RESUMO
The aim of this study was to examine the effect of polymorphisms in the cytochrome P450 (CYP) 2C19 gene (CYP2C19) on the Helicobacter pylori eradication rate in Brazilian patients with functional dyspepsia. Adults diagnosed with functional dyspepsia based on the ROME III criteria and infected with H. pylori were recruited to this study. The patients were subjected to gastrointestinal endoscopy and the H. pylori status was defined when both urease test and histopathology results were negative or positive. The patients were treated with proton pump inhibitor-based triple therapy (omeprazole, amoxicillin, and clarithromycin). CYP2C19*2 and CYP2C19*3 were genotyped by polymerase chain reaction-restriction fragment length polymorphism. One hundred and forty-eight patients (81.8% women) with a mean (± SD) age of 46.1 (12.2) years were included in this study. Based on the CYP2C19 genotypes, the patients were classified as homozygous extensive metabolizer (HomEM; 67.6%), heterozygous extensive metabolizer (HetEM; 26.3%), or poor metabolizer (PM; 6.1%). The H. pylori eradication rates in patients with HomEM, HetEM, and PM were 85.0, 89.7, and 100.0% (P = 0.376), respectively. The included study population comprised a high frequency of patients carrying the HomEM genotype. Although the genotypes of CYP2C19 variants were not statistically significant, the results of this study suggest a possible effect of the PM genotype on the efficacy of H. pylori eradication.
Assuntos
Citocromo P-450 CYP2C19/genética , Dispepsia/genética , Infecções por Helicobacter/genética , Helicobacter pylori/genética , Adulto , Idoso , Amoxicilina/administração & dosagem , Brasil , Claritromicina/administração & dosagem , Dispepsia/tratamento farmacológico , Dispepsia/microbiologia , Endoscopia Gastrointestinal , Feminino , Genótipo , Infecções por Helicobacter/tratamento farmacológico , Infecções por Helicobacter/microbiologia , Helicobacter pylori/efeitos dos fármacos , Helicobacter pylori/patogenicidade , Humanos , Inativação Metabólica/genética , Masculino , Pessoa de Meia-Idade , Omeprazol/administração & dosagem , Polimorfismo de Nucleotídeo ÚnicoRESUMO
The immediately postbirth extra uterine adaptation is the most important cause of death in the first two hours of life. In all risky cases, it is necessary to effect efficient and on time techniques of newborn resuscitation, because dubitation or delay may be very dangerous for the infant. In Italy courses of equipment in newborn resuscitation are regularly performed, but an excellent level of technique can be obtained only with continuous daily practice. Then, particularly in little hospitals where it is unusually necessary to act resuscitation on a newborn, courses of simulation for medical and nursering staff would be opportune to prevent neonatal handicap and to deal with the professional liability in the best way. The Italian current jurisprudence, in fact, has slowly confined the application of 2236 article of Civil code about professional liability in particularly difficult efforts. The Italian law asserts that a professional specialist is trained to be able resolve any type of problem among those of his specialistic competence, even if technically very difficult. It should be opportune to train health staff with practical exercises, in order to obtain complete technical skills in all neonatal centers.
Assuntos
Conhecimentos, Atitudes e Prática em Saúde , Responsabilidade Legal , Padrões de Prática Médica , Competência Profissional , Ressuscitação , Serviços de Saúde da Criança/legislação & jurisprudência , Humanos , Recém-Nascido , ItáliaRESUMO
The cellular isoform of the prion protein (PrPc) is a glycosylphosphatidylinositol-anchored glycoprotein, normally expressed in neural and non-neural tissues, including skeletal muscle. In transmissible spongiform encephalopathies, or prion diseases, PrPc, which is soluble in nondenaturing detergent and sensitive to proteinase K (PK)-treatment, represents the molecular substrate for the production of a detergent-insoluble and PK-resistant isoform, termed PrP(Sc). In human prion diseases, PrP(Sc) accumulation occurs only in brain tissues, with the exception of new variant Creutzfeldt-Jakob disease, where PrP(Sc) is also detected in lymphoid tissues. Increased amounts of prion protein expression and deposition have been described in pathological muscle fibers of two human muscle disorders, called sporadic inclusion-body myositis (s-IBM) and hereditary inclusion-body myopathy, but it is unknown whether accumulated prion protein reflects normal PrPc or PrP(Sc). We investigated the biochemical characteristics of prion protein in normal human muscle, s-IBM, other inflammatory myopathies and denervation atrophy. We report that 1) both the glycoform profile and size of the normal muscle PrPc are different from those of human brain PrPc; 2) in addition to s-IBM, increased PrPc expression is seen in polymyositis, dermatomyositis and neurogenic muscle atrophy, but PrPc glycoforms are unchanged; 3) only the normal PrPc isoform, and not PrP(Sc), is detected in s-IBM. The present results exclude that s-IBM is a prion disease.
