Exogenous Putrescine Changes Biochemical (Antioxidant Activity, Polyphenol, Flavonoid, and Total Phenol Compounds) and Essential Oil Constituents of Salvia officinalis L.

Polyamines are small polycationic molecules containing amines that are present in almost all cells of living organisms and act in a wide range of physiological processes, growth, and development, biological and protection of cells against free radicals. This research is based on principal component analysis (PCA) and calculation of selection criteria (SC) to investigate the effect of foliar spraying of polyamine putrescine on essential oil yield, essential oil compounds, antioxidant activity, and biochemical compounds (polyphenol, flavonoid, and total phenol compounds) of Salvia officinalis. The treatments used included four levels of putrescine, Put (Control: 0, Put1: 500, Put2: 1000, and Put3: 1500 mg L-1 ) with five replications. Based on our results, four factors had eigenvalues≥1 and showed a cumulative variance percentage of 92.57 % by applying different concentrations of putrescine. According to the results of this research, putrescine had significant effects on the amount of total phenolic compounds, flavonoids, and antioxidant activity. The best attention to improving the essential oil yield of sage was 1000 mg L-1 . The crucial essential oil compounds of different Put treated sage were: cis-thujone (35.34 %), camphor (15.60 %), trans-thujone (9.90 %), 1,8-cineole (9.46 %), α-humulene (3.85 %), viridiflorol (3.62 %), camphene (3.58 %), α-pinene (3.50 %), ß-pinene (2.78 %), and limonene (1.23 %). The results showed that the amount of total phenol, the phenolic composition of catechin, and the antioxidant activity of sage plant extract increased significantly when putrescine was used at 1000 mg/liter. Results can use the current research to optimize the production management of medicinal plants and improve the quality of their products. In addition, the advantage of using putrescine is that it increases antioxidants and reduces oxidative damage, and can replace medicinal plants as suitable natural preservatives, thus improving food quality and safety.

Evaluation of pharmaceutical compounds at vegetative and reproductive growth stages of Marrubium vulgare, a medicinal plant to cleanse the body.

Marrubium vulgareis a plant with high bioactive potential. The rich bioactive compounds of medicinal horehound, as well as their antioxidant activity have led to its wider application in the pharmaceutical, cosmetic, and food industries. The present work aimed to determine the phenolic compounds, total phenols, flavonoids, and antioxidant activity at vegetative and reproductive growth stages of Marrubium vulgare. The experiments were performed in Eram Garden with five replications and using SPSS software (v. 13) and Mann-Whitney U-Test at p ≤ 0.01. The total content of flavonoids and main phenolic compounds increased when plants collected at vegetative stage. On the other hand, the highest vanillin content was observed in full flowering stage. Overall, this study can provide useful information about the best harvest period for horehound to produce the desired compounds for use in the food, pharmaceutical, and plant industries.

Comparison monoterpene and sesquiterpene, herbage yield of Satureja hortensis, Thymus vulgaris, and Salvia officinalis from the Fars province of Iran.

Recently, numerous side effects of synthetic drugs have led to using medicinal plants as a reliable source of new therapy. Pain is a global public health problem with a high impact on life quality and a huge economic implication, becoming one of the most important enemies in modern medicine. The medicinal use of plants as analgesic or antinociceptive drugs in traditional therapy is estimated to be about 80% of the world population. The lamiaceae family, one of the most important herbal families, incorporates a wide variety of plants with biological and medical applications. In this study, the main active compounds of Thymus vulgaris (thymol (51.59%), p-cymene (15.41%), carvacrol (4.52%) and γ-terpinene (12.63%), Salvia officinalis; cis-thujone (38.05%), camphor (16.08%), 1,8-cineole (11.97%), trans-thujone (5.88%), camphene (4.79%), α -pinene (4.69%) and carvacrol (50.68%), γ-terpinene (34.44%) and α-terpinene (3.72%) in Satureja hortensis was determined. The highest EO contents (w/w %) was obtained in the plants of summer savory (2.05%), followed by the garden thyme (1.83%) and common sage (0.55%). It is necessary to identify the most effective compounds in order to extract or synthesise in pure form for future use in the pharmaceutical industry. Also, might be used for isolation of potentially active compounds from some of these medicinal plants in future and formulation of commercial therapeutic agents.

Annual Phytochemical Variations and Antioxidant Activity within the Aerial Parts of Lavandula angustifolia, an Evergreen Medicinal Plant.

Acknowledging the importance of medicinal compounds, flavonoids, and phenolic acids in plants and human health; understanding the best time to harvest plants to get the most potentially therapeutic biological activity; and predicting and controlling the quality of medicinal plants are very useful. The rich chemical composition of medicinal lavender, as well as its antioxidant activity, has led to its wide application in the pharmaceutical, cosmetic, and food industries. In the current research, seasonal differences in phenolic content; the main constituents of essential oil, essential oil yield, and antioxidant activity; and flavonoid content were evaluated. In this investigation, for all the investigated factors, three replications were considered as completely randomized designs. The predominant natural components were α-pinene, camphene, 1,8-cineole, limonene, camphor, borneol, cryptone, isobornyl formate, cumin aldehyde, carvone, caryophyllene oxide, and epi-α-cadinol. The highest rate of 1,8-cineole was seen in summer, while camphor and borneol showed their highest percentages in the cold season. Some of the compounds such as caryophyllene oxide, isobornyl formate, and cumin aldehyde showed their highest amounts in the spring with the activation of some important plant enzymes such as peroxidase. The total content of flavonoids and some phenolic compounds (coumarin and p-coumaric acid) increased when plants received sunlight for a longer time than in the colder to warmer seasons. On the other hand, increases in chlorogenic acid and vanillin were observed in spring. Also, the highest antioxidant activity was observed in lavender, which was accompanied by an increase in the important phenolic composition of rosmarinic acid in the cold season. Generally, this investigation provides beneficial evidence about the most appropriate harvest time for lavender to produce the most favorable constituents for optimization in food, pharmaceutical, and herbal products.

