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1.
Biol Res ; 48: 12, 2015 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-25762127

RESUMO

BACKGROUND: In the present study, we examined the inhibitory effects of a methanolic extract, dichloromethane fraction, water layer, and polyhydroxylated sterols (1-4) isolated from the Vietnamese starfish Protoreaster nodosus on pro-inflammatory cytokine (IL-12 p40, IL-6, and TNF-α) production in LPS-stimulated bone marrow-derived dendritic cells (BMDCs) using enzyme-linked immunosorbent assays (ELISA). RESULTS: The methanolic extract and dichloromethane fraction exerted potent inhibitory effects on the production of all three pro-inflammatory cytokines, with IC50 values ranging from 0.60 ± 0.01 to 26.19 ± 0.64 µg/mL. Four highly pure steroid derivatives (1-4) were isolated from the dichloromethane fraction and water layer of P. nodosus. Potent inhibitory activities were also observed for (25S) 5α-cholestane-3ß,4ß,6α,7α,8ß,15α,16ß,26-octol (3) on the production of IL-12 p40 and IL-6 (IC50s = 3.11 ± 0.08 and 1.35 ± 0.03 µM), and for (25S) 5α-cholestane-3ß,6α,8ß,15α,16ß,26-hexol (1) and (25S) 5α-cholestane-3ß,6α,7α,8ß,15α,16ß,26-heptol (2) on the production of IL-12 p40 (IC50s = 0.01 ± 0.00 and 1.02 ± 0.01 µM). Moreover, nodososide (4) exhibited moderate inhibitory effects on IL-12 p40 and IL-6 production. CONCLUSION: This is the first report of the anti-inflammatory activity from the starfish P. nodosus. The main finding of this study is the identification oxygenated steroid derivatives from P. nodosus with potent anti-inflammatory activities that may be developed as therapeutic agents for inflammatory diseases.


Assuntos
Anti-Inflamatórios/análise , Células Dendríticas/efeitos dos fármacos , Subunidade p40 da Interleucina-12/farmacologia , Interleucina-6/farmacologia , Estrelas-do-Mar/química , Fator de Necrose Tumoral alfa/farmacologia , Animais , Sobrevivência Celular/efeitos dos fármacos , Ensaio de Imunoadsorção Enzimática , Concentração Inibidora 50 , Subunidade p40 da Interleucina-12/análise , Interleucina-6/análise , Lipopolissacarídeos , Camundongos Endogâmicos C57BL , Cultura Primária de Células , Esteroides/administração & dosagem , Fator de Necrose Tumoral alfa/análise , Vietnã
2.
Biol. Res ; 48: 1-9, 2015. ilus, graf, tab
Artigo em Inglês | LILACS | ID: biblio-950776

RESUMO

BACKGROUND: In the present study, we examined the inhibitory effects of a methanolic extract, dichloromethane fraction, water layer, and polyhydroxylated sterols (1-4) isolated from the Vietnamese starfish Protoreaster nodosus on pro-inflammatory cytokine (IL-12 p40, IL-6, and TNF-α) production in LPS-stimulated bone marrow-derived dendritic cells (BMDCs) using enzyme-linked immunosorbent assays (ELISA). RESULTS: The methanolic extract and dichloromethane fraction exerted potent inhibitory effects on the production of all three pro-inflammatory cytokines, with IC50 values ranging from 0.60 ± 0.01 to 26.19 ± 0.64 µg/mL. Four highly pure steroid derivatives (1-4) were isolated from the dichloromethane fraction and water layer of P. nodosus. Potent inhibitory activities were also observed for (25S)5α-cholestane-3ß,4ß,6α,7α,8ß,15α,16ß,26-octol (3) on the production of IL-12 p40 and IL-6 (IC50s = 3.11 ± 0.08 and 1.35 ± 0.03 µM), and for (25S) 5α-cholestane-3ß,6α,8ß,15α,16ß,26-hexol (1) and (25S)5α-cholestane-3ß,6α,7α,8ß,15α,16ß,26-heptol (2) on the production of IL-12 p40 (IC50s = 0.01 ± 0.00 and and 1.02 ± 0.01 µM). Moreover, nodososide (4) exhibited moderate inhibitory effects on IL-12 p40 and IL-6 production. CONCLUSION: This is the first report of the anti-inflammatory activity from the starfish P. nodosus. The main finding of this study is the identification oxygenated steroid derivatives from P. nodosus with potent anti-inflammatory activities that may be developed as therapeutic agents for inflammatory diseases.


Assuntos
Animais , Camundongos , Estrelas-do-Mar/química , Células Dendríticas/efeitos dos fármacos , Interleucina-6/farmacologia , Fator de Necrose Tumoral alfa/farmacologia , Subunidade p40 da Interleucina-12/farmacologia , Anti-Inflamatórios/análise , Esteroides/administração & dosagem , Vietnã , Ensaio de Imunoadsorção Enzimática , Sobrevivência Celular/efeitos dos fármacos , Lipopolissacarídeos , Interleucina-6/análise , Fator de Necrose Tumoral alfa/análise , Concentração Inibidora 50 , Subunidade p40 da Interleucina-12/análise , Cultura Primária de Células , Camundongos Endogâmicos C57BL
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