Effect of periodontal debridement plus systemic azithromycin in subjects with stage III periodontitis: a randomized controlled clinical trial / Efecto del desbridamiento periodontal más azitromicina sistémica en sujetos con periodontitis estadio III: un ensayo clínico controlado aleatorio

Aim: To evaluate the effect of the systemic administration of azi-thromycin (AZM) as an adjunct to non-surgical periodontal therapy (NSPT) on the clinical and microbiological variables of patients with periodontitis. Material and Methods: Eighteen volunteers received NSPT combined with placebo or AZM (500 mg/day) for 3 days (n=9/group). They were monitored clinically for probing pocket depth (PPD), clinical attachment level (CAL), O'Leary index (OI), bleeding on probing (BoP) at baseline and during the first, third and sixth month and microbiologically, at baseline and at 3 and 6 months after therapy, by conventional polymerase chain reaction tests. Results: Fourteen patients completed the study (n=7/group). Differences statistically significant were observed among both groups. The experimental group presented: A PPD mean (p=0.04) significantly lower and PPD reduction (p=0.02), at 6-months post NSPT. Regarding changes (∆), at the third month post NSPT, there was a significant increase in the number of shallow sites (p<0.001) and a decrease in the intermediate sites (p<0.001). In addition, a significant decrease in the mean number of deep sites (p=0.04) was detected at 6 months post treatment. There was also a significant decrease in periodontal index BoP at 1 (p=0.01), 3 (p<0.001) and 6 (p=0.01) months and OI at 3- and 6-months (p<0.001), post treatment. Regarding the presence of periodontal pathogens, no significant differences were observed, intra and inter groups. Conclusion: AZM as an adjuvant to NSPT provides additional beneficial effects for PPD and BoP compared to NSPT alone.

Objetivo: Evaluar el efecto de la administración sistémica de azitromicina (AZM) como coadyuvante de la terapia periodontal no quirúrgica (TPNQ) en las variables clínicas y microbiológicas de pacientes con periodontitis. Material y Métodos: Dieciocho voluntarios recibieron TPNQ combinado con placebo o AZM (500 mg/día) durante 3 días (n=9/grupo). Fueron monitoreados clínicamente para determinar Profundidad de Sondaje del Saco (PSS), Nivel de Inserción Clínica (NIC), Índice de O'Leary (IO), Sangrado al sondaje (SS) al inicio y durante el primer, tercer y sexto mes y microbiológicamente, al inicio y a los 3 y 6 meses después de la terapia, mediante la reacción en cadena de la polimerasa convencional. Resultados: Catorce pacientes completaron el estudio (n=7/grupo). Se observaron diferencias estadísticamente significativas entre ambos grupos. El grupo experimental presentó una media de PSS significativamente menor (p=0,04) y una reducción de PSS (p=0,02), a los 6 meses post TPNQ. En cuanto al delta (∆) pre y post tratamiento, al tercer mes post TPNQ, hubo un aumento significativo en el número de sitios poco profundos (p<0.001) y una disminución en los sitios intermedios (p<0.001). Además, se detectó una disminución significativa en la media de los sitios profundos (p=0.04) a los 6 meses post tratamiento. También hubo una disminución significativa en el índice SS al primer (p=0.01), tercer (p<0. 0 01) y sexto mes (p=0.01) post TPNQ y del IO al tercer y sexto mes (p<0.001), post tratamiento. En cuanto a la presencia de patógenos periodontales, no se observaron diferencias significativas tanto intra como ínter grupos. Conclusión: AZM como adyuvante a TPNQ proporciona efectos benéficos adicionales en la PSS y SS en comparación a TPNQ solo.

Asociación entre osteoporosis y periodontitis crónica / Association between osteoporosis and chronic periodontitis

La periodontitis crónica es una enfermedad inmunoinflamatoria causada por bacterias. Debido a los múltiples factores involucrados en la patogenia periodontal existen una serie de estudios sobre su probable asociación con diversas condiciones sistémicas, entre ellas osteoporosis. Esta es una enfermedad sistémica progresiva, caracterizada por una pérdida de masa ósea y deterioro de su microarquitectura, comprometiendo el hueso trabecular y cortical. El objetivo de este estudio fue identificar si existe asociación entre osteoporosis y periodontitis crónica, de acuerdo a la literatura especializada publicada hasta la fecha. Para tal efecto se realizó una búsqueda de la literatura en la base de datos Medline mediante su buscador PubMed. Se utilizó la siguiente estrategia de búsqueda: osteoporosis (Mesh) OR osteoporosis (TI) AND periodontal disease AND odds ratio OR osteoporosis (Mesh) OR osteoporosis (TI) AND periodontal disease AND association, seleccionando estudios en humanos, publicados en los últimos 10 años. Finalmente, 6 artículos fueron analizados. Estudios recientes entregan una fuerte evidencia de asociación entre osteoporosis y pérdida de inserción clínica en humanos. En función de estos resultados se puede concluir que existe una relación de riesgo entre la disminución de la densidad ósea esqueletal y la altura de la cresta ósea alveolar, sin embargo aún no es posible determinar una relación causal. Hasta la fecha esta asociación solo puede confirmarse en mujeres.