Seasonal variation on the major bioactive compounds: total phenolic and flavonoids contents, and antioxidant activity of rosemary from shiraz.

The rich bioactive compounds of medicinal rosemary, as well as their antioxidant activity has led to its wider application in the pharmaceutical, cosmetic and food industries. In the present work, seasonal variations of phenolic, flavonoid content and antioxidant activity were evaluated. This experiment was performed based on a complete randomised design with three replications. The total content of flavonoids and some phenolic compounds (caffeic acid and quercetin) increased when plants received sunlight for a longer period of time than the colder seasons. On the other hand, the highest antioxidant activity was observed in rosemary, which was accompanied by an increase in the important phenolic composition of rosmarinic acid in the cold season. Overall, this study can provide useful information about the best harvest period for rosemary to produce the desired compounds for use in the food, pharmaceutical and plant industries.

The effect of time and temperature on shelf life of essential oil composition of Teucrium polium L.

Teucrium polium has been used to treat gastrointestinal disorders, inflammation, diabetes and rheumatism. This plant has beneficial therapeutic properties. In this research the changes in the composition of essential oils from the aerial parts of Teucrium polium were determined at different temperatures and times. The essential oil of air-dried samples was obtained by hydro distillation and analyzed by gas chromatography/mass spectrometry (GC/MS). Changes in essential oils compositions were detected during storage for 3 months in refrigerator, freezer, and at room temperature. The results indicated that at room temperature, the proportions of the compounds with lower boiling temperatures such as Cis-Verbenol (2.11-1.78%) and Germacrene D (1.56-0.94%) were decreased significantly. Furthermore, the essential oil composition showed the least alterations and Teucrium polium L. kept its primary quality when stored at low temperatures, particularly in refrigerator and freezer (Except: α-Pinene, ß-Pinene and Limonene).

The effect of harvest time on the natural product of Rosmarinus officinalis L. from South Iran (Fars province).

The medicinal composition of rosemary essential oil has led to its widespread use in the food, cosmetic, health and herbal industries. In the present work, seasonal variations of essential constituents of essential oil, essential oil yield were evaluated. The predominant components of rosemary were: camphene, limonene, α-pinene, camphor, borneol, linalool, 1,8-cineole, (E)- caryophyllene and bornyl acetate. The highest rate of linalool was seen in summer, while camphor, limonene and borneol showed the highest percentage in cold season. Some of the compounds such as α-pinene, camphene, bornyl acetate and (E)- caryophyllene in the spring with the activation of some important plant enzymes such as peroxidase showed the highest amount. It is hoped that this research will provide very useful information about the best time to harvest the valuable rosemary plant and can be useful for the health, food and herbal industries that use this plant.

Polyphenolic contents and antioxidant activities of aerial parts of Salvia multicaulis from the Iran flora.

Folk medicine sometimes involves the use of salvia species for therapeutic purposes. Polyphenols with the highest amounts included rosmarinic acid (7.358mg/g), catechin (1.5 mg/g), vanillin (1.00 mg/g), chlorogenic acid (0.53 mg/g), quercetin (0.16 mg/g), and p-coumaric acid (0.015). Furthermore, the results showed that S. multicaulis has a high content of total phenol (4.39 mg/g) and DPPH activity (8.44 mg/g). Salvia multicaulis could be potentially used as a medicinal plant because of its antioxidant activity and polyphenol content.

Antioxidant activity and essential oil composition of Satureja hortensis L. as influenced by sulfur fertilizer.

BACKGROUND: The quantity and quality of essential oils in plants can be influenced by various factors, including genetic traits, agricultural practices and environmental conditions such as nutrient availability. Macronutrients such as sulfur (S) are among the major factors influencing plant growth and development. Therefore this study was carried out to determine the effect of S fertilization at three levels (0, 0.05 and 0.1 g S kg(-1) soil) on the essential oil composition and antioxidant activity of Satureja hortensis L. RESULTS: Application of 0.05 g S kg(-1) soil increased the levels of α-terpinene, p-cymene, myrcene, α-thujene and α-pinene but decreased the level of γ-terpinene in the essential oil. This S application was most suitable for obtaining higher proportions of α-terpinene, p-cymene, myrcene, α-thujene and α-pinene, while application of 0.1 g S kg(-1) soil was preferable for carvacrol. γ-Terpinene was most abundant in the control. All extracts showed a considerable DPPH-inhibitory effect, with IC50 ranging from 0.720 g L(-1) in the control to 0.363 g L(-1) with application of 0.1 g S kg(-1) soil. The maximum antioxidant effect was observed with application of 0.1 g S kg(-1) soil. CONCLUSION: The results revealed that the use of chemical fertilizers such as S could improve the antioxidant activity of plant extracts significantly. Studying the secondary plant metabolites, mainly essential oils, is an interesting research area, so further studies are recommended to determine the effect of chemical fertilizers on the composition and antioxidant activity of essential oils of other aromatic plants.