3D Printed In Vitro Dentin Model to Investigate Occlusive Agents against Tooth Sensitivity.

Tooth sensitivity is a painful and very common problem. Often stimulated by consuming hot, cold, sweet, or acidic foods, it is associated with exposed dentin microtubules that are open to dental pulp. One common treatment for tooth hypersensitivity is the application of occlusive particles to block dentin microtubules. The primary methodology currently used to test the penetration and occlusion of particles into dentin pores relies upon dentin discs cut from extracted bovine/human teeth. However, this method is limited due to low accessibility to the raw material. Thus, there is a need for an in vitro dentin model to characterize the effectiveness of occlusive agents. Three-dimensional printing technologies have emerged that make the printing of dentin-like structures possible. This study sought to develop and print a biomaterial ink that mimicked the natural composition and structure of dentin tubules. A formulation of type I collagen (Col), nanocrystalline hydroxyapatite (HAp), and alginate (Alg) was found to be suitable for the 3D printing of scaffolds. The performance of the 3D printed dentin model was compared to the natural dentin disk by image analysis via scanning electron microscopy (SEM), both pre- and post-treatment with occlusive microparticles, to evaluate the degree of dentinal tubule occlusion. The cytocompatibility of printed scaffolds was also confirmed in vitro. This is a promising biomaterial system for the 3D printing of dentin mimics.

A nondestructive contactless technique to assess the viscoelasticity of blood clots in real-time.

There is a need for reliable and quantitative real-time assessment of blood properties to study and treat a broad spectrum of disorders and cardiovascular diseases as well as to test the efficacy of hemostatic agents. In this study, the real-time changes in viscoelastic/rheological properties of bovine whole blood during coagulation induced by different concentrations of calcium chloride (CaCl2; 15, 25, 35 and 45 mM) was investigated. For this purpose, a novel, contactless technique was used to accurately measure the clotting characteristics under controlled and sterile conditions. It was demonstrated that, increasing the calcium concentration from low values (i.e., 15 and 25 mM), led to shorter reaction time; however, a further increase in calcium concentration (i.e., 35 and 45 mM) favored longer reaction times. Additionally, increasing the CaCl2 concentration resulted in higher shear storage modulus (i.e., stiffer clots). These results were also comparable to those generated by thromboelastrograph, a clinically established technique, as well as a conventional rheometer, which quantitatively verified the high correlation of the shear storage modulus data. In sum, the non-destructive testing technique used in this study is reproducible and sensitive in measuring clot formation kinetics, which could be applied to assess the efficacy of hemostatic agents, and may also contribute to better diagnosing relevant circulatory system diseases and conditions.

Bioactive glasses in wound healing: hope or hype?

Bioactive glasses have long been investigated in mineralized tissue regeneration, but recently their potential applications in soft tissue repair, and in particular wound healing, have demonstrated great promise. Commonly used glasses, such as the silicate-based Bioglass® 45S5 [(46.1)SiO2-(26.9)CaO-(24.4)Na2O-(2.6)P2O5 (mol%)] and borate-based 13-93B3 [(54)B2O3-(22)CaO-(6)Na2O-(8)K2O-(8)MgO-(2)P2O5 (mol%)] have been implicated in the stages of wound healing due to their ability to release ions that can stimulate processes, such as haemostasis, antibacterial efficacy, epithelial cell migration, angiogenesis, and fibroblastic cell proliferation, amongst others. More recently, a wound dressing composed of a borate-based glass received regulatory approval for use in the treatment of acute and chronic wounds. However, to date, there are no comprehensive reports on their specific mechanism of action in accelerating the wound healing processes. In this highlight, we will provide a brief overview of the wound healing stages, review the bioactive glass formulations that have been investigated for potential applications in wound healing and attempt to summarize the consensus on why these glasses may be successful in wound healing.