Design and characterization of Poly(glycerol sebacate)/Poly(3-hydroxybutyrate)/bioglass/curcumin nanocomposite scaffold for wound healing application.

Various applications have been developed for biopolymers, such as scaffolds for tissue engineering. Nanocomposite materials are considered promising for wound healing applications in many unique fields. New nanocomposite scaffold biopolymers were synthesized through the salt leaching technique. Curcumin and bioglass nanoparticles as antibacterial agents were added to Poly(glycerol sebacate)/Poly(3-hydroxybutyrate) nanocomposite scaffolds with different concentrations. Several properties were explored, including morphology, physicomechanical properties, contact angles, antibacterial efficacy, and in vitro studies. The morphology of nanocomposite scaffolds was characterized using SEM and EDX. Additionally, nanocomposite scaffolds Poly(glycerol sebacate)/Poly(3-hydroxybutyrate) showed a water contact angle of 79.8°. The hydrophilicity and water vapor transition rate significantly improved by adding bioglass nanoparticles which were 55° and 2182 g m-2 day-1 for Poly(glycerol sebacate)/Poly(3-hydroxybutyrate)/5 %Bioglass/3 %Curcumin. Samples containing 3 wt% Curcumin had the highest swelling ratio (347 ± 12 %) and the lowest water contact angle. Furthermore, NIH/3T3 fibroblast cells showed significant attachment and viability in in-vitro biocompatibility tests. Bioglass and Curcumin inhibited bacterial growth effectively. Additionally, an in-vitro cell viability, cell attachment, and in-vitro scratch wound healing assay demonstrated that the Poly(glycerol sebacate)/Poly(3-hydroxybutyrates)/5 % Bioglass/3 % Curcumin nanocomposite scaffold could promote wound healing.

Design and characterization of bio-elastomers containing biomaterials for tissue engineering application.

AIMS: The purpose of this research is to fabricate a new type of bio-elastomer based on Poly(glycerol-sebacate)-co-Poly(hydroxybutyrate) (PGS-co-PHB) with varying amounts of bioglass 45S5 (BG) nanoparticles (1, 3 and 5 wt%) through the green polycondensation polymerization for tissue engineering applications. MATERIALS AND METHODS: Fabricated composite films are characterized by FTIR, 1H NMR, SEM, EDX, contact angle, DMTA, biodegradability, and biocompatibility. The cell viability and morphology of L929 cells are investigated by indirect MTT assay and SEM analysis, and the antibacterial activity of composite film is determined by the disk diffusion method. Furthermore, the bioactivity of the composite film is measured by soaking in simulated body fluid (SBF), and XRD and SEM determined the formation of a hydroxyapatite (HA) layer. KEY FINDINGS: The hydrophilicity improved by adding BG nanoparticles, and the water contact angle was reduced to 63.46°. Furthermore, the average cell viability of composite film is about 94 %, and the SEM images show that L929 fibroblast cells are well spread on the surface of the composite film. BG has a significant influence on the antibacterial activity of composite film as PGS-co-PHB/5 %BG shows more antibacterial properties due to the higher amount of BG. SEM and XRD analyses confirmed the presence of crystalline HA on the surfaces of the composite film, indicating their potential for high bioactivity. SIGNIFICANCE: The results indicate that the antibacterial composite films are excellent supports for cell growth and proliferation and could be promising candidates for tissue engineering applications.