[The outlook for the creation of a bank of cryopreserved cord blood for clinical use]. / Perspektiva sozdaniia banka kriokonservirovannoi kordovoi krovi dlia klinicheskogo primeneniia.

Appraisal of morphological and functional features of cord blood cells involving identification of precursor cells marked with specific monoclonal antibodies makes it possible to regard cord blood as biologically valuable hemopoietic tissue with a high repopulation potential. Studies aimed at developing optimal conditions of cryopreservation allow the stocks of biologically valuable tissue to be built up.

[The correction of disorders of the rheological properties of the blood in pancreatitis]. / Korrektsiia narushenii reologicheskikh svoistv krovi pri pankreatite.

In 20 patients operated on for acute pancreatitis, the blood plasma viscosity, aggregative characteristics of erythrocytes and thrombocytes were studied after the operation. Effectiveness of inclusion into the complex of treatment of corrigative therapy, therapeutic plasmapheresis and plasmosorption is shown.

[The status of intracellular metabolism during intensive therapy of severe surgical endotoxicosis]. / Sostoianie vnutrikletochnogo metabolizma pri intensivnoi terapii tiazhelogo khirurgicheskogo éndotoksikoza.

Introduction of separation plasmapheresis and plasmasorption into intensive therapy of 60 patients with surgical endotoxemia ensured marked detoxication, which manifested in earlier improvement of the clinical symptoms and normalization of intracellular metabolism in cellular elements of the peripheral blood. The data obtained make it possible to recommend the use of the above treatment procedures for elimination of profound intracellular metabolic disturbances in the postoperative period.

[Specifics of wound healing in patients with extremely severe burns as effected by various methods of treatment]. / Osobennosti techeniia ranevogo protsessa u bol'nykh s ozhogami kraine tiazheloi stepeni pod vliianiem razlichnykh metodov lecheniia.

Peculiarities of the course of a wound process in 52 patients ranging in age from 16 to 60 years with the extremely severe burns were studied. Suppression of the vascular reaction and deceleration of the cellular reactions of the extravascular phases of inflammation, alteration of protease-antiprotease activity at the region of injury in dynamics of a wound process were revealed. Antiinflammatory action of the combined methods of general and local sorptive detoxication were substantiated. Certain mechanisms of increase in anti-infective resistance of the organism of the sufferers were elucidated.

[Effect of sorption detoxification in severe burns on the development of cell-mediated reactions in the vascular and extravascular phases of inflammation]. / Vliianie sorbtsionnoi detoksikatsii pri tiazhelykh ozhogakh na razvitie kletochno-oposredovannykh reaktsii v sosudistoi i vnesosudistoi fazakh vospaleniia.

In combined use of general and local sorption detoxication in 30 patients with severe burns, the pronounced antiinflammatory action is caused by redistribution of neutrophilic granulocytes with different activity of myeloperoxidase systems, decrease in lesion of a vascular wall and development of the perfect cellular reactions into extravascular phase of inflammation. The area of deep burn wounds reduced 2.8-fold when compared with that in patients, in whom no sorption methods were used.

[The morphofunctional characteristics of lymphoma NK/LY cells treated with concanavalin A and Vibrio cholerae neuraminidase]. / Morfofunktsional'ne osobennosti kletok limfomy NK/LY, obrabotannykh konkanavalinom A i neiraminidazoi kholernykh vibrionov.

Mouse NK/LY lymphoma cells were modified by Con A or Vibrio cholerae neuraminidase (VCN). Severe damage of the modified cells was not revealed by either supravital staining or light microscopy. Ultrastructural signs of metabolic lowering in the Con-A-treated cells were determined. Some increase in electrophoretic mobility of Con-A-modified cells was found, along with its decrease in VCN-modified cells. The decrease in the pool of proliferating cells was determined in both the types of modification, being more evident in the Con-A-modified material. A marked tumorigenic decrease of Con-A-modified cells was fixed.