Chronic periodontitis is an immuno-inflammatory diseases caused by bacteria. Due to the multiple factors involved in periodontal pathogenesis, there have been a number of studies on the probable association with various systemic conditions, including osteoporosis. This is a progressive systemic disease characterized by loss of bone mass and microarchitectural deterioration, compromising trabecular and cortical bone. The aim of this study was to identify the association between osteoporosis and chronic periodontitis, according to the specialized literature published to date. To this end, a literature was performed in MEDLINE database using its PUBMED browser. The following search strategy was used: Osteoporosis (Mesh) OR osteoporosis (IT) AND periodontal disease odds ratio OR Osteoporosis (Mesh) OR osteoporosis (IT) AND periodontal disease association, selecting human studies, published in the last 10 years. Finally, 6 items were analyzed. Recent studies provide strong evidence of an association between osteoporosis and clinical attachment loss in humans. Based on these results, it can be concluded that there is a relationship between decreased risk of skeletal bone density and height of the alveolar bone crest, however it is not yet possible to determine a causal relationship. So far this association can only be confirmed in women.

Correlación entre biotipo gingival, ancho y grosor de encía adherida en zona estética del maxilar superior / Correlation between gingival biotype, width and thickness of attached gingiva in an aesthetic of the upper jaw

Actualmente, para realizar distintos procedimientos odontológicos se hace necesaria una correcta evaluación del biotipo periodontal utilizando las herramientas adecuadas que nos permitan medir de manera certera su grosor. El objetivo de esta investigación fue evaluar distintos métodos diagnósticos, correlacionándolos con la medición directa de ancho y grosor de encía adherida en la zona anterosuperior del maxilar. Se reclutaron 30 pacientes periodontalmente sanos, y en los dientes 1.1, 1.2 y 1.3 se realizó: identificación del biotipo según parámetros visuales, medición directa del ancho, grosor de encía adherida y transparencia de la sonda periodontal como método indirecto. Mediante la transparencia de la sonda, el biotipo grueso fue el más prevalente (53,3 por ciento), observándose más en hombres (62,5 por ciento) versus mujeres (37,50 por ciento). Según parámetros visuales, el biotipo fino fue más prevalente (56,7 por ciento) que el grueso (43,3 por ciento), y al comparar ambos métodos no existieron diferencias significativas. Se observó un ancho promedio de la encía adherida de 2,79 mm y un grosor de 1,06 mm, presentándose valores más elevados en el biotipo grueso (ancho 2,94 mm y grosor 1,10 mm) versus el fino (ancho 2,67 mm y grosor 1,01 mm). Según los resultados obtenidos podemos concluir que tanto el diagnóstico visual como la transparencia de la sonda son válidos para identificar el biotipo gingival. Sin embargo, la inspección visual mostró menor reproducibilidad y mayor porcentaje de error al definir biotipo fino.

To perform different dental procedures it is currently necessary to make a correct assessment of periodontal biotype, using the right tools that allow its thickness to be accurately measured. The objective of this study was to evaluate different diagnostic methods and correlating them with direct measurement of width and thickness of attached gingiva in the front upper jaw area. The study included 30 periodontally healthy patients, in whom biotype identification was performed on teeth 1.1, 1.2 and 1.3, using visual parameters, direct measurement of the width and attached gingiva thickness, and transparency of the periodontal probe as an indirect method. According to the transparency of the probe, the thick biotype was the most frequent (53.3 percent) was observed more in men (62.5 percent) than women (37.50 percent). According to the visual parameters, thin biotype was more prevalent (56.7 percent) than the thick biotype (43.3 percent). There were no significant differences on comparing the 2 methods. The mean width of attached gingiva was 2.79 mm and a thickness of 1.06 mm, being greater in the thick biotype (width 2.94 mm and thickness 1.10) than in the thin biotype (width 2.67 mm and thickness 1.01 mm). According to the results, it can be concluded that both the visual diagnosis and transparency of the probe are valid to identify the gingival biotype. However, a lower reproducibility by visual inspection was observed, and the thin biotype showed a higher error rate.

Interferon-γ, interleukins-6 and -4, and factor XIII-A as indirect markers of the classical and alternative macrophage activation pathways in chronic periodontitis.

BACKGROUND: Macrophages account for 5% to 30% of the inflammatory infiltrate in periodontitis and are activated by the classic and alternative pathways. These pathways are identified by indirect markers, among which interferon (IFN)-γ and interleukin-6 (IL)-6 of the classic pathway and IL-4 of the alternative pathway have been studied widely. Recently, factor XIII-A (FXIII-A) was reported to be a good marker of alternative pathway activation. The aim of this study is to determine the macrophage activation pathways involved in chronic periodontitis (CP) by the detection of the indirect markers IFN-γ, IL-6, FXIII-A, and IL-4. METHODS: Biopsies were taken from patients with CP (n = 10) and healthy individuals (n = 10) for analysis of IFN-γ, IL-6, IL-4, and FXIII-A by Western blot (WB), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). The same biopsies of healthy and diseased gingival tissue were used, and the expressions of these markers were compared between healthy individuals and those with CP. RESULTS: The presence of macrophages was detected by CD68+ immunohistochemistry and their IFN-γ, IL-6, IL-4, and FXIII-A markers by WB, IHC, and ELISA in all samples of healthy and diseased tissue. IL-6, IL-4, and FXIII-A were significantly higher in patients with CP, whereas FXIII-A was higher in healthy individuals. CONCLUSION: The presence of IFN-γ, IL-6, IL-4, and FXIII-A in healthy individuals and in patients with CP suggests that macrophages may be activated by both classic and alternative pathways in health and in periodontal disease.