Water energy food nexus model: an integrated aqua-agriculture system to produce tilapia and sweet basil using desalinated water.

Under the framework of Water-Energy-Food Nexus, this study investigates the integration of solar-powered desalination with aquaculture and agriculture production systems to grow crops. Brackish water desalination is performed using reverse osmosis (RO), the permeate is directed to an aquaculture unit, and the fish effluent is used as irrigation water for crops. The experiment followed a split-plot design with three main variants: a full irrigation schedule for the basil plants with fish effluents as fertilizers, irrigation as fertigation with chemical fertilizers, and a mixed treatment of effluents and chemical fertilizers at 50% application each. Each treatment was further sprayed with or without foliar nutrient application. RO results gave a permeate recovery of 22%, and a salt rejection of 98.7%. In the aquaculture unit, tilapia harvest weight was 0.458 kg with a survival rate of 97.7% and harvest yield of 25 kg/ m3 of water. Effluent treatment exhibited the highest branching and plant height irrespective of the cut number, reaching 17.7 branches and 62-cm height. The effluent treatment under foliar application recorded the highest fresh and dry weights of basil with 14.7 ton/ha and 4.7 ton/ha, respectively. Effluent irrigation plus foliar application recorded basil yield of 5 kg/m3 effluent water. The dominant essential oil elements were linalool (55.5-61.6%), tau.-cadinol (5.3-8.3%), eucalyptol (5.4-7.7%), eugenol (2-6.8%), and (Z,E)-.alpha.-farnesene (3-5.2%). The study is among the very few integrated systems and, in particular, the current study is the first-time investigation of an integrated desalination, aquaculture, and agriculture production using renewable energy. Therefore, results suggest that integrating desalination, aquaculture, and agriculture could be a potential solution for the global water, energy, and food challenges.

A sandponics comparative study investigating different sand media based integrated aqua vegeculture systems using desalinated water.

This study investigated the utilization of fish effluents as irrigation water and nutrient sources to close the crop yield gap and increase Swiss chard productivity in a closed-loop sandponics system. The experiment was operated using desalinated water from a Reverse Osmosis plant. The study followed a completely randomized design with four variants, i.e., an aquaponic system (T1) and three sandponics systems; October (T2), Benu Suef (T3) and Fayoum (T4). Results indicated that T2 and T4 significantly recorded the highest plant heights in all cuts. The number of leaves per plant decreased with the increase in cut number. Leaf area and chlorophyll was significantly different between the treatments. T1 significantly had low biomass yields in cuts one and two, almost 40% less than T3 and T4. The various systems efficiently minimized water consumption ranging from 1.5 to 1.96 L/m2/day. The crop protein content ranged from 11.84 to 18.72 mg/100 g dry weight. Mineral composition in cut one was significantly higher compared to cuts two and three. The study recommends a novel technique for increasing crop production using fish effluents under sandponics systems while increasing water and fertilizer efficiency to close the crop yield gap.

Investigating the performance of hydroponic nutrient solutions as potential draw solutions for fertilizer drawn forward osmosis.

This research project aims at investigating the performance of hydroponic nutrient solutions as draw solutions for desalination using the fertilizer drawn forward osmosis (FDFO) process. Six different lettuce and leafy greens hydroponic nutrient stock solutions were prepared according to the literature and used in this study and tested on a bench-scale forward osmosis unit as draw solutions for the process. The feed solution for the process was deionized water mixed with NaCl in different concentrations, to represent different salinities of brackish groundwater. The draw efficiency of each solution was measured based on water flux, specific reverse solute flux, water recovery, and salt rejection. It was concluded that of the six tested nutrient solutions, the "Resh Florida, California" solution is the recommended solution to be used as draw solution for fertilizer drawn forward osmosis, due to its high performance in terms of water recovery (15.75%), flux (11 L/m2/h), salt rejection (92%), and SRSF (highest recorded SRSF for a specific ion (SO42-) was 7.3 g/L), as well as its low cost, relative to the other highly performing draw solution "Chekli" ($1.07/L vs. $3.73/L).

Investigating sustainable management of desalination brine through concentration using forward osmosis.

A fertilizer drawn forward osmosis (FDFO) process was tested for the concentration of synthetic brine using an industrial-grade fertilizer ammonium sulfate (NH4)2SO4 as the draw solution (DS), NaCl-based synthetic brine as the feed solution (FS), and a commercial forward osmosis (FO) membrane. A bench-scale investigation and a pilot-scale investigation were carried out. By using the highest possible concentration of the DS with a fixed concentration of the FS, the brine generated by reverse osmosis (RO) desalination plants was simulated. The aim of this investigation, performed in batch mode, was to assess the feasibility of using the FDFO process with the tested DS to concentrate the brine by extracting water to dilute the DS. While the main aim of the investigated process was achieving the maximum possible volume reduction of the brine, the resulting DS was further diluted to reduce the nutrients' concentration in the diluted DS to the acceptable levels producing fertilized water that can be used for fertigation. The investigation showed that the proposed process using the tested fertilizer resulted in an average water flux of 8.01 l/h/m2, and a volume reduction of the brine of around 12%.

