abuse/mistreatment. The overall prevalence of elder abuse/mistreatment in the study population is 11.4%. The prevalence of elder abuse/mistreatment among elderly with no chronic diseases is 6.01% which increases to 22.7% among elderly with four or more chronic diseases. Furthermore, the result from the multilevel mixed-effect logistic regression showed a close association between multimorbidity and elder abuse. Elderly with two, three, and four or more chronic diseases are 3.02 (CI = 2.33, 3.91, p < .000), 4.16 (CI = 3.02, 5.74, p < .000), and 5.06 (CI = 3.50, 7.31, p <.000) times more likely to experience abuse/mistreatment than elderly with no chronic diseases, respectively. In specific, this association is stronger for the elderly population residing in the urban areas. Furthermore, economic status and educational attainment have a protective role in determining elder abuse/mistreatment in India. In conclusion, multimorbidity has emerged as a significant risk factor of elder abuse/mistreatment in India. Measures to prevent elder abuse should consider the role of multimorbidity.

Seasonal variation and mobility of trace metals in the beach sediments of NW Borneo.

Miri city has a dynamic coastal environment, mainly influenced by intensive sedimentation from the Baram River and excessive trace metal loading by the Miri River, which are significant environmental concerns. As the mobility, bioavailability, and toxicity of the trace metals in the sediments are largely controlled by their particulate speciation, the modified BCR sequential extraction protocol was applied to determine the particulate speciation of trace metals in the coastal sediments of Miri, to unravel the seasonal geochemical processes responsible for known observations, and to identify possible sources of these trace metals. The granulometric analysis results showed that littoral currents aided by the monsoonal winds have influenced the grain size distribution of the sediments, enabling us to divide the study area into north-east and south-west segments where the geochemical composition are distinct. The Cu (>84%) and Zn (82%) concentrations are predominantly associated with the exchangeable fraction, which is readily bioavailable. Pb and Cd are dominant in non-residual fractions and other metals viz., Fe, Mn, Co, Ni, and Cr are dominant in the residual fraction. Using Pearson's correlation and factor analysis, the major mechanisms controlling the chemistry of the sediments are identified as association of Cu and Zn with fine fraction sediments, sulphide oxidation in the SW segment of the study area, atmospheric fallout of Pb and Cd in the river basins, precipitation of dissolved Fe and Mn supplied from the rivers and remobilization of Mn from the coastal sediments. Based on various pollution indices, it is inferred that the coastal sediments of NW Borneo are contaminated with Cu and Zn, and are largely bioavailable, which can be a threat to the local aquatic organisms, coral reefs, and coastal mangroves.

Progress of South East Asian Region countries towards achieving interim End TB strategy targets for TB incidence and mortality: a modelling study.

OBJECTIVES: In 2018, the World Health Organisation (WHO) reported that almost 10 million people worldwide had tuberculosis (TB). The majority of the TB cases were concentrated in South East Asian Region (SEAR) countries. To curb this burden, the WHO has set interim targets to reduce TB incidence by 50% and mortality by 75% by 2025 (interim targets of the 'End TB' strategy). Hence, this study was conducted to determine the progress of SEAR countries towards reaching these interim targets for TB incidence and mortality. METHODS: Secondary data analysis was performed using information from the WHO Global Health Observatory and Global Health Estimates for all SEAR countries. ARIMA modelling was used to forecast TB incidence and TB mortality from 2000 to 2025. Joinpoint regression was performed to determine the average annual percent change. RESULTS: Based on the current trend, we estimated that the incidence and mortality of TB in SEAR countries will decline to 3.7 million (4.37 million in 2018) and 495,280 (650,979 in 2016) by 2025, respectively. Projected age-standardised TB incidence showed a significant decline in only four SEAR countries, with the maximal decline in Myanmar (47.3%) followed by India (23.4%) compared with 2015 estimates. Age-standardised TB mortality showed a declining trend in six SEAR countries, with the maximum decline found in Myanmar (60.3%) followed by Thailand (39.0%). CONCLUSIONS: Current study findings show that it is highly unlikely for most SEAR countries to achieve the WHO recommended interim End TB targets of 50% reduction in incidence and 75% reduction in mortality by 2025.

Impact of change in household environment condition on morbidity in India: Evidence from longitudinal data.