[The effect of combined methods of detoxication on the development of inflammatory reactions in severe burns]. / Vliianie sochetannykh metodov dezintoksikatsii na razvitie vospalitel'noi reaktsii pri tiazhelykh ozhogakh.

The development of inflammatory reaction in 106 patients with severe and extremely severe burns in combined use in the complex treatment of extracorporeal hemosorption, enterosorption, applicative sorption by the powdery PMS-Zn sorbent, passive immunotherapy was studied. The modulative effect of the methods of general and local sorptive detoxication on the development of inflammatory reaction of the area of thermal trauma with reduction of damaging effect of proteolytic enzymes and formation of cellular reaction directed at protection from microorganisms and beginning of the proliferative stage of reparative regeneration is shown. Lethality in severe burns was 17.7%, in extremely severe--50.8%.

[Ultrastructure of bone marrow stroma and endosteum in children with acute leukemia]. / Ul'trastruktura stromy kostnogo mozga i éndosta pri ostrom leikoze u detei.

Ultrastructural peculiarities of stromal elements from bone marrow and endosteum in 28 children with acute leukaemia during clinical and hematological exacerbations are presented. Investigations are conducted before chemotherapy. Changes in ultrastructure of all stromal components are revealed. Of special significance are the data on variations of reticular cells and rough desorganization of fibrillar structures. Disturbances in intercellular contacts between hemopoietic cells and stromal elements are detected.

[Modulating effect of immunotherapy with modified tumor cells in transplantable lymphoma]. / Moduliruiushchii éffekt immunoterapii modifitsirovannymi opukholevymi kletkami pri perevivnoi limfome.

Vibrio cholerici neuraminidase was studied on CBA mice for its effect on the strain NK/Ly lymphoid cells oncogenicity in intraabdominal transplantation. The 30% decrease in transplantability of cells treated by the enzyme in the dose of 50 U/ml was established. The modulating effect of specific active immunotherapy by modified cells in lymphoma was also studied. A pronounced stimulation of immunity T-cell links and proliferation in immunocompetent organs, an increase in the number of large granular lymphocytes in blood, specific antibody formation suppression were established. The problem of neuraminidase nonspecific effect on the tumour cells is under discussion.

[Embryonal hematopoiesis in the intact human liver and in its organ culture]. / Embrional'noe krovetvorenie v intaktnoi pecheni cheloveka i v ee organotipicheskoi kul'ture.

Hepatic tissues in 58 human embryos, 5-12 week-old have been investigated. At that time hemopoiesis is mainly presented by erythropoiesis. Ultrastructure of the hepatic parenchymal cells and that of the hemopoietic cellular elements have been studied. The cultural system used makes it possible to maintain hemopoiesis in the explants for 3.5 weeks. Under the conditions of cultivation in the organic culture, certain changes in architectonics of the explants are observed. As the time of the cultivation increases, the ultrastructure of the hepatic parenchymal cells, which make the hemopoiesis-inducing microenvironment, undergo further changes. A gradual substitution of erythropoiesis for granulomonocytopoiesis takes place.

[Light and electron microscopic data on the characteristics of the utiliztion of amikin and aminozol, preparations for parenteral feeding]. / Osobennosti utilizatsii preparatov dlia parenteral'nogo pitaniia amikina i aminozola po dannym svetovoi i élektronnoi mikroskopii.

It has been extablished that in the course of protein starvation in dogs, parenteral administration of amikin or aminozol normalizes, to a greater of less extent, the disturbed metabolism of proteins, nucleoproteins, glycogen and lipids. Electron microscopy has shown that the ingredients of the preparations enter the cells at an equal intensity from the microcirculatory bed by means of active transendothelial transport. In the course of utilization of the substances supplied to the cells, no marked changes were seen in protein synthesis or in its participation in intracellular reparative regeneration. There occurs a pronounced cytosegresomal response to unutilized substances accumulated by hepatocytes. The clinical efficacy of the preparations used is related to the processes of gluconeognesis of amino acids supplied as a result of which energy formation does not require structural or endogenously formed protein.