Square wave anodic stripping voltammetric determination of metoclopramide in tablet and urine at carbon paste electrode.

A simple, reliable and selective square wave anodic stripping (SWAS) voltammetric method at carbon paste electrode (CPE) of metoclopramide hydrochloride (MCP) in pharmaceutical dosage forms (tablet) and in biological fluids (spiked and real urine samples) has been developed and evaluated. Different parameters such as medium, supporting electrolyte, pH, accumulation potential, scan rate, accumulation time and ionic strength, were tested to optimize the conditions for the determination of MCP. The adsorbed form is oxidized irreversibly under optimal conditions, viz., 0.4M HCl-sodium acetate buffer (pH approximately 6.2), 0.2M KCl, a linear concentration ranges from 0.067 to 0.336, 0.067 to 0.269 and 0.067 to 0.269 ng/mL of MCP, at accumulation times 60, 120 and 180 s, respectively, can be determined successfully. The interferences of some common excipients and some metal ions were studied. The standard addition method was used to determine the MCP in pure solutions, tablets and in biological fluids with satisfactory results. The data obtained are compared with the standard official method.

Abolishing cadmium toxicity in Chlorella vulgaris by ascorbic acid, calcium, glucose and reduced glutathione.

Addition of ascorbic acid, calcium (Ca2+), glucose and reduced glutathione (GSH) to the cadmium (Cd2+)-amended Kuhl medium significantly accelerated the growth and chlorophyll content of Chlorella vulgaris. This enhancement was found to be dose dependent. Their relative effectiveness against Cd2+ on growth and chlorophyll content has been arranged as GSH > Ca2+ > glucose > ascorbic acid. However, Ca2+ offered more protection against Cd2+ toxicity on protein contents, followed by ascorbic acid, GSH and glucose. The different concentrations of all amelioratives completely alleviated the toxicity of Cd2+ on total carbohydrates to exceed the control level. Photosynthetic oxygen evolution showed more response to Ca2+ and glucose in alleviating Cd2+ toxicity. On the other hand, at certain concentrations, GSH, Ca2+, ascorbic acid and glucose stimulated respiratory oxygen uptake over the control level. The uptake of Cd2+ dropped significantly in cultures supplemented with 20 microM ascorbic acid, glucose and GSH, while Ca2+ (30 microM) significantly lowered the Cd2+ uptake by 58% indicating that Ca2+ had a more antagonistic effect against Cd2+.