BACKGROUND: Household environment condition is an important predictor of morbidity of the household members. Without forming a healthy household environment, creating a healthy population is not possible. In this background, this study assesses the impact of change in household environment conditions on morbidity. METHODS: For the empirical analysis purpose of this study, we used two waves of longitudinal data from India Human Development Survey (2004-05, 2011-12). This study is based on 34131 re-contacted households in 2011-12 from the base year 2005. The bivariate and ANOVA tests were performed to assess any short-term morbidity (diarrhoea, fever and cough) with respect to change in household environment condition from 2005 to 2011. The multivariate linear regression was performed to assess the impact of change in household environment conditions on morbidity. The multinomial logistic regression was used to assess the impact of change in household environment condition on change in morbidity. RESULTS: The results from multivariate linear regression have shown that the share of household members fell sick due to any short-term morbidity (ASM) was significantly lower (ß = -0.060, P<0.001) among the households who lived in clean environment condition in both the periods, 2004-05 and in 2011-12 as compared to those who were living in poor environment condition in both periods net of other socio-economic characteristics of the households. The share of household members fell sick due to any short-term morbidity has significantly declined (ß = -0.051, P<0.001) among the household whose household environment condition has changed from poor in 2004-05 to clean environment in 2011-12 as compared to the households who have lived in poor environment condition in both periods in 2004-05 and 2011-12. The results of adjusted percentage from multinomial logistic regression have shown that the household members who fell sick with ASM was remained higher (4.9%; P<0.05) among the households whose environment condition was remained poor in both years in 2005 and 2011 as compared to the other households (2.7%) who remained in the better-off condition in both years in 2005 and 2011. CONCLUSION: Considering the findings of the study, we suggest that ongoing government flagships programmes such as Swacch Bharat Mission (Clean India Mission), Pradhan Mantri Ujjwala Yojana (Prime Minister Clean Energy Scheme) and Pradhan Mantri Awas Yojana (Prime Minister Housing Scheme), and Jal Jeevan Mission (Improved Source of Drinking Water Scheme) should work in tandem to improve household environment conditions.

Molecular dynamics simulations of cognate and non-cognate AspRS-tRNAAsp complexes.

Aspartyl tRNA synthetase (AspRS), one of the 20 aminoacyl-tRNA synthetases, plays an important role in protein synthesis by catalyzing the aminoacylation reaction and synthesises Aspartyl-tRNA (tRNAAsp). A typical three-dimensional structure of AspRS comprises three distinct domains for the recognition of cognate tRNA and catalysis, namely, anti-codon binding domain/N-terminal domain, hinge domain and catalytic domain through their interactions with anti-codon loop, D-stem and acceptor arm of cognate tRNA, respectively. In this work, we have studied the structural characteristics of each domain of AspRS to understand the recognition mechanism of tRNAAsp using molecular dynamics simulations. The dynamics of AspRS-tRNAAsp complexes from E.coli (cognate and non-cognate), S.cerevisiae (cognate) and T.thermophilus (non-cognate) were compared to understand the differences in recognition of cognate and non-cognate tRNAs. Our results explain that the conformational changes associated with the recognition of tRNA occur only in the cognate complexes. Among the cognate complexes, the conformational changes in yeast AspRS are highly controlled during tRNAAsp recognition than that of in the E. coli AspRS. Moreover, the functional motions required for the tRNA recognition are observed only in the cognate complexes, and the conformational changes in AspRS and their recognition of tRNAAsp are organism specific.Communicated by Ramaswamy H. Sarma.

A mechanistic model to account for the Donnan and volume exclusion effects in ultrafiltration/diafiltration process of protein formulations.

Ultrafiltration/diafiltration (UF/DF) is a typical step in protein drug manufacturing process to concentrate and exchange the protein solution into a desired formulation. However, significant offset of pH and composition from the target formulation have been frequently observed after UF/DF, posing challenges to the stability, performance, and consistency of the final drug product. Such shift can often be attributed to the Donnan and volume exclusion effects. In order to predict and compensate for those effects, a mechanistic model is developed based on the protein charge, mass and charge balances, as well as the equilibrium condition across the membrane. The integrated UF/DF model can be used to predict both the dynamic behavior and the final outcome of the process. Examples of the modeling results for the pH and composition variation during the UF/DF operations are presented for two monoclonal antibody proteins. The model predictions are in good agreement with a comprehensive experimental data set that covers different process steps, protein concentrations, solution matrices, and process scales. The results show that significant pH and excipient concentration shifts are more likely to occur for high protein concentration and low ionic strength matrices. As a special example, a self-buffering protein formulation shows unique pH behavior during DF, which could also be captured with the dynamic model. The capability of the model in predicting the performance of UF/DF process as a function of protein characteristics and formulation conditions makes it a useful tool to improve process understanding and facilitate process development.

Strong structural phase sensitive rare-earth photoluminescence color flips in KLaF4:RE3+ (RE3+ = Eu3+, Er3+/Yb3+) nanocrystals.