The pro-apoptotic effect of a Terpene-rich Annona cherimola leaf extract on leukemic cell lines.

BACKGROUND: The edible fruit Annona cherimola has previously shown many nutritional and medicinal properties. The current study evaluates the anti-cancer and anti-proliferative properties of Annona cherimola ethanolic leaf extract (AELE) on Acute Myeloid Leukemia (AML) cell lines cultured in vitro (Monomac-1 and KG-1). METHODS: The anti-proliferative effect of A. cherimola ethanolic leaf extract was evaluated via cell viability assay. Its pro-apoptotic effect was assessed through Cell Death ELISA and dual Annexin V/PI staining. To further investigate the molecular mechanism by which the extract promoted apoptosis and inhibited the proliferation of the AML cells used, apoptotic protein expression was determined through western blots. Extract composition was elucidated by Gas Chromatography-Mass Spectrometry (GC-MS). RESULTS: Our results showed that the treatment with A. cherimola ethanolic leaf extract exhibited an inhibitory effect on the proliferation of both cancer cell lines used in a dose- and time-dependent manner, with no toxic effects on normal mononuclear cells (MNCs) isolated from human bone marrow. This effect was mediated by DNA fragmentation and apoptosis, as revealed by Cell Death ELISA and dual Annexin V/PI staining. Western blot analysis revealed a Bax/Bcl2 dependent mechanism of apoptosis, as well as PARP cleavage, confirming the apoptotic results observed previously. These effects may be attributed to the presence of terpenes which constitute a large component of the leafy extract, as revealed via GC-MS. CONCLUSION: All the data presented in our study show that the terpene-rich A. cherimola ethanolic leaf extract exhibits an anti-proliferative and pro-apoptotic effect on the AML cell lines used.

Annona cherimola Seed Extract Activates Extrinsic and Intrinsic Apoptotic Pathways in Leukemic Cells.

Annona cherimola Mill is a large green fruit with black seeds widely known to possess toxic properties due to the presence of Annonaceous acetogenins. The present study investigates the anti-cancer properties of an Annona cherimola Mill ethanolic seed extract on Acute Myeloid Leukemia (AML) cell lines in vitro and elucidates the underlying cellular mechanism. The anti-proliferative effects of the extract on various AML cell lines and normal mesenchymal cells (MSCs) were assessed using WST-1 viability reagent. The pro-apoptotic effect of the extract was evaluated using Annexin V/PI staining and Cell Death ELISA. The underlying mechanism was deciphered by analyzing the expression of various proteins using western blots. Treatment with an A. cherimola seed ethanolic extract promotes a dose- and time-dependent inhibition of the proliferation of various AML cell lines, but not MSCs. Positive Annexin V staining, as well as DNA fragmentation, confirm an increase in apoptotic cell death by upregulating the expression of pro-apoptotic proteins which control both intrinsic and extrinsic pathways of apoptosis. GC/MS analysis revealed the presence of phytosterols, in addition to other bioactive compounds. In conclusion, Annona cherimola Mill seed extract, previously known to possess a potent toxic activity, induces apoptosis in AML cell lines by the activation of both the extrinsic and the intrinsic pathways.

Aquaponics: a sustainable alternative to conventional agriculture in Egypt - a pilot scale investigation.

Conventional agriculture has been widely used to overcome food scarcity in Egypt, but in turn created new environmental, social, and economic problems. Aquaponics has a huge potential as a sustainable solution since it demonstrates promising results for urban food development while providing new entrepreneurship and start-up opportunities. Although it presents a big opportunity especially for developing countries like Egypt, it still requires further studies and researches to fully overcome foreseen challenges. This study discusses the possibility of implementing aquaponics as an alternative to conventional agriculture in Egypt through a comparison between aquaponics and conventional agriculture. The first section of this investigation analyzes the economic feasibility of aquaponics in terms of capital and operational expenditure costs. Then, an experimental comparative study is carried out examining two pilot-scale aquaponics systems (Deep water Culture system and Integrated Aqua-Vegaculture system), where crop quality (vitamins, heavy metals, and pesticides residues) of the two systems has been compared among each other, as well as to that of the commercially available organic food available in supermarkets. Results indicated that both systems produce high-quality safe organic food. The economic feasibility analysis indicated that Integrated Aqua-Vegaculture System is producing more crops with a wider variety of almost 20% less capital expenditure and operational expenditure costs. Fish production for both systems was close, yet slightly higher in Deep Water Culture. Although, on the short term, aquaponics entails relatively high capital and operational expenditure costs compared with conventional agriculture; yet, on the long term, it is more profitable, while saving up to 85% of the water wasted by conventional farming techniques. Finally, the study discusses the main limitations, drawbacks, and future of aquaponics in Egypt where public awareness and a new mindset are needed to highlight the link between agriculture and sustainability.