[Evaluation of cyto- and genotoxic action of ferronanomagnetic and constant magnetic field in in vivo system].

Cyto- and genotoxic effects of nanoparticles on the basis of FM, CMF or their combination have been studied in AKE cells, BM cells of erythroid line, and peripheral blood lymphocytes with the use of MN test and "DNA-comet" assay. It has been shown that expression of mentioned effects is related to FM concentration and duration of tested agent action. It has been also demonstrated that action of CMF alone in the studied cells did not cause any changes in cell architectonics or affect MN counts which are associated with DNA damage. When FM and CMF were used in combination there has been observed the phenomenon of induction of CMF action with FM nanoparticles. The obtained results allow recommend MN test and "DNA-comet" assay as the markers of genome stability in the tests of genotoxic effects of nanomaterials for development of vector nanosystems.

[Evaluation of biological effects and possible mechanisms of action of static magnetic field].

Modern views on mechanisms of interaction between static magnetic field and cells or cellular structures are reviewed. An analysis of the data about possible biotropic effects of this factor was performed. The emphasis was put on the analysis of the studies in which moderate (0.1-1 T) static magnetic fields were used, because such fields are used for targeted delivery ofmagnetosensitive nanocomposites in development of new strategies in target therapy of patients with malignant neoplasms. Based on available data it was concluded that the primary cause of changes in cells after incubation in external static magnetic field is disruption of free radical metabolism and elevation of their concentration. Such disruption causes oxidative stress, and, as a result, damages ion channels, leading to changes in cell morphology and expression of different genes and proteins, and also changes in apoptosis and proliferation.

[Visualization of iron nanoparticles accumulation and distribution features in sensitive and resistant to antitumor drugs human breast cancer cells after different time intervals of cultivation with liposomal ferromagnetic].

It was shown in vitro that visualization of iron nanoparticles by histochemical Lilly method, modified by authors for cytomorphological studies, could be a certain control of ferromagnetic income to human breast cancer MCF-7 cells with different sensitivity to antitumor drugs (doxorubicin and cisplatin). The form of the visualized iron nanoparticles, their localization and distribution towards intracellular structures was studied. It was shown that localization, dynamics of accumulation and release of ferromagnetic from cells were connected to their sensitivity to antitumor drugs and time of incubation with iron nanoparticles.

[Complex estimation of proliferative activity of epithelial cells of the large intestine damaged by polyps and cancer]. / Kompleksna otsinka proliferatyvnoï aktyvnosti klityn epiteliiu slyzovoï obolonky tovstoï kyshky vrazhenoï polipamy ta rakom.

Peculiarities of mitotic regime and expression of proliferating cell nuclear antigen were investigated in 18 polyps and 35 cases of colorectal cancer. Direct relationship between spectrum and degree of manifestation of proliferative activity, level of morphological malignant tumors and accumulation of oncopathology in the patient pedigrees was established.

[The characteristics of protein p53 expression in the mucosal epithelium of the large intestine affected by polyps and cancer]. / Osoblyvosti ekspresiï bilka p53 v epiteliï slyzovoï obolonky tovstoï kyshky, vrazhenoï polipamy ta rakom.

The direct dependence between p53 expression in epithelial cells and the level of morphological malignant neoplasm was established as the result of investigation of expression of this biomolecular marker in 12 polyps and 35 colorectal cancer. It was shown that family cancer syndrome 1 type was observed in pedigrees of majority patients with p53 positive cancers.

[The clinico-genetic characteristics of the origin and manifestation of colorectal cancer]. / Kliniko-henetychni osoblyvosti vynnyknennia ta manifestatsiï kolorektal'noho raku.

A clinical and genealogical investigation has been carried out in 169 subjects of both sexes, presenting with colorectal oncopathology. Particular features of the colorectal carcinoma in the patients have been recognized. The frequency of the tumours spread in the relatives of the probands is determined as is the spectrum of aggregation of large intestine carcinoma with neoplasia of other genesis in the pedigrees. The data obtained suggest an important contribution of genetic factors into the origination of colorectal carcinoma.

[A densitometric analysis of the chromatin structural characteristics of the nuclei in the peripheral blood lymphocytes of breast cancer patients]. / Densytometrychnyi analiz strukturnykh osoblyvostei khromatynu iader limfotsytiv peryferiinoï krovi u khvorykh na rak molochnoï zalozy.

Alterations in optical structural characteristics of nuclear chromatin, in comparison with healthy individuals (10) and patients with fibroadenoma (29), were detected in 57 patients with a breast cancer by densitometric investigation of peripheral blood lymphocytes. The degree of these alterations are closely associated with the level of malignancy in the initial neoplasia and the aggregation of oncopathology in pedigrees.