Systematic and strong rare-earth photoluminescence (PL) color flips that are highly sensitive to structural phase transformation in KLaF4:RE3+ (RE3+ = Eu3+, Er3+/Yb3+) nanocrystals are demonstrated. Room-temperature wet chemical synthesis at various reaction times involves a systematic conversion from cubic (α, space group Fm3[combining macron]m) to hexagonal (ß, space group P6[combining macron]2m) polymorph nanocrystals of 4 to 8 nm sizes. The unusual down-conversion photoluminescence (DC-PL) asymmetric ratio of the hypersensitive transition (5D0→7F2, Red) to that of nearly invariant transition (5D0→7F1, Orange) (R/O from 1.2 to 3.0) of KLaF4:Eu3+ is substantially affected by the site-selective occupancy and local symmetry around the Eu3+ ions, according to crystal packing transformation. The NIR excited up-conversion photoluminescence (UC-PL) of Er3+ ions produces a strong color flip from green (2H11/2 and 4S3/2) to red (4F9/2) dominated emissions based on their cubic or hexagonal crystal packing. The site occupancy and phonon energies strongly influence various nonlinear energy transfer mechanisms within RE3+ ion energies and the results are explained accordingly. The present study substantially reveals the local host effects and these two distinct polymorph nanoparticles can be potentially utilized for color-specific studies related to applications such as color-specific biological in vitro and in vivo imaging and other optoelectronic device applications.

Geochemical behaviour and risk assessment of trace elements in a tropical river, Northwest Borneo.

By convention, dissolved trace elements in the river water are considered to be the fraction that passes through a 0.45 µm filter. However, several researchers have considered filtration cut-off other than 0.45 µm for the separation of dissolved trace elements from particulate fraction. Recent research indicated that trace elements could exist in particulate form as colloids and natural nanoparticles. Moreover, the trace elements in the continental dust (aerosols) constitute a significant component in their geochemical cycling. Due to their high mobility, the trace elements in the micron and sub-micron scale have biogeochemical significance in the coastal zone. In this context, this study focuses on the highly mobile fraction of trace elements in particulates (<11 µm) and dissolved form in the Lower Baram River. A factor model utilizing trace elements in the dissolved and mobile phase in the particulates (<11 µm) along with water column characteristics and the partition coefficient (Kd) of the trace elements indicated a more significant role for manganese oxyhydroxides in trace element transport. Perhaps, iron oxyhydroxides play a secondary role. The factor model further illustrated the dissolution of aluminium and authigenic clay formation. Except for Fe and Al, the contamination risk of mobile trace elements in particulates (<11 µm) together with dissolved form are within the permissible limits of the Malaysian water quality standards during monsoon (MON) and postmonsoon (POM) seasons.

Oral Microbiome and Gingival Transcriptome Profiles of Ligature-Induced Periodontitis.

This investigation evaluated the relationship of the oral microbiome and gingival transcriptome in health and periodontitis in nonhuman primates (Macaca mulatta). Subgingival plaque samples and gingival biopsies were collected from healthy sites and at sites undergoing ligature-induced periodontitis. Microbial samples were analyzed with 16S amplicon sequencing to identify bacterial profiles in young (3 to 7 y) and adult (12 to 23 y) animals. The gingival transcriptome was determined with a microarray analysis and focused on the expression level of 452 genes that are associated with the development of inflammation and innate and adaptive immune responses. Of the 396 total operational taxonomic units (OTUs) identified across the samples, 81.8% were detected in the young group and 99.5% in the adult group. Nevertheless, 58 of the OTUs composed 88% of the signal in adults, and 49 OTUs covered 91% of the OTU readouts in the young group. Correlation analyses between the microbiome members and specific gingival genes showed a high number of significant bacteria-gene correlations in the young healthy tissues, which decreased by 75% in diseased tissues. In contrast, these correlations increased by 2.5-fold in diseased versus healthy tissues of adult animals. Complexes of bacteria were delineated that related to specific sets of immune genes, differing in health and disease and in the young versus adult animals. The correlated gene profiles demonstrated selected pathway overrepresentation related to particular bacterial complexes. These results provide novel insights into microbiome changes with disease and the relationship of these changes to specific gene profiles and likely biologic activities occurring in healthy and diseased gingival tissues in this human-like periodontitis model.

Ultrasonic Nanoemulsification of Cuminum cyminum Essential Oil and Its Applications in Medicine.

