RESUMO
Strongyloides venezuelensis has been used in different experimental studies, such as those aimed at the evaluation of diagnostic techniques for human strongyloidiasis, mainly the molecular diagnosis. In this study, three regions (genus, 18S and 28S targets) of Strongyloides ribosomal DNA were evaluated for the molecular diagnosis of experimental strongyloidiasis. Rats were infected subcutaneously with 400 or 4000 S. venezuelensis infective larvae (400iL3 and 4000iL3), and kept for 35 days. Fecal samples were collected daily to count eggs per gram of feces (EPG) and to perform the polymerase chain reaction (PCR). Egg count started on the 5th day post-infection (pi) and ended on days 33 and 34 pi, in 400iL3 and 4000iL3 groups, respectively. Based in EPG, fecal samples were selected from days 2, 5, 8, 11, 15, 23 and 35 pi for DNA extraction; PCR (genus, 18S and 28S); and sequencing. The PCR-28S products showed higher values of identity (95-100%) in the database with the Strongyloides sequences. Therefore, it is possible to reinforce the application of PCR-28S in the diagnosis of experimental and human strongyloidiasis.
Assuntos
DNA Ribossômico/genética , RNA Ribossômico 18S/genética , RNA Ribossômico 28S/genética , Strongyloides/genética , Estrongiloidíase/diagnóstico , Animais , DNA de Helmintos/genética , DNA de Helmintos/isolamento & purificação , DNA Ribossômico/isolamento & purificação , Fezes/parasitologia , Humanos , Larva/genética , Masculino , Contagem de Ovos de Parasitas , Reação em Cadeia da Polimerase/métodos , Reação em Cadeia da Polimerase/normas , Ratos , Ratos Wistar , Sensibilidade e Especificidade , Strongyloides/patogenicidadeRESUMO
The purpose of this study was to evaluate patient acceptance and perception of pain with regard to orthodontic mini-implants. The study was conducted on 58 individuals undergoing orthodontic treatment, who had orthodontic mini-implants placed as anchorage devices. Data were collected using a questionnaire containing 6 questions evaluating perception of pain during mini-implant placement and during use, difficulty with cleaning, unaesthetic appearance, difficulty with eating and benefits observed. Data were tabulated and analyzed using Fisher and Spearman's Correlation Coefficient tests. It was found that 94.8% of the patients reported that they would be willing to undergo treatment with mini-implants again. Of the negative aspects evaluated, the most significant was discomfort during placement, while the least significant was difficulty with eating. Patients' perception of aspects related to mini-implants was shown to be independent of the quantity of these devices placed. Although the patients evaluated some aspects of mini-implants negatively, the mean score for benefits observed was very high, indicating good patient satisfaction with treatment.
Assuntos
Implantes Dentários , Humanos , Procedimentos de Ancoragem OrtodônticaRESUMO
The purpose of this study was to evaluate patient acceptance and perception of pain with regard to orthodontic mini-implants.The study was conducted on 58 individuals undergoing orthodontic treatment, who had orthodontic mini-implants placed as anchorage devices. Data were collected using a questionnaire containing 6 questions evaluating perception of pain during mini-implant placement and during use, difficulty with cleaning, unaesthetic appearance, difficulty with eating and benefits observed. Data were tabulated and analyzed using Fisher and Spearmans Correlation Coefficient tests. It was found that 94.8% of the patients reported that they would be willing to undergo treatment with mini-implants again. Of the negative aspects evaluated, the most significant was discomfort during placement, while the least significant was difficulty with eating. Patients perception of aspects related to mini-implants was shown to be independent of the quantity ofthese devices placed. Although the patients evaluated some aspects of mini-implants negatively, the mean score for benefits observed was very high, indicating good patient satisfaction with treatment.
O objetivo do presente estudo foi avaliar a aceitabilidade e percepção dolorosa dos pacientes em relação aos mini-implantes ortodônticos. Este estudo foi realizado com 58 indivíduos em tratamento ortodôntico que tiveram a instalação de mini-implantes ortodônticos como recurso de ancoragem. Oinstrumento de coleta de dados foi um questionário contendo 8 perguntas que avaliaram a percepção dolorosa durante a instalação e uso dos mini-implantes, dificuldade de higienização,aspecto anti-estético, dificuldade de alimentação e os benefíciosobservados. Os dados foram tabulados e analisados pelos testes de Fisher e de Coeficiente de Correlação de Spearman. Os resultados demonstraram que 94,8% dos pacientes relataram que se submeteriam novamente ao tratamento com mini-implantes.Dos aspectos negativos avaliados, o mais significante foi o incômodo e dor durante a instalação, enquanto o menos significante foi dificuldade de alimentação. A percepção dos pacientes sobre os aspectos relacionados aos mini-implantesmostrou-se independente da quantidade desses dispositivos instalados. Conclui-se que apesar da avaliação dos mini-implantes pelos pacientes ter apresentado alguns aspectos negativos, o escore médio dos benefícios observados foi bastante alto, indicando boa satisfação dos pacientes com o tratamento.
