RESUMO
Due to the emergence of the SARS-CoV-2 B.1.1.7 (Alpha) variant in the UK in 2020 and its risk of increased transmission, the Ministry of Health in Tunisia implemented a sequencing surveillance strategy for SARS-CoV-2. The aim of this study was to analyze SARS-CoV-2 genomic surveillance data in Tunisia (January 2021-February 2022) and to assess the implementation of the sequencing strategy for SARS-CoV-2 in accordance with national recommendations and the guidance for SARS-CoV-2 genomic surveillance for public health goals. A descriptive study of all sequenced RT-PCR samples sequenced (January 2021-February2022). An internal audit was also done to assess the compliance against standards covering national recommendations and the Guidance for SARS-CoV-2 genomic surveillance for public health goals. A total of 12 simple or composite requirements related to the following areas were included in the audit standards: sampling (one requirements); data collection/analysis (six requirements); partnership (one requirement); and ethical considerations (one requirement). A total of 4819 samples were sent to laboratories and 4278 samples were sequenced. A total of 3648 samples were classified. Positive variants of concern (VOC) samples were 80.92%, differentiated as follows: Alpha, 40.24%; Beta, 0.24%; Gamma, 0.03%; Delta, 45.26%; and Omicron, 14.19%. Three principal phases of VOCs per ISO-week were shown: Alpha 3/2021-25/2021; Delta 26/2021-2/2022; and Omicron 3/2022-6/2022. Levels of compliance were identified; from a total of 12 requirements, 7 were considered as "not met", 4 as "partially met", and 1 as "fully met" but including not totally achieved objectives. In conclusion, the internal audit of the national SARS-CoV-2 sequencing strategy revealed an overall "not met" level of compliance. The results offered a trigger to collaborate with all stakeholders to develop a surveillance strategy for early detection and response to outbreaks caused by VOCs.
RESUMO
INTRODUCTION: The occupational infectious risk in the health care environment is potentially ubiquitous. Several infectious agents are transmitted to healthcare professionals, especially by blood and body fluids. AIM: To describe the knowledge; attitudes and practices related to blood exposure accidents (BEA) among operating room nurses and to determine their associated factors. METHODS: A descriptive correlational study was conducted from February 24 to April 10, 2022, among nurses of the operating rooms in the two university hospitals of Sousse using an auto administrated questionnaire inspired from the literature. RESULTS: Our population was composed of 133 nurses. The average age was 38.79 ±7.75 years and the average length of work was 12.02 ±7.8 years. Overall, the respondents had moderate knowledge about BEA. Indeed, 17.3% did not know the meaning of the acronym BEA. Concerning the attitudes, almost (63.1%) had been the victim of at least one BEA. The most frequent types of BEA were pricks (81%), cuts (37%) and splashes (33.3%). The mechanisms of occurrence were mainly recapping (91.7%) and disposal of health care waste (74.4%). Only 48.1% of respondents had received training on BEA. The systematic use of gloves for health care procedures was unsatisfactory (66.2%). According to the studied practices, in case of injured skin, (82.7%) of the respondents wore gloves if they had any skin lesions. Almost all respondents (90.2%) were vaccinated against hepatitis B. Women had better knowledge about the meaning of the acronym BEA (p=0.011). The comparison of practices showed that recapping dirty needles after use was significantly higher among females than males (p=0.011). CONCLUSION: The solid knowledge about the BEA and systematic application of standard precautions in health care settings must be concretely manifested in the daily practices of health professionals.
