The genome sequence of the Golden-tailed Leafwalker, Xylota sylvarum (Linnaeus, 1758).

We present a genome assembly from an individual male Xylota sylvarum (the Golden-tailed Leafwalker; Arthropoda; Insecta; Diptera; Syrphidae). The genome sequence is 534.8 megabases in span. Most of the assembly is scaffolded into five chromosomal pseudomolecules, including the assembled X sex chromosome. The mitochondrial genome has also been assembled and is 16.0 kilobases in length. Gene annotation of this assembly on Ensembl identified 11,993 protein coding genes.

Evolution of miRNA-Binding Sites and Regulatory Networks in Cichlids.

The divergence of regulatory regions and gene regulatory network (GRN) rewiring is a key driver of cichlid phenotypic diversity. However, the contribution of miRNA-binding site turnover has yet to be linked to GRN evolution across cichlids. Here, we extend our previous studies by analyzing the selective constraints driving evolution of miRNA and transcription factor (TF)-binding sites of target genes, to infer instances of cichlid GRN rewiring associated with regulatory binding site turnover. Comparative analyses identified increased species-specific networks that are functionally associated to traits of cichlid phenotypic diversity. The evolutionary rewiring is associated with differential models of miRNA- and TF-binding site turnover, driven by a high proportion of fast-evolving polymorphic sites in adaptive trait genes compared with subsets of random genes. Positive selection acting upon discrete mutations in these regulatory regions is likely to be an important mechanism in rewiring GRNs in rapidly radiating cichlids. Regulatory variants of functionally associated miRNA- and TF-binding sites of visual opsin genes differentially segregate according to phylogeny and ecology of Lake Malawi species, identifying both rewired, for example, clade-specific and conserved network motifs of adaptive trait associated GRNs. Our approach revealed several novel candidate regulators, regulatory regions, and three-node motifs across cichlid genomes with previously reported associations to known adaptive evolutionary traits.

The genome sequence of the ferruginous bee-grabber, Sicus ferrugineus (Linnaeus, 1761).

We present a genome assembly from an individual male Sicus ferrugineus (the ferruginous bee-grabber; Arthropoda; Insecta; Diptera; Conopidae). The genome sequence is 312 megabases in span. The majority of the assembly (99.67%) is scaffolded into 5 chromosomal pseudomolecules, with the X and Y sex chromosomes assembled. The complete mitochondrial genome was also assembled and is 16.9 kilobases in length.

Meiosis and beyond - understanding the mechanistic and evolutionary processes shaping the germline genome.

The separation of germ cell populations from the soma is part of the evolutionary transition to multicellularity. Only genetic information present in the germ cells will be inherited by future generations, and any molecular processes affecting the germline genome are therefore likely to be passed on. Despite its prevalence across taxonomic kingdoms, we are only starting to understand details of the underlying micro-evolutionary processes occurring at the germline genome level. These include segregation, recombination, mutation and selection and can occur at any stage during germline differentiation and mitotic germline proliferation to meiosis and post-meiotic gamete maturation. Selection acting on germ cells at any stage from the diploid germ cell to the haploid gametes may cause significant deviations from Mendelian inheritance and may be more widespread than previously assumed. The mechanisms that affect and potentially alter the genomic sequence and allele frequencies in the germline are pivotal to our understanding of heritability. With the rise of new sequencing technologies, we are now able to address some of these unanswered questions. In this review, we comment on the most recent developments in this field and identify current gaps in our knowledge.

Evolution of regulatory networks associated with traits under selection in cichlids.

BACKGROUND: Seminal studies of vertebrate protein evolution speculated that gene regulatory changes can drive anatomical innovations. However, very little is known about gene regulatory network (GRN) evolution associated with phenotypic effect across ecologically diverse species. Here we use a novel approach for comparative GRN analysis in vertebrate species to study GRN evolution in representative species of the most striking examples of adaptive radiations, the East African cichlids. We previously demonstrated how the explosive phenotypic diversification of East African cichlids can be attributed to diverse molecular mechanisms, including accelerated regulatory sequence evolution and gene expression divergence. RESULTS: To investigate these mechanisms across species at a genome-wide scale, we develop a novel computational pipeline that predicts regulators for co-extant and ancestral co-expression modules along a phylogeny, and candidate regulatory regions associated with traits under selection in cichlids. As a case study, we apply our approach to a well-studied adaptive trait-the visual system-for which we report striking cases of network rewiring for visual opsin genes, identify discrete regulatory variants, and investigate their association with cichlid visual system evolution. In regulatory regions of visual opsin genes, in vitro assays confirm that transcription factor binding site mutations disrupt regulatory edges across species and segregate according to lake species phylogeny and ecology, suggesting GRN rewiring in radiating cichlids. CONCLUSIONS: Our approach reveals numerous novel potential candidate regulators and regulatory regions across cichlid genomes, including some novel and some previously reported associations to known adaptive evolutionary traits.

Mate choice and gene expression signatures associated with nutritional adaptation in the medfly (Ceratitis capitata).

Evolutionary responses to nutrition are key to understanding host shifts and the resulting potential for reproductive isolation. Experimental evolution has previously been used to describe the responses of the medfly (Ceratitis capitata) to larval diets with different nutritional properties. Within 30 generations this led to divergence in larval development time, egg to adult survival and adaptation in adult body size. Here we used mRNA-seq to identify differences in gene expression patterns in these same populations, using males from the 60th generation of nutritional selection. We validated differential expression by using qRT-PCR and found that genes linked to metabolism, oxidative phosphorylation and proteolysis were significantly over-represented among the differentially expressed genes. The results provide the first genome-wide survey of the putative mechanisms underpinning evolved responses to nutritional adaptation. In addition, we tested the hypothesis that nutritional adaptation can alter mating patterns. We found evidence for assortative mating by diet at generation 60, but not 90. Hence, the pattern was variable across generations and there was no evidence overall for any isolating mating divergence between the lines. Overall, the results provide insight into the mechanisms underpinning dietary adaptation and extend our knowledge of which traits represent core responses to nutritional selection.

Adaptation and conservation insights from the koala genome.

The koala, the only extant species of the marsupial family Phascolarctidae, is classified as 'vulnerable' due to habitat loss and widespread disease. We sequenced the koala genome, producing a complete and contiguous marsupial reference genome, including centromeres. We reveal that the koala's ability to detoxify eucalypt foliage may be due to expansions within a cytochrome P450 gene family, and its ability to smell, taste and moderate ingestion of plant secondary metabolites may be due to expansions in the vomeronasal and taste receptors. We characterized novel lactation proteins that protect young in the pouch and annotated immune genes important for response to chlamydial disease. Historical demography showed a substantial population crash coincident with the decline of Australian megafauna, while contemporary populations had biogeographic boundaries and increased inbreeding in populations affected by historic translocations. We identified genetically diverse populations that require habitat corridors and instituting of translocation programs to aid the koala's survival in the wild.