Assuntos
Encéfalo/metabolismo , Músculo Esquelético/metabolismo , Músculo Esquelético/patologia , Atrofia Muscular/metabolismo , Doenças Musculares/metabolismo , Miosite de Corpos de Inclusão/metabolismo , Proteínas PrPC/metabolismo , Adulto , Encéfalo/citologia , Encéfalo/patologia , Humanos , Inflamação , Leucócitos/citologia , Leucócitos/metabolismo , Leucócitos/patologia , Pessoa de Meia-Idade , Denervação Muscular , Fibras Musculares Esqueléticas/metabolismo , Fibras Musculares Esqueléticas/patologia , Músculo Esquelético/citologia , Atrofia Muscular/patologia , Doenças Musculares/patologia , Miosite de Corpos de Inclusão/patologia , Proteínas PrPC/análise , Valores de ReferênciaRESUMO
Simultaneous bilateral plantar sympathetic skin response (SSR) was studied in 25 patients with early stage idiopathic Parkinson's disease (IPD), characterized by monolateral motor involvement (Hoehn and Yahr, stage <2) and without clinical evidence of autonomic dysfunctions. Thirteen (mean age: 68.69 +/- 7.70, range 55-76) had extrapyramidal clinical signs only at the left body side, 12 (mean age 66.60 +/- 7.43, range 51-73) at the right body side. A group of 25 healthy, age-matched, subjects were also evaluated. To evoke the responses, trains of 10 electrical pulses were applied at different intensities and frequencies. Only intensities of stimulation > or = 5 times the sensory electrical threshold always assured bilateral plantar responses in all the examined subjects. Amplitude asymmetry between left and right responses was found only in the IPD patients (P < 0.05). The amplitude reduction corresponded to the motor affected side. No analogue latency variation was observed in any group. Independently from the peripheral or central origins of such phenomena, these findings suggest that simultaneous bilateral SSR amplitude evaluation could be useful, in early IPD patients, to demonstrate and to monitor the sympathetic cholinergic dysfunction, despite the lack of autonomic symptoms.
Assuntos
Doença de Parkinson/fisiopatologia , Pele/fisiopatologia , Sistema Nervoso Simpático/fisiopatologia , Idoso , Estimulação Elétrica , Eletromiografia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Tempo de Reação/fisiologia , Fatores de TempoAssuntos
Doenças do Gato/transmissão , Síndrome de Creutzfeldt-Jakob/transmissão , Doenças Priônicas/veterinária , Animais , Encéfalo/patologia , Doenças do Gato/patologia , Gatos , Síndrome de Creutzfeldt-Jakob/patologia , Feminino , Humanos , Itália , Masculino , Pessoa de Meia-Idade , Doenças Priônicas/patologia , Doenças Priônicas/transmissãoRESUMO
We present a patient with benign IgM-gamma anti-Sulfatide (SUL) whose neuropathy was transferred in newborn rabbits. The patient's clinico-pathological picture of anti-SUL-associated demyelinating neuropathy is reported. The monoclonal IgM antibodies prepared by Tatum's method, that retained their biological activity, were passively transferred to newborn rabbits. The passive transfer produced demyelinating nerve lesions very similar to the donor antibody neuropathy. In experimental lesions we observed the human IgM anti-SUL antibodies binding to Schmidt-Lanterman incisures and nodes of Ranvier. We postulate that the myelin-specific and complement-dependent lesions observed in the peripheral nerve support the potential demyelinating role of anti-SUL antibodies. Moreover, the pattern of the antibody binding to the perineuronal sheath of satellite cells in dorsal root ganglia strengthen the hypothesis that anti-SUL antibodies may have a pathogenetic role in this sensorimotor syndrome.
Assuntos
Anticorpos Monoclonais/imunologia , Imunoglobulina M/imunologia , Doenças do Sistema Nervoso Periférico/etiologia , Sulfoglicoesfingolipídeos/imunologia , Idoso , Animais , Humanos , Imunização Passiva , Masculino , Doenças do Sistema Nervoso Periférico/imunologia , Doenças do Sistema Nervoso Periférico/patologia , CoelhosRESUMO
Sera from 3 patients with breast or ovarian tumors and paraneoplastic cerebellar degeneration (PCD) contained anti-Purkinje cell antibodies (PCAbs) which also bind to other neurons on frozen sections of adult rodent brain. PCAbs tested on new-born rodent (rabbit, rat, mouse) brain tissue detected only oligodendrocyte-like cells (ODLC) in the white matter and allowed us to speculate on the nature of the antigenic structure in the neuronal cytoplasm. All these PCAbs appear histochemically identical and recognize antigens which belong to so-called "Yo" proteins.
Assuntos
Adenocarcinoma/imunologia , Doenças Autoimunes/imunologia , Sítios de Ligação de Anticorpos/imunologia , Neoplasias da Mama/imunologia , Doenças Cerebelares/imunologia , Degeneração Neural/imunologia , Proteínas do Tecido Nervoso , Neoplasias Ovarianas/imunologia , Adenocarcinoma/patologia , Adulto , Animais , Atrofia , Autoanticorpos/análise , Autoantígenos , Doenças Autoimunes/patologia , Encéfalo/imunologia , Encéfalo/patologia , Neoplasias da Mama/patologia , Doenças Cerebelares/patologia , Cerebelo/imunologia , Cerebelo/patologia , Proteínas de Ligação a DNA/imunologia , Epitopos/imunologia , Feminino , Humanos , Técnicas Imunoenzimáticas , Imageamento por Ressonância Magnética , Camundongos , Pessoa de Meia-Idade , Proteínas de Neoplasias/imunologia , Oligodendroglia/imunologia , Oligodendroglia/patologia , Neoplasias Ovarianas/patologia , Células de Purkinje/imunologia , Células de Purkinje/patologia , Coelhos , RatosRESUMO
We investigated the role of anti-myelin-associated glycoprotein (MAG) IgM and complement (C) in the pathogenesis of myelin alterations occurring in patients with anti-MAG-associated polyneuropathy. For this purpose, we separately studied the effects of anti-MAG antibodies and terminal C complex (TCC) after injection into the rabbit sciatic nerve. The two different local treatments produced identical ultrastructural abnormalities such as intramyelinic edema, myelin vesiculation and, in particular, separation of the major dense lines with the formation of widely spaced myelin, a peculiar feature encountered in human peripheral nerve disorders with circulating anti-myelin monoclonal IgM. In nerves treated with anti-MAG IgM ultrastructural myelin alterations were concurrent with activation of the rabbit's own C to the formation of TCC. Contrary to the immunological and ultrastructural findings obtained in C-sufficient animals, in C6-deficient rabbits injected with anti-MAG IgM no myelin alterations nor C completion were observed. This study identifies anti-MAG IgM as the mediator and the C as the effector of myelin changes observed in the present model and, for extension, in human neuropathies associated with anti-MAG IgM.
Assuntos
Anticorpos/farmacologia , Complexo de Ataque à Membrana do Sistema Complemento/farmacologia , Imunoglobulina M/imunologia , Imunoglobulina M/farmacologia , Proteínas da Mielina/imunologia , Bainha de Mielina/efeitos dos fármacos , Animais , Complemento C6/deficiência , Humanos , Injeções , Bainha de Mielina/ultraestrutura , Glicoproteína Associada a Mielina , Coelhos , Valores de Referência , Nervo Isquiático/efeitos dos fármacos , Nervo Isquiático/ultraestruturaRESUMO
Immunostaining of human, bovine and rodent unfixed nervous tissue sections was performed in order to characterize the structures recognized by anti-glycolipid antibodies. Four human sera from patients, two with M-IgM and motor neuron syndrome or motor neuropathy and two with motor neuropathy and polyclonal IgG antibody activity against gangliosides (GL; i.e. GM1, GD1b, GD1a), were utilized. Serum from a patient with sensory neuropathy and M-IgM immunoglobulins with antibody activity against sulfatide (SUL) was included in this series. This study shows that polyclonal and monoclonal anti-glycolipid antibodies give three different patterns of staining. The first is cholera toxin-like showing a more restricted neuronal pattern of staining. The second is peanut agglutinin-like, which includes the carbohydrate epitope shared by a group of glycoproteins in the gray and white matter. The third (anti-SUL) gives a preferential myelin staining. However, sera with anti-GM1 and anti-SUL antibodies recognize a number of closely situated determinants in the gray matter of the spinal cord and in the granule cells, while in peripheral nerves or in neuronal cells in culture their binding produces a different pattern (nodes of Ranvier for anti-GL; myelin for anti-SUL). These findings indicate that immunohistochemistry with anti-GL and anti-SUL antibodies may provide information regarding the glycolipid-bearing anatomical structures as target antigens and further substantiate the role of these molecules in the pathogenesis of autoimmune neurological disorders.