BACKGROUND AND STUDY: Cumin seed oil (extracted from Cuminum cyminum) has many applications but conclusive evidence of its therapeutic uses has not been presented. This study has explored the anticancer and antibacterial properties of the seed oil. METHODS: The cumin nanoemulsion was prepared with Tween 80 non-ionic surfactant employing ultra-sonication technology. The anticancer activity of the nanoscale-based emulsion was evaluated through cell viability (MTT), antiproliferation evaluation through clonogenic assay, and apoptosis through Annexin V-FITC assay. Agar well diffusion was used to study the antimicrobial activity, and this was supported by membrane integrity analysis. RESULTS: A thorough study of process parameters, aimed at obtaining the optimal surface concentration and emulsification time, was completed. GC-MS data indicated cumaldehyde as a major component. The resultant droplet diameter after a sonication time of 5 min was 10.4 ± 0.5 nm. MTT assay revealed the IC50 value at 1.5 µL/mL and the early induction of apoptosis was evident. Tongue carcinoma cell line treated with cumin nanoemulsion presented a diminished colony formation. The nanoemulsion exhibited significant antibacterial activity against S. aureus. A significant cytoplasmic leakage was observed on treatment with cumin nanoemulsion. The consequences of the analysis projected cumin as a potential component for cancer therapy. CONCLUSION: This study provides definitive evidence for cumin essential oil nanoemulsion as a legitimate plant-based medicine that can bypass the drawbacks of the present aggressive treatment of cancer, can overcome the antimicrobial resistance, and can also meet all prerequisites.

Bioaccumulation of trace metals in the coastal Borneo (Malaysia) and health risk assessment.

The concentration of nine trace elements were analyzed in the different tissue organs of commonly available crabs (Portunus sanguinolentus, Portunus pelagicus and Scylla serrate) and bivalve (Polymesoda erosa) species collected from the Miri coast, Borneo in order to evaluate the potential health risk by consumption of these aquatic organisms. Among the analyzed organs, metal accumulation was higher in the gill tissues. The essential (Cu and Zn) and non-essential (Pb and Cd) elements showed the highest (i.e. Zn) and lowest concentrations (i.e. Cd) in their tissue organs, respectively. The estimated daily intake and hazard indices of all metals in the muscle indicate that the measured values were below the provisional tolerable daily intake suggested by the joint FAO/WHO Expert Committee on Food Additives. Compared to Malaysian and international seafood guideline values the results obtained from the present study are lower than the permissible limits and safe for consumption.

Anticancer and antibacterial effects of a clove bud essential oil-based nanoscale emulsion system.

BACKGROUND AND PURPOSE: The essential oil derived from clove buds (Syzygium aromaticum) has been used as a chemopreventive agent in Ayurvedic medicine. The antiviral, antibacterial, and anticancer properties of its chemo-skeleton have motivated this study to explore its efficacy in pharmaceutics. METHODS: Nanoscale-based emulsions were prepared by employing a spontaneous emulsification technique through self-assembly using varying concentrations of Tween 20 and Tween 80 surfactants. Their physicochemical properties and stability were studied in order to choose an optimum formulation which was clear and stable. The cytotoxicity of the stable oil-based emulsion system was evaluated using MTT assay, colony formation assay, and Annexin V-FITC assay against the thyroid cancer cell line (HTh-7). RESULTS: All three methods verified apoptosis and reduction in cancer cell proliferation, making the formulation a promising candidate as an alternative cancer drug. The oil-based emulsion system was also tested for its antibacterial properties against Staphylococcus aureus. Membrane permeability studies proved its efficacy to permeate through cell membrane, thereby increasing the leakage of cytoplasmic contents. CONCLUSION: Many current treatments for cancers are aggressive yet ineffective. This study positions the clove bud-based nanoscale emulsion as a suitable candidate for further in vivo studies and trials as a cancer drug.

Diurnal and seasonal patterns of soil CO2 efflux from the Pichavaram mangroves, India.

The diurnal and seasonal variation of soil carbon dioxide (CO2) flux was measured in the Pichavaram mangrove forest, the Southeast coast of India from February 2016 to October 2016 using an automated soil CO2 flux chamber system. Maximum soil CO2 efflux reached at 14:00 h and minimum at 00:00 h. The surface soil CO2 concentration ranged from 375 to 532 ppm with the mean 405 ± 18 ppm. The daily soil CO2 flux varied from near zero to about 7 µmol m-2 s-1 with a mean value of 2.4 ± 1.3 µmol m-2 s-1. The highest seasonal CO2 efflux from soil was during the summer and premonsoon seasons, whereas low flux values were recorded during the monsoon season. Soil CO2 efflux values were highly correlated with soil temperature. Tidal inundation during monsoon season, extreme drought condition in summer, and unusual precipitation are the major factors controlling the soil CO2 flux.