RESUMO
To determine the effects of ingested royal jelly (RJ) on the pituitary in middle-aged female rats, we performed a long-term RJ administration test. Several animals showed age-related increases in pituitary weight, and RJ administration compensated for the increase. RJ tended to down-regulate prolactin mRNA and up-regulated thyroid-stimulating hormone beta mRNA in the pituitary. This suggests that RJ compensates for age-associated decline in pituitary functions.
Assuntos
Envelhecimento , Ácidos Graxos/administração & dosagem , Ácidos Graxos/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Hipófise/anatomia & histologia , Hipófise/efeitos dos fármacos , Envelhecimento/efeitos dos fármacos , Envelhecimento/genética , Animais , Feminino , Tamanho do Órgão/efeitos dos fármacos , Hormônios Hipofisários/genética , Prolactina/sangue , Prolactina/genética , Ratos , Ratos Sprague-Dawley , Tiroxina/sangue , Fatores de TempoRESUMO
Administration of short-chain fructooligosaccharide (scFOS) is known to lower serum triglyceride levels in rats fed a high-fat diet, but the molecular mechanisms remain unclear. This study aimed to identify marker genes for lipid-lowering effect of scFOS administration. The changes in hepatic gene expressions in rats fed scFOS were investigated using DNA microarray and quantitative RT-PCR analysis. The DNA microarray showed that phytanoyl-CoA 2-hydroxylase 2 (Phyh2), lipoprotein lipase (Lpl) and tyrosine aminotransferase (Tat) were significantly affected by scFOS administration (p < .05). Since Lpl is involved in lipid metabolism, the up-regulation of Lpl in the liver can be a potential marker of the lipid-lowering effect of scFOS.
Assuntos
Inibidores Enzimáticos/farmacologia , Enzimas/genética , Frutose/farmacologia , Hipolipemiantes/farmacologia , Fígado/efeitos dos fármacos , Oligossacarídeos/farmacologia , Triglicerídeos/genética , Animais , Coenzima A/metabolismo , DNA/análise , Enzimas/metabolismo , Expressão Gênica/efeitos dos fármacos , Marcadores Genéticos , Metabolismo dos Lipídeos/efeitos dos fármacos , Lipase Lipoproteica/genética , Lipase Lipoproteica/metabolismo , Fígado/metabolismo , Masculino , Análise em Microsséries , Oxigenases de Função Mista/genética , Oxigenases de Função Mista/metabolismo , Ácido Fitânico/análogos & derivados , Ácido Fitânico/metabolismo , Ratos Wistar , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Triglicerídeos/sangue , Triglicerídeos/metabolismo , Tirosina Transaminase/genética , Tirosina Transaminase/metabolismo , Regulação para CimaRESUMO
This study investigated the gender differences in the kidney function of magnesium (Mg)-deficient rats. Male and female rats were fed a control diet or a Mg-deficient diet for 21 d. Mg-deficient diet had no significant effect on kidney calcium (Ca) or phosphorus (P) concentration in male rats, while Ca and P concentrations in female rats were significantly higher in Mg-deficient rats than in the control rats. With regard to indicators of kidney function, no significant differences in creatinine clearance and serum urea nitrogen concentration were observed among the groups. Serum albumin concentrations were significantly lower in rats fed the Mg-deficient diet than in rats fed the control diet. In both sexes, urinary albumin excretion was significantly higher in rats fed the Mg-deficient diet than in rats fed the control diet. Gender differences had no significant influence on creatinine clearance, serum urea nitrogen concentration, serum albumin concentration and urinary albumin excretion. These results suggest that gender differences have no effect on kidney function in Mg-deficient rats under the condition used.