Assuntos
Anticorpos Monoclonais/imunologia , Glicolipídeos/análise , Sistema Nervoso/química , Esclerose Lateral Amiotrófica/metabolismo , Esclerose Lateral Amiotrófica/patologia , Animais , Autoantígenos/imunologia , Sequência de Carboidratos , Bovinos , Células Cultivadas , Epitopos/análise , Gangliosídeos/análise , Glicolipídeos/imunologia , Humanos , Soros Imunes , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Doença dos Neurônios Motores/metabolismo , Doença dos Neurônios Motores/patologia , Bainha de Mielina/química , Neurônios/química , Coelhos , Sulfoglicoesfingolipídeos/análiseRESUMO
We report the occurrence of a progressive neurological syndrome clinically characterized by subacute motor neuropathy in offspring of C6 deficient rabbits. On the basis of the pedigree analysis, the disease appears to be genetically transmitted, most probably with an autosomal recessive mode of inheritance. Pathological studies of affected animals revealed: transmitted, most probably with an autosomal recessive mode of inheritance. Pathological studies of affected animals revealed: 1) severe axonal degeneration in the sciatic nerve system involving mainly motor fibers; 2) occasional peripheral axonal enlargement closely associated with axonal degeneration; 3) presence of structured abnormal material in normal-size myelinated fibers of central nervous system (CNS) and peripheral nervous system (PNS); and 4) widespread occurrence of dystrophic axons and axonal spheroids in the gray matter of CNS. By ultrastructural examination, dystrophic axons are filled with tubulovesicular material, stalks of parallel membranes and dense bodies similar to what is described in human neuroaxonal dystrophies (NAD). The disease manifested by C6 deficient rabbits may represent an animal model of primary human NAD.
Assuntos
Axônios/ultraestrutura , Complemento C6/deficiência , Neurônios Motores/patologia , Degeneração Neural , Doenças do Sistema Nervoso/genética , Animais , Sistema Nervoso Central/patologia , Eletrofisiologia , Feminino , Masculino , Microscopia Eletrônica , Doenças do Sistema Nervoso/patologia , Doenças do Sistema Nervoso/fisiopatologia , Linhagem , Nervos Periféricos/patologia , CoelhosRESUMO
We investigated the role of complement in the pathogenesis of the demyelinating polyneuropathy that occurs in some patients with IgM monoclonal gammopathy. Seven patients with chronic sensorimotor polyneuropathy and IgM monoclonal gammopathy were examined. In six patients, the monoclonal protein recognized an epitope shared by myelin-associated glycoprotein and two peripheral-nerve glycolipids, whereas in one patient, IgM bound to an unidentified myelin antigen. Direct and indirect immunofluorescence and immunoperoxidase assays showed colocalization along the myelin sheaths of peripheral-nerve fibers of monoclonal protein with complement components C1q, C3d, and C5. In addition, terminal-complement complex that was not associated with S protein was detected in myelin sheaths. It appeared that alterations in myelin geometry caused by the separation of myelin lamellae corresponded to sites at which terminal-complement complex was deposited. We conclude that demyelination in polyneuropathy associated with IgM monoclonal gammopathy may be mediated by complement.
