Gluten-free Nan-e-Fasaee: Formulation optimization on the basis of quinoa flour and inulin.

Diversification of gluten-free (GF) bakery products is considerably important, as those who suffer from gluten intolerance should follow a GF diet their whole life. Regarding this study, it was aimed at optimizing the formulation of a quinoa-based GF traditional bakery product, i.e. Nan-e-Fasaee using inulin as a bifunctional agent (both a prebiotic compound and a structure-forming agent). Otherwise, its potential role as a fat and sugar replacer was also assessed. For this purpose, short (S)- and long (L)-chain inulin were used as sugar and fat replacers, respectively, at 0%-50% w/w in quinoa flour (QF)-based GF Nan-e-Fasaee and optimization was done based on rheological, textural, and sensory analysis. Results indicated that QF diet provided the batter with the dominance of elastic modulus and increased hardness (i.e. 5170.0 ± 22.50 g in the presence of QF compared to 1477.0 ± 20.81 g in wheat-based ones). Inulin inclusion reduced the hardness, as the lowest was observed at S-inulin substitution levels of 40% and 50% w/w, with values equal to 2422.0 ± 20.81 and 2431.0 ± 35.57 g, respectively (the most similar ones to control sample). The interference of S-inulin with the non-gelatinized starch structure is supposed to decrease the hardness. The highest score in texture was also perceived at F6 and F13, with values equal to 8.00 ± 0.10 and 7.97 ± 0.05, respectively. Using S- and L-inulin in combination is found to improve the textural characteristics due to preventing the competitive role of sugar in water absorption in formulations containing L-inulin. Regarding optimization of quinoa-based GF Nan-e-Fasaee with reduced sugar and fat levels using inulin, it is found to be feasible.

The concentration of radioisotopes (Potassium-40, Polonium-210, Radium-226, and Thorium-230) in fillet tissue carp fishes: A systematic review and probabilistic exposure assessment.

Chemical contamination of seafood has become a global health concern. Carp fish is one of the most widely consumed globally, and several studies have been conducted on the contamination of carp fish with radioisotopes. In the current study, a meta-analysis and probabilistic exposure assessment regarding the Potassium-40 (40K), Polonium-210 (210Po), Radium-226 (226Ra), and Thorium-230 (230Th) in the fillet tissue of carp fish were performed. In this regard, Scopus and PubMed were screened to retrieve the associated citations with on the concentration of radioisotopes in the fillet tissue of carp fish until October 2021. The rank order of radioisotopes in fillet tissue carp fish was 40K (103.49 Bq kg-1) > 210Po (9.39 Bq kg-1) > 226Ra (0.62 Bq kg-1) > 230Th (0.39 Bq kg-1). The highest effective dose due to 210Po ingestion was observed in Spain (male; 4.44E-05 Sv y-1, female; 2.67E-06 Sv y-1); 40K (female, 5.07E-07 Sv y-1); 226Ra (male, 9.93E-09 Sv y-1). The mean of effective dose (ED) in the male and females in India due to ingestion of 230Th as result of carp fish consumption was (1.70E-06 Sv y-1) and (7.01E-08 Sv y-1), respectively. The probabilistic exposure assessment by the Monte Carlo simulation method revealed that consumers of fillet tissue carp fish content of radioisotopes are at a safe range (0.001 Sv y-1).

Probiotic bacteria alleviate chlorpyrifos-induced rat testicular and renal toxicity: A possible mechanism based on antioxidant and anti-inflammatory activity.

Chlorpyrifos (CPF) has caused many potential toxicities in nontarget organisms. Fewer studies have been conducted on the effects of lactic acid bacteria (LAB) in mitigating tissue damage induced by CPF in vivo. Therefore, we investigated CPF renal and testicular toxicity and the alleviating effect of probiotic lactobacilli, based on antioxidant and anti-inflammatory activity, on induced toxicity in an animal model. Biochemical assays showed that CPF induced oxidative stress along with a change in superoxide dismutase (SOD) and catalase (CAT) activity in a tissue-dependent manner. After treatment with CPF, testicular and renal levels of TNF-α were significantly reduced and enhanced, respectively, compared to the control group. The probiotic treatment restored renal and testicular TNF-α levels and modulated and blocked the increasing effect of CPF on renal IL-1ß levels. Testicular IL-1ß levels in the probiotic-treated and CPF groups demonstrated similar values. Exposure to CPF significantly induced renal histopathological damage that, of course, was completely inhibited by treatment with Lactobacillus casei and the LAB mixture. In summary, CPF showed significant toxicological effects on oxidative stress and the inflammation rate in CPF-exposed rats. Therefore, supplementation with probiotic bacteria may alleviate CPF renal toxicity and mitigate its oxidative stress and inflammation effects.

Concentration ciguatoxins in fillet of fish: A global systematic review and meta-analysis.

In the current study, an attempt was made to meta-analyze and discuss the concentration of ciguatoxins (CTXs) in fillets of fish based on country and water resources subgroups. The search was conducted in Scopus and PubMed, Embase and Web of Science to retrieve papers about the concentration of CTXs in fillet fish until July 2022. Meta-analysis concentration of CTXs was conducted based on countries and water resources subgroups in the random effects model (REM). The sort of countries based on the pooled concentration of CTXs was Kiribati (3.904 µg/kg) > Vietnam (1.880 µg/kg) > Macaronesia (1.400 µg/kg) > French (1.261 µg/kg) > China (0.674 µg/kg) > Japan (0.572 µg/kg) > USA (0.463 µg/kg) > Spain (0.224 µg/kg) > UK (0.170 µg/kg) > Fiji (0.162 µg/kg) > Mexico (0.150 µg/kg) > Australia (0.138 µg/kg) > Portugal (0.011 µg/kg). CTXs concentrations in all countries are higher than the safe limits of CTX1C (0.1 µg/kg). However, based on the safe limits of CTX1P, the concentrations of CTXs in just Portugal meet the regulation level (0.01 µg/kg). The minimum and maximum concentrations of CTXs were as observed in Selvagens Islands (0.011 µg/kg) and St Barthelemy (7.875 µg/kg) respectively. CTXs concentrations in all water resources are higher than safe limits of CTX1C (0.1 µg/kg) and CTX1B (0.01 µg/kg). Therefore, it is recommended to carry out continuous control pans of CTXs concentration in fish in different countries and water sources.

The occurrence of aflatoxin M1 in milk samples of Iran: a systematic review and meta-analysis.

In this study, the average level of aflatoxin M1 in various types of milk from 107 articles (297 studies with 16,274 milk samples) were meta-analyzed using random-effect model based on the milk varieties (animal species and heating processes), geographical regions, seasons, detection techniques and dairy farming subgroups. Studies on milk contamination with aflatoxin M1 in Iran were collected using universal and Persian databanks from January 1974 to the end of November 2021. The overall aflatoxin M1 mean concentration and prevalence in milk samples of Iran were 39.65 ng/l (95% CI: 36.00-43.30) and 80% (95% CI: 76-85%), respectively. The rank order of importance of various variables in mean levels of aflatoxin M1 in milk samples included milk type (animal species) > geographical regions > detection techniques > dairy farming types > milk types (heating processes) > seasons. Findings revealed that the overall content of aflatoxin M1 in milk samples of Iran was lower than that allowed by the European Union, Institute of Standards and Industrial Research of Iran, and the USA, possibly due to the milk monitoring by the Iranian regulatory systems.

Studying the microbial, chemical, and sensory characteristics of shrimp coated with alginate sodium nanoparticles containing Zataria multiflora and Cuminum cyminum essential oils.

Retardation of quality loss of seafood has been a new concept in recent years. This study's main objective was to evaluate the microbial, chemical, and sensory attributes of shrimp coated with alginate sodium nanoparticles containing Zataria multiflora and Cuminum cyminum essential oils (EOs) during refrigerated storage. At the end of storage time (15 days storage at 4°C), the pH, thiobarbituric acid reactive substances (TBARS), and total volatile basic nitrogen (TVBN) amounts in shrimps coated with the alginate nanoparticles were 7.62, 1.14 mg MDA/kg, and 117 mg/100 g which were significantly (p < .05) lower than the control groups. The count of all bacteria groups was also lower in this treatment, which was 2-2.74 Log CFU/mL on day 15 of cold storage. This combined treatment also obtained the highest sensory scores (around 7) and the lowest melanosis score (2.67) due to the effective delaying microbial and oxidation activities. Therefore, this edible coating could substantially retard microbial and chemical changes and improve the organoleptic properties of shrimp under refrigerated storage.

Research progress in the application of emerging technology for reducing food allergens as a global health concern: A systematic review.

Since the turn of the century, innovative food processing techniques have quickly risen to the top of the commercial and economic prominence food industry's priority list due to their many benefits over more conventional approaches. Compared to traditional food processing techniques, these innovative procedures retain better the distinctive aspects of food, including its organoleptic and nutritional attributes. Concurrently, there has been a discernible increase in the number of people, particularly infants and young children, who are allergic to certain foods. Although this is widely associated with shifting economic conditions in industrialized and developing countries, the rise of urbanization, the introduction of new eating patterns, and developments in food processing, it still needs to be determined how exactly these factors play a part. Under this circumstance, given the widespread presence of allergens that cause IgE-mediated reactions, it is critical to understand how the structural changes in protein as food is processed to determine whether the specific processing technique (conventional and novel) will be appropriate. This article discusses the impact of processing on protein structure and allergenicity and the implications of current research and methodologies for developing a platform to study future pathways to decrease or eliminate allergenicity in the general population.

The association between dietary acrylamide intake and the risk of type 2 diabetes incidence in the Tehran lipid and glucose study.

This study aimed at investigating the association of acrylamide consumption with the incidence of type 2 diabetes (T2D) in adults. The 6022 subjects of the Tehran lipid and glucose study participants were selected. The acrylamide content of food items were summed and computed cumulatively across follow up surveys. Multivariable Cox proportional hazard regression analyses were performed to estimate the hazards ratio (HR) and 95% confidence interval (CI) of incident T2D. This study was done on men and women, respectively aged 41.5 ± 14.1 and 39.2 ± 13.0 years. The mean ± SD of dietary acrylamide intake was 57.0 ± 46.8 µg/day. Acrylamide intake was not associated with the incidence of T2D after adjusting for confounding variables. In women, a higher acrylamide intake was positively associated with T2D [HR (CI) for Q4: 1.13 (1.01-1.27), P trend: 0.03] after adjusting for confounding factors. Our results demonstrated that dietary intake of acrylamide was associated with an increased risk of T2D in women.

Application of inulin in bread: A review of technological properties and factors affecting its stability.

Due to its dual function, inulin is an important prebiotic compound in the cereal industry, especially in bread production. In other words, improving technological features and creating health properties (such as reducing the risk of type 2 diabetes, heart disease, metabolic syndrome, and osteoporosis) have led to the widespread use of this compound. Inulin has many important technological functions in bread, including its ability to interact with water, create structure, and influence rheological properties, texture, and overall acceptability of the final product. Nevertheless, bread processing conditions can influence the structural integrity of inulin and thus affect its technological efficiency. Therefore, this review article aims to investigate the technological properties and factors affecting the stability of inulin during bread processing conditions. Generally, the addition of inulin could considerably improve the technical performance of bread. However, the stability of inulin depends on the formulation components, type of fermentation, and baking process.

The Concentration of Radionuclides (Lead-210, Polonium-210, and Cesium-137) in the Muscle of Sardine Fish: a Global Systematic Review, Meta-analysis, and Exposure Assessment.

With the increasing use of nuclear technology, concerns about the contamination of water resources with radionuclides and the contamination of seafood are increasing. In the current investigation, a meta-analysis and exposure assessment regarding the concentration of radionuclides, including lead-210 (Pb-210), polonium -210 (Po-210), and cesium-137 (Cs-137) in the muscle of Sardin fish were conducted. In this regard, some databases including Scopus and PubMed were selected to retrieve papers on the concentration of radionuclides in the muscle of Sardin fish from January 2000 to 25 June 2021. The used keywords were polonium-210, natural radioactivity, lead-210, radiocesium, cesium-137, radionuclides, radium-226, seafood, marine foods, fish, sardine fish, sardinella longiceps, and Clupeidae. Also, effective dose (ED) was calculated to estimate carcinogenic risk in Sardine fish consumers. The rank order of radionuclides in the muscle of sardine fish was Po-210 (31.50 Bq/kg) > Pb-210 (3.34 Bq/kg) > Cs-137 (0.48 Bq/kg). Based on level of Pb-210 rank of countries was France (12.00 Bq/kg) > India (4.06 Bq/kg) > Turkey (3.29 Bq/kg) > Spain (1.00 Bq/kg) > Slovenia (0.55 Bq/kg); Po-210 was Turkey (74.96 Bq/kg) > Spain (48.00 Bq/kg) > France (31.500 Bq/kg > India (30.25 Bq/kg) > Slovenia (25.00 Bq/kg) > Kuwait (7.28 Bq/kg) and also, Cs-137 was Japan (1.22 Bq/kg) > Croatia (0.18 Bq/kg) > India (0.23 Bq/kg). The lowest and highest ED of 210Po was observed in Kuwait (5.10E-04 msv/y, female) and France (3.14E-01, male); Pb-210, Slovenia (7.24E-05 msv/y, male) and France (1.48E-02 msv/y, female), and Cs-137, Croatia (8.47E-08 msv/y, male) and Japan (2.54E-05 msv/y, female), respectively. The risk assessment shows that the exposed population are at an acceptable range of risk (H < 1 mSv/y).

Potentially toxic elements (PTEs) in coffee: a comprehensive review of toxicity, prevalence, and analytical techniques.

Coffee is one of the most popular non-alcoholic beverages, consumed as a raw material in different food sectors. The popularity of coffee is induced by its pleasant flavor, taste, and highly nutritious nature. However, the absorption of potentially toxic elements (PTEs) through preharvest, harvesting, and post-harvest stages makes it a potentially rich source. Essential elements are potentially toxic at a higher concentration than required for the human body to work. PTEs intake through food systems may lead to health risks, including mutagenicity, teratogenicity, carcinogenicity, and embryotoxic effects. Different analytical techniques such as spectroscopy, electromigration, and electrochemical. are used for PTEs content determination of coffee. Considering the importance of PTEs in human health and the worldwide popularity of coffee, their monitoring of coffee is crucial. Therefore, this study is aimed to investigate the classification, prevalence, and determination techniques of PTEs in different coffee types.

The concentration of potentially toxic elements (PTEs) in the coffee products: a systematic review and meta-analysis.

Coffee is one of the most consumed products globally, and its contamination with potentially toxic elements (PTEs) occurs throughout the production chain and production. Therefore, the current meta-analysis study aimed to estimate the concentration of essential elements (Cu and Co) and the contamination of PTEs (Ni, Cr, Pb, As, and Cd) in coffee. The recommended databases, including PubMed, Scopus, and ScienceDirect, were investigated to collect data regarding the contamination of PTEs in coffee products from 2010 to 2021. Among 644 retrieved citations in the identification step, 34 articles were included in the meta-analysis. The pooled mean concentration of essential elements in coffee products is much higher than that of toxic elements (Co (447.106 µg/kg, 95% CI: 445.695-448.518 µg/kg) > Ni (324.175 µg/kg, 95% CI: 322.072-326.278 µg/kg) > Cu (136.171 µg/kg, 95% CI: 134.840-137.503 µg/kg) > Cr (106.865 µg/kg, 95% CI: 105.309-108.421 µg/kg) > Pb (21.027 µg/kg, 95% CI: 20.824-21.231 µg/kg) > As (3.158 µg/kg, 95% CI: 3.097-3.219 µg/kg) > Cd (0.308 µg/kg; 95% CI: 0.284-0.332 µg/kg)). Results showed high differences between pooled concentrations of all PTEs in coffee products of different countries.

Nanoemulsion and nanogel containing Artemisia dracunculus essential oil; larvicidal effect and antibacterial activity.

OBJECTIVE: Microbial infections and mosquito-borne diseases such as malaria, with 627 k deaths in 2020, are still major public health challenges. RESULTS: This study prepared nanoemulsion and nanogel containing Artemisia dracunculus essential oil. ATR-FTIR analysis (Attenuated Total Reflection-Fourier Transform InfraRed) confirmed the successful loading of the essential oil in nanoemulsion and nanogel. LC50 values (Lethal Concentration 50%) of nanogel and nanoemulsion against Anopheles stephensi larvae were obtained as 6.68 (2-19 µg/mL) and 13.53 (7-25 µg/mL). Besides, the growth of Staphylococcus aureus after treatment with 5000 µg/mL nanogel and nanoemulsion was reduced by ~ 70%. However, about 20% growth of Pseudomonas aeruginosa was reduced at this dose. Considering the proper efficacy of the nanogel as a larvicide and proper antibacterial effect against S. aureus, it could be considered for further investigations against other mosquitoes' larvae and gram-positive bacteria.

Novel nonthermal food processing practices: Their influences on nutritional and technological characteristics of cereal proteins.

Cereals, as the main crops cultivated and consumed in the world, are a rich source of carbohydrates, proteins, dietary fiber, and minerals. Despite the nutritional importance, their technological applicability in food matrices is also considerably important to be determined. Cereal processing is done to achieve goals as increasing the shelf-life, obtaining the desired technological function, and enhancing the nutritional value. Nonthermal processing is preferred regarding its potential to provide beneficial impacts with minimum adverse effect. Technological functionality and nutritional performance are considered as the most basic challenges through cereal processing, with proteins as the main factor to take part in such roles. Technological and nutritional functionalities of proteins have been found to be changed through nonthermal processing, which is generally attributed to conformational and structural changes. Therefore, this study is aimed to investigate the impact of nonthermal processing on nutritional and technological characteristics of cereal proteins.

Antimicrobial Activity of Films and Coatings Containing Lactoperoxidase System: A Review.

The production of safe and healthy foodstuffs is considered as one of the most important challenges in the food industry, and achieving this important goal is impossible without using various processes and preservatives. However, recently, there has been a growing concern about the use of chemical preservatives and attention has been focused on minimal process and/or free of chemical preservatives in food products. Therefore, researchers and food manufacturers have been induced to utilize natural-based preservatives such as antimicrobial enzymes in their production. Lactoperoxidase, as an example of antimicrobial enzymes, is the second most abundant natural enzyme in the milk and due to its wide range of antibacterial activities, it could be potentially applied as a natural preservative in various food products. On the other hand, due to the diffusion of lactoperoxidase into the whole food matrix and its interaction and/or neutralization with food components, the direct use of lactoperoxidase in food can sometimes be restricted. In this regard, lactoperoxidase can be used as a part of packaging material, especially edible and coating, to keep its antimicrobial properties to extend food shelf-life and food safety maintenance. Therefore, this study aims to review various antimicrobial enzymes and introduce lactoperoxidase as a natural antimicrobial enzyme, its antimicrobial properties, and its functionality in combination with an edible film to extend the shelf-life of food products.

Diazinon reduction in food products: a comprehensive review of conventional and emerging processing methods.

Diazinon is known as one of the most commonly used organophosphorus pesticides which influence different pests through inactivating acetyl choline esterase enzymes. Despite diazinon applications, its toxicity to human health could result in a worldwide concern about its occurrence in foodstuffs. Malfunction of brain is considered as the main disorders induced by long time exposure to diazinon. Due to the degradation of diazinon in high temperatures and its susceptibility to oxidation as well as acidic and basic conditions, it could be degraded through several physical (9-94%) and chemical (19.3-100%) food processing procedures (both household and industrial methods). However, each of these methods has its advantages and disadvantages. Normally, the combination of these methods is more efficient in diazinon reduction. To this end, it is important to apply an effective method for diazinon reduction in the food products without affecting food quality or treating human health. It could be noticed that bioremediation by microorganisms such as probiotics could be a promising new method for diazinon's reduction in several food products.

Subacute toxicity of chlorpyrifos on histopathological damages, antioxidant activity, and pro-inflammatory cytokines in the rat model.

Chlorpyrifos (CPF) is an extensively used organophosphorus pesticide for agricultural, industrial, and domestic purposes. Previous studies have reported the adverse effects of CPF, such as intoxication incidents, endocrine disruption, cardiovascular diseases, as well as histopathological and oxidative damage. The aims of the present study were to elucidate short time subacute toxicity of CPF in male rats. Sprague-Dawley male rats (n = 32) were divided into four groups (n = 8) and received CPF as 3.25 mg/kg body weight (b.w) (Group A), 6.75 mg/kg b.w (Group B), 13.5 mg/kg b.w (Group C), and corn oil (control or Group D) daily via gavage for 15 days. The rats were sacrificed and oxidative damages, pro-inflammatory cytokines (TNF-α, IL-1ß), and histopathological changes were determined in the lung, liver, kidney, heart, and testis tissues as well as plasma. According to our result, administration of CPF caused a significant increase in malondialdehid level and catalase activity while a significant decrease in superoxide dismutase activity in all tissues. In addition, a significant decrease in TNF-α observed in all tissues and plasma duo to the CPF. Histopathological evaluation of CPF-treated samples revealed a dose-dependent tissue toxicity in the liver, heart, lung, and kidney with less sensitivity of testicular and kidney tissues. These results suggest the potential of CPF in inducing oxidative stress at low doses and short duration time with similar trends in different tissues. As well as, due to the effects of CPF on some pro-inflammatory mediators, more comprehensive studies are recommended.

Occurrence of Aflatoxins in Commercial Cereal-based Baby Foods in Iran: A Probabilistic Risk Assessment to Health.

The aim of this study was to assess the occurrence of Aflatoxins (AFs) including B1, B2, G1 and G2 in commercial cereal-based baby foods by HPLC-FLD method in Iran and related risk assessment in three baby age groups (6-12, 12-18, and 18-24 months) using Monte Carlo simulation approach. Results showed an occurrence ranging from 20% to 60% for B1, B2, and G2 aflatoxins, while AFG1 was not detected in any assessed samples. Exposure and risk assessment was estimated to be two groups (aflatoxin B1 and total aflatoxins). The highest estimated dietary exposure to both AFB1 and total AFs was estimated for 6-12 months aged babies, representing 5.81 ng/kg BW/day and 8.55 ng/kg BW/day, respectively. Overall, the margin of exposures to AFB1 and total AFs were lower than 10,000 in all age groups, indicating a health concern about AFB1 and total AFs exposure through cereal-based baby food consumption. High cancer risk for high consumers (P95) of baby food was also estimated in all age groups, calling for immediate intervention due to serious claims that AFB1, is a highly carcinogenic component, causes hepatocellular carcinoma. Risk ranking results indicated the presence of AFB1 is classified as high risk for babies who consume cereal-based foods, which demands the attention of risk managers to reduce or eliminate this risk for the most vulnerable sector of society, whose aged <24 months.

A global systematic review and meta-analysis of concentration and prevalence of mycotoxins in birds' egg.

In the current study, the concentration and prevalence of birds' egg's mycotoxins among 11 articles (66 studies) based on countries, part of eggs, and type of mycotoxins subgroups were meta-analyses using a random-effect model. The order of mycotoxin according to concentration of mycotoxin was Deoxynivalenol (20.083 µg/kg) > Zearalenone (2.065 µg/kg) > Enniatin (1.120 µg/kg) > Total aflatoxin (0.371 µg/kg) > Beauvericin (0.223 µg/kg) > Ochratoxins (0.087 µg/kg) > Citrinin (0.010 µg/kg). Further, the mycotoxins' concentration in the yolk part (2.070 µg/kg) was higher than the mixed eggs (0.283 µg/kg). The rank order of mycotoxin based on country was China (14.990 µg/kg) > Cameroon (7.594 µg/kg) > Thailand (1.870 µg/kg) > Finland (0.920 µg/kg) > Iran (0.312 µg/kg) > Jordan (0.202 µg/kg) > Belgium (0.183 µg/kg) > Spain ( South Korea ( DON (85.00%) > AFT (20.15%) > OT (16.00%). The overall prevalence of mycotoxin was equal to 29.65%. Also, the concentration of mycotoxins in China and Cameroon was higher than in other countries. Therefore, the monitoring programs to reduce mycotoxins in bird eggs consumed in some countries such as China and Cameroon should be considered.

Improving the oxidative stability of virgin olive oil using microformulated vitamin-C.

This study aims to improve the oxidative stability of olive oil using microformulated vitamin-C (Vit-C). The microemulsion containing 10,000 µg/ml Vit-C with a droplet size of 1,000 ± 68 nm was first prepared. Free radical scavenging of olive oil and olive oil containing blank microemulsion, different amounts of formulated Vit-C (100-500 µg/ml), and TBHQ (100 and 200 µg/ml as a standard antioxidant) was around 90% during 21 days of incubation at 60°C. The oxidative stability of the mentioned samples during incubation was investigated using the rancimat test, and their quality criteria analysis was studied by peroxide and the acid values. Results showed that the sample's acid value containing 500 µg/ml of Vit-C did not show significant differences (p < .05) with samples containing TBHQ. However, samples containing TBHQ's peroxide value were significant (p < .05) lower than samples containing 500 µg/ml of Vit-C. Furthermore, the induction time of samples containing 500 µg/ml of Vit-C was significantly (p < .05) higher than other treatments during incubation. Thus, the prepared microemulsion could be used as a natural antioxidant in the oil industry instead of harmful synthetic TBHQ.