Assuntos
Proteínas do Sistema Complemento/imunologia , Doenças Desmielinizantes/imunologia , Bainha de Mielina/imunologia , Paraproteinemias/imunologia , Doenças do Sistema Nervoso Periférico/imunologia , Idoso , Complemento C1/análise , Complemento C3/análise , Complemento C5/análise , Doenças Desmielinizantes/etiologia , Doenças Desmielinizantes/patologia , Epitopos/imunologia , Feminino , Humanos , Imunoglobulina M/metabolismo , Masculino , Pessoa de Meia-Idade , Paraproteinemias/complicações , Paraproteinemias/patologia , Doenças do Sistema Nervoso Periférico/etiologia , Doenças do Sistema Nervoso Periférico/patologiaAssuntos
Dependência de Heroína/tratamento farmacológico , Síndrome de Abstinência Neonatal/tratamento farmacológico , Diazepam/administração & dosagem , Diazepam/uso terapêutico , Quimioterapia Combinada , Humanos , Recém-Nascido , Morfina/administração & dosagem , Morfina/uso terapêutico , Fenobarbital/administração & dosagem , Fenobarbital/uso terapêuticoRESUMO
We demonstrated that an IgM M-protein from a patient with motor neuron syndrome had antibody activity against gangliosides GM1, GD1b, and asialo GM1. Studies with a sugar-binding lectin suggested that the epitope in the patient's M-IgM involved the Gal(beta 1-3) GalNAc moiety. Immunohistological techniques demonstrated staining of axons in the lumbar roots, granular cells, and white matter in the cerebellum by the patient's M-IgM. We propose that, in this case, an autoimmune mechanism of motor neuron syndrome associated with a monoclonal protein is most likely.
Assuntos
Anticorpos Monoclonais/sangue , Autoanticorpos/sangue , Gangliosídeo G(M1)/imunologia , Gangliosídeos/imunologia , Neurônios Motores/imunologia , Doenças Neuromusculares/imunologia , Anticorpos Monoclonais/metabolismo , Autoanticorpos/metabolismo , Encéfalo/imunologia , Feminino , Humanos , Pessoa de Meia-Idade , Medula Espinal/imunologia , SíndromeRESUMO
We investigated two patients with the idiopathic hypereosinophilic syndrome and peripheral neuropathy. Clinical, EMG, and pathological findings were consistent with axonal polyneuropathy. Morphologic changes of the nerve biopsies suggested axonal damage secondary to increased endoneurial pressure from leakage of capillaries. We postulate that endothelial cell damage, followed by nerve edema, is the first step in the pathogenesis of peripheral neuropathy in these patients.
Assuntos
Eosinofilia/complicações , Doenças do Sistema Nervoso Periférico/etiologia , Biópsia , Capilares/patologia , Edema/etiologia , Edema/fisiopatologia , Eletrofisiologia , Eosinofilia/patologia , Eosinófilos/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Nervos Periféricos/irrigação sanguínea , Nervos Periféricos/patologia , Nervos Periféricos/fisiopatologia , Nervos Periféricos/ultraestrutura , Doenças do Sistema Nervoso Periférico/patologia , Doenças do Sistema Nervoso Periférico/fisiopatologiaAssuntos
Autoanticorpos/imunologia , Autoantígenos/imunologia , Doenças Autoimunes/fisiopatologia , Doenças do Sistema Nervoso/imunologia , Doenças do Sistema Nervoso Periférico/imunologia , Especificidade de Anticorpos , Reações Cruzadas , Gangliosídeos/imunologia , Glicoproteínas/imunologia , Humanos , Imunoglobulina M/imunologia , Proteínas da Mielina/imunologia , Bainha de Mielina/imunologia , Glicoproteína Associada a MielinaRESUMO
Neuropathological features of a case of ataxia-telangiectasia are reported. The main findings were the presence of Lewy bodies, cytoplasmic inclusions and axonal spheroids in the brainstem nuclei; pathological changes of spinal cord closely resembled those reported in the familial form of amyotrophic lateral sclerosis. In immunocytochemical studies, filamentous inclusions and axonal spheroids strongly reacted with monoclonal antibodies against neurofilament subunits. The results show that disorganization and accumulation of neurofilament proteins occur in ataxia-telangiectasia.
Assuntos
Ataxia Telangiectasia/patologia , Encéfalo/patologia , Citoesqueleto/patologia , Adulto , Anticorpos Monoclonais/imunologia , Ataxia Telangiectasia/imunologia , Encéfalo/ultraestrutura , Tronco Encefálico/patologia , Cerebelo/patologia , Córtex Cerebral/patologia , Grânulos Citoplasmáticos/imunologia , Citoesqueleto/imunologia , Humanos , Imuno-Histoquímica , Filamentos Intermediários/imunologia , Masculino , Medula Espinal/patologiaRESUMO
An adult with clinically and neuroradiologically occult diastematomyelia is described. The neuropathological study revealed an unusual duplication of the spinal cord not associated with osteocartilagineous anomalies. This anomaly probably occurs early in fetal life as an isolated lesion of the neural tube. The embryological mechanism